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Protein

Serrate RNA effector molecule homolog

Gene

Srrt

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Acts as a mediator between the cap-binding complex (CBC) and the primary microRNAs (miRNAs) processing machinery during cell proliferation. Contributes to the stability and delivery of capped primary miRNA transcripts to the primary miRNA processing complex containing DGCR8 and DROSHA, thereby playing a role in RNA-mediated gene silencing (RNAi) by miRNAs. Binds capped RNAs (m7GpppG-capped RNA); however interaction is probably mediated via its interaction with NCBP1/CBP80 component of the CBC complex. Involved in cell cycle progression at S phase. Does not directly confer arsenite resistance but rather modulates arsenic sensitivity. Independently of its activity on miRNAs, necessary and sufficient to promote neural stem cell self-renewal. Does so by directly binding SOX2 promoter and positively regulating its transcription.2 Publications

GO - Molecular functioni

  • DNA binding Source: UniProtKB
  • nucleotide binding Source: InterPro
  • poly(A) RNA binding Source: MGI

GO - Biological processi

  • cell proliferation Source: UniProtKB
  • neuronal stem cell population maintenance Source: UniProtKB
  • positive regulation of neurogenesis Source: CACAO
  • primary miRNA processing Source: UniProtKB
  • regulation of transcription, DNA-templated Source: UniProtKB
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

RNA-mediated gene silencing, Transcription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-MMU-6807505. RNA polymerase II transcribes snRNA genes.
R-MMU-72163. mRNA Splicing - Major Pathway.

Names & Taxonomyi

Protein namesi
Recommended name:
Serrate RNA effector molecule homolog
Alternative name(s):
Arsenite-resistance protein 2
Gene namesi
Name:Srrt
Synonyms:Ars2, Asr2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:1933527. Srrt.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB
  • nucleoplasm Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Disruption phenotypei

Death around the time of implantation. Deletion in adults leads to proliferative arrest and bone marrow hypoplasia whereas parenchymal organs composed of nonproliferating cells are unaffected.2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00002209662 – 875Serrate RNA effector molecule homologAdd BLAST874

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylglycineBy similarity1
Modified residuei4PhosphoserineBy similarity1
Modified residuei8PhosphotyrosineBy similarity1
Modified residuei67PhosphoserineBy similarity1
Modified residuei74PhosphoserineBy similarity1
Modified residuei136PhosphoserineBy similarity1
Modified residuei492PhosphoserineBy similarity1
Modified residuei539PhosphoserineBy similarity1
Modified residuei543PhosphothreonineCombined sources1
Modified residuei569PhosphoserineBy similarity1
Modified residuei670PhosphothreonineBy similarity1
Modified residuei678PhosphoserineBy similarity1
Modified residuei832Omega-N-methylarginineBy similarity1
Modified residuei839Omega-N-methylarginineBy similarity1
Modified residuei849Omega-N-methylarginineBy similarity1

Keywords - PTMi

Acetylation, Methylation, Phosphoprotein

Proteomic databases

PaxDbiQ99MR6.
PeptideAtlasiQ99MR6.
PRIDEiQ99MR6.

PTM databases

iPTMnetiQ99MR6.
PhosphoSitePlusiQ99MR6.
SwissPalmiQ99MR6.

Expressioni

Tissue specificityi

Widely expressed, with a preference for proliferating cells. Highly expressed in hematopoietic tissues and reduced or absent expression in parenchymal organs like liver and kidney. In the brain, expressed in the subventricular zone by niche astrocytes, ependymal cells and neural stem cells. In this cerebral context, expressed in slowly dividing cells.3 Publications

Inductioni

Upon cell proliferation.1 Publication

Gene expression databases

BgeeiENSMUSG00000037364.
CleanExiMM_ARS2.
ExpressionAtlasiQ99MR6. baseline and differential.
GenevisibleiQ99MR6. MM.

Interactioni

Subunit structurei

Interacts with CASP8AP2 and ERBB4 (By similarity). Interacts with NCBP1/CBP80 and DROSHA (PubMed:19632182). Interacts with LUZP4 (By similarity). Interacts with NCBP2/CBP20 and NCBP3 (By similarity).By similarity1 Publication

Protein-protein interaction databases

BioGridi219965. 2 interactors.
IntActiQ99MR6. 2 interactors.
MINTiMINT-1853620.
STRINGi10090.ENSMUSP00000043123.

Structurei

3D structure databases

ProteinModelPortaliQ99MR6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi10 – 82Arg-richAdd BLAST73
Compositional biasi258 – 401Glu-richAdd BLAST144
Compositional biasi759 – 827Pro-richAdd BLAST69

Sequence similaritiesi

Belongs to the ARS2 family.Curated

Phylogenomic databases

eggNOGiKOG2295. Eukaryota.
ENOG410XR8S. LUCA.
GeneTreeiENSGT00390000005492.
InParanoidiQ99MR6.
OMAiDHGGDPY.
OrthoDBiEOG091G06J6.
PhylomeDBiQ99MR6.
TreeFamiTF317609.

Family and domain databases

InterProiIPR007042. Arsenite-R_2.
IPR021933. DUF3546.
IPR012677. Nucleotide-bd_a/b_plait.
[Graphical view]
PfamiPF04959. ARS2. 1 hit.
PF12066. DUF3546. 1 hit.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 1 hit.

Sequences (4)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform A (identifier: Q99MR6-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGDSDDEYDR RRRDKFRRER SDYDRSRERD ERRRGDDWND REWDRGRERR
60 70 80 90 100
SRGEYRDYDR NRRERFSPPR HELSPPQKRM RRDWDEHSSD PYHSGYDMPY
110 120 130 140 150
AGGGGGPTYG PPQPWGHPDV HIMQHHVLPI QARLGSIAEI DLGVPPPIMK
160 170 180 190 200
SFKEFLLSLD DSVDETEAVK RYNDYKLDFR RQQMQDFFLA HKDEEWFRSK
210 220 230 240 250
YHPDEVGKRR QEARGALQNR LKVFLSLMES GWFDNLLLDI DKADAIVKML
260 270 280 290 300
DAAVIKMEGG TENDLRILEQ EEEEEQAGKT GEASKKEEAR AGPALGEGER
310 320 330 340 350
KANDKDEKKE DGKQAENDSS NDDKTKKSEG DGDKEEKKEE AEKEAKKSKK
360 370 380 390 400
RNRKQSGDDS FDEGSVSESE SESEGGQAEE EKEEAEEALK EKEKPKEEEK
410 420 430 440 450
EKPKDAAGLE CKPRPLHKTC SLFMRNIAPN ISRAEIISLC KRYPGFMRVA
460 470 480 490 500
LSEPQPERRF FRRGWVTFDR SVNIKEICWN LQNIRLRECE LSPGVNRDLT
510 520 530 540 550
RRVRNINGIT QHKQIVRNDI KLAAKLIHTL DDRTQLWASE PGTPPVPTSL
560 570 580 590 600
PSQNPILKNI TDYLIEEVSA EEEELLGSSG GPPPEEPPKE GNPAEINVER
610 620 630 640 650
DEKLIKVLDK LLLYLRIVHS LDYYNTCEYP NEDEMPNRCG IIHVRGPMPP
660 670 680 690 700
NRISHGEVLE WQKTFEEKLT PLLSVRESLS EEEAQKMGRK DPEQEVEKFV
710 720 730 740 750
TSNTQELGKD KWLCPLSGKK FKGPEFVRKH IFNKHAEKIE EVKKEVAFFN
760 770 780 790 800
NFLTDAKRPA LPEIKPAQPP GPAQILPPGL TPGLPYPHQT PQGLMPYGQP
810 820 830 840 850
RPPILGYGAG AVRPAVPTGG PPYPHAPYGA GRGNYDAFRG QGGYPGKPRN
860 870
RMVRGDPRAI VEYRDLDAPD DVDFF
Length:875
Mass (Da):100,452
Last modified:June 1, 2001 - v1
Checksum:i9571445674452886
GO
Isoform B (identifier: Q99MR6-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     775-779: ILPPG → S

Show »
Length:871
Mass (Da):100,062
Checksum:i76B1DA0A00985DCC
GO
Isoform C (identifier: Q99MR6-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     775-779: ILPPG → S
     809-815: Missing.

Show »
Length:864
Mass (Da):99,439
Checksum:i8BE9BBCAA78C162F
GO
Isoform D (identifier: Q99MR6-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     809-815: Missing.

Show »
Length:868
Mass (Da):99,830
Checksum:i7141CAF88A6802EE
GO

Sequence cautioni

The sequence AAH19117 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence BAE29458 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti567E → G in BAE29458 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_000325775 – 779ILPPG → S in isoform B and isoform C. 1 Publication5
Alternative sequenceiVSP_000326809 – 815Missing in isoform C and isoform D. 2 Publications7

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF312033 Genomic DNA. Translation: AAK28817.1.
AF312033 Genomic DNA. Translation: AAK28818.1.
AF312033 Genomic DNA. Translation: AAK28819.1.
AF312033 Genomic DNA. Translation: AAK28820.1.
BC019117 mRNA. Translation: AAH19117.1. Different initiation.
BC066831 mRNA. Translation: AAH66831.1.
AK150310 mRNA. Translation: BAE29458.1. Different initiation.
CCDSiCCDS39331.1. [Q99MR6-1]
CCDS80439.1. [Q99MR6-3]
CCDS80440.1. [Q99MR6-4]
RefSeqiNP_001103379.1. NM_001109909.1. [Q99MR6-4]
NP_001103380.1. NM_001109910.1. [Q99MR6-3]
NP_113582.1. NM_031405.2. [Q99MR6-1]
XP_006504692.1. XM_006504629.1. [Q99MR6-2]
UniGeneiMm.387734.

Genome annotation databases

EnsembliENSMUST00000040873; ENSMUSP00000043123; ENSMUSG00000037364. [Q99MR6-1]
ENSMUST00000197466; ENSMUSP00000142564; ENSMUSG00000037364. [Q99MR6-3]
ENSMUST00000199243; ENSMUSP00000143232; ENSMUSG00000037364. [Q99MR6-4]
GeneIDi83701.
KEGGimmu:83701.
UCSCiuc009acb.2. mouse. [Q99MR6-1]
uc009acc.2. mouse. [Q99MR6-3]
uc012eew.1. mouse. [Q99MR6-4]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF312033 Genomic DNA. Translation: AAK28817.1.
AF312033 Genomic DNA. Translation: AAK28818.1.
AF312033 Genomic DNA. Translation: AAK28819.1.
AF312033 Genomic DNA. Translation: AAK28820.1.
BC019117 mRNA. Translation: AAH19117.1. Different initiation.
BC066831 mRNA. Translation: AAH66831.1.
AK150310 mRNA. Translation: BAE29458.1. Different initiation.
CCDSiCCDS39331.1. [Q99MR6-1]
CCDS80439.1. [Q99MR6-3]
CCDS80440.1. [Q99MR6-4]
RefSeqiNP_001103379.1. NM_001109909.1. [Q99MR6-4]
NP_001103380.1. NM_001109910.1. [Q99MR6-3]
NP_113582.1. NM_031405.2. [Q99MR6-1]
XP_006504692.1. XM_006504629.1. [Q99MR6-2]
UniGeneiMm.387734.

3D structure databases

ProteinModelPortaliQ99MR6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi219965. 2 interactors.
IntActiQ99MR6. 2 interactors.
MINTiMINT-1853620.
STRINGi10090.ENSMUSP00000043123.

PTM databases

iPTMnetiQ99MR6.
PhosphoSitePlusiQ99MR6.
SwissPalmiQ99MR6.

Proteomic databases

PaxDbiQ99MR6.
PeptideAtlasiQ99MR6.
PRIDEiQ99MR6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000040873; ENSMUSP00000043123; ENSMUSG00000037364. [Q99MR6-1]
ENSMUST00000197466; ENSMUSP00000142564; ENSMUSG00000037364. [Q99MR6-3]
ENSMUST00000199243; ENSMUSP00000143232; ENSMUSG00000037364. [Q99MR6-4]
GeneIDi83701.
KEGGimmu:83701.
UCSCiuc009acb.2. mouse. [Q99MR6-1]
uc009acc.2. mouse. [Q99MR6-3]
uc012eew.1. mouse. [Q99MR6-4]

Organism-specific databases

CTDi51593.
MGIiMGI:1933527. Srrt.

Phylogenomic databases

eggNOGiKOG2295. Eukaryota.
ENOG410XR8S. LUCA.
GeneTreeiENSGT00390000005492.
InParanoidiQ99MR6.
OMAiDHGGDPY.
OrthoDBiEOG091G06J6.
PhylomeDBiQ99MR6.
TreeFamiTF317609.

Enzyme and pathway databases

ReactomeiR-MMU-6807505. RNA polymerase II transcribes snRNA genes.
R-MMU-72163. mRNA Splicing - Major Pathway.

Miscellaneous databases

ChiTaRSiSrrt. mouse.
PROiQ99MR6.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000037364.
CleanExiMM_ARS2.
ExpressionAtlasiQ99MR6. baseline and differential.
GenevisibleiQ99MR6. MM.

Family and domain databases

InterProiIPR007042. Arsenite-R_2.
IPR021933. DUF3546.
IPR012677. Nucleotide-bd_a/b_plait.
[Graphical view]
PfamiPF04959. ARS2. 1 hit.
PF12066. DUF3546. 1 hit.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiSRRT_MOUSE
AccessioniPrimary (citable) accession number: Q99MR6
Secondary accession number(s): Q3UD04
, Q5D042, Q8VEE6, Q99MR4, Q99MR5, Q99MR7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 23, 2002
Last sequence update: June 1, 2001
Last modified: November 2, 2016
This is version 113 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.