Reviewed, UniProtKB/Swiss-Prot Q99ML5 (PCYOX_RAT)

Names and origin

Protein names

Recommended name:
    Prenylcysteine oxidase
    EC=1.8.3.5
Alternative name(s):
    Chloride ion pump-associated 55 kDa protein

Gene names

Name:	Pcyox1
Synonyms:	Clp55

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	504 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Involved in the degradation of prenylated proteins. Cleaves the thioether bond of prenyl-L-cysteines, such as farnesylcysteine and geranylgeranylcysteine By similarity.
Catalytic activity	An S-prenyl-L-cysteine + O2 + H2O = a prenal + L-cysteine + H2O2.
Cofactor	FAD By similarity.
Subcellular location	Lysosome By similarity.
Tissue specificity	Expressed mainly in cerebrum. Ref.1
Sequence similarities	Belongs to the prenylcysteine oxidase family.

Ontologies

Keywords
Cellular component	Lysosome
Domain	Signal
Ligand	FAD
Molecular function	Oxidoreductase
PTM	Glycoprotein Isopeptide bond Ubl conjugation
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	chloride transport Ref.1 Inferred from mutant phenotype. Source: RGD oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	lysosome Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Ref.1 Inferred from direct assay. Source: RGD
Molecular function	chloride-transporting ATPase activity Ref.1 Inferred from mutant phenotype. Source: RGD prenylcysteine oxidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 28	28	Potential
	Chain	29 – 504	476	Prenylcysteine oxidase		PRO_0000023302
Amino acid modifications
	Glycosylation	196	1	N-linked (GlcNAc...) Potential
	Glycosylation	322	1	N-linked (GlcNAc...) Potential
	Glycosylation	352	1	N-linked (GlcNAc...) Potential
	Cross-link	162		Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q99ML5-1 [UniParc]. Last modified June 1, 2001. Version 1. Checksum: D2778026128FE9D4	FASTA	504	56,288
	10 20 30 40 50 60 MGRFAATLVG SLFGLGLLLC GLGRLASAEP RAPPEKIAIV GAGIGGTSSA YYLRKKFGKD 70 80 90 100 110 120 VKIDVFEREE IGGRLATLKV QGHDYEAGGS VIHPLNLHMK RFVKELGLSS VPASGGLVGV 130 140 150 160 170 180 YNGKSLVFEE SSWFIINVIK LVWRYGFQSL RMHMWVEDLL DKFMRIYRYQ SHDYAFSSVE 190 200 210 220 230 240 KLMYAIGGDD YVRLLNQTLR ENLKKAGFSE TFLNEMIAPV MKVNFGQSTD LNAFVGAVSM 250 260 270 280 290 300 TAADSNLWAV EGGNKVVCSG LLQASNSNLI SGSVMSIEKT RTKQTGTPPK MYEVVYKTGS 310 320 330 340 350 360 EIHSDFYDIV LVAAPLNRKM SNITFRNFDP PIEEFNDPYQ QLVTTLIKGE LNSTLFSSRH 370 380 390 400 410 420 KDQFGLSAIL VTDDSDMFIN SLSIVASVSH KEGPPPAVDG MHVWKTFSKD ILTKEQISKL 430 440 450 460 470 480 FLSYDYAVRK PWLSYPYYNP PQKCPSIILH DRLYYLNGIE FAASCMEMSA IAGYNAALLA 490 500 YHRWNGNEDM IDQDDLYEKL KTEL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning and characterization of the Cl(-) pump-associated 55-kDa protein in rat brain." Kitagawa K., Yagyu K., Yamamoto A., Hattori N., Omori K., Zeng X.-T., Inagaki C. Biochem. Biophys. Res. Commun. 289:363-371(2001) [PubMed: 11716481] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY. Strain: Wistar. Tissue: Brain.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Kidney.
[3]	Lubec G., Kang S.U., Lubec S. Submitted (SEP-2007) to UniProtKB Cited for: PROTEIN SEQUENCE OF 291-318 AND 420-429, MASS SPECTROMETRY. Strain: Sprague-Dawley. Tissue: Brain.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF332142 mRNA. Translation: AAK16548.1. BC078719 mRNA. Translation: AAH78719.1.
IPI	IPI00198080.
PIR	JC7782.
RefSeq	NP_659553.1.
UniGene	Rn.34505
3D structure databases
SMR	Q99ML5. Positions 33-321.
ModBase	Search...
Proteomic databases
PRIDE	Q99ML5.
Genome annotation databases
Ensembl	ENSRNOT00000022532; ENSRNOP00000022532; ENSRNOG00000016704; Rattus norvegicus. [Genome view]
GeneID	246302.
KEGG	rno:246302.
UCSC	NM_145085. rat.
Organism-specific databases
CTD	246302.
RGD	628652. Pcyox1.
Phylogenomic databases
eggNOG	roNOG04623.
HOVERGEN	Q99ML5.
InParanoid	Q99ML5.
OMA	SLRMHMW.
OrthoDB	EOG98975S.
PhylomeDB	Q99ML5.
Enzyme and pathway databases
BRENDA	1.8.3.5. 248. 3.6.3.11. 248.
Gene expression databases
Genevestigator	Q99ML5.
GermOnline	ENSRNOG00000016704. Rattus norvegicus.
Family and domain databases
InterPro	IPR010795. Prenylcys_lyase. IPR017046. Prenylcysteine_Oxase. [Graphical view]
Pfam	PF07156. Prenylcys_lyase. 1 hit. [Graphical view]
PIRSF	PIRSF036292. Prenylcysteine_oxidase. 1 hit.
ProtoNet	Search...
Other Resources
NextBio	623706.

Entry information

Entry name

PCYOX_RAT

Accession

Primary (citable) accession number: Q99ML5

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 29, 2005
Last sequence update:	June 1, 2001
Last modified:	January 19, 2010
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)