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Protein

BRCA1-associated protein

Gene

Brap

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Negatively regulates MAP kinase activation by limiting the formation of Raf/MEK complexes probably by inactivation of the KSR1 scaffold protein. Also acts as a Ras responsive E3 ubiquitin ligase that, on activation of Ras, is modified by auto-polyubiquitination resulting in the release of inhibition of Raf/MEK complex formation. May also act as a cytoplasmic retention protein with a role in regulating nuclear transport (By similarity).By similarity

Catalytic activityi

S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N6-ubiquitinyl-[acceptor protein]-L-lysine.By similarity

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri263 – 303RING-typeCuratedAdd BLAST41
Zinc fingeri314 – 375UBP-typeAdd BLAST62

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processUbl conjugation pathway
LigandMetal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-MMU-5673000. RAF activation.
R-MMU-5675221. Negative regulation of MAPK pathway.
UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
BRCA1-associated protein (EC:2.3.2.27By similarity)
Alternative name(s):
BRAP2
Impedes mitogenic signal propagation
Short name:
IMP
RING-type E3 ubiquitin transferase BRAP2Curated
Gene namesi
Name:BrapImported
OrganismiMus musculus (Mouse)Imported
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:1919649. Brap.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000558261 – 591BRCA1-associated proteinAdd BLAST591

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei52PhosphoserineBy similarity1
Modified residuei116PhosphoserineBy similarity1
Modified residuei118PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ99MP8.
PeptideAtlasiQ99MP8.
PRIDEiQ99MP8.

PTM databases

iPTMnetiQ99MP8.
PhosphoSitePlusiQ99MP8.

Expressioni

Tissue specificityi

Isoform 2 is highly expressed in testis, lower levels in brain, heart, lung, stomach, colon, uterus, liver and kidney. Isoform 1 is only expressed in the testis. Isoform 2 is predominant over isoform 1 in both fetal and adult testis.1 Publication

Gene expression databases

BgeeiENSMUSG00000029458.
CleanExiMM_BRAP.
ExpressionAtlasiQ99MP8. baseline and differential.
GenevisibleiQ99MP8. MM.

Interactioni

Subunit structurei

Interacts with the nuclear localization signal of BRCA1 and with the N-terminal of KSR1. The C-terminal portion of BRCA1 interacts with DDB1.By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
Akap3O889873EBI-10818333,EBI-9033539

GO - Molecular functioni

Protein-protein interaction databases

BioGridi215356. 12 interactors.
CORUMiQ99MP8.
IntActiQ99MP8. 3 interactors.
STRINGi10090.ENSMUSP00000031414.

Structurei

3D structure databases

ProteinModelPortaliQ99MP8.
SMRiQ99MP8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili430 – 536Sequence analysisAdd BLAST107

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri263 – 303RING-typeCuratedAdd BLAST41
Zinc fingeri314 – 375UBP-typeAdd BLAST62

Keywords - Domaini

Coiled coil, Zinc-finger

Phylogenomic databases

eggNOGiKOG0804. Eukaryota.
ENOG410XSS2. LUCA.
GeneTreeiENSGT00500000044909.
HOGENOMiHOG000190616.
HOVERGENiHBG050729.
InParanoidiQ99MP8.
KOiK10632.
OMAiNQYMALI.
OrthoDBiEOG091G07BQ.
PhylomeDBiQ99MP8.
TreeFamiTF313622.

Family and domain databases

CDDicd12718. RRM_BRAP2. 1 hit.
Gene3Di3.30.40.10. 2 hits.
InterProiView protein in InterPro
IPR011422. BRAP2.
IPR034932. BRAP2_RRM.
IPR035979. RBD_domain_sf.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
IPR001607. Znf_UBP.
PfamiView protein in Pfam
PF07576. BRAP2. 1 hit.
PF13639. zf-RING_2. 1 hit.
PF02148. zf-UBP. 1 hit.
ProDomiView protein in ProDom or Entries sharing at least one domain
PD017029. BRAP2. 1 hit.
SMARTiView protein in SMART
SM00184. RING. 1 hit.
SM00290. ZnF_UBP. 1 hit.
SUPFAMiSSF54928. SSF54928. 1 hit.
PROSITEiView protein in PROSITE
PS50089. ZF_RING_2. 1 hit.
PS50271. ZF_UBP. 1 hit.

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 11 Publication (identifier: Q99MP8-1) [UniParc]FASTAAdd to basket
Also known as: alpha1 Publication

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSVSLVVIRL ELAGHSPVPT DFGFSAAAGE MSDEEIKKKT LASAVACLEG
60 70 80 90 100
KSAGEKAAII HQHLGRREMT DVIIETMKAR ADEVRDTVEE KKPSAAPVSA
110 120 130 140 150
QRSREQSESV NTAPESPSKQ LPDQISFFSG NPSVEIVHGI MHLYKTNKMT
160 170 180 190 200
SLKEDVRRSA MLCVLTVPAT MTSHDLMKFV APFNDVIEQM KIIRDSTPNQ
210 220 230 240 250
YMVLIKFSAQ ADADSFYMAC NGRQFNSIED DVCQLVYVER AEVLKSEDGA
260 270 280 290 300
SLPVMDLTEL PKCTVCLERM DESVNGILTT LCNHSFHSQC LQRWDDTTCP
310 320 330 340 350
VCRYCQTPEP VEENKCFECG VQENLWICLI CGHIGCGRYV SRHAYKHFEE
360 370 380 390 400
TQHTYAMQLT NHRVWDYAGD NYVHRLVASK TDGKIVQYEC EGDTCQEEKI
410 420 430 440 450
DALQLEYSYL LTSQLESQRI YWENKIVRIE KDTAEEINNM KTKFKETIEK
460 470 480 490 500
CDSLELRLSD LLKEKQSVER KCTQLNTRVA KLSTELQEEQ ELNKCLRANQ
510 520 530 540 550
LVLQNQLKEE EKLLKETCAQ KDLQITEIQE QLRDVMFYLE TQQQISHLPA
560 570 580 590
ETRQEIQEGQ INIAMASAPN PPSSGAGGKL QSRKGRSKRG K
Length:591
Mass (Da):66,991
Last modified:June 1, 2001 - v1
Checksum:iDF925A28C0388E72
GO
Isoform 21 Publication (identifier: Q99MP8-2) [UniParc]FASTAAdd to basket
Also known as: beta1 Publication

The sequence of this isoform differs from the canonical sequence as follows:
     1-30: Missing.

Show »
Length:561
Mass (Da):63,951
Checksum:i54DBA485E084D520
GO
Isoform 3Curated (identifier: Q99MP8-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-217: Missing.

Note: No experimental confirmation available.Curated
Show »
Length:374
Mass (Da):43,165
Checksum:iBC8C4F2F1E3EE012
GO

Sequence cautioni

The sequence BAB29036 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti111N → I (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0507621 – 217Missing in isoform 3. 1 PublicationAdd BLAST217
Alternative sequenceiVSP_0507611 – 30Missing in isoform 2. 1 PublicationAdd BLAST30

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF321920 mRNA. Translation: AAK28079.1.
AF321921 mRNA. Translation: AAK28080.1.
BC038490 mRNA. Translation: AAH38490.1.
AK013885 mRNA. Translation: BAB29036.1. Different initiation.
AK034212 mRNA. Translation: BAC28633.1.
CCDSiCCDS19640.1. [Q99MP8-1]
CCDS80389.1. [Q99MP8-2]
RefSeqiNP_001276473.1. NM_001289544.1. [Q99MP8-2]
NP_082503.2. NM_028227.3. [Q99MP8-1]
XP_006530532.1. XM_006530469.1.
UniGeneiMm.153372.

Genome annotation databases

EnsembliENSMUST00000031414; ENSMUSP00000031414; ENSMUSG00000029458. [Q99MP8-1]
ENSMUST00000111765; ENSMUSP00000107395; ENSMUSG00000029458. [Q99MP8-2]
GeneIDi72399.
KEGGimmu:72399.
UCSCiuc008zkc.2. mouse. [Q99MP8-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiBRAP_MOUSE
AccessioniPrimary (citable) accession number: Q99MP8
Secondary accession number(s): Q8CC00
, Q8CHX1, Q99MP7, Q9CXX8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 19, 2004
Last sequence update: June 1, 2001
Last modified: October 25, 2017
This is version 134 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways