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Protein

Spermatogenic leucine zipper protein 1

Gene

Spz1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transcription factor that binds to the DNA sequence 5'-CANNTG-3'(E box) and the G-box motif. Directly binds to a guanine-rich region of the PCNA promoter and up-regulates its expression which in turn induces cell transformation and tumor formation. May play an important role in the regulation of cell proliferation and differentiation during spermatogenesis.3 Publications

GO - Molecular functioni

  • DNA binding Source: UniProtKB-KW
  • transcription factor activity, sequence-specific DNA binding Source: MGI

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Spermatogenic leucine zipper protein 1
Alternative name(s):
BHLH-Zip transcription factor SPZ1
Spermatogenic Zip 1
Gene namesi
Name:Spz1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 13

Organism-specific databases

MGIiMGI:1930801. Spz1.

Subcellular locationi

  • Cytoplasm 1 Publication
  • Nucleus 2 Publications

GO - Cellular componenti

  • cytoplasm Source: UniProtKB-SubCell
  • nucleus Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Keywords - Diseasei

Proto-oncogene

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 378378Spermatogenic leucine zipper protein 1PRO_0000280508Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei106 – 1061PhosphoserineBy similarity
Modified residuei207 – 2071PhosphoserineBy similarity

Post-translational modificationi

Phosphorylated by MAPK1/ERK2 and MAPK3/ERK1.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ99MY0.
PRIDEiQ99MY0.

PTM databases

PhosphoSiteiQ99MY0.

Expressioni

Tissue specificityi

Expressed specifically in the testis and epidydimis. In the testis expressed in both germ cells and somatic cells (Sertoli and Leydig cells). Expressed in several tumor cell lines.3 Publications

Developmental stagei

First detected in the testis at postnatal day 10 and levels increase further and reach highest levels at day 40 and then gradually decrease.1 Publication

Inductioni

Down-regulated by testosterone and retinoic acid.1 Publication

Gene expression databases

BgeeiQ99MY0.
CleanExiMM_SPZ1.
GenevisibleiQ99MY0. MM.

Interactioni

Subunit structurei

Interacts with PPP1CC isoform gamma-2. This interaction can prevent SPZ1 binding to the E-box and inhibits PPP1CC activity.1 Publication

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000054083.

Structurei

3D structure databases

ProteinModelPortaliQ99MY0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni116 – 12712Helix-loop-helix motifSequence analysisAdd
BLAST
Regioni116 – 1227Interaction with PPP1CC isoform gamma-2
Regioni128 – 19366Basic motifSequence analysisAdd
BLAST
Regioni252 – 27322Leucine-zipperAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili182 – 23352Sequence analysisAdd
BLAST

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiENOG410IVK6. Eukaryota.
ENOG411141P. LUCA.
GeneTreeiENSGT00390000003379.
HOGENOMiHOG000154405.
HOVERGENiHBG093995.
InParanoidiQ99MY0.
KOiK17482.
OMAiLENECQI.
OrthoDBiEOG74TWXQ.
PhylomeDBiQ99MY0.
TreeFamiTF337798.

Family and domain databases

InterProiIPR029780. SPZ1.
[Graphical view]
PANTHERiPTHR23158:SF40. PTHR23158:SF40. 1 hit.

Sequencei

Sequence statusi: Complete.

Q99MY0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSDTDNSAEM PARCPSPNPA PGAKQEPPNS GITISLLEIG SLPTVCYHSF
60 70 80 90 100
PPPKNSICPV EKRGRVQKFS NLLKDVKDVL KNIAGVEEKS TVGEPFDDAY
110 120 130 140 150
IPEDLSELNV RGVEKKNKIR FKDDLFIHFD PEREQNTMKQ MLLKNQSAKN
160 170 180 190 200
MVPKFARDLC NAEETRGFDG MLLSVKRPRN GSLHLRGEYR KLRNNMEQLL
210 220 230 240 250
QEADHWSKQH NELSELMRSY QECQNETQET TDKDRACLQN QPNNGLSTKQ
260 270 280 290 300
KLEEQVKKLS HDTHALHLIA ALLENECQVL QQRVDILKDF HLHEAGLGHE
310 320 330 340 350
KPLQMSCEQD KKCPKLAEAD KTDAFKHTTR ATEGTIRKPK ILRSPDVCFT
360 370
KKARNNRFNA RVAKKSLVGK RRTVSSFR
Length:378
Mass (Da):43,091
Last modified:June 1, 2001 - v1
Checksum:iEB83F5A921046672
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti261 – 2611H → Q in BAE43195 (PubMed:16141072).Curated
Sequence conflicti297 – 2971L → P in AAH50748 (PubMed:15489334).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF271697 mRNA. Translation: AAK15458.1.
AK006437 mRNA. Translation: BAE43195.1.
BC050748 mRNA. Translation: AAH50748.1.
CCDSiCCDS26683.1.
RefSeqiNP_084513.3. NM_030237.3.
UniGeneiMm.23520.

Genome annotation databases

EnsembliENSMUST00000050658; ENSMUSP00000054083; ENSMUSG00000046957.
GeneIDi79401.
KEGGimmu:79401.
UCSCiuc007rkr.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF271697 mRNA. Translation: AAK15458.1.
AK006437 mRNA. Translation: BAE43195.1.
BC050748 mRNA. Translation: AAH50748.1.
CCDSiCCDS26683.1.
RefSeqiNP_084513.3. NM_030237.3.
UniGeneiMm.23520.

3D structure databases

ProteinModelPortaliQ99MY0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000054083.

PTM databases

PhosphoSiteiQ99MY0.

Proteomic databases

PaxDbiQ99MY0.
PRIDEiQ99MY0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000050658; ENSMUSP00000054083; ENSMUSG00000046957.
GeneIDi79401.
KEGGimmu:79401.
UCSCiuc007rkr.2. mouse.

Organism-specific databases

CTDi84654.
MGIiMGI:1930801. Spz1.

Phylogenomic databases

eggNOGiENOG410IVK6. Eukaryota.
ENOG411141P. LUCA.
GeneTreeiENSGT00390000003379.
HOGENOMiHOG000154405.
HOVERGENiHBG093995.
InParanoidiQ99MY0.
KOiK17482.
OMAiLENECQI.
OrthoDBiEOG74TWXQ.
PhylomeDBiQ99MY0.
TreeFamiTF337798.

Miscellaneous databases

NextBioi349915.
PROiQ99MY0.
SOURCEiSearch...

Gene expression databases

BgeeiQ99MY0.
CleanExiMM_SPZ1.
GenevisibleiQ99MY0. MM.

Family and domain databases

InterProiIPR029780. SPZ1.
[Graphical view]
PANTHERiPTHR23158:SF40. PTHR23158:SF40. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Spz1, a novel bHLH-Zip protein, is specifically expressed in testis."
    Hsu S.-H., Shyu H.-W., Hsieh-Li H.-M., Li H.
    Mech. Dev. 100:177-187(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, INDUCTION, DEVELOPMENTAL STAGE.
    Strain: C57BL/6J.
    Tissue: Testis.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Testis.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Testis.
  4. "Identification of the spermatogenic zip protein Spz1 as a putative protein phosphatase-1 (PP1) regulatory protein that specifically binds the PP1cgamma2 splice variant in mouse testis."
    Hrabchak C., Varmuza S.
    J. Biol. Chem. 279:37079-37086(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, INTERACTION WITH PPP1CC ISOFORM GAMMA-2.
  5. "Dysfunctional spermatogenesis in transgenic mice overexpressing bHLH-Zip transcription factor, Spz1."
    Hsu S.-H., Hsieh-Li H.-M., Li H.
    Exp. Cell Res. 294:185-198(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TISSUE SPECIFICITY.
  6. "bHLH-zip transcription factor Spz1 mediates mitogen-activated protein kinase cell proliferation, transformation, and tumorigenesis."
    Hsu S.-H., Hsieh-Li H.-M., Huang H.-Y., Huang P.-H., Li H.
    Cancer Res. 65:4041-4050(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TISSUE SPECIFICITY, PHOSPHORYLATION.

Entry informationi

Entry nameiSPZ1_MOUSE
AccessioniPrimary (citable) accession number: Q99MY0
Secondary accession number(s): Q3V487, Q80X69
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 20, 2007
Last sequence update: June 1, 2001
Last modified: January 20, 2016
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

The helix-loop-helix and basic motifs form a Spz1 specific bHLH different from the classical one.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.