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Protein

RNA helicase Mov10l1

Gene

Mov10l1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Isoform 1: ATP-dependent RNA helicase required during spermatogenesis to repress transposable elements and prevent their mobilization, which is essential for germline integrity (PubMed:20534472, PubMed:20547853, PubMed:23166510, PubMed:25762440). Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and governs the methylation and subsequent repression of transposons (PubMed:20534472, PubMed:20547853, PubMed:23166510, PubMed:25762440). Involved in the primary piRNA metabolic process (PubMed:20534472, PubMed:20547853, PubMed:23166510, PubMed:25762440). Specifically binds to piRNA precursors and promotes the generation of intermediate piRNA processing fragments that are subsequently loaded to Piwi proteins (PubMed:25762440). Acts via its ATP-dependent RNA helicase activity: displays 5'-3' RNA unwinding activity and probably mediates unwinding and funneling of single-stranded piRNA precursor transcripts to the endonuclease that catalyzes the first cleavage step of piRNA processing to generate piRNA intermediate fragments that are subsequently loaded to Piwi proteins (PubMed:25762440).4 Publications
Isoform 2: May act downstream of MEF2C during heart formation. Acts as a cardiac-specific suppressor of cardiomyocyte hypertrophy and cell cycle progression, suggesting that it may suppress these processes through the regulation of CDKN1A. Such results however require additional evidence.1 Publication

Catalytic activityi

ATP + H2O = ADP + phosphate.1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi772 – 7798ATPSequence analysis

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • ATP-dependent RNA helicase activity Source: UniProtKB
  • piRNA binding Source: UniProtKB

GO - Biological processi

  • cell differentiation Source: UniProtKB-KW
  • cell proliferation Source: UniProtKB
  • DNA methylation involved in gamete generation Source: UniProtKB
  • gene silencing by RNA Source: UniProtKB-KW
  • male meiosis Source: UniProtKB
  • male meiosis I Source: UniProtKB
  • muscle organ development Source: UniProtKB
  • negative regulation of cell cycle Source: UniProtKB
  • piRNA metabolic process Source: UniProtKB
  • spermatogenesis Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Helicase, Hydrolase

Keywords - Biological processi

Differentiation, Meiosis, RNA-mediated gene silencing, Spermatogenesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding, RNA-binding

Enzyme and pathway databases

ReactomeiR-MMU-5601884. PIWI-interacting RNA (piRNA) biogenesis.

Names & Taxonomyi

Protein namesi
Recommended name:
RNA helicase Mov10l1Curated (EC:3.6.4.131 Publication)
Alternative name(s):
Cardiac helicase activated by MEF2 protein1 Publication
Cardiac-specific RNA helicase1 Publication
Moloney leukemia virus 10-like protein 1 homologCurated
Short name:
MOV10-like protein 1 homologCurated
Gene namesi
Name:Mov10l1Imported
Synonyms:Champ1 Publication
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:1891384. Mov10l1.

Subcellular locationi

Isoform 1 :
Isoform 2 :
  • Cytoplasm 1 Publication

GO - Cellular componenti

  • cytosol Source: Reactome
  • P granule Source: UniProtKB
  • pi-body Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

Mice are viable and healthy but show male sterility due to defects in spermatogenesis at early prophase of meiosis I (PubMed:20534472, PubMed:20547853, PubMed:23166510). Retrotransposons are derepressed due to DNA demethylation (PubMed:20534472). Defects are caused by impaired piRNA biogenesis during pachytene (PubMed:20534472, PubMed:20547853). The absence of pachytene piRNAs causes disruption of germ cell development and results in defects in post-meiotic genome integrity (PubMed:23166510). Mice do not show any cardiac abnormalities (PubMed:20547853).3 Publications

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi778 – 7781K → A: Catalytically inactive mutant. Homozygous knockin male mice are sterile and display meiotic arrest at the zygotene-like stage of prophase I. LINE1 retrotransposons are derepressed and piRNA biogenesis is abolished. 1 Publication
Mutagenesisi888 – 8925DEAGQ → GGAAG: Isoform 2: Abolishes the suppressor of cell proliferation activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 11871187RNA helicase Mov10l1PRO_0000080707Add
BLAST

Proteomic databases

PaxDbiQ99MV5.
PRIDEiQ99MV5.

PTM databases

iPTMnetiQ99MV5.
PhosphoSiteiQ99MV5.

Expressioni

Tissue specificityi

Isoform 1: Specifically expressed in testis (PubMed:12754203). Isoform 1: In testis, present in pachytene spermatocytes but absent in postmeiotic spermatids (at protein level) (PubMed:20534472, PubMed:20547853). Isoform 2: Present in cardiomyocytes (at protein level) (PubMed:11279525). Isoform 2: Heart specific (PubMed:11854500). Isoform 3: Heart specific and is specifically expressed in cardiac myocytes (PubMed:12754203).4 Publications

Developmental stagei

Isoform 2: Expression is first observed in the linear heart tube at E8. The highest expression is in the region that will give rise to the ventricular segments. At E9.5, the ventricular expression is maintained in the looped heart tube. In the adult, expression is observed exclusively within myocardial cells.1 Publication

Inductioni

Isoform 2: Activated by MEF2C. Isoform 3: Activated by Nkx2-5.1 Publication

Gene expression databases

CleanExiMM_MOV10L1.

Interactioni

Subunit structurei

Interacts with PIWIL1 (PubMed:20534472). Interacts with PIWIL2 (PubMed:20534472, PubMed:20547853). Interacts with PIWIL4 (PubMed:20534472, PubMed:20547853). Interacts with HSPA2 (PubMed:20547853). Interacts with PLD6 (PubMed:25762440).3 Publications

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000015509.

Structurei

3D structure databases

ProteinModelPortaliQ99MV5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati642 – 652111Add
BLAST
Repeati653 – 663112Add
BLAST
Repeati664 – 674113Add
BLAST
Repeati675 – 685114Add
BLAST
Repeati686 – 696115Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni642 – 696555 X 11 AA tandem repeats of [TI]-R-N-[DN]-[GS]-Q-[SP]-I-T-[NK]-[IVN]Add
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi888 – 8914DEAG box

Sequence similaritiesi

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG1804. Eukaryota.
COG1112. LUCA.
HOGENOMiHOG000015363.
HOVERGENiHBG052382.
InParanoidiQ99MV5.
KOiK13983.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR027417. P-loop_NTPase.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Note: Additional isoforms seem to exist.

Isoform 1 (identifier: Q99MV5-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MIDDLIYFSN DAVTSTVLLN VGQEVIAVVE ENKVSNGLKA IRVEAVSDKW
60 70 80 90 100
EDDSKNSSKG LSDSSPRVLI GCVTSMLEGA GYISQTTYFS LESVCEGFHP
110 120 130 140 150
CKGDWVEAEY WIRPGTWSSE AISVKPLRYK RVDKVCISSL CGRNGVIEDS
160 170 180 190 200
IFFSLDSLKL PEGYIPRRHD IVNAVVVESS QSCYIWRALC MTPVKRDATL
210 220 230 240 250
GEAPQEPYGA LLLKNKGDIE VTRMTSFGTL KEGESKSIVI WIENKGKFSR
260 270 280 290 300
ELVSCRLANW DKAHQFRFET QGRSKSCPGA AAGSVPEGEN VNSLNHHRED
310 320 330 340 350
KTDEIPESRL ANSTEISPDG CACKEESREK GNTPEKQEPE PGGLIPPGEK
360 370 380 390 400
THIVVTCSAK NPGRCKELLL LCFSDFLIGR HLEVSVVSSE EALIAVREPF
410 420 430 440 450
SWKKPKSSQT LVSAKTTVVV TTQKRNSRRQ LPSFLPQYPI PDRLKKCVEQ
460 470 480 490 500
KIDILTFQPL LAELLNMSNY KEKFSTLLWL EEIHAEIELK EYNMSRVVLK
510 520 530 540 550
RKGDLLVLEV PGLAESRPSL YAGDKLILKS QEYNGHVIEY IGYVMEIHEE
560 570 580 590 600
DVTLKLNPGF EQMYNFEPMD VEFTYNRTTS RRCHYALEQV IHLGVKVLFP
610 620 630 640 650
EEIILQSPQV TGNWSLAQDT KNDGQSITNI TRNDGQSMTK VTRNDSQSIT
660 670 680 690 700
NIIRNDGQSI TNVTRNDGQP ITKVTRNNSQ SITNITRNDG QPITKNKKTV
710 720 730 740 750
KDQTKHTTEE RHVGTTDQPE KASSTAETMD EIQIPKARDK EFFNPVLNEN
760 770 780 790 800
QKLAVRRILS GDCRPLPYIL FGPPGTGKTV TIIEAVLQVH YALPDSRILV
810 820 830 840 850
CAPSNSAADL VCLRLHESKV PKPAAMVRVN ATCRFEETII DAIKPYCRDG
860 870 880 890 900
EDIWRASRFR IIITTCSSAG LFYQIGVRVG YFTHVFVDEA GQASEPECLI
910 920 930 940 950
PLGLISDING QIVLAGDPMQ LGPVIKSRLA MAYGLNVSML ERLMSRPAYL
960 970 980 990 1000
RDENAFGACG AYNPLLVTKL VKNYRSHSAL LALPSRLFYH RELEVCADPK
1010 1020 1030 1040 1050
VVTSLLGWEK LPRKGFPLIF HGVRGNEARE GRSPSWFSPA EAVQVMRYCC
1060 1070 1080 1090 1100
LLARSVSSQV SSKDIGVITP YRKQVEKIKI LLRNVDLTDI KVGSVEEFQG
1110 1120 1130 1140 1150
QEYLVIVIST VRSNEDRFED DRYFLGFLSN SKRFNVAITR PKALLIILGN
1160 1170 1180
PHVLVRDPCF GALLEYSVSN GVYTGCDLPP ELQALQK
Length:1,187
Mass (Da):132,792
Last modified:June 1, 2001 - v1
Checksum:i6A3A455DE3C0CB00
GO
Isoform 2 (identifier: Q99MV5-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-637: Missing.

Show »
Length:550
Mass (Da):61,311
Checksum:iC5AC4AA205DFDF4D
GO
Isoform 3 (identifier: Q99MV5-3) [UniParc]FASTAAdd to basket

Also known as: Csm1 Publication

, Cardiac-specific isoform of Mov10l11 Publication

The sequence of this isoform differs from the canonical sequence as follows:
     1-825: Missing.

Show »
Length:362
Mass (Da):40,547
Checksum:iA29D382E56DC089C
GO

Sequence cautioni

The sequence AAP60176.1 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti821 – 8211P → L (PubMed:11397016).Curated
Sequence conflicti821 – 8211P → L (PubMed:16141072).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 825825Missing in isoform 3. 1 PublicationVSP_010949Add
BLAST
Alternative sequencei1 – 637637Missing in isoform 2. 1 PublicationVSP_003392Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF285587 mRNA. Translation: AAK31966.1.
AF340211 mRNA. Translation: AAK77049.1.
AY303754 mRNA. Translation: AAP60176.1. Different initiation.
AK084786 mRNA. Translation: BAC39279.1.
CCDSiCCDS27737.1. [Q99MV5-1]
RefSeqiNP_112550.2. NM_031260.2.
XP_006521621.1. XM_006521558.2. [Q99MV5-3]
UniGeneiMm.358791.

Genome annotation databases

GeneIDi83456.
KEGGimmu:83456.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF285587 mRNA. Translation: AAK31966.1.
AF340211 mRNA. Translation: AAK77049.1.
AY303754 mRNA. Translation: AAP60176.1. Different initiation.
AK084786 mRNA. Translation: BAC39279.1.
CCDSiCCDS27737.1. [Q99MV5-1]
RefSeqiNP_112550.2. NM_031260.2.
XP_006521621.1. XM_006521558.2. [Q99MV5-3]
UniGeneiMm.358791.

3D structure databases

ProteinModelPortaliQ99MV5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000015509.

PTM databases

iPTMnetiQ99MV5.
PhosphoSiteiQ99MV5.

Proteomic databases

PaxDbiQ99MV5.
PRIDEiQ99MV5.

Protocols and materials databases

DNASUi83456.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi83456.
KEGGimmu:83456.

Organism-specific databases

CTDi54456.
MGIiMGI:1891384. Mov10l1.

Phylogenomic databases

eggNOGiKOG1804. Eukaryota.
COG1112. LUCA.
HOGENOMiHOG000015363.
HOVERGENiHBG052382.
InParanoidiQ99MV5.
KOiK13983.

Enzyme and pathway databases

ReactomeiR-MMU-5601884. PIWI-interacting RNA (piRNA) biogenesis.

Miscellaneous databases

PROiQ99MV5.
SOURCEiSearch...

Gene expression databases

CleanExiMM_MOV10L1.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR027417. P-loop_NTPase.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "An abundance of X-linked genes expressed in spermatogonia."
    Wang P.J., McCarrey J.R., Yang F., Page D.C.
    Nat. Genet. 27:422-426(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY.
    Tissue: Testis.
  2. Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
    Strain: NIH Swiss.
    Tissue: Heart.
  3. "Csm, a cardiac-specific isoform of the RNA helicase Mov10l1, is regulated by Nkx2.5 in embryonic heart."
    Ueyama T., Kasahara H., Ishiwata T., Yamasaki N., Izumo S.
    J. Biol. Chem. 278:28750-28757(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3), INDUCTION, TISSUE SPECIFICITY.
    Strain: NIH Swiss.
  4. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 769-1187.
    Strain: C57BL/6J.
    Tissue: Heart.
  5. "Suppression of proliferation and cardiomyocyte hypertrophy by CHAMP, a cardiac-specific RNA helicase."
    Liu Z.-P., Olson E.N.
    Proc. Natl. Acad. Sci. U.S.A. 99:2043-2048(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION (ISOFORM 2), SUBCELLULAR LOCATION (ISOFORM 2), MUTAGENESIS OF 888-ASP--GLN-892, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
  6. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Testis.
  7. "Mouse MOV10L1 associates with Piwi proteins and is an essential component of the Piwi-interacting RNA (piRNA) pathway."
    Zheng K., Xiol J., Reuter M., Eckardt S., Leu N.A., McLaughlin K.J., Stark A., Sachidanandam R., Pillai R.S., Wang P.J.
    Proc. Natl. Acad. Sci. U.S.A. 107:11841-11846(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE, INTERACTION WITH PIWIL1; PIWIL2 AND PIWIL4.
  8. "MOV10L1 is necessary for protection of spermatocytes against retrotransposons by Piwi-interacting RNAs."
    Frost R.J., Hamra F.K., Richardson J.A., Qi X., Bassel-Duby R., Olson E.N.
    Proc. Natl. Acad. Sci. U.S.A. 107:11847-11852(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE, INTERACTION WITH HSPA2; PIWIL2 AND PIWIL4.
  9. "Blockade of pachytene piRNA biogenesis reveals a novel requirement for maintaining post-meiotic germline genome integrity."
    Zheng K., Wang P.J.
    PLoS Genet. 8:E1003038-E1003038(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, DISRUPTION PHENOTYPE.
  10. "The RNA helicase MOV10L1 binds piRNA precursors to initiate piRNA processing."
    Vourekas A., Zheng K., Fu Q., Maragkakis M., Alexiou P., Ma J., Pillai R.S., Mourelatos Z., Wang P.J.
    Genes Dev. 29:617-629(2015) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, CATALYTIC ACTIVITY, RNA-BINDING, INTERACTION WITH PLD6, MUTAGENESIS OF LYS-778.

Entry informationi

Entry nameiM10L1_MOUSE
AccessioniPrimary (citable) accession number: Q99MV5
Secondary accession number(s): Q7TPA9, Q8C3W0, Q924C2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 2, 2002
Last sequence update: June 1, 2001
Last modified: June 8, 2016
This is version 107 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.