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Q99ML5 (PCYOX_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 76. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Prenylcysteine oxidase

EC=1.8.3.5
Alternative name(s):
Chloride ion pump-associated 55 kDa protein
Gene names
Name:Pcyox1
Synonyms:Clp55
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length504 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Involved in the degradation of prenylated proteins. Cleaves the thioether bond of prenyl-L-cysteines, such as farnesylcysteine and geranylgeranylcysteine By similarity.

Catalytic activity

An S-prenyl-L-cysteine + O2 + H2O = a prenal + L-cysteine + H2O2.

Cofactor

FAD By similarity.

Subcellular location

Lysosome By similarity.

Tissue specificity

Expressed mainly in cerebrum. Ref.1

Sequence similarities

Belongs to the prenylcysteine oxidase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2828 Potential
Chain29 – 504476Prenylcysteine oxidase
PRO_0000023302

Amino acid modifications

Glycosylation1961N-linked (GlcNAc...) Potential
Glycosylation3221N-linked (GlcNAc...) Potential
Glycosylation3521N-linked (GlcNAc...) Potential
Cross-link162Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) By similarity

Sequences

Sequence LengthMass (Da)Tools
Q99ML5 [UniParc].

Last modified June 1, 2001. Version 1.
Checksum: D2778026128FE9D4

FASTA50456,288
        10         20         30         40         50         60 
MGRFAATLVG SLFGLGLLLC GLGRLASAEP RAPPEKIAIV GAGIGGTSSA YYLRKKFGKD 

        70         80         90        100        110        120 
VKIDVFEREE IGGRLATLKV QGHDYEAGGS VIHPLNLHMK RFVKELGLSS VPASGGLVGV 

       130        140        150        160        170        180 
YNGKSLVFEE SSWFIINVIK LVWRYGFQSL RMHMWVEDLL DKFMRIYRYQ SHDYAFSSVE 

       190        200        210        220        230        240 
KLMYAIGGDD YVRLLNQTLR ENLKKAGFSE TFLNEMIAPV MKVNFGQSTD LNAFVGAVSM 

       250        260        270        280        290        300 
TAADSNLWAV EGGNKVVCSG LLQASNSNLI SGSVMSIEKT RTKQTGTPPK MYEVVYKTGS 

       310        320        330        340        350        360 
EIHSDFYDIV LVAAPLNRKM SNITFRNFDP PIEEFNDPYQ QLVTTLIKGE LNSTLFSSRH 

       370        380        390        400        410        420 
KDQFGLSAIL VTDDSDMFIN SLSIVASVSH KEGPPPAVDG MHVWKTFSKD ILTKEQISKL 

       430        440        450        460        470        480 
FLSYDYAVRK PWLSYPYYNP PQKCPSIILH DRLYYLNGIE FAASCMEMSA IAGYNAALLA 

       490        500 
YHRWNGNEDM IDQDDLYEKL KTEL 

« Hide

References

« Hide 'large scale' references
[1]"Molecular cloning and characterization of the Cl(-) pump-associated 55-kDa protein in rat brain."
Kitagawa K., Yagyu K., Yamamoto A., Hattori N., Omori K., Zeng X.-T., Inagaki C.
Biochem. Biophys. Res. Commun. 289:363-371(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
Strain: Wistar.
Tissue: Brain.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Kidney.
[3]Lubec G., Kang S.U., Lubec S.
Submitted (SEP-2007) to UniProtKB
Cited for: PROTEIN SEQUENCE OF 291-318 AND 420-429, IDENTIFICATION BY MASS SPECTROMETRY.
Strain: Sprague-Dawley.
Tissue: Brain.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF332142 mRNA. Translation: AAK16548.1.
BC078719 mRNA. Translation: AAH78719.1.
PIRJC7782.
RefSeqNP_659553.1. NM_145085.2.
UniGeneRn.34505.

3D structure databases

ProteinModelPortalQ99ML5.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10116.ENSRNOP00000022532.

Proteomic databases

PaxDbQ99ML5.
PRIDEQ99ML5.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000022532; ENSRNOP00000022532; ENSRNOG00000016704.
GeneID246302.
KEGGrno:246302.
UCSCRGD:628652. rat.

Organism-specific databases

CTD51449.
RGD628652. Pcyox1.

Phylogenomic databases

eggNOGNOG73316.
GeneTreeENSGT00390000011206.
HOGENOMHOG000241149.
HOVERGENHBG053532.
InParanoidQ99ML5.
KOK05906.
OMANGIECAA.
OrthoDBEOG7VB2F5.
PhylomeDBQ99ML5.
TreeFamTF329001.

Enzyme and pathway databases

BRENDA3.6.3.11. 5301.

Gene expression databases

GenevestigatorQ99ML5.

Family and domain databases

InterProIPR010795. Prenylcys_lyase.
IPR017046. Prenylcysteine_Oxase.
[Graphical view]
PfamPF07156. Prenylcys_lyase. 1 hit.
[Graphical view]
PIRSFPIRSF036292. Prenylcysteine_oxidase. 1 hit.
ProtoNetSearch...

Other

NextBio623706.
PROQ99ML5.

Entry information

Entry namePCYOX_RAT
AccessionPrimary (citable) accession number: Q99ML5
Entry history
Integrated into UniProtKB/Swiss-Prot: March 29, 2005
Last sequence update: June 1, 2001
Last modified: April 16, 2014
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families