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Q99MQ5

- COPA1_MOUSE

UniProt

Q99MQ5 - COPA1_MOUSE

Protein

Collagen alpha-1(XXV) chain

Gene

Col25a1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 83 (01 Oct 2014)
      Sequence version 2 (03 Oct 2012)
      Previous versions | rss
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    Functioni

    Inhibits fibrillization of beta amyloid peptide during the elongation phase. Has also been shown to assemble amyloid fibrils into protease-resistant aggregates. Binds heparin By similarity.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei112 – 1132Cleavage; by furinBy similarity

    GO - Molecular functioni

    1. beta-amyloid binding Source: UniProtKB
    2. heparin binding Source: UniProtKB

    Keywords - Ligandi

    Heparin-binding

    Enzyme and pathway databases

    ReactomeiREACT_198984. Collagen biosynthesis and modifying enzymes.
    REACT_199055. Collagen degradation.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Collagen alpha-1(XXV) chain
    Alternative name(s):
    CLAC-P
    Cleaved into the following chain:
    Gene namesi
    Name:Col25a1Imported
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 3

    Organism-specific databases

    MGIiMGI:1924268. Col25a1.

    Subcellular locationi

    Membrane Curated; Single-pass type II membrane protein Curated
    Note: After proteolytic cleavage, CLAC is secreted.By similarity

    GO - Cellular componenti

    1. collagen trimer Source: UniProtKB-KW
    2. extracellular region Source: MGI
    3. extracellular space Source: UniProtKB
    4. integral component of plasma membrane Source: MGI

    Keywords - Cellular componenti

    Amyloid, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 666666Collagen alpha-1(XXV) chainPRO_0000259613Add
    BLAST
    Chaini113 – 654542Collagen-like Alzheimer amyloid plaque componentBy similarityPRO_0000259614Add
    BLAST

    Post-translational modificationi

    Undergoes proteolytic cleavage by furin protease to yield the soluble collagen-like Alzheimer amyloid plaque component.By similarity
    Glycosylated.By similarity
    Hydroxylated on proline and lysine residues.By similarity

    Keywords - PTMi

    Glycoprotein, Hydroxylation

    Proteomic databases

    PRIDEiQ99MQ5.

    PTM databases

    PhosphoSiteiQ99MQ5.

    Expressioni

    Tissue specificityi

    Expressed predominantly in neurons with low levels also detected in heart, testis and eye.1 Publication

    Gene expression databases

    CleanExiMM_COL25A1.
    GenevestigatoriQ99MQ5.

    Interactioni

    Subunit structurei

    Forms homodimers and homotrimers. Binds to the fibrillized forms of beta amyloid peptide 40 (beta-APP40) and beta amyloid peptide 42 (beta-APP42). Found associated with beta-APP42 more frequently than with beta-APP40 By similarity.By similarity

    Structurei

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 3333CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini55 – 666612ExtracellularSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei34 – 5421Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini121 – 16444Collagen-like 1Sequence AnalysisAdd
    BLAST
    Domaini192 – 24756Collagen-like 2Sequence AnalysisAdd
    BLAST
    Domaini249 – 30860Collagen-like 3Sequence AnalysisAdd
    BLAST
    Domaini311 – 37060Collagen-like 4Sequence AnalysisAdd
    BLAST
    Domaini373 – 42553Collagen-like 5Sequence AnalysisAdd
    BLAST
    Domaini455 – 51460Collagen-like 6Sequence AnalysisAdd
    BLAST
    Domaini529 – 58860Collagen-like 7Sequence AnalysisAdd
    BLAST
    Domaini589 – 64860Collagen-like 8Sequence AnalysisAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni181 – 1888Interaction with beta amyloid peptideBy similarity

    Sequence similaritiesi

    Contains 8 collagen-like domains.Sequence Analysis

    Keywords - Domaini

    Collagen, Repeat, Signal-anchor, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG12793.
    GeneTreeiENSGT00750000117303.
    HOGENOMiHOG000085653.
    HOVERGENiHBG101380.
    InParanoidiQ99MQ5.
    OMAiGRRGESX.
    OrthoDBiEOG7D59P1.
    TreeFamiTF338175.

    Family and domain databases

    InterProiIPR029575. CLAC-P.
    IPR008160. Collagen.
    [Graphical view]
    PANTHERiPTHR24023:SF412. PTHR24023:SF412. 1 hit.
    PfamiPF01391. Collagen. 6 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q99MQ5-1 [UniParc]FASTAAdd to Basket

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    MLVKKLAGKG GGRESGSEDP RPLGQRCAGT MPSCTALATL LSVVAVAFCF    50
    YLGVKTNDLQ ARIAALESAK GTPSFHPLSD TVDELKAMVQ EKVERLLAQK 100
    SYEHMAKIRT AREAPLECNC PAGPPGKRGK RGRRGESGPP GQPGPQGPPG 150
    PKGDKGEQGD QGPRMVFPKI NHGFLSADQQ LIKRRLIKGD QGQAGPPGPP 200
    GPPGPRGPPG DTGKDGPRGM PGVPGEPGKP GEQGLMGPLG PPGQKGSIGA 250
    PGTPGMDGQK GEPGSPGAAG QSGLPGPKGE PGKEGEKGDA GENGPKGDTG 300
    EKGDPGSSAA GIKGEPGESG RPGQKGEPGL PGLPGLPGIK GEPGFIGPQG 350
    EPGLPGLPGT KGDRGEAGPP GRGERGDPGA PGPKGKQGES GARGPKGSKG 400
    DRGDKGDSGA LGPRGPPGQK GDPGATEIID YNGNLHEALQ RITTLTVTGP 450
    PGPPGPQGLQ GPKGEQGSPG IPGVDGEQGL KGSKGDMGDP GVPGEKGGLG 500
    LPGLPGANGV KGEKGDTGLP GPQGPSIIGP PGPPGPHGPP GPMGPHGLPG 550
    PKGASGLDGK PGSRGADGPI GPHGPAGPKG ERGEKGAMGE PGPRGPYGLP 600
    GKDGEPGLDG FPGPRGEKGD LGEKGEKGFR GVKGEKGEPG QPGLDGLDAP 650
    CQLGPDGLPM PGCWQK 666
    Length:666
    Mass (Da):65,377
    Last modified:October 3, 2012 - v2
    Checksum:iDD8F5CAADE401366
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti64 – 641A → V in AAK37475. (PubMed:11927537)Curated
    Sequence conflicti104 – 1041H → Y in AAK37475. (PubMed:11927537)Curated
    Sequence conflicti111 – 1111A → V in AAK37475. (PubMed:11927537)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF315290 mRNA. Translation: AAK37475.1.
    AC091312 Genomic DNA. No translation available.
    AC110540 Genomic DNA. No translation available.
    AC114642 Genomic DNA. No translation available.
    AC164078 Genomic DNA. No translation available.
    CH466532 Genomic DNA. Translation: EDL12220.1.
    CCDSiCCDS38637.1.
    RefSeqiNP_084114.2. NM_029838.4.
    UniGeneiMm.91728.

    Genome annotation databases

    EnsembliENSMUST00000080335; ENSMUSP00000079210; ENSMUSG00000058897.
    GeneIDi77018.
    KEGGimmu:77018.
    UCSCiuc008riw.2. mouse.
    uc008riz.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF315290 mRNA. Translation: AAK37475.1 .
    AC091312 Genomic DNA. No translation available.
    AC110540 Genomic DNA. No translation available.
    AC114642 Genomic DNA. No translation available.
    AC164078 Genomic DNA. No translation available.
    CH466532 Genomic DNA. Translation: EDL12220.1 .
    CCDSi CCDS38637.1.
    RefSeqi NP_084114.2. NM_029838.4.
    UniGenei Mm.91728.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    PTM databases

    PhosphoSitei Q99MQ5.

    Proteomic databases

    PRIDEi Q99MQ5.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000080335 ; ENSMUSP00000079210 ; ENSMUSG00000058897 .
    GeneIDi 77018.
    KEGGi mmu:77018.
    UCSCi uc008riw.2. mouse.
    uc008riz.1. mouse.

    Organism-specific databases

    CTDi 84570.
    MGIi MGI:1924268. Col25a1.

    Phylogenomic databases

    eggNOGi NOG12793.
    GeneTreei ENSGT00750000117303.
    HOGENOMi HOG000085653.
    HOVERGENi HBG101380.
    InParanoidi Q99MQ5.
    OMAi GRRGESX.
    OrthoDBi EOG7D59P1.
    TreeFami TF338175.

    Enzyme and pathway databases

    Reactomei REACT_198984. Collagen biosynthesis and modifying enzymes.
    REACT_199055. Collagen degradation.

    Miscellaneous databases

    NextBioi 13888081.
    PROi Q99MQ5.
    SOURCEi Search...

    Gene expression databases

    CleanExi MM_COL25A1.
    Genevestigatori Q99MQ5.

    Family and domain databases

    InterProi IPR029575. CLAC-P.
    IPR008160. Collagen.
    [Graphical view ]
    PANTHERi PTHR24023:SF412. PTHR24023:SF412. 1 hit.
    Pfami PF01391. Collagen. 6 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "CLAC: a novel Alzheimer amyloid plaque component derived from a transmembrane precursor, CLAC-P/collagen type XXV."
      Hashimoto T., Wakabayashi T., Watanabe A., Kowa H., Hosoda R., Nakamura A., Kanazawa I., Arai T., Takio K., Mann D.M.A., Iwatsubo T.
      EMBO J. 21:1524-1534(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
      Strain: BALB/cImported.
      Tissue: BrainImported.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    3. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
      Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

    Entry informationi

    Entry nameiCOPA1_MOUSE
    AccessioniPrimary (citable) accession number: Q99MQ5
    Secondary accession number(s): G3X9H6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 31, 2006
    Last sequence update: October 3, 2012
    Last modified: October 1, 2014
    This is version 83 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3