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Protein

Serine/threonine-protein kinase PINK1, mitochondrial

Gene

Pink1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Protects against mitochondrial dysfunction during cellular stress by phosphorylating mitochondrial proteins. Involved in the clearance of damaged mitochondria via selective autophagy (mitophagy) by mediating activation and translocation of PARK2. Targets PARK2 to dysfunctional depolarized mitochondria through the phosphorylation of MFN2. Activates PARK2 in 2 steps: (1) by mediating phosphorylation at 'Ser-65' of PARK2 and (2) mediating phosphorylation of ubiquitin, converting PARK2 to its fully-active form (PubMed:24784582).By similarity2 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.By similarity

Cofactori

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei186ATPPROSITE-ProRule annotation1
Active sitei361Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi162 – 170ATPPROSITE-ProRule annotationBy similarity9

GO - Molecular functioni

  • ATP binding Source: UniProtKB
  • C3HC4-type RING finger domain binding Source: MGI
  • calcium-dependent protein kinase activity Source: MGI
  • kinase activity Source: MGI
  • magnesium ion binding Source: UniProtKB
  • protease binding Source: MGI
  • protein kinase activity Source: MGI
  • protein kinase B binding Source: MGI
  • protein serine/threonine kinase activity Source: UniProtKB
  • ubiquitin protein ligase binding Source: MGI

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Autophagy

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-5205685. Pink/Parkin Mediated Mitophagy.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein kinase PINK1, mitochondrial (EC:2.7.11.1)
Alternative name(s):
BRPK
PTEN-induced putative kinase protein 1
Gene namesi
Name:Pink1
OrganismiMus musculus (Mouse)Imported
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:1916193. Pink1.

Subcellular locationi

  • Mitochondrion outer membrane By similarity; Single-pass membrane protein By similarity
  • Cytoplasmcytosol By similarity

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini78 – 93Mitochondrial intermembraneSequence analysisAdd BLAST16
Transmembranei94 – 110HelicalSequence analysisAdd BLAST17
Topological domaini111 – 580CytoplasmicSequence analysisAdd BLAST470

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Membrane, Mitochondrion, Mitochondrion outer membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 77MitochondrionSequence analysisAdd BLAST77
ChainiPRO_000002437078 – 580Serine/threonine-protein kinase PINK1, mitochondrialAdd BLAST503

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei227PhosphoserineBy similarity1
Modified residuei401PhosphoserineBy similarity1

Post-translational modificationi

Autophosphorylated.By similarity
Two shorter forms of 55 kDa and 48 kDa seem to be produced by proteolytic cleavage and localize mainly in cytosol.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ99MQ3.
PRIDEiQ99MQ3.

PTM databases

iPTMnetiQ99MQ3.
PhosphoSitePlusiQ99MQ3.

Expressioni

Tissue specificityi

High levels expressed in testis, lower levels in brain, heart, lung, liver and kidney.1 Publication

Gene expression databases

BgeeiENSMUSG00000028756.
CleanExiMM_PINK1.
ExpressionAtlasiQ99MQ3. baseline and differential.
GenevisibleiQ99MQ3. MM.

Interactioni

Subunit structurei

Interacts with PARK2 and FBXO7. Forms a complex with PARK2 and PARK7.By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi213129. 2 interactors.
IntActiQ99MQ3. 1 interactor.
STRINGi10090.ENSMUSP00000030536.

Structurei

3D structure databases

ProteinModelPortaliQ99MQ3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini156 – 510Protein kinasePROSITE-ProRule annotationCuratedAdd BLAST355

Sequence similaritiesi

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Keywords - Domaini

Transit peptide, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG4158. Eukaryota.
ENOG410YE6P. LUCA.
GeneTreeiENSGT00390000001206.
HOVERGENiHBG053601.
InParanoidiQ99MQ3.
KOiK05688.
OMAiGPKQLAP.
OrthoDBiEOG091G03V6.
PhylomeDBiQ99MQ3.
TreeFamiTF313183.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 3 hits.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q99MQ3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAVRQALGRG LQLGRALLLR FAPKPGPLFG WGKPGPAAAW GRGERPGQVV
60 70 80 90 100
SPGAQPRPVG LPLPDRYRFF RQSVAGLAAR IQRQFMVRAR GGAGPCGRAV
110 120 130 140 150
FLAFGLGLGL IEEKQAEGRR AASACQEIQA IFTQKTKRVS DPLDTRCWQG
160 170 180 190 200
FRLEDYLIGQ AIGKGCNAAV YEATMPTLPQ HLEKAKHLGL IGKGPDVVLK
210 220 230 240 250
GADGEQAPGT PTFPFAIKMM WNISAGSSSE AILSKMSQEL VPASRVALAG
260 270 280 290 300
EYGAVTYRRS RDGPKQLAPH PNIIRVFRAF TSSVPLLPGA LADYPDMLPP
310 320 330 340 350
HYYPEGLGHG RTLFLVMKNY PCTLRQYLEE QTPSSRLATM MTLQLLEGVD
360 370 380 390 400
HLVQQGIAHR DLKSDNILVE WDSDGCPWLV ISDFGCCLAD QHVGLRLPFN
410 420 430 440 450
SSSVERGGNG SLMAPEVSTA HSGPSAVIDY SKADTWAVGA IAYEIFGLAN
460 470 480 490 500
PFYGQGSAHL ESRSYQEAQL PEMPESVPPE ARRLVRSLLQ REASKRPSAR
510 520 530 540 550
LAANVLHLSL WGEHLLALKN LKLDKMIAWL LQQSAATLLA DRLREKSCVE
560 570 580
TKLQMLFLAN LECEALCQAA LLLSSWRAAP
Length:580
Mass (Da):63,181
Last modified:June 7, 2004 - v2
Checksum:i8AB6994B8B4E443E
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti9R → P in BAB55651 (PubMed:11494141).Curated1
Sequence conflicti48Q → R in AAK28061 (PubMed:14607334).Curated1
Sequence conflicti249A → D in AAK28061 (PubMed:14607334).Curated1
Sequence conflicti425S → G in AAH54349 (PubMed:15489334).Curated1
Sequence conflicti475E → K in AAK28061 (PubMed:14607334).Curated1
Sequence conflicti476S → L in AAH54349 (PubMed:15489334).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB053476 mRNA. Translation: BAB55651.1.
AF316872 mRNA. Translation: AAK28061.1.
AL807249 Genomic DNA. Translation: CAM18873.1.
BC044743 mRNA. Translation: AAH44743.1.
BC054349 mRNA. Translation: AAH54349.1.
BC067066 mRNA. Translation: AAH67066.1.
CCDSiCCDS18825.1.
RefSeqiNP_081156.2. NM_026880.2.
UniGeneiMm.18539.

Genome annotation databases

EnsembliENSMUST00000030536; ENSMUSP00000030536; ENSMUSG00000028756.
GeneIDi68943.
KEGGimmu:68943.
UCSCiuc008vku.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB053476 mRNA. Translation: BAB55651.1.
AF316872 mRNA. Translation: AAK28061.1.
AL807249 Genomic DNA. Translation: CAM18873.1.
BC044743 mRNA. Translation: AAH44743.1.
BC054349 mRNA. Translation: AAH54349.1.
BC067066 mRNA. Translation: AAH67066.1.
CCDSiCCDS18825.1.
RefSeqiNP_081156.2. NM_026880.2.
UniGeneiMm.18539.

3D structure databases

ProteinModelPortaliQ99MQ3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi213129. 2 interactors.
IntActiQ99MQ3. 1 interactor.
STRINGi10090.ENSMUSP00000030536.

PTM databases

iPTMnetiQ99MQ3.
PhosphoSitePlusiQ99MQ3.

Proteomic databases

PaxDbiQ99MQ3.
PRIDEiQ99MQ3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000030536; ENSMUSP00000030536; ENSMUSG00000028756.
GeneIDi68943.
KEGGimmu:68943.
UCSCiuc008vku.1. mouse.

Organism-specific databases

CTDi65018.
MGIiMGI:1916193. Pink1.

Phylogenomic databases

eggNOGiKOG4158. Eukaryota.
ENOG410YE6P. LUCA.
GeneTreeiENSGT00390000001206.
HOVERGENiHBG053601.
InParanoidiQ99MQ3.
KOiK05688.
OMAiGPKQLAP.
OrthoDBiEOG091G03V6.
PhylomeDBiQ99MQ3.
TreeFamiTF313183.

Enzyme and pathway databases

ReactomeiR-MMU-5205685. Pink/Parkin Mediated Mitophagy.

Miscellaneous databases

ChiTaRSiPink1. mouse.
PROiQ99MQ3.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000028756.
CleanExiMM_PINK1.
ExpressionAtlasiQ99MQ3. baseline and differential.
GenevisibleiQ99MQ3. MM.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 3 hits.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPINK1_MOUSE
AccessioniPrimary (citable) accession number: Q99MQ3
Secondary accession number(s): A2AM77
, Q7TMZ3, Q811I8, Q91XU5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 7, 2004
Last sequence update: June 7, 2004
Last modified: November 30, 2016
This is version 130 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.