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Protein

Prenylcysteine oxidase

Gene

Pcyox1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the degradation of prenylated proteins. Cleaves the thioether bond of prenyl-L-cysteines, such as farnesylcysteine and geranylgeranylcysteine (By similarity).By similarity

Catalytic activityi

An S-prenyl-L-cysteine + O2 + H2O = a prenal + L-cysteine + H2O2.

Cofactori

FADBy similarity

GO - Molecular functioni

  1. chloride-transporting ATPase activity Source: RGD
  2. prenylcysteine oxidase activity Source: UniProtKB-EC

GO - Biological processi

  1. chloride transmembrane transport Source: GOC
  2. chloride transport Source: RGD
  3. prenylated protein catabolic process Source: Ensembl
  4. prenylcysteine catabolic process Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

FAD, Flavoprotein

Enzyme and pathway databases

BRENDAi3.6.3.11. 5301.

Names & Taxonomyi

Protein namesi
Recommended name:
Prenylcysteine oxidase (EC:1.8.3.5)
Alternative name(s):
Chloride ion pump-associated 55 kDa protein
Gene namesi
Name:Pcyox1
Synonyms:Clp55
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 4

Organism-specific databases

RGDi628652. Pcyox1.

Subcellular locationi

Lysosome By similarity

GO - Cellular componenti

  1. extracellular vesicular exosome Source: Ensembl
  2. lysosome Source: UniProtKB-SubCell
  3. plasma membrane Source: RGD
  4. very-low-density lipoprotein particle Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Lysosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2828Sequence AnalysisAdd
BLAST
Chaini29 – 504476Prenylcysteine oxidasePRO_0000023302Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Cross-linki162 – 162Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
Glycosylationi196 – 1961N-linked (GlcNAc...)Sequence Analysis
Glycosylationi322 – 3221N-linked (GlcNAc...)Sequence Analysis
Glycosylationi352 – 3521N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein, Isopeptide bond, Ubl conjugation

Proteomic databases

PaxDbiQ99ML5.
PRIDEiQ99ML5.

Expressioni

Tissue specificityi

Expressed mainly in cerebrum.1 Publication

Gene expression databases

GenevestigatoriQ99ML5.

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000022532.

Structurei

3D structure databases

ProteinModelPortaliQ99ML5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the prenylcysteine oxidase family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG73316.
GeneTreeiENSGT00390000011206.
HOGENOMiHOG000241149.
HOVERGENiHBG053532.
InParanoidiQ99ML5.
KOiK05906.
OMAiNGIECAA.
OrthoDBiEOG7VB2F5.
PhylomeDBiQ99ML5.
TreeFamiTF329001.

Family and domain databases

InterProiIPR010795. Prenylcys_lyase.
IPR017046. Prenylcysteine_Oxase.
[Graphical view]
PfamiPF07156. Prenylcys_lyase. 1 hit.
[Graphical view]
PIRSFiPIRSF036292. Prenylcysteine_oxidase. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q99ML5-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MGRFAATLVG SLFGLGLLLC GLGRLASAEP RAPPEKIAIV GAGIGGTSSA
60 70 80 90 100
YYLRKKFGKD VKIDVFEREE IGGRLATLKV QGHDYEAGGS VIHPLNLHMK
110 120 130 140 150
RFVKELGLSS VPASGGLVGV YNGKSLVFEE SSWFIINVIK LVWRYGFQSL
160 170 180 190 200
RMHMWVEDLL DKFMRIYRYQ SHDYAFSSVE KLMYAIGGDD YVRLLNQTLR
210 220 230 240 250
ENLKKAGFSE TFLNEMIAPV MKVNFGQSTD LNAFVGAVSM TAADSNLWAV
260 270 280 290 300
EGGNKVVCSG LLQASNSNLI SGSVMSIEKT RTKQTGTPPK MYEVVYKTGS
310 320 330 340 350
EIHSDFYDIV LVAAPLNRKM SNITFRNFDP PIEEFNDPYQ QLVTTLIKGE
360 370 380 390 400
LNSTLFSSRH KDQFGLSAIL VTDDSDMFIN SLSIVASVSH KEGPPPAVDG
410 420 430 440 450
MHVWKTFSKD ILTKEQISKL FLSYDYAVRK PWLSYPYYNP PQKCPSIILH
460 470 480 490 500
DRLYYLNGIE FAASCMEMSA IAGYNAALLA YHRWNGNEDM IDQDDLYEKL

KTEL
Length:504
Mass (Da):56,288
Last modified:June 1, 2001 - v1
Checksum:iD2778026128FE9D4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF332142 mRNA. Translation: AAK16548.1.
BC078719 mRNA. Translation: AAH78719.1.
PIRiJC7782.
RefSeqiNP_659553.1. NM_145085.2.
UniGeneiRn.34505.

Genome annotation databases

EnsembliENSRNOT00000022532; ENSRNOP00000022532; ENSRNOG00000016704.
GeneIDi246302.
KEGGirno:246302.
UCSCiRGD:628652. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF332142 mRNA. Translation: AAK16548.1.
BC078719 mRNA. Translation: AAH78719.1.
PIRiJC7782.
RefSeqiNP_659553.1. NM_145085.2.
UniGeneiRn.34505.

3D structure databases

ProteinModelPortaliQ99ML5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000022532.

Proteomic databases

PaxDbiQ99ML5.
PRIDEiQ99ML5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000022532; ENSRNOP00000022532; ENSRNOG00000016704.
GeneIDi246302.
KEGGirno:246302.
UCSCiRGD:628652. rat.

Organism-specific databases

CTDi51449.
RGDi628652. Pcyox1.

Phylogenomic databases

eggNOGiNOG73316.
GeneTreeiENSGT00390000011206.
HOGENOMiHOG000241149.
HOVERGENiHBG053532.
InParanoidiQ99ML5.
KOiK05906.
OMAiNGIECAA.
OrthoDBiEOG7VB2F5.
PhylomeDBiQ99ML5.
TreeFamiTF329001.

Enzyme and pathway databases

BRENDAi3.6.3.11. 5301.

Miscellaneous databases

NextBioi623706.
PROiQ99ML5.

Gene expression databases

GenevestigatoriQ99ML5.

Family and domain databases

InterProiIPR010795. Prenylcys_lyase.
IPR017046. Prenylcysteine_Oxase.
[Graphical view]
PfamiPF07156. Prenylcys_lyase. 1 hit.
[Graphical view]
PIRSFiPIRSF036292. Prenylcysteine_oxidase. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning and characterization of the Cl(-) pump-associated 55-kDa protein in rat brain."
    Kitagawa K., Yagyu K., Yamamoto A., Hattori N., Omori K., Zeng X.-T., Inagaki C.
    Biochem. Biophys. Res. Commun. 289:363-371(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
    Strain: Wistar.
    Tissue: Brain.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Kidney.
  3. Lubec G., Kang S.U., Lubec S.
    Submitted (SEP-2007) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 291-318 AND 420-429, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: Sprague-Dawley.
    Tissue: Brain.

Entry informationi

Entry nameiPCYOX_RAT
AccessioniPrimary (citable) accession number: Q99ML5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 29, 2005
Last sequence update: June 1, 2001
Last modified: January 7, 2015
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.