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Q99MY8 (ASH1L_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 101. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Histone-lysine N-methyltransferase ASH1L

EC=2.1.1.43
Alternative name(s):
ASH1-like protein
Absent small and homeotic disks protein 1 homolog
Gene names
Name:Ash1l
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length2958 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Histone methyltransferase specifically methylating 'Lys-36' of histone H3 (H3K36me) By similarity.

Catalytic activity

S-adenosyl-L-methionine + L-lysine-[histone] = S-adenosyl-L-homocysteine + N(6)-methyl-L-lysine-[histone].

Subcellular location

Nucleus By similarity. Cell junctiontight junction By similarity. Chromosome By similarity. Note: The relevance of tight junction localization is however unclear By similarity.

Sequence similarities

Belongs to the class V-like SAM-binding methyltransferase superfamily. Histone-lysine methyltransferase family. SET2 subfamily.

Contains 3 A.T hook DNA-binding domains.

Contains 1 AWS domain.

Contains 1 BAH domain.

Contains 1 bromo domain.

Contains 1 PHD-type zinc finger.

Contains 1 post-SET domain.

Contains 1 SET domain.

Sequence caution

The sequence AAK26242.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

The sequence BAE32182.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 29582958Histone-lysine N-methyltransferase ASH1L
PRO_0000259517

Regions

Domain2081 – 213252AWS
Domain2135 – 2251117SET
Domain2259 – 227517Post-SET
Domain2452 – 252271Bromo
Domain2650 – 2787138BAH
DNA binding885 – 89713A.T hook 1
DNA binding1345 – 135713A.T hook 2
DNA binding1843 – 185513A.T hook 3
Zinc finger2574 – 262047PHD-type
Region2059 – 2278220Catalytic domain By similarity
Compositional bias1378 – 142245Pro-rich
Compositional bias1555 – 1787233Ser-rich

Amino acid modifications

Modified residue221Phosphoserine By similarity
Modified residue3731N6-acetyllysine By similarity
Modified residue23071N6-acetyllysine Ref.6
Modified residue23091N6-acetyllysine Ref.6
Modified residue23131N6-acetyllysine Ref.6
Cross-link400Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) By similarity
Cross-link404Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) By similarity

Experimental info

Sequence conflict3311L → M in BAC40390. Ref.2
Sequence conflict711 – 7144RKGR → EKEE in AAK26242. Ref.4
Sequence conflict10731Q → K in AAK26242. Ref.4
Sequence conflict10951A → V in AAK26242. Ref.4
Sequence conflict19941Y → S in AAK26242. Ref.4
Sequence conflict26561D → H in AAK26242. Ref.4

Sequences

Sequence LengthMass (Da)Tools
Q99MY8 [UniParc].

Last modified July 27, 2011. Version 3.
Checksum: E8CA3E7AB72BEF2B

FASTA2,958331,333
        10         20         30         40         50         60 
MDPRNTAMLG LGSDSEGFSR KSPSTINPGT LASKREAEIE GATEEEDPRK RNRERGTEAG 

        70         80         90        100        110        120 
KEDGSTDAQQ QFSVKETNFS EGNLKLKIGL QAKRTKKPPK NLENYVCRPA IKTTIKHSRK 

       130        140        150        160        170        180 
ALKSGKMTDE KNEHCPSKWD SSKLFKKAGD ATAIDCQAEE SIHLHSQGES NPLSKKLSPV 

       190        200        210        220        230        240 
HSQMADYISA APSLVGSRDP DIKDRALLNG GTSVTEKLAQ LIATCPPSKS SKAKPKKLGT 

       250        260        270        280        290        300 
GTTVGLVSKD LIRKPGVGSI AGIIHKDLIK KPALSTAVGL VTKDPGKKPM FNAAVGLINK 

       310        320        330        340        350        360 
DSVKKLGTGT TAVFINKDLG KKPGAITTVG LLSKESGKKL GIGIVPGLVN KESGKKLGLG 

       370        380        390        400        410        420 
TVVGLVNKEL GKKLSSTVGL VAKDVTKKIV ASSAMGLVNK DIGKKLLNCP MAGQLGSKDA 

       430        440        450        460        470        480 
LNLKSEALLP TQEQLKASCS ANISNHDSQE LPESLKDSAT GKAFEKSVMR HSKESMLEKF 

       490        500        510        520        530        540 
SVRKEITNLE KEMFNEGTCI QQDNFSSSER GAFETSKHEK QPPVYCTSPD FQIGGASDAS 

       550        560        570        580        590        600 
TAKSPFSAVG ESNLPSSSPT VSVNPVTRSP PEASSQLVPN PLLLNSTAEQ MEEISESIGK 

       610        620        630        640        650        660 
SQFTAESTHL NVGHRSLGHS LSIECKGIDK ELNESKNTHL DIPRISSSLG KKPSLTSDSG 

       670        680        690        700        710        720 
IHAITPSVVN FTSLFSNKPF LKLGAVTAPD KHCQVAESLS SSFQSKPLKK RKGRKPRWTK 

       730        740        750        760        770        780 
VVARSTCRSP KGLDLERSEL FKNVSCSSLS NSSEPAKFMK TIGASSFVDH DFLKRRLPKL 

       790        800        810        820        830        840 
SKSSAPSLAL LTDSEKPSHK SFITHKLSSS MCVTSDLLSD IYKPKRGRPK SKEMPQLEGP 

       850        860        870        880        890        900 
PKRTLKIPAS KVFSLQSKEE QEPPILQPEI EIPSFKQSLS VSPFPKKRGR PKRQMRSPVK 

       910        920        930        940        950        960 
MKPPVLSVAP FVATESPSKL ESESENHRSS SDFFESEDQL QDTDDLDDSH RQSVCSMSDL 

       970        980        990       1000       1010       1020 
EMEPDKKISK RNNGQLMKTI IRKINKMKTL KRKKLLNQIL SSSVESSNKG KVQSKLHNTV 

      1030       1040       1050       1060       1070       1080 
SSLAATFGSK LGQQINVSKK GTIYIGKRRG RKPKTVLNGL LSGSPASLAV LEQTAQQAAG 

      1090       1100       1110       1120       1130       1140 
SALGQILPPL LPSPASSSEI LPSPICSQSS GTSGGQSPVS SDAGFVEPSS VPYLHVHSRQ 

      1150       1160       1170       1180       1190       1200 
GSMIQTLAMK KASKGRRRLS PPTLLPNSPS HLSELTSLKE ATPSPVSESH SDETIPSDSG 

      1210       1220       1230       1240       1250       1260 
IGTDNNSTSD RAEKFCGQKK RRHSFEHISL IPPETSTVLN SLKEKHKHKC KRRSHDYLSY 

      1270       1280       1290       1300       1310       1320 
DKMKRQKRKR KKKYPQLRNR QDPDFIAELE ELISRLSEIR ITHRSHHFIP RDLLPTIFRI 

      1330       1340       1350       1360       1370       1380 
NFNSFYTHPS FPLDPLHYIR KPDLKKKRGR PPKMREAMAE MPFMHSLSFP LSSTGFYPSY 

      1390       1400       1410       1420       1430       1440 
GMPYSPSPLT AAPIGLGYYG RYPPTLYPPP PSPSFTTPLP PPSYMHAGHL LLNPTKYHKK 

      1450       1460       1470       1480       1490       1500 
KHKLLRQEAF LTTSRTPLLS MSTYPSVPPE MAYGWMVEHK HRHRHKHREH RSEQPQVSMD 

      1510       1520       1530       1540       1550       1560 
SGSSRSVLES LKRYRFGKDT VGDRYKHKEK HRCHMSCPHL SPSKNLINRE EQWVSREPSE 

      1570       1580       1590       1600       1610       1620 
SSSLALGLQT PLQIDCSESS PSLSLGGFTP NSEPASSDEH MNLFTSAIGS CRVSNPNSSC 

      1630       1640       1650       1660       1670       1680 
RKKLTDSPGL FPVQDTALNR PHRKEPLPSS ERAIQSLAGS QSASDKPSQR SSESTNCSPT 

      1690       1700       1710       1720       1730       1740 
RKRSSSESTS STVNGVPSRS PRLVASMDDS VDSLLQRIVH HDEQESMEKN GDASITTVSA 

      1750       1760       1770       1780       1790       1800 
PPSSSPGHSY SKERALGKSD SLLVPAVPND SCSNIPLLSE KSASRCSPHH IKRSVVEAMQ 

      1810       1820       1830       1840       1850       1860 
RQARKMCNYD KILATKKNLD HVNKILKAKK LQRQARTGNN FVKRRPGRPR KCPLQAVVSM 

      1870       1880       1890       1900       1910       1920 
QAFQAAQFVS PELNEGEDMS LHLSPDTVTD VIEAVVQSVN LTSEHKKGVK RKNWLLEEQT 

      1930       1940       1950       1960       1970       1980 
RKKQKTVPEE EEQENNKSFI ETPVEIPSPL ETPAEPSEPE NTLQPVLALI PREKKAPRPP 

      1990       2000       2010       2020       2030       2040 
KKKYQRAGLY SDVYKTIDPK SRLIQLKKEK LEYTPGEHEY GLFPAPIHVG KYLRQKRIDF 

      2050       2060       2070       2080       2090       2100 
QLPYDILWQW KHNQLYKKPD VPLYKKIRSN VYVDVKPLSG YEATTCNCKK PDDDTRKGCG 

      2110       2120       2130       2140       2150       2160 
DDCLNRMIFA ECSPNTCPCG EQCCNQRIQR HEWVQCLERF RAEEKGWGIR TKEPLKAGQF 

      2170       2180       2190       2200       2210       2220 
IIEYLGEVVS EQEFRNRMIE QYHNHSDHYC LNLDSGMVID SYRMGNEARF INHSCDPNCE 

      2230       2240       2250       2260       2270       2280 
MQKWSVNGVY RIGLYALKDM PAGTELTYDY NFHSFNVEKQ QLCKCGFEKC RGIIGGKSQR 

      2290       2300       2310       2320       2330       2340 
MNGLPSHKGS QSSSTHRKSA RAKEKRKSKH KLKKRKGHPS EEPSENINTP TRLTPQLQMK 

      2350       2360       2370       2380       2390       2400 
PMSNRERNFV LKHHVFLVRN WEKIHQKQEE VKHTRDIHSA SLYTRWNGLC RDDGNIKSDV 

      2410       2420       2430       2440       2450       2460 
FMTQFSALQT ARSVRTRRLA AAEENLEVAR AARLAQIFKE ICDGIISYRD SSQQTLAAPL 

      2470       2480       2490       2500       2510       2520 
LNLPPKKKNA DYYEKISDPL DLSTIEKQIL IGYYKTVEAF DADMLKVFRN AEKYYGRKSP 

      2530       2540       2550       2560       2570       2580 
IGRDVCRLRK AYYSARHEAS AQIDEIVGET ASEADSSETS VSEKESGHEK DDDVIRCICG 

      2590       2600       2610       2620       2630       2640 
LYKDEGLMIQ CDKCMVWQHC DCMGVNTDVE HYLCEQCDPR PVDREVPMIP RPHYAQPGCV 

      2650       2660       2670       2680       2690       2700 
YFICLLRDDL LLRQGDCVYL MRDSRRTPDG HPVRQSYRLL SHINRDKLDI FRIEKLWKNE 

      2710       2720       2730       2740       2750       2760 
KEERFAFGHH YFRPHETHHS PSRRFYHNEL FRVPLYEIIP LEAVVGTCCV LDLYTYCKGR 

      2770       2780       2790       2800       2810       2820 
PKGIKEQDVY ICDYRLDKSA HLFYKIHRNR YPVCTKPYAF DHFPKKLTPK RDFSPHYVPD 

      2830       2840       2850       2860       2870       2880 
NYKRNGGRSS WKSERSKPPL KDLGQEDDAL PLIEEVLASQ EQAAREVPSP EEPDQERATG 

      2890       2900       2910       2920       2930       2940 
DIGDAEKKPE ESSQEAQLAS TPEERRHSQR ERLNQILLNL LEKIPGKNAI DVTYLLEEGS 

      2950 
GRKLRRRTLF IPENSFRK 

« Hide

References

« Hide 'large scale' references
[1]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[2]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-2005 AND 2048-2465.
Strain: C57BL/6J and NOD.
Tissue: Embryo, Testis and Thymus.
[3]Lubec G., Sunyer B., Chen W.-Q.
Submitted (JAN-2009) to UniProtKB
Cited for: PROTEIN SEQUENCE OF 88-94; 1254-1265; 1730-1752 AND 2270-2277, IDENTIFICATION BY MASS SPECTROMETRY.
Strain: OF1.
Tissue: Hippocampus.
[4]"A novel SWI/SNF family gene in mammals reveals a bromodomain lost in Drosophila ASH1."
Tanaka Y.
Submitted (MAR-2000) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 290-2958.
Tissue: Thymus.
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1996-2958.
Tissue: Limb.
[6]"Label-free quantitative proteomics of the lysine acetylome in mitochondria identifies substrates of SIRT3 in metabolic pathways."
Rardin M.J., Newman J.C., Held J.M., Cusack M.P., Sorensen D.J., Li B., Schilling B., Mooney S.D., Kahn C.R., Verdin E., Gibson B.W.
Proc. Natl. Acad. Sci. U.S.A. 110:6601-6606(2013) [PubMed] [Europe PMC] [Abstract]
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-2307; LYS-2309 AND LYS-2313, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Liver.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AC127377 Genomic DNA. No translation available.
AC140468 Genomic DNA. No translation available.
AK033177 mRNA. Translation: BAC28183.2.
AK034679 mRNA. Translation: BAC28795.2.
AK088497 mRNA. Translation: BAC40390.1.
AK153783 mRNA. Translation: BAE32182.1. Different initiation.
AF247132 mRNA. Translation: AAK26242.1. Different initiation.
BC052194 mRNA. Translation: AAH52194.1.
CCDSCCDS17487.1.
RefSeqNP_619620.3. NM_138679.5.
XP_006501204.1. XM_006501141.1.
XP_006501205.1. XM_006501142.1.
UniGeneMm.130752.

3D structure databases

ProteinModelPortalQ99MY8.
SMRQ99MY8. Positions 2064-2275, 2425-2550.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid228672. 2 interactions.

PTM databases

PhosphoSiteQ99MY8.

Proteomic databases

PaxDbQ99MY8.
PRIDEQ99MY8.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000090933; ENSMUSP00000088451; ENSMUSG00000028053.
GeneID192195.
KEGGmmu:192195.
UCSCuc008pxi.1. mouse.

Organism-specific databases

CTD55870.
MGIMGI:2183158. Ash1l.

Phylogenomic databases

eggNOGCOG2940.
GeneTreeENSGT00740000114921.
HOVERGENHBG080871.
InParanoidQ99MY8.
KOK06101.
OMAPENSFRK.
OrthoDBEOG7ZPNJ4.
TreeFamTF106416.

Gene expression databases

BgeeQ99MY8.
GenevestigatorQ99MY8.

Family and domain databases

Gene3D1.20.920.10. 1 hit.
3.30.40.10. 1 hit.
InterProIPR017956. AT_hook_DNA-bd_motif.
IPR006560. AWS.
IPR001025. BAH_dom.
IPR001487. Bromodomain.
IPR003616. Post-SET_dom.
IPR001214. SET_dom.
IPR019786. Zinc_finger_PHD-type_CS.
IPR011011. Znf_FYVE_PHD.
IPR001965. Znf_PHD.
IPR019787. Znf_PHD-finger.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PfamPF01426. BAH. 1 hit.
PF00439. Bromodomain. 1 hit.
PF00628. PHD. 1 hit.
PF00856. SET. 1 hit.
[Graphical view]
SMARTSM00384. AT_hook. 4 hits.
SM00570. AWS. 1 hit.
SM00439. BAH. 1 hit.
SM00297. BROMO. 1 hit.
SM00249. PHD. 1 hit.
SM00508. PostSET. 1 hit.
SM00317. SET. 1 hit.
[Graphical view]
SUPFAMSSF47370. SSF47370. 1 hit.
SSF57903. SSF57903. 1 hit.
PROSITEPS51215. AWS. 1 hit.
PS51038. BAH. 1 hit.
PS50014. BROMODOMAIN_2. 1 hit.
PS50868. POST_SET. 1 hit.
PS50280. SET. 1 hit.
PS01359. ZF_PHD_1. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSASH1L. mouse.
NextBio371226.
PROQ99MY8.
SOURCESearch...

Entry information

Entry nameASH1L_MOUSE
AccessionPrimary (citable) accession number: Q99MY8
Secondary accession number(s): E9QNM2 expand/collapse secondary AC list , Q3U598, Q80VY5, Q8BM69, Q8BTX0, Q8BZY6
Entry history
Integrated into UniProtKB/Swiss-Prot: October 31, 2006
Last sequence update: July 27, 2011
Last modified: July 9, 2014
This is version 101 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot