Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q99MR9

- PPR3A_MOUSE

UniProt

Q99MR9 - PPR3A_MOUSE

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Protein phosphatase 1 regulatory subunit 3A

Gene

Ppp1r3a

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Seems to act as a glycogen-targeting subunit for PP1. PP1 is essential for cell division, and participates in the regulation of glycogen metabolism, muscle contractility and protein synthesis. Plays an important role in glycogen synthesis but is not essential for insulin activation of glycogen synthase.1 Publication

GO - Molecular functioni

  1. protein serine/threonine phosphatase activity Source: MGI

GO - Biological processi

  1. dephosphorylation Source: GOC
  2. glycogen metabolic process Source: MGI
Complete GO annotation...

Keywords - Biological processi

Carbohydrate metabolism, Glycogen metabolism

Protein family/group databases

CAZyiCBM21. Carbohydrate-Binding Module Family 21.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein phosphatase 1 regulatory subunit 3A
Alternative name(s):
Protein phosphatase 1 glycogen-associated regulatory subunit
Protein phosphatase type-1 glycogen targeting subunit
Short name:
RG1
Gene namesi
Name:Ppp1r3a
Synonyms:Pp1g
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 6

Organism-specific databases

MGIiMGI:2153588. Ppp1r3a.

Subcellular locationi

Membrane By similarity; Single-pass membrane protein By similarity

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei1047 – 106721HelicalSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10891089Protein phosphatase 1 regulatory subunit 3APRO_0000071501Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei40 – 401Phosphoserine; by GSK3By similarity
Modified residuei44 – 441Phosphoserine; by GSK3By similarity
Modified residuei48 – 481Phosphoserine; by PKA and ISPKBy similarity
Modified residuei67 – 671Phosphoserine; by PKABy similarity

Post-translational modificationi

Phosphorylation at Ser-48 by ISPK stimulates the dephosphorylation of glycogen synthase and phosphorylase kinase.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ99MR9.
PaxDbiQ99MR9.
PRIDEiQ99MR9.

PTM databases

PhosphoSiteiQ99MR9.

Expressioni

Tissue specificityi

Skeletal muscle and heart.1 Publication

Gene expression databases

BgeeiQ99MR9.
GenevestigatoriQ99MR9.

Interactioni

Subunit structurei

Interacts with PPP1CC catalytic subunit of PP1, and associates with glycogen.By similarity

Protein-protein interaction databases

IntActiQ99MR9. 2 interactions.
MINTiMINT-4108485.

Structurei

3D structure databases

ProteinModelPortaliQ99MR9.
SMRiQ99MR9. Positions 102-235.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini123 – 231109CBM21PROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi64 – 674PP1-binding motif

Domaini

The CBM21 domain is known to be involved in the localization to glycogen and is characteristic of some regulatory subunit of phosphatase complexes.

Sequence similaritiesi

Contains 1 CBM21 (carbohydrate binding type-21) domain.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG288354.
GeneTreeiENSGT00530000062978.
HOGENOMiHOG000115667.
HOVERGENiHBG053657.
InParanoidiQ99MR9.
KOiK07189.
OMAiAFDPHEG.
OrthoDBiEOG769ZKR.
TreeFamiTF105537.

Family and domain databases

InterProiIPR005036. CBM_21.
[Graphical view]
PfamiPF03370. CBM_21. 1 hit.
[Graphical view]
PROSITEiPS51159. CBM21. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q99MR9-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MEPAEEPGQI SKDNFLEVPN LSDSVCEDEE VKATFKPGFS PQPSRRGSGS
60 70 80 90 100
SEDMYLDTPT SASRRVSFAD SLGFSLVSVK EFDCWELPSV STDFDLSGDV
110 120 130 140 150
FHTDEYVLSP LFDLPSSKEK LMEQLQVQKA VLESAEHLPG SSMKGIIRVL
160 170 180 190 200
NISFEKLVYV RMSLDDWQTH YDILAEYVPN SCDGETDQFS FKISLVPPYQ
210 220 230 240 250
KEGGKVEFCI RYETSAGTFW SNNNGTNYIL VCQKKRKEPE PVKPLEEAPS
260 270 280 290 300
RQIKGCLKVK SRSKEEPLLA PEENKFETLK FTESYIPTII CSHEDKDDLG
310 320 330 340 350
ANHPNVDDIN KKHDEHNGKE LDLMINQRLI TSQDEKNTFA TDTVNFTNKA
360 370 380 390 400
EGSEKKQAYH EINTDLFMGP LSPSLSAESS LKRDFYHSRS SSPGNEYGHP
410 420 430 440 450
HSEEIISDMG EKGPSLGDTS SDELMQLELC SKEDLDDNAN PANGSGRVCS
460 470 480 490 500
SFDQRMACGL KNNEAGIKKT GIQDYKYSHG DSTKLEESNA SSRDDYAKVD
510 520 530 540 550
NKKEKQTCLG VNENPSKNFQ SVFQTQEGHM GYPKISTEGD KANNQDLTSL
560 570 580 590 600
LSKDITANTW AVTVDPCPST NAKRSWREVG SGSNLEPGTS DLSSPRNFSP
610 620 630 640 650
LTDDHLFQAD RENSDSSNPE NQNMNTRHRK KWNVLETQSE TSETESDIAK
660 670 680 690 700
HTKEQAEYKD MWEKTDNSRN LKATPTEHLF TCRETECYGL SSLADHGITE
710 720 730 740 750
KAQAVTAYII KTTLESTPES ASARGKAIIA KLPQETAGND RPIEVKETAF
760 770 780 790 800
DPHEGRKDDS HYSLCHGDTA GVIHDNDFER ESHLDICNLR VDEMKKEKTT
810 820 830 840 850
STCFPQKTYD KEKHGIGSVT SIDEPSQVIT GNQKATSKLD LHLGVLPTDR
860 870 880 890 900
AIFQANADLE LLQELSRRTD FNAVPSAFNS DTASASRDSS QVYRHCSKKS
910 920 930 940 950
VPSYGEEKAV TNTTLQSIPT KSEYNWHPES EVLGHAMSKP EDVFKSSEIM
960 970 980 990 1000
KSGSGGERGG GPILQQKEGS LENSQGPMFF TNEPLENLDE ASSENEGLMH
1010 1020 1030 1040 1050
SGQSQCYLGD KGLVSSASAT VSTQELEAQG RESLLSISTN SKIPYFLLFL
1060 1070 1080
IFLATVYYYD LMIGLAFYLF SLYWLYWEGG RQRESVKKK
Length:1,089
Mass (Da):121,435
Last modified:July 27, 2011 - v2
Checksum:i85EC67FD90CC8FD2
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti959 – 9591G → S in AAK31072. (PubMed:11361130)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF309629, AF309628 Genomic DNA. Translation: AAK31072.1.
CH466533 Genomic DNA. Translation: EDL13902.1.
BC109007 mRNA. Translation: AAI09008.1.
AK084518 mRNA. Translation: BAC39208.2.
AK084719 mRNA. Translation: BAC39262.2.
CCDSiCCDS19917.1.
RefSeqiNP_536712.2. NM_080464.2.
UniGeneiMm.209429.

Genome annotation databases

EnsembliENSMUST00000045096; ENSMUSP00000049054; ENSMUSG00000042717.
GeneIDi140491.
KEGGimmu:140491.
UCSCiuc009ayw.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF309629 , AF309628 Genomic DNA. Translation: AAK31072.1 .
CH466533 Genomic DNA. Translation: EDL13902.1 .
BC109007 mRNA. Translation: AAI09008.1 .
AK084518 mRNA. Translation: BAC39208.2 .
AK084719 mRNA. Translation: BAC39262.2 .
CCDSi CCDS19917.1.
RefSeqi NP_536712.2. NM_080464.2.
UniGenei Mm.209429.

3D structure databases

ProteinModelPortali Q99MR9.
SMRi Q99MR9. Positions 102-235.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi Q99MR9. 2 interactions.
MINTi MINT-4108485.

Protein family/group databases

CAZyi CBM21. Carbohydrate-Binding Module Family 21.

PTM databases

PhosphoSitei Q99MR9.

Proteomic databases

MaxQBi Q99MR9.
PaxDbi Q99MR9.
PRIDEi Q99MR9.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000045096 ; ENSMUSP00000049054 ; ENSMUSG00000042717 .
GeneIDi 140491.
KEGGi mmu:140491.
UCSCi uc009ayw.1. mouse.

Organism-specific databases

CTDi 5506.
MGIi MGI:2153588. Ppp1r3a.

Phylogenomic databases

eggNOGi NOG288354.
GeneTreei ENSGT00530000062978.
HOGENOMi HOG000115667.
HOVERGENi HBG053657.
InParanoidi Q99MR9.
KOi K07189.
OMAi AFDPHEG.
OrthoDBi EOG769ZKR.
TreeFami TF105537.

Miscellaneous databases

NextBioi 369808.
PROi Q99MR9.
SOURCEi Search...

Gene expression databases

Bgeei Q99MR9.
Genevestigatori Q99MR9.

Family and domain databases

InterProi IPR005036. CBM_21.
[Graphical view ]
Pfami PF03370. CBM_21. 1 hit.
[Graphical view ]
PROSITEi PS51159. CBM21. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Gene structure and expression of the targeting subunit, RGL, of the muscle-specific glycogen-associated type 1 protein phosphatase, PP1G."
    Lanner C., Suzuki Y., Bi C., Zhang H., Cooper L.D., Bowker-Kinley M.M., DePaoli-Roach A.A.
    Arch. Biochem. Biophys. 388:135-145(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], TISSUE SPECIFICITY.
    Strain: 129/Sv.
  2. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  4. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-626.
    Strain: C57BL/6J.
    Tissue: Embryonic heart.
  5. Lubec G., Sunyer B., Chen W.-Q.
    Submitted (JAN-2009) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 259-264, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: OF1.
    Tissue: Hippocampus.
  6. "Insulin control of glycogen metabolism in knockout mice lacking the muscle-specific protein phosphatase PP1G/RGL."
    Suzuki Y., Lanner C., Kim J.-H., Vilardo P.G., Zhang H., Yang J., Cooper L.D., Steele M., Kennedy A., Bock C.B., Scrimgeour A., Lawrence J.C. Jr., DePaoli-Roach A.A.
    Mol. Cell. Biol. 21:2683-2694(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
    Strain: 129/Sv.

Entry informationi

Entry nameiPPR3A_MOUSE
AccessioniPrimary (citable) accession number: Q99MR9
Secondary accession number(s): Q32MS0, Q8BUJ4, Q8BUL0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 13, 2004
Last sequence update: July 27, 2011
Last modified: October 29, 2014
This is version 89 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3