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Q99MR3 (S12A9_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 86. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Solute carrier family 12 member 9
Alternative name(s):
Cation-chloride cotransporter-interacting protein 1
Potassium-chloride transporter 9
Gene names
Name:Slc12a9
Synonyms:Cip1, Slc12a8
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length914 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

May be an inhibitor of SLC12A1. Seems to correspond to a subunit of a multimeric transport system and thus, additional subunits may be required for its function By similarity.

Subunit structure

Interacts with SLC12A1 By similarity.

Subcellular location

Cell membrane; Multi-pass membrane protein By similarity.

Sequence similarities

Belongs to the SLC12A transporter family.

Ontologies

Keywords
   Biological processTransport
   Cellular componentCell membrane
Membrane
   DomainTransmembrane
Transmembrane helix
   PTMGlycoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processtransmembrane transport

Inferred from electronic annotation. Source: InterPro

   Cellular_componentintegral component of membrane

Inferred from sequence orthology Ref.1. Source: MGI

plasma membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 914914Solute carrier family 12 member 9
PRO_0000331416

Regions

Topological domain1 – 3636Cytoplasmic Potential
Transmembrane37 – 5721Helical; Potential
Topological domain58 – 7215Extracellular Potential
Transmembrane73 – 9321Helical; Potential
Topological domain94 – 11926Cytoplasmic Potential
Transmembrane120 – 14021Helical; Potential
Topological domain141 – 16727Extracellular Potential
Transmembrane168 – 18821Helical; Potential
Topological domain189 – 1935Cytoplasmic Potential
Transmembrane194 – 21421Helical; Potential
Topological domain215 – 26248Extracellular Potential
Transmembrane263 – 28321Helical; Potential
Topological domain284 – 29714Cytoplasmic Potential
Transmembrane298 – 31821Helical; Potential
Topological domain319 – 33820Extracellular Potential
Transmembrane339 – 35921Helical; Potential
Topological domain360 – 37617Cytoplasmic Potential
Transmembrane377 – 39923Helical; Potential
Topological domain400 – 41617Extracellular Potential
Transmembrane417 – 43721Helical; Potential
Topological domain438 – 46629Cytoplasmic Potential
Transmembrane467 – 48721Helical; Potential
Topological domain488 – 740253Extracellular Potential
Transmembrane741 – 76121Helical; Potential
Topological domain762 – 914153Cytoplasmic Potential
Compositional bias715 – 7184Poly-Ser
Compositional bias811 – 8166Poly-Glu

Amino acid modifications

Glycosylation2181N-linked (GlcNAc...); atypical Ref.5
Glycosylation2281N-linked (GlcNAc...) Ref.5
Glycosylation2431N-linked (GlcNAc...) Ref.5

Experimental info

Sequence conflict341S → A in AAK28822. Ref.1
Sequence conflict341S → A in AAL26866. Ref.2
Sequence conflict341S → A in AAH46982. Ref.4

Sequences

Sequence LengthMass (Da)Tools
Q99MR3 [UniParc].

Last modified July 27, 2011. Version 2.
Checksum: 769EA9EB4BD40E00

FASTA91496,330
        10         20         30         40         50         60 
MASESSPLLA YRLLGEEGAA FPPNGAGVSG VPSSRKLSTF LGVVVPTVLS MFSIVVFLRI 

        70         80         90        100        110        120 
GFVVGHAGLL QALAMLLVAY IILALTVLSV CAIATNGAVR GGGAYFMISR TLGPEVGGSI 

       130        140        150        160        170        180 
GLMFYLANVC GCAVSLLGLV ESILDVFGAD ATGSSGIQVL PQGYGWNLLY GSLLLGLVGG 

       190        200        210        220        230        240 
VCTLGAGLYA RASFLTFLLV SGSLASVLVS FVAVGPRNIP LAPRPGTNAS SVPHRHGHFT 

       250        260        270        280        290        300 
GFNGSTLRDN LGAGYAEDYT TGAMMTFASV FAVLFNGCTG IMAGANMSGE LKDPSRAIPL 

       310        320        330        340        350        360 
GTIIAVAYTF FIYILLFFLS SFTCDRALLQ EDYGFFRDIS LWPPLVLIGI YATALSASMS 

       370        380        390        400        410        420 
SLIGASRILH ALAQDDLFGV ILAPAKVVSG GGNPWGAVLY SWGLVQLVLL AGKLNTLAAV 

       430        440        450        460        470        480 
VTVFYLVAYA AVDLSCLSLE WASAPNFRPT FSLFSWHTCL LGVASCLLMM FLISPGAAGG 

       490        500        510        520        530        540 
SLLLMGLLSA LLTARGGPSS WGYVSQALLF HQVRKYLLRL DVRKEHVKFW RPQLLLLVGN 

       550        560        570        580        590        600 
PRGALPLLRL ANQLKKGGLY VLGHVTLGDL DSLPSDPVQP QYGAWLSLVD LAQVKAFVDL 

       610        620        630        640        650        660 
TLSPSVRQGA QHLLRISGLG GMKPNTLVLG FYDDAPPQDH FLTDPAFSEP AEGTREGGSP 

       670        680        690        700        710        720 
ALSTLFPPPR APGSPRALSP QDYVATVADA LKMNKNVVLA RACGALPPER LSRGSSSSAQ 

       730        740        750        760        770        780 
LHHVDVWPLN LLRPRGGPGY VDVCGLFLLQ MATILSMVPA WHSARLRIFL CLGPREAPGA 

       790        800        810        820        830        840 
AEGRLRALLS QLRIRAEVQE VVWGEGAETG EPEEEEGDFV NGGRGDEEAE ALACSANALV 

       850        860        870        880        890        900 
RAQQGRGTVG GPGGPEGRDG EEGPTTALTF LYLPRPPADP ARYPRYLALL ETLSRDLGPT 

       910 
LLIHGVTPVT CTDL 

« Hide

References

« Hide 'large scale' references
[1]"Comparative analysis of the gene-dense ACHE/TFR2 region on human chromosome 7q22 with the orthologous region on mouse chromosome 5."
Wilson M.D., Riemer C., Martindale D.W., Schnupf P., Boright A.P., Cheung T.L., Hardy D.M., Schwartz S., Scherer S.W., Tsui L.-C., Miller W., Koop B.F.
Nucleic Acids Res. 29:1352-1365(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 129/Sv.
[2]"Cloning of mouse Slc12a8, a new member of the cation-chloride cotransporter gene family."
Mount D.B.
Submitted (OCT-2000) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: FVB/N.
[3]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: FVB/N.
Tissue: Colon.
[5]"Mass-spectrometric identification and relative quantification of N-linked cell surface glycoproteins."
Wollscheid B., Bausch-Fluck D., Henderson C., O'Brien R., Bibel M., Schiess R., Aebersold R., Watts J.D.
Nat. Biotechnol. 27:378-386(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-218; ASN-228 AND ASN-243.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF312033 Genomic DNA. Translation: AAK28822.1.
AF314957 mRNA. Translation: AAL26866.1.
AC150682 Genomic DNA. No translation available.
BC046982 mRNA. Translation: AAH46982.1.
CCDSCCDS19766.1.
RefSeqNP_113583.2. NM_031406.3.
XP_006504696.1. XM_006504633.1.
UniGeneMm.235625.

3D structure databases

ProteinModelPortalQ99MR3.
ModBaseSearch...
MobiDBSearch...

PTM databases

PhosphoSiteQ99MR3.

Proteomic databases

PaxDbQ99MR3.
PRIDEQ99MR3.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000039991; ENSMUSP00000038106; ENSMUSG00000037344.
GeneID83704.
KEGGmmu:83704.
UCSCuc009ace.2. mouse.

Organism-specific databases

CTD56996.
MGIMGI:1933532. Slc12a9.

Phylogenomic databases

eggNOGCOG0531.
GeneTreeENSGT00560000077088.
HOGENOMHOG000018183.
HOVERGENHBG106223.
InParanoidQ99MR3.
KOK14429.
OMAGHFTGFN.
OrthoDBEOG7GN2M6.
TreeFamTF313191.

Gene expression databases

BgeeQ99MR3.
GenevestigatorQ99MR3.

Family and domain databases

InterProIPR004841. AA-permease/SLC12A_dom.
[Graphical view]
PfamPF00324. AA_permease. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio350746.
PROQ99MR3.
SOURCESearch...

Entry information

Entry nameS12A9_MOUSE
AccessionPrimary (citable) accession number: Q99MR3
Secondary accession number(s): E9QNN4
Entry history
Integrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: July 27, 2011
Last modified: July 9, 2014
This is version 86 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot