Q99MQ5 (COPA1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Collagen alpha-1(XXV) chain

Alternative name(s):
CLAC-P

Cleaved into the following chain:

1. Collagen-like Alzheimer amyloid plaque component
   Short name=CLAC

Gene names

Name:

Col25a1

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	666 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Inhibits fibrillization of beta amyloid peptide during the elongation phase. Has also been shown to assemble amyloid fibrils into protease-resistant aggregates. Binds heparin By similarity. UniProtKB Q9BXS0
Subunit structure	Forms homodimers and homotrimers. Binds to the fibrillized forms of beta amyloid peptide 40 (beta-APP40) and beta amyloid peptide 42 (beta-APP42). Found associated with beta-APP42 more frequently than with beta-APP40 By similarity. UniProtKB Q9BXS0
Subcellular location	Membrane; Single-pass type II membrane protein Potential. Note: After proteolytic cleavage, CLAC is secreted By similarity.
Tissue specificity	Expressed predominantly in neurons with low levels also detected in heart, testis and eye. Ref.1
Post-translational modification	Undergoes proteolytic cleavage by furin protease to yield the soluble collagen-like Alzheimer amyloid plaque component By similarity. UniProtKB Q9BXS0 Glycosylated By similarity. UniProtKB Q9BXS0 Hydroxylated on proline and lysine residues By similarity. UniProtKB Q9BXS0
Sequence similarities	Contains 8 collagen-like domains.

Ontologies

Keywords
Cellular component	Amyloid Membrane
Domain	Collagen Repeat Signal-anchor Transmembrane Transmembrane helix
Ligand	Heparin-binding
PTM	Glycoprotein Hydroxylation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	collagen Inferred from electronic annotation. Source: UniProtKB-KW extracellular space Inferred from sequence or structural similarity. Source: UniProtKB integral to plasma membrane Inferred from sequence alignment Ref.1. Source: MGI
Molecular_function	beta-amyloid binding Inferred from sequence or structural similarity. Source: UniProtKB heparin binding Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 666	666	Collagen alpha-1(XXV) chain		PRO_0000259613
	Chain	113 – 654	542	Collagen-like Alzheimer amyloid plaque component By similarity UniProtKB Q9BXS0		PRO_0000259614
Regions
	Topological domain	1 – 33	33	Cytoplasmic Potential
	Transmembrane	34 – 54	21	Helical; Signal-anchor for type II membrane protein; Potential
	Topological domain	55 – 666	612	Extracellular Potential
	Domain	121 – 164	44	Collagen-like 1
	Domain	192 – 247	56	Collagen-like 2
	Domain	249 – 308	60	Collagen-like 3
	Domain	311 – 370	60	Collagen-like 4
	Domain	373 – 425	53	Collagen-like 5
	Domain	455 – 514	60	Collagen-like 6
	Domain	529 – 588	60	Collagen-like 7
	Domain	589 – 648	60	Collagen-like 8
	Region	181 – 188	8	Interaction with beta amyloid peptide By similarity UniProtKB Q9BXS0
Sites
	Site	112 – 113	2	Cleavage; by furin By similarity UniProtKB Q9BXS0
Experimental info
	Sequence conflict	64	1	A → V in AAK37475. Ref.1
	Sequence conflict	104	1	H → Y in AAK37475. Ref.1
	Sequence conflict	111	1	A → V in AAK37475. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q99MQ5 [UniParc]. Last modified October 3, 2012. Version 2. Checksum: DD8F5CAADE401366	FASTA	666	65,377
	10 20 30 40 50 60 MLVKKLAGKG GGRESGSEDP RPLGQRCAGT MPSCTALATL LSVVAVAFCF YLGVKTNDLQ 70 80 90 100 110 120 ARIAALESAK GTPSFHPLSD TVDELKAMVQ EKVERLLAQK SYEHMAKIRT AREAPLECNC 130 140 150 160 170 180 PAGPPGKRGK RGRRGESGPP GQPGPQGPPG PKGDKGEQGD QGPRMVFPKI NHGFLSADQQ 190 200 210 220 230 240 LIKRRLIKGD QGQAGPPGPP GPPGPRGPPG DTGKDGPRGM PGVPGEPGKP GEQGLMGPLG 250 260 270 280 290 300 PPGQKGSIGA PGTPGMDGQK GEPGSPGAAG QSGLPGPKGE PGKEGEKGDA GENGPKGDTG 310 320 330 340 350 360 EKGDPGSSAA GIKGEPGESG RPGQKGEPGL PGLPGLPGIK GEPGFIGPQG EPGLPGLPGT 370 380 390 400 410 420 KGDRGEAGPP GRGERGDPGA PGPKGKQGES GARGPKGSKG DRGDKGDSGA LGPRGPPGQK 430 440 450 460 470 480 GDPGATEIID YNGNLHEALQ RITTLTVTGP PGPPGPQGLQ GPKGEQGSPG IPGVDGEQGL 490 500 510 520 530 540 KGSKGDMGDP GVPGEKGGLG LPGLPGANGV KGEKGDTGLP GPQGPSIIGP PGPPGPHGPP 550 560 570 580 590 600 GPMGPHGLPG PKGASGLDGK PGSRGADGPI GPHGPAGPKG ERGEKGAMGE PGPRGPYGLP 610 620 630 640 650 660 GKDGEPGLDG FPGPRGEKGD LGEKGEKGFR GVKGEKGEPG QPGLDGLDAP CQLGPDGLPM PGCWQK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"CLAC: a novel Alzheimer amyloid plaque component derived from a transmembrane precursor, CLAC-P/collagen type XXV." Hashimoto T., Wakabayashi T., Watanabe A., Kowa H., Hosoda R., Nakamura A., Kanazawa I., Arai T., Takio K., Mann D.M.A., Iwatsubo T. EMBO J. 21:1524-1534(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY. Strain: BALB/c. Tissue: Brain.
[2]	"Lineage-specific biology revealed by a finished genome assembly of the mouse." Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P. PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C57BL/6J.
[3]	Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF315290 mRNA. Translation: AAK37475.1. AC091312 Genomic DNA. No translation available. AC110540 Genomic DNA. No translation available. AC114642 Genomic DNA. No translation available. AC164078 Genomic DNA. No translation available. CH466532 Genomic DNA. Translation: EDL12220.1.
IPI	IPI00117959.
RefSeq	NP_084114.2. NM_029838.4.
UniGene	Mm.91728.
3D structure databases
ProteinModelPortal	Q99MQ5.
SMR	Q99MQ5. Positions 547-581, 625-661.
ModBase	Search...
PTM databases
PhosphoSite	Q99MQ5.
Proteomic databases
PRIDE	Q99MQ5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000080335; ENSMUSP00000079210; ENSMUSG00000058897.
GeneID	77018.
KEGG	mmu:77018.
UCSC	uc008riw.1. mouse.
Organism-specific databases
CTD	84570.
MGI	MGI:1924268. Col25a1.
Phylogenomic databases
eggNOG	NOG12793.
GeneTree	ENSGT00530000063274.
HOGENOM	HOG000085653.
HOVERGEN	HBG101380.
InParanoid	Q99MQ5.
OMA	GPKGSKX.
Gene expression databases
CleanEx	MM_COL25A1.
Genevestigator	Q99MQ5.
Family and domain databases
InterPro	IPR008160. Collagen. [Graphical view]
Pfam	PF01391. Collagen. 6 hits. [Graphical view]
ProtoNet	Search...
Other
NextBio	13888081.
SOURCE	Search...

Entry information

Entry name

COPA1_MOUSE

Accession

Primary (citable) accession number: Q99MQ5
Secondary accession number(s): G3X9H6

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 31, 2006
Last sequence update:	October 3, 2012
Last modified:	May 1, 2013
This is version 71 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program