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Protein

Carbohydrate-responsive element-binding protein

Gene

Mlxipl

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transcriptional repressor. Binds to the canonical and non-canonical E box sequences 5'-CACGTG-3'.

GO - Molecular functioni

  • carbohydrate response element binding Source: BHF-UCL
  • protein heterodimerization activity Source: MGI
  • RNA polymerase II core promoter proximal region sequence-specific DNA binding Source: NTNU_SB
  • transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding Source: NTNU_SB
  • transcription factor binding Source: BHF-UCL

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Repressor

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

ReactomeiR-MMU-163358. PKA-mediated phosphorylation of key metabolic factors.
R-MMU-163765. ChREBP activates metabolic gene expression.

Names & Taxonomyi

Protein namesi
Recommended name:
Carbohydrate-responsive element-binding protein
Short name:
ChREBP
Alternative name(s):
MLX interactor
MLX-interacting protein-like
Williams-Beuren syndrome chromosomal region 14 protein homolog
Gene namesi
Name:Mlxipl
Synonyms:Mio, Wbscr14
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:1927999. Mlxipl.

Subcellular locationi

  • Nucleus PROSITE-ProRule annotation

GO - Cellular componenti

  • cytoplasm Source: BHF-UCL
  • cytosol Source: Reactome
  • nucleoplasm Source: Reactome
  • nucleus Source: BHF-UCL
  • transcription factor complex Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 864864Carbohydrate-responsive element-binding proteinPRO_0000127505Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei20 – 201PhosphoserineCombined sources
Modified residuei23 – 231PhosphoserineCombined sources
Modified residuei25 – 251PhosphoserineCombined sources
Modified residuei27 – 271PhosphothreonineCombined sources
Modified residuei196 – 1961PhosphoserineBy similarity
Modified residuei566 – 5661Phosphoserine; by AMPKBy similarity
Modified residuei614 – 6141PhosphoserineBy similarity
Modified residuei626 – 6261PhosphoserineCombined sources
Modified residuei643 – 6431PhosphoserineCombined sources

Post-translational modificationi

Phosphorylation at Ser-566 by AMPK inactivates the DNA-binding activity.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ99MZ3.
MaxQBiQ99MZ3.
PaxDbiQ99MZ3.
PeptideAtlasiQ99MZ3.
PRIDEiQ99MZ3.

PTM databases

iPTMnetiQ99MZ3.
PhosphoSiteiQ99MZ3.

Expressioni

Tissue specificityi

Expressed in the ventricular and intermediate zones of the developing spinal cord of E12.5 embryos. In later embryos expressed in a variety of tissues.

Gene expression databases

BgeeiQ99MZ3.
CleanExiMM_MLXIPL.
ExpressionAtlasiQ99MZ3. baseline and differential.
GenevisibleiQ99MZ3. MM.

Interactioni

Subunit structurei

Binds DNA as a heterodimer with TCFL4/MLX.

GO - Molecular functioni

  • protein heterodimerization activity Source: MGI
  • transcription factor binding Source: BHF-UCL

Protein-protein interaction databases

BioGridi208443. 6 interactions.
IntActiQ99MZ3. 1 interaction.
MINTiMINT-4140243.
STRINGi10090.ENSMUSP00000005507.

Structurei

Secondary structure

1
864
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi118 – 13417Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4GNTX-ray2.41B117-137[»]
ProteinModelPortaliQ99MZ3.
SMRiQ99MZ3. Positions 670-716.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini661 – 71555bHLHPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni715 – 73622Leucine-zipperAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi345 – 3506Poly-Ser

Sequence similaritiesi

Contains 1 bHLH (basic helix-loop-helix) domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG3582. Eukaryota.
ENOG410XTA5. LUCA.
GeneTreeiENSGT00530000063219.
HOGENOMiHOG000113606.
HOVERGENiHBG073589.
InParanoidiQ99MZ3.
KOiK09113.
OMAiWRPPERW.
OrthoDBiEOG7ZD1VF.
PhylomeDBiQ99MZ3.
TreeFamiTF324749.

Family and domain databases

Gene3Di4.10.280.10. 1 hit.
InterProiIPR011598. bHLH_dom.
[Graphical view]
PfamiPF00010. HLH. 1 hit.
[Graphical view]
SMARTiSM00353. HLH. 1 hit.
[Graphical view]
SUPFAMiSSF47459. SSF47459. 1 hit.
PROSITEiPS50888. BHLH. 1 hit.
[Graphical view]

Sequences (5)i

Sequence statusi: Complete.

This entry describes 5 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q99MZ3-1) [UniParc]FASTAAdd to basket

Also known as: Zeta

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MARALADLSV NLQVPRVVPS PDSDSDTDLE DPSPRRSAGG LHRSQVIHSG
60 70 80 90 100
HFMVSSPHSD SLTRRRDQEG PVGLADFGPR SIDPTLTHLF ECLSLAYSGK
110 120 130 140 150
LVSPKWKNFK GLKLLCRDKI RLNNAIWRAW YIQYVQRRKS PVCGFVTPLQ
160 170 180 190 200
GSEADEHRKP EAVILEGNYW KRRIEVVMRE YHKWRIYYKK RLRKSSREGD
210 220 230 240 250
FLAPKQVEGG WPPPERWCEQ LFSSVVPVLL GGSEEEPGGR QLLDLDCFLS
260 270 280 290 300
DISDTLFTMT QPSPSSLQLP PEDAYVGNAD MIQPDLTPLQ PSLDDFMEIS
310 320 330 340 350
DFFTNYRPPQ TPTSSNYIES PSFGPMADSL FSSGILAPEM PSPASSSSSS
360 370 380 390 400
GMTPHSGNTR LQARNSCSGP LDPNPFLSSE FLLPEDPKTK IPPAPGPTPL
410 420 430 440 450
LPFPTPVKVH GLEPCTPSPF PTMAPPPSLL PEESLLSARF PFTSAPPAPG
460 470 480 490 500
VSTLPAPTTF VPTPQPGPGP VPFSVDHLPH GYLEPVFGPH FTVPQGMQPR
510 520 530 540 550
CKPSSPSPGG QKASPPTLAS ATASPTATAT ARDNNPCLTQ LLRAAKPEQA
560 570 580 590 600
LEPPTMPGTL LRPPESPQDT VSEIPRARAF FPPIPAPTPP RPPPGPATLA
610 620 630 640 650
PPRSLVVPKA ERLSPPASSG SERRLSGDLN SIQPSGALSV HLSPPQTVLS
660 670 680 690 700
RGRVDNNKME NRRITHISAE QKRRFNIKLG FDTLHGLVST LSAQPSLKVS
710 720 730 740 750
KATTLQKTAE YILMLQQERA AMQEEAQQLR DEIEELNAAI NLCQQQLPAT
760 770 780 790 800
GVPITHQRFD QMRDMFDDYV RTRTLHNWKF WVFSILIRPL FESFNGMVST
810 820 830 840 850
ASLHSLRQTS LAWLEQYCSL PALRPTVLNS LRQLSTSTSI LTDPSLVPEQ
860
ATRAVTEGTL GRPL
Length:864
Mass (Da):94,875
Last modified:June 1, 2001 - v1
Checksum:i7E6AFFB04C71B327
GO
Isoform 2 (identifier: Q99MZ3-2) [UniParc]FASTAAdd to basket

Also known as: Theta

The sequence of this isoform differs from the canonical sequence as follows:
     58-79: Missing.

Show »
Length:842
Mass (Da):92,482
Checksum:i14BFA5F3820AFF74
GO
Isoform 3 (identifier: Q99MZ3-3) [UniParc]FASTAAdd to basket

Also known as: Iota

The sequence of this isoform differs from the canonical sequence as follows:
     699-744: VSKATTLQKT...ELNAAINLCQ → GLPTQRPTLV...YAGGGAAAAG
     745-864: Missing.

Show »
Length:744
Mass (Da):80,170
Checksum:iF052B12D7CE0259B
GO
Isoform 4 (identifier: Q99MZ3-4) [UniParc]FASTAAdd to basket

Also known as: Kappa

The sequence of this isoform differs from the canonical sequence as follows:
     699-714: VSKATTLQKTAEYILM → LPGLANTEAHIGGARR
     715-864: Missing.

Show »
Length:714
Mass (Da):77,556
Checksum:i4BAD0BE936A72292
GO
Isoform 5 (identifier: Q99MZ3-5) [UniParc]FASTAAdd to basket

Also known as: Eta

The sequence of this isoform differs from the canonical sequence as follows:
     545-556: AKPEQALEPPTM → VVLIVLPVPSQA
     557-864: Missing.

Show »
Length:556
Mass (Da):60,602
Checksum:iBF8BA1D9BEA7E942
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti67 – 671D → Y in AAF68175 (PubMed:10780788).Curated
Sequence conflicti107 – 1071K → N in AAF68175 (PubMed:10780788).Curated
Sequence conflicti128 – 1281R → I in AAF68175 (PubMed:10780788).Curated
Sequence conflicti138 – 1392RK → TR in AAF68175 (PubMed:10780788).Curated
Sequence conflicti155 – 1551D → H in AAF68175 (PubMed:10780788).Curated
Sequence conflicti175 – 1751E → D in AAF68175 (PubMed:10780788).Curated
Sequence conflicti183 – 1831K → V in AAF68175 (PubMed:10780788).Curated
Sequence conflicti727 – 7282QQ → HE in AAF68175 (PubMed:10780788).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei58 – 7922Missing in isoform 2. CuratedVSP_002174Add
BLAST
Alternative sequencei545 – 55612AKPEQ…EPPTM → VVLIVLPVPSQA in isoform 5. CuratedVSP_002175Add
BLAST
Alternative sequencei557 – 864308Missing in isoform 5. CuratedVSP_002176Add
BLAST
Alternative sequencei699 – 74446VSKAT…INLCQ → GLPTQRPTLVALAGEQSNHA SEDSGVHPDAAAGTGSYAGG GAAAAG in isoform 3. CuratedVSP_002177Add
BLAST
Alternative sequencei699 – 71416VSKAT…EYILM → LPGLANTEAHIGGARR in isoform 4. CuratedVSP_002179Add
BLAST
Alternative sequencei715 – 864150Missing in isoform 4. CuratedVSP_002180Add
BLAST
Alternative sequencei745 – 864120Missing in isoform 3. CuratedVSP_002178Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF245475 mRNA. Translation: AAK20940.1.
AF245476 mRNA. Translation: AAK20941.1.
AF245477 mRNA. Translation: AAK20942.1.
AF245478 mRNA. Translation: AAK20943.1.
AF245479 mRNA. Translation: AAK20944.1.
AF156604 mRNA. Translation: AAF68175.1.
CCDSiCCDS39316.1. [Q99MZ3-1]
RefSeqiNP_067430.2. NM_021455.4. [Q99MZ3-1]
UniGeneiMm.34213.

Genome annotation databases

EnsembliENSMUST00000005507; ENSMUSP00000005507; ENSMUSG00000005373. [Q99MZ3-1]
ENSMUST00000128691; ENSMUSP00000121348; ENSMUSG00000005373. [Q99MZ3-4]
ENSMUST00000129008; ENSMUSP00000114933; ENSMUSG00000005373. [Q99MZ3-5]
ENSMUST00000153519; ENSMUSP00000122198; ENSMUSG00000005373. [Q99MZ3-3]
GeneIDi58805.
KEGGimmu:58805.
UCSCiuc008zxt.2. mouse. [Q99MZ3-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF245475 mRNA. Translation: AAK20940.1.
AF245476 mRNA. Translation: AAK20941.1.
AF245477 mRNA. Translation: AAK20942.1.
AF245478 mRNA. Translation: AAK20943.1.
AF245479 mRNA. Translation: AAK20944.1.
AF156604 mRNA. Translation: AAF68175.1.
CCDSiCCDS39316.1. [Q99MZ3-1]
RefSeqiNP_067430.2. NM_021455.4. [Q99MZ3-1]
UniGeneiMm.34213.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4GNTX-ray2.41B117-137[»]
ProteinModelPortaliQ99MZ3.
SMRiQ99MZ3. Positions 670-716.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi208443. 6 interactions.
IntActiQ99MZ3. 1 interaction.
MINTiMINT-4140243.
STRINGi10090.ENSMUSP00000005507.

PTM databases

iPTMnetiQ99MZ3.
PhosphoSiteiQ99MZ3.

Proteomic databases

EPDiQ99MZ3.
MaxQBiQ99MZ3.
PaxDbiQ99MZ3.
PeptideAtlasiQ99MZ3.
PRIDEiQ99MZ3.

Protocols and materials databases

DNASUi58805.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000005507; ENSMUSP00000005507; ENSMUSG00000005373. [Q99MZ3-1]
ENSMUST00000128691; ENSMUSP00000121348; ENSMUSG00000005373. [Q99MZ3-4]
ENSMUST00000129008; ENSMUSP00000114933; ENSMUSG00000005373. [Q99MZ3-5]
ENSMUST00000153519; ENSMUSP00000122198; ENSMUSG00000005373. [Q99MZ3-3]
GeneIDi58805.
KEGGimmu:58805.
UCSCiuc008zxt.2. mouse. [Q99MZ3-1]

Organism-specific databases

CTDi51085.
MGIiMGI:1927999. Mlxipl.

Phylogenomic databases

eggNOGiKOG3582. Eukaryota.
ENOG410XTA5. LUCA.
GeneTreeiENSGT00530000063219.
HOGENOMiHOG000113606.
HOVERGENiHBG073589.
InParanoidiQ99MZ3.
KOiK09113.
OMAiWRPPERW.
OrthoDBiEOG7ZD1VF.
PhylomeDBiQ99MZ3.
TreeFamiTF324749.

Enzyme and pathway databases

ReactomeiR-MMU-163358. PKA-mediated phosphorylation of key metabolic factors.
R-MMU-163765. ChREBP activates metabolic gene expression.

Miscellaneous databases

PROiQ99MZ3.
SOURCEiSearch...

Gene expression databases

BgeeiQ99MZ3.
CleanExiMM_MLXIPL.
ExpressionAtlasiQ99MZ3. baseline and differential.
GenevisibleiQ99MZ3. MM.

Family and domain databases

Gene3Di4.10.280.10. 1 hit.
InterProiIPR011598. bHLH_dom.
[Graphical view]
PfamiPF00010. HLH. 1 hit.
[Graphical view]
SMARTiSM00353. HLH. 1 hit.
[Graphical view]
SUPFAMiSSF47459. SSF47459. 1 hit.
PROSITEiPS50888. BHLH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "WBSCR14, a gene mapping to the Williams-Beuren syndrome deleted region, is a new member of the Mlx transcription factor network."
    Cairo S., Merla G., Urbinati F., Ballabio A., Reymond A.
    Hum. Mol. Genet. 10:617-627(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], CHARACTERIZATION, ALTERNATIVE SPLICING.
  2. "WBSCR14, a putative transcription factor gene deleted in Williams-Beuren syndrome: complete characterisation of the human gene and the mouse ortholog."
    de Luis O., Valero M.C., Perez Jurado L.A.
    Eur. J. Hum. Genet. 8:215-222(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-23 AND SER-25, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.
  4. "Specific phosphopeptide enrichment with immobilized titanium ion affinity chromatography adsorbent for phosphoproteome analysis."
    Zhou H., Ye M., Dong J., Han G., Jiang X., Wu R., Zou H.
    J. Proteome Res. 7:3957-3967(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-23; SER-25 AND THR-27, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.
  5. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-20; SER-23; SER-25; SER-626 AND SER-643, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brown adipose tissue, Kidney, Liver and Testis.

Entry informationi

Entry nameiMLXPL_MOUSE
AccessioniPrimary (citable) accession number: Q99MZ3
Secondary accession number(s): Q99MY9
, Q99MZ0, Q99MZ1, Q99MZ2, Q9JLM5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 23, 2002
Last sequence update: June 1, 2001
Last modified: July 6, 2016
This is version 133 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.