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Protein

Diphosphoinositol polyphosphate phosphohydrolase 2

Gene

Nudt4

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Cleaves a beta-phosphate from the diphosphate groups in PP-InsP5 (diphosphoinositol pentakisphosphate), PP-InsP4 and [PP]2-InsP4 (bisdiphosphoinositol tetrakisphosphate), suggesting that it may play a role in signal transduction. Also able to catalyze the hydrolysis of dinucleoside oligophosphate Ap6A, but not Ap5A. The major reaction products are ADP and p4a from Ap6A. Also able to hydrolyze 5-phosphoribose 1-diphosphate. Does not play a role in U8 snoRNA decapping activity. Binds U8 snoRNA (By similarity).By similarity

Catalytic activityi

Diphospho-myo-inositol polyphosphate + H2O = myo-inositol polyphosphate + phosphate.

Cofactori

Mg2+By similarity, Mn2+By similarityNote: Binds 3 Mg2+ or Mn2+ ions per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei9 – 91SubstrateBy similarity
Binding sitei40 – 401SubstrateBy similarity
Metal bindingi49 – 491Magnesium 1; via carbonyl oxygenBy similarity
Metal bindingi65 – 651Magnesium 2By similarity
Metal bindingi65 – 651Magnesium 3By similarity
Active sitei68 – 681Proton acceptorBy similarity
Metal bindingi69 – 691Magnesium 1By similarity

GO - Molecular functioni

GO - Biological processi

  • phosphatidylinositol metabolic process Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Magnesium, Manganese, Metal-binding, RNA-binding

Enzyme and pathway databases

ReactomeiR-RNO-1855167. Synthesis of pyrophosphates in the cytosol.

Names & Taxonomyi

Protein namesi
Recommended name:
Diphosphoinositol polyphosphate phosphohydrolase 2 (EC:3.6.1.52)
Short name:
DIPP-2
Short name:
rDIPP2
Alternative name(s):
Diadenosine 5',5'''-P1,P6-hexaphosphate hydrolase 2 (EC:3.6.1.-)
Nucleoside diphosphate-linked moiety X motif 4
Short name:
Nudix motif 4
Gene namesi
Name:Nudt4
Synonyms:Dipp2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 7

Organism-specific databases

RGDi621355. Nudt4.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 179179Diphosphoinositol polyphosphate phosphohydrolase 2PRO_0000057061Add
BLAST

Proteomic databases

PaxDbiQ99MY2.
PRIDEiQ99MY2.

PTM databases

iPTMnetiQ99MY2.
PhosphoSiteiQ99MY2.

Expressioni

Inductioni

Overexpressed in frontal cortex upon chronic lithium treatment.1 Publication

Gene expression databases

GenevisibleiQ99MY2. RN.

Interactioni

Protein-protein interaction databases

IntActiQ99MY2. 1 interaction.
STRINGi10116.ENSRNOP00000012363.

Structurei

3D structure databases

ProteinModelPortaliQ99MY2.
SMRiQ99MY2. Positions 8-145.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini17 – 143127Nudix hydrolasePROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni17 – 193Substrate bindingBy similarity
Regioni88 – 903Substrate bindingBy similarity

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi50 – 7122Nudix boxAdd
BLAST

Sequence similaritiesi

Belongs to the Nudix hydrolase family. DIPP subfamily.Curated
Contains 1 nudix hydrolase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG2839. Eukaryota.
COG0494. LUCA.
GeneTreeiENSGT00390000012928.
HOGENOMiHOG000237336.
HOVERGENiHBG053341.
InParanoidiQ99MY2.
KOiK07766.
OMAiENSSWIV.
OrthoDBiEOG7T7GVQ.
PhylomeDBiQ99MY2.
TreeFamiTF106349.

Family and domain databases

Gene3Di3.90.79.10. 1 hit.
InterProiIPR020084. NUDIX_hydrolase_CS.
IPR000086. NUDIX_hydrolase_dom.
IPR015797. NUDIX_hydrolase_dom-like.
[Graphical view]
PfamiPF00293. NUDIX. 1 hit.
[Graphical view]
SUPFAMiSSF55811. SSF55811. 1 hit.
PROSITEiPS51462. NUDIX. 1 hit.
PS00893. NUDIX_BOX. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q99MY2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKFKPNQTRT YDREGFKKRA ACLCFRSEQE DEVLLVSSSR YPDQWIVPGG
60 70 80 90 100
GVEPEEEPGG AAAREVYEEA GVKGKLGRLL GIFENQDRKH RTYVYVLTVT
110 120 130 140 150
EILEDWEDSV NIGRKREWFK VEDAIKVLQC HKPVHAEYLE RLKLGCSPTN
160 170
GNSTVPSLPD NNALFVTAAP PSGVPSSIR
Length:179
Mass (Da):20,138
Last modified:June 1, 2001 - v1
Checksum:iDE59B9CCA615A6A9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF253473 mRNA. Translation: AAK29279.1.
RefSeqiNP_446050.1. NM_053598.1.
UniGeneiRn.8426.

Genome annotation databases

EnsembliENSRNOT00000012363; ENSRNOP00000012363; ENSRNOG00000009094.
GeneIDi94267.
KEGGirno:94267.
UCSCiRGD:621355. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF253473 mRNA. Translation: AAK29279.1.
RefSeqiNP_446050.1. NM_053598.1.
UniGeneiRn.8426.

3D structure databases

ProteinModelPortaliQ99MY2.
SMRiQ99MY2. Positions 8-145.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ99MY2. 1 interaction.
STRINGi10116.ENSRNOP00000012363.

PTM databases

iPTMnetiQ99MY2.
PhosphoSiteiQ99MY2.

Proteomic databases

PaxDbiQ99MY2.
PRIDEiQ99MY2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000012363; ENSRNOP00000012363; ENSRNOG00000009094.
GeneIDi94267.
KEGGirno:94267.
UCSCiRGD:621355. rat.

Organism-specific databases

CTDi11163.
RGDi621355. Nudt4.

Phylogenomic databases

eggNOGiKOG2839. Eukaryota.
COG0494. LUCA.
GeneTreeiENSGT00390000012928.
HOGENOMiHOG000237336.
HOVERGENiHBG053341.
InParanoidiQ99MY2.
KOiK07766.
OMAiENSSWIV.
OrthoDBiEOG7T7GVQ.
PhylomeDBiQ99MY2.
TreeFamiTF106349.

Enzyme and pathway databases

ReactomeiR-RNO-1855167. Synthesis of pyrophosphates in the cytosol.

Miscellaneous databases

PROiQ99MY2.

Gene expression databases

GenevisibleiQ99MY2. RN.

Family and domain databases

Gene3Di3.90.79.10. 1 hit.
InterProiIPR020084. NUDIX_hydrolase_CS.
IPR000086. NUDIX_hydrolase_dom.
IPR015797. NUDIX_hydrolase_dom-like.
[Graphical view]
PfamiPF00293. NUDIX. 1 hit.
[Graphical view]
SUPFAMiSSF55811. SSF55811. 1 hit.
PROSITEiPS51462. NUDIX. 1 hit.
PS00893. NUDIX_BOX. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Molecular cloning of a novel isoform of diphosphoinositol polyphosphate phosphohydrolase: a potential target of lithium therapy."
    Hua L.V., Green M., Warsh J.J., Li P.P.
    Neuropsychopharmacology 24:640-651(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], INDUCTION.
    Strain: Sprague-Dawley.

Entry informationi

Entry nameiNUDT4_RAT
AccessioniPrimary (citable) accession number: Q99MY2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 5, 2005
Last sequence update: June 1, 2001
Last modified: June 8, 2016
This is version 103 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.