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Protein

Collagen alpha-1(XXV) chain

Gene

Col25a1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Inhibits fibrillization of beta amyloid peptide during the elongation phase. Has also been shown to assemble amyloid fibrils into protease-resistant aggregates. Binds heparin (By similarity).By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei112 – 1132Cleavage; by furinBy similarity

GO - Molecular functioni

  1. beta-amyloid binding Source: UniProtKB
  2. heparin binding Source: UniProtKB

GO - Biological processi

  1. axonogenesis involved in innervation Source: MGI
Complete GO annotation...

Keywords - Ligandi

Heparin-binding

Enzyme and pathway databases

ReactomeiREACT_198984. Collagen biosynthesis and modifying enzymes.
REACT_199055. Collagen degradation.

Names & Taxonomyi

Protein namesi
Recommended name:
Collagen alpha-1(XXV) chain
Alternative name(s):
CLAC-P
Cleaved into the following chain:
Gene namesi
Name:Col25a1Imported
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 3

Organism-specific databases

MGIiMGI:1924268. Col25a1.

Subcellular locationi

Membrane Curated; Single-pass type II membrane protein Curated
Note: After proteolytic cleavage, CLAC is secreted.By similarity

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 3333CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei34 – 5421Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
BLAST
Topological domaini55 – 666612ExtracellularSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. collagen trimer Source: UniProtKB-KW
  2. extracellular region Source: MGI
  3. extracellular space Source: UniProtKB
  4. integral component of plasma membrane Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Amyloid, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 666666Collagen alpha-1(XXV) chainPRO_0000259613Add
BLAST
Chaini113 – 654542Collagen-like Alzheimer amyloid plaque componentBy similarityPRO_0000259614Add
BLAST

Post-translational modificationi

Undergoes proteolytic cleavage by furin protease to yield the soluble collagen-like Alzheimer amyloid plaque component.By similarity
Glycosylated.By similarity
Hydroxylated on proline and lysine residues.By similarity

Keywords - PTMi

Glycoprotein, Hydroxylation

Proteomic databases

PRIDEiQ99MQ5.

PTM databases

PhosphoSiteiQ99MQ5.

Expressioni

Tissue specificityi

Expressed predominantly in neurons with low levels also detected in heart, testis and eye.1 Publication

Gene expression databases

CleanExiMM_COL25A1.
ExpressionAtlasiQ99MQ5. baseline and differential.
GenevestigatoriQ99MQ5.

Interactioni

Subunit structurei

Forms homodimers and homotrimers. Binds to the fibrillized forms of beta amyloid peptide 40 (beta-APP40) and beta amyloid peptide 42 (beta-APP42). Found associated with beta-APP42 more frequently than with beta-APP40 (By similarity).By similarity

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini121 – 16444Collagen-like 1Sequence AnalysisAdd
BLAST
Domaini192 – 24756Collagen-like 2Sequence AnalysisAdd
BLAST
Domaini249 – 30860Collagen-like 3Sequence AnalysisAdd
BLAST
Domaini311 – 37060Collagen-like 4Sequence AnalysisAdd
BLAST
Domaini373 – 42553Collagen-like 5Sequence AnalysisAdd
BLAST
Domaini455 – 51460Collagen-like 6Sequence AnalysisAdd
BLAST
Domaini529 – 58860Collagen-like 7Sequence AnalysisAdd
BLAST
Domaini589 – 64860Collagen-like 8Sequence AnalysisAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni181 – 1888Interaction with beta amyloid peptideBy similarity

Sequence similaritiesi

Contains 8 collagen-like domains.Sequence Analysis

Keywords - Domaini

Collagen, Repeat, Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG12793.
GeneTreeiENSGT00780000121854.
HOGENOMiHOG000085653.
HOVERGENiHBG101380.
InParanoidiQ99MQ5.
OMAiDENFFFP.
OrthoDBiEOG7D59P1.
TreeFamiTF338175.

Family and domain databases

InterProiIPR029575. CLAC-P.
IPR008160. Collagen.
[Graphical view]
PANTHERiPTHR24023:SF412. PTHR24023:SF412. 1 hit.
PfamiPF01391. Collagen. 6 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q99MQ5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLVKKLAGKG GGRESGSEDP RPLGQRCAGT MPSCTALATL LSVVAVAFCF
60 70 80 90 100
YLGVKTNDLQ ARIAALESAK GTPSFHPLSD TVDELKAMVQ EKVERLLAQK
110 120 130 140 150
SYEHMAKIRT AREAPLECNC PAGPPGKRGK RGRRGESGPP GQPGPQGPPG
160 170 180 190 200
PKGDKGEQGD QGPRMVFPKI NHGFLSADQQ LIKRRLIKGD QGQAGPPGPP
210 220 230 240 250
GPPGPRGPPG DTGKDGPRGM PGVPGEPGKP GEQGLMGPLG PPGQKGSIGA
260 270 280 290 300
PGTPGMDGQK GEPGSPGAAG QSGLPGPKGE PGKEGEKGDA GENGPKGDTG
310 320 330 340 350
EKGDPGSSAA GIKGEPGESG RPGQKGEPGL PGLPGLPGIK GEPGFIGPQG
360 370 380 390 400
EPGLPGLPGT KGDRGEAGPP GRGERGDPGA PGPKGKQGES GARGPKGSKG
410 420 430 440 450
DRGDKGDSGA LGPRGPPGQK GDPGATEIID YNGNLHEALQ RITTLTVTGP
460 470 480 490 500
PGPPGPQGLQ GPKGEQGSPG IPGVDGEQGL KGSKGDMGDP GVPGEKGGLG
510 520 530 540 550
LPGLPGANGV KGEKGDTGLP GPQGPSIIGP PGPPGPHGPP GPMGPHGLPG
560 570 580 590 600
PKGASGLDGK PGSRGADGPI GPHGPAGPKG ERGEKGAMGE PGPRGPYGLP
610 620 630 640 650
GKDGEPGLDG FPGPRGEKGD LGEKGEKGFR GVKGEKGEPG QPGLDGLDAP
660
CQLGPDGLPM PGCWQK
Length:666
Mass (Da):65,377
Last modified:October 3, 2012 - v2
Checksum:iDD8F5CAADE401366
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti64 – 641A → V in AAK37475 (PubMed:11927537).Curated
Sequence conflicti104 – 1041H → Y in AAK37475 (PubMed:11927537).Curated
Sequence conflicti111 – 1111A → V in AAK37475 (PubMed:11927537).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF315290 mRNA. Translation: AAK37475.1.
AC091312 Genomic DNA. No translation available.
AC110540 Genomic DNA. No translation available.
AC114642 Genomic DNA. No translation available.
AC164078 Genomic DNA. No translation available.
CH466532 Genomic DNA. Translation: EDL12220.1.
CCDSiCCDS38637.1.
RefSeqiNP_084114.2. NM_029838.4.
UniGeneiMm.91728.

Genome annotation databases

EnsembliENSMUST00000080335; ENSMUSP00000079210; ENSMUSG00000058897.
GeneIDi77018.
KEGGimmu:77018.
UCSCiuc008riw.2. mouse.
uc008riz.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF315290 mRNA. Translation: AAK37475.1.
AC091312 Genomic DNA. No translation available.
AC110540 Genomic DNA. No translation available.
AC114642 Genomic DNA. No translation available.
AC164078 Genomic DNA. No translation available.
CH466532 Genomic DNA. Translation: EDL12220.1.
CCDSiCCDS38637.1.
RefSeqiNP_084114.2. NM_029838.4.
UniGeneiMm.91728.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

PTM databases

PhosphoSiteiQ99MQ5.

Proteomic databases

PRIDEiQ99MQ5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000080335; ENSMUSP00000079210; ENSMUSG00000058897.
GeneIDi77018.
KEGGimmu:77018.
UCSCiuc008riw.2. mouse.
uc008riz.1. mouse.

Organism-specific databases

CTDi84570.
MGIiMGI:1924268. Col25a1.

Phylogenomic databases

eggNOGiNOG12793.
GeneTreeiENSGT00780000121854.
HOGENOMiHOG000085653.
HOVERGENiHBG101380.
InParanoidiQ99MQ5.
OMAiDENFFFP.
OrthoDBiEOG7D59P1.
TreeFamiTF338175.

Enzyme and pathway databases

ReactomeiREACT_198984. Collagen biosynthesis and modifying enzymes.
REACT_199055. Collagen degradation.

Miscellaneous databases

ChiTaRSiCol25a1. mouse.
NextBioi13888081.
PROiQ99MQ5.
SOURCEiSearch...

Gene expression databases

CleanExiMM_COL25A1.
ExpressionAtlasiQ99MQ5. baseline and differential.
GenevestigatoriQ99MQ5.

Family and domain databases

InterProiIPR029575. CLAC-P.
IPR008160. Collagen.
[Graphical view]
PANTHERiPTHR24023:SF412. PTHR24023:SF412. 1 hit.
PfamiPF01391. Collagen. 6 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "CLAC: a novel Alzheimer amyloid plaque component derived from a transmembrane precursor, CLAC-P/collagen type XXV."
    Hashimoto T., Wakabayashi T., Watanabe A., Kowa H., Hosoda R., Nakamura A., Kanazawa I., Arai T., Takio K., Mann D.M.A., Iwatsubo T.
    EMBO J. 21:1524-1534(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
    Strain: BALB/cImported.
    Tissue: BrainImported.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  3. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Entry informationi

Entry nameiCOPA1_MOUSE
AccessioniPrimary (citable) accession number: Q99MQ5
Secondary accession number(s): G3X9H6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 31, 2006
Last sequence update: October 3, 2012
Last modified: March 4, 2015
This is version 88 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.