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Protein

RING finger protein 17

Gene

Rnf17

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Seems to be involved in regulation of transcriptional activity of MYC. In vitro, inhibits DNA-binding activity of Mad-MAX heterodimers. Can recruit Mad transcriptional repressors (MXD1, MXD3, MXD4 and MXI1) to the cytoplasm. May be involved in spermiogenesis.2 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri30 – 73RING-typePROSITE-ProRule annotationAdd BLAST44

GO - Molecular functioni

  • protein homodimerization activity Source: MGI
  • zinc ion binding Source: InterPro

GO - Biological processi

  • multicellular organism development Source: UniProtKB-KW
  • spermatid development Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Biological processi

Differentiation, Spermatogenesis

Keywords - Ligandi

Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
RING finger protein 17
Alternative name(s):
Mad member-interacting protein 2
Short name:
Mmip-2
Gene namesi
Name:Rnf17
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 14

Organism-specific databases

MGIiMGI:1353419. Rnf17.

Subcellular locationi

  • Cytoplasm
  • Nucleus

  • Note: Predominantly found in the cytoplasm. Component of a nuage in male germ cells (an electron-dense spherical cytoplasmic body present in late pachytene and diplotene spermatocytes and in elonging spermatids).

GO - Cellular componenti

  • cytoplasm Source: MGI
  • nucleus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Disruption phenotypei

Male mice are sterile, exhibit a complete arrest in round spermatids and fail to produce sperm.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000560601 – 1640RING finger protein 17Add BLAST1640

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei134PhosphoserineCombined sources1
Modified residuei229N6-acetyllysineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ99MV7.
PeptideAtlasiQ99MV7.
PRIDEiQ99MV7.

PTM databases

iPTMnetiQ99MV7.
PhosphoSitePlusiQ99MV7.

Expressioni

Tissue specificityi

Expressed at high levels in adult testis. Expressed in male germ cells (at protein level). Expressed at lower levels in adult thyroid, submaxillary gland, ovary and epididymis.2 Publications

Gene expression databases

BgeeiENSMUSG00000000365.
CleanExiMM_RNF17.
GenevisibleiQ99MV7. MM.

Interactioni

Subunit structurei

Interacts with MXD1, MXD3, MXD4, MXI1 and PIWIL1. Self-associates.2 Publications

GO - Molecular functioni

  • protein homodimerization activity Source: MGI

Protein-protein interaction databases

BioGridi205956. 1 interactor.
IntActiQ99MV7. 2 interactors.
MINTiMINT-8174120.
STRINGi10090.ENSMUSP00000093469.

Structurei

3D structure databases

ProteinModelPortaliQ99MV7.
SMRiQ99MV7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini751 – 809Tudor 1PROSITE-ProRule annotationAdd BLAST59
Domaini985 – 1044Tudor 2PROSITE-ProRule annotationAdd BLAST60
Domaini1246 – 1303Tudor 3PROSITE-ProRule annotationAdd BLAST58
Domaini1496 – 1556Tudor 4PROSITE-ProRule annotationAdd BLAST61

Sequence similaritiesi

Contains 1 RING-type zinc finger.PROSITE-ProRule annotation
Contains 4 Tudor domains.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri30 – 73RING-typePROSITE-ProRule annotationAdd BLAST44

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiENOG410IPQY. Eukaryota.
ENOG410ZWK2. LUCA.
GeneTreeiENSGT00730000110295.
HOGENOMiHOG000074158.
HOVERGENiHBG108414.
InParanoidiQ99MV7.
KOiK18405.
OMAiFEDGVWY.
OrthoDBiEOG091G00SE.
PhylomeDBiQ99MV7.

Family and domain databases

Gene3Di2.40.50.90. 1 hit.
InterProiIPR016071. Staphylococal_nuclease_OB-fold.
IPR002999. Tudor.
IPR001841. Znf_RING.
IPR017907. Znf_RING_CS.
[Graphical view]
PfamiPF00567. TUDOR. 5 hits.
[Graphical view]
SMARTiSM00333. TUDOR. 4 hits.
[Graphical view]
SUPFAMiSSF50199. SSF50199. 1 hit.
PROSITEiPS50304. TUDOR. 4 hits.
PS00518. ZF_RING_1. 1 hit.
PS50089. ZF_RING_2. 1 hit.
[Graphical view]

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q99MV7-1) [UniParc]FASTAAdd to basket
Also known as: RNF17L

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAAEASSTGL ASCHLVESKS GAQGASGCQC TRCGRKVSVA SGDHHKFPCG
60 70 80 90 100
HAFCELCLLA PQEYTTSKCT DCEVHTTVSM NQGHYPVDGF IEEDSSLEAL
110 120 130 140 150
PPKMVNNCSS DLEKTVDQLI NDLEHSSSIH RNVSNPSAVM SETEEIDEAL
160 170 180 190 200
KIAGCNFEQL SNAIKMLDST QDQTRQETHS LTEAVEKQFD TLLASLDSRK
210 220 230 240 250
KSLCEELIRR TDDYLSKLVT VKSYIEEKKS DLDAAMKIAK ELRSAPSLRT
260 270 280 290 300
YCDLTQIIRT LKLTFESELS QVSSIIPRNT PRLDINCSEA ICMFSSMGKI
310 320 330 340 350
EFEDSTKCYP QENEDGQNVQ KKFNNRKELC CDVYSSLEKK KVDAAVLTDE
360 370 380 390 400
TPEPPLQAEA PDRHLEGKKK QPTKEMVVVT SPKTIAVLPQ LGSSPDVIIE
410 420 430 440 450
EIIEENLESC FTDDPIETSG YPKKPPQKEQ SAPVGSKAGC PELVFVSHVI
460 470 480 490 500
HPCHFYVRKY SQIKDATILE KKMKQVCNRS LHLDPSDILE LGARIFVNSI
510 520 530 540 550
KNRMWCRGII TEIIPSKTKN IRKPCSPTKF SVCEISLIQI FMVDFGNSEV
560 570 580 590 600
LIITGVGDTH EGPEHDGEQH ITLSDFCLLL MKSEPYSEEL LKDIPHLAHL
610 620 630 640 650
CSLKDIVPYN STEGWEKEAK VEFLKMVNKK AVLMKVFGEE DDVLIVDLQK
660 670 680 690 700
PPTNKISSDM PVSLRDALVF MELARFRSQS PRSHSEKNTT LCYHPPILPE
710 720 730 740 750
EMTEVSVMVC HINSPTDFYL QLMENLDFLS LLKTIEEFYK GEDGENLEIL
760 770 780 790 800
CPLQNQACVA KFEDGIWYRA KVIGLPGHRE VEVKYVDFGN TAKITLKDMR
810 820 830 840 850
KIKDEFLEPP EKAIKCKLAY VEPSKKSQWS KKAKEKFEEK TQDKFVTCSV
860 870 880 890 900
IKILENNVLL VELFDSRAPG KSAVSINDQL VKEGLASYEA GYTLKDNSKK
910 920 930 940 950
HLEVWDPSPE EIITSEINNL SPLSVKSLPN ENFQSLYNKE LPVNICNVIS
960 970 980 990 1000
PEKIYVQWLL TENLLNSLEE KMVAAYEHSE WKPVKWECDM HCAVKVPAKN
1010 1020 1030 1040 1050
QWRRGQILRM VTDKLVEVLL YDVGVELVVN IHCLRELQEN LKTMGRLSLE
1060 1070 1080 1090 1100
CSLVDIRPTG GSDKWTATAC DCLSLHLTGA IATIILQESN TTWPLPVKIF
1110 1120 1130 1140 1150
CRDEKGERVD VSKYLIKKGL ALRERRVSKS SNSHSPEKSL EIPLEQGDSV
1160 1170 1180 1190 1200
VTKCFKINFD TNKKIADKVN EHKVPDSKGK KSESRSTGCY RPPAVPNTSS
1210 1220 1230 1240 1250
FEAIVTCIGD DGTIFVVPKL SEFELIKMMD EIQSNLKCLG LLEPYSWKKG
1260 1270 1280 1290 1300
EPCAVRGSDT LWYRGKVMEV VGGTIRVQYL DHGFTEKIPQ CHLYPILLYP
1310 1320 1330 1340 1350
DTPQFCIPCQ LYQTLPVGNT WQPDAIELLQ ELLSKREVDI HIMELPNNSW
1360 1370 1380 1390 1400
GKLSVHLYFD GMSLSHFMAH HKYCIFEHTE EIFKEKPRGQ NKKYEDENWK
1410 1420 1430 1440 1450
IRFEDLLLPE MEAPVLPPYL SSLLPPPEEL FAVQVKHIVS PDEMYICLDS
1460 1470 1480 1490 1500
EDSYTQFNHH GDTDDSGVSW ESESENLEEA LQRFNKNVET FPPLTDFSSE
1510 1520 1530 1540 1550
MPCLAEYADG LWYRAKIISI KEFNPLSVLV LFVDYGCTEK LTINRLRQIP
1560 1570 1580 1590 1600
VQLMQYPAQA IKVLLAGFKP PLSDSGKTRI PYCPKWSMEA LWTMIDCLQG
1610 1620 1630 1640
KQLYASSVAQ APEQIVTLYE DEQYPVHMSL VEMGLADKDE
Length:1,640
Mass (Da):185,600
Last modified:April 29, 2008 - v2
Checksum:iF6597E5750AE5ECC
GO
Isoform 2 (identifier: Q99MV7-2) [UniParc]FASTAAdd to basket
Also known as: RNF17S

The sequence of this isoform differs from the canonical sequence as follows:
     1127-1129: VSK → ASS
     1130-1640: Missing.

Note: Major.
Show »
Length:1,129
Mass (Da):127,176
Checksum:iB7E1C7B5983E2CF0
GO
Isoform 3 (identifier: Q99MV7-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-580: Missing.

Show »
Length:1,060
Mass (Da):121,166
Checksum:i62038B1F91262AD1
GO
Isoform 4 (identifier: Q99MV7-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     612-626: TEGWEKEAKVEFLKM → VSERESDSPSKAVEF
     627-1640: Missing.

Show »
Length:626
Mass (Da):69,653
Checksum:iAC8084E425727349
GO

Sequence cautioni

The sequence AAF01336 differs from that shown. Reason: Frameshift at positions 311 and 315.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti20S → SGAQ in AAF01336 (PubMed:10597267).Curated1
Sequence conflicti23Q → S in AAF01336 (PubMed:10597267).Curated1
Sequence conflicti282R → E in AAF01336 (PubMed:10597267).Curated1
Sequence conflicti307K → P in AAF01336 (PubMed:10597267).Curated1
Sequence conflicti311Q → L in AAF01336 (PubMed:10597267).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0330771 – 580Missing in isoform 3. 1 PublicationAdd BLAST580
Alternative sequenceiVSP_033078612 – 626TEGWE…EFLKM → VSERESDSPSKAVEF in isoform 4. 1 PublicationAdd BLAST15
Alternative sequenceiVSP_033079627 – 1640Missing in isoform 4. 1 PublicationAdd BLAST1014
Alternative sequenceiVSP_0330801127 – 1129VSK → ASS in isoform 2. 2 Publications3
Alternative sequenceiVSP_0330811130 – 1640Missing in isoform 2. 2 PublicationsAdd BLAST511

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF285585 mRNA. Translation: AAK31964.1.
AY854010 mRNA. Translation: AAX51690.1.
AY854011 mRNA. Translation: AAX51691.1.
AF190166 mRNA. Translation: AAF01336.1. Frameshift.
AK029693 mRNA. Translation: BAC26567.1.
BC099501 mRNA. Translation: AAH99501.1.
CCDSiCCDS27149.1. [Q99MV7-1]
RefSeqiNP_001028215.1. NM_001033043.1. [Q99MV7-1]
XP_006519173.1. XM_006519110.3.
XP_006519175.1. XM_006519112.1. [Q99MV7-3]
XP_017171542.1. XM_017316053.1. [Q99MV7-1]
UniGeneiMm.287783.

Genome annotation databases

EnsembliENSMUST00000095793; ENSMUSP00000093469; ENSMUSG00000000365. [Q99MV7-1]
GeneIDi30054.
KEGGimmu:30054.
UCSCiuc007ubx.1. mouse. [Q99MV7-4]
uc007ubz.1. mouse. [Q99MV7-2]
uc007uca.1. mouse. [Q99MV7-1]
uc011zma.1. mouse. [Q99MV7-3]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF285585 mRNA. Translation: AAK31964.1.
AY854010 mRNA. Translation: AAX51690.1.
AY854011 mRNA. Translation: AAX51691.1.
AF190166 mRNA. Translation: AAF01336.1. Frameshift.
AK029693 mRNA. Translation: BAC26567.1.
BC099501 mRNA. Translation: AAH99501.1.
CCDSiCCDS27149.1. [Q99MV7-1]
RefSeqiNP_001028215.1. NM_001033043.1. [Q99MV7-1]
XP_006519173.1. XM_006519110.3.
XP_006519175.1. XM_006519112.1. [Q99MV7-3]
XP_017171542.1. XM_017316053.1. [Q99MV7-1]
UniGeneiMm.287783.

3D structure databases

ProteinModelPortaliQ99MV7.
SMRiQ99MV7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi205956. 1 interactor.
IntActiQ99MV7. 2 interactors.
MINTiMINT-8174120.
STRINGi10090.ENSMUSP00000093469.

PTM databases

iPTMnetiQ99MV7.
PhosphoSitePlusiQ99MV7.

Proteomic databases

PaxDbiQ99MV7.
PeptideAtlasiQ99MV7.
PRIDEiQ99MV7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000095793; ENSMUSP00000093469; ENSMUSG00000000365. [Q99MV7-1]
GeneIDi30054.
KEGGimmu:30054.
UCSCiuc007ubx.1. mouse. [Q99MV7-4]
uc007ubz.1. mouse. [Q99MV7-2]
uc007uca.1. mouse. [Q99MV7-1]
uc011zma.1. mouse. [Q99MV7-3]

Organism-specific databases

CTDi56163.
MGIiMGI:1353419. Rnf17.

Phylogenomic databases

eggNOGiENOG410IPQY. Eukaryota.
ENOG410ZWK2. LUCA.
GeneTreeiENSGT00730000110295.
HOGENOMiHOG000074158.
HOVERGENiHBG108414.
InParanoidiQ99MV7.
KOiK18405.
OMAiFEDGVWY.
OrthoDBiEOG091G00SE.
PhylomeDBiQ99MV7.

Miscellaneous databases

PROiQ99MV7.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000000365.
CleanExiMM_RNF17.
GenevisibleiQ99MV7. MM.

Family and domain databases

Gene3Di2.40.50.90. 1 hit.
InterProiIPR016071. Staphylococal_nuclease_OB-fold.
IPR002999. Tudor.
IPR001841. Znf_RING.
IPR017907. Znf_RING_CS.
[Graphical view]
PfamiPF00567. TUDOR. 5 hits.
[Graphical view]
SMARTiSM00333. TUDOR. 4 hits.
[Graphical view]
SUPFAMiSSF50199. SSF50199. 1 hit.
PROSITEiPS50304. TUDOR. 4 hits.
PS00518. ZF_RING_1. 1 hit.
PS50089. ZF_RING_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRNF17_MOUSE
AccessioniPrimary (citable) accession number: Q99MV7
Secondary accession number(s): Q3T4H2
, Q3T4H3, Q4FZG6, Q8CDR4, Q9QXQ2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 24, 2001
Last sequence update: April 29, 2008
Last modified: November 2, 2016
This is version 119 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.