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Protein

Bcl-2-binding component 3

Gene

Bbc3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Essential mediator of p53/TP53-dependent and p53/TP53-independent apoptosis. Functions by promoting partial unfolding of BCL2L1 and dissociation of BCL2L1 from p53/TP53 (By similarity). Regulates ER stress-induced neuronal apoptosis.By similarity2 Publications

GO - Molecular functioni

GO - Biological processi

  • activation of cysteine-type endopeptidase activity involved in apoptotic process Source: UniProtKB
  • apoptotic process Source: MGI
  • apoptotic signaling pathway Source: MGI
  • cellular response to DNA damage stimulus Source: BHF-UCL
  • cellular response to ionizing radiation Source: MGI
  • determination of adult lifespan Source: BHF-UCL
  • execution phase of apoptosis Source: UniProtKB
  • intrinsic apoptotic signaling pathway by p53 class mediator Source: MGI
  • intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator Source: MGI
  • intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress Source: UniProtKB
  • negative regulation of cell growth Source: MGI
  • negative regulation of endoplasmic reticulum calcium ion concentration Source: MGI
  • negative regulation of growth Source: UniProtKB
  • positive regulation of apoptotic process Source: MGI
  • positive regulation of cysteine-type endopeptidase activity Source: UniProtKB
  • positive regulation of intrinsic apoptotic signaling pathway Source: MGI
  • positive regulation of neuron apoptotic process Source: UniProtKB
  • positive regulation of protein homooligomerization Source: MGI
  • positive regulation of protein insertion into mitochondrial membrane involved in apoptotic signaling pathway Source: MGI
  • positive regulation of release of cytochrome c from mitochondria Source: MGI
  • positive regulation of thymocyte apoptotic process Source: BHF-UCL
  • release of cytochrome c from mitochondria Source: UniProtKB
  • release of sequestered calcium ion into cytosol Source: MGI
  • response to endoplasmic reticulum stress Source: UniProtKB
  • toxin transport Source: MGI
Complete GO annotation...

Keywords - Biological processi

Apoptosis

Names & Taxonomyi

Protein namesi
Recommended name:
Bcl-2-binding component 3
Alternative name(s):
p53 up-regulated modulator of apoptosis
Gene namesi
Name:Bbc3
Synonyms:Puma
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:2181667. Bbc3.

Subcellular locationi

  • Mitochondrion

  • Note: Localized to the mitochondria in order to induce cytochrome c release.By similarity

GO - Cellular componenti

  • endoplasmic reticulum Source: GOC
  • mitochondrial envelope Source: MGI
  • mitochondrion Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 193193Bcl-2-binding component 3PRO_0000143084Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei10 – 101PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ99ML1.
MaxQBiQ99ML1.
PaxDbiQ99ML1.
PRIDEiQ99ML1.

PTM databases

iPTMnetiQ99ML1.
PhosphoSiteiQ99ML1.

Expressioni

Inductioni

By DNA damage, glucocorticoid treatment, growth factor deprivation and p53 (By similarity). By ER stress in a DDIT3/CHOP-dependent manner.By similarity1 Publication

Gene expression databases

BgeeiENSMUSG00000002083.
CleanExiMM_BBC3.
ExpressionAtlasiQ99ML1. baseline and differential.
GenevisibleiQ99ML1. MM.

Interactioni

Subunit structurei

Interacts with BCL2 and BCL2L1/BCL-XL (By similarity). Interacts with MCL1 and BCL2A1. Interacts (via BH3 domain) with NOL3 (via CARD domain); preventing the association of BBC3 with BCL2 and resulting in activation of CASP8 (By similarity).By similarity2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
BCL2L1Q07817-13EBI-727801,EBI-287195From a different organism.

GO - Molecular functioni

Protein-protein interaction databases

BioGridi228430. 1 interaction.
DIPiDIP-29805N.
IntActiQ99ML1. 2 interactions.
STRINGi10090.ENSMUSP00000002152.

Structurei

Secondary structure

1
193
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi132 – 15120Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2ROCNMR-B130-155[»]
2VOFX-ray1.80B/D130-155[»]
ProteinModelPortaliQ99ML1.
SMRiQ99ML1. Positions 130-155.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ99ML1.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi137 – 15115BH3Add
BLAST

Domaini

The BH3 motif is intrinsically disordered.By similarity

Sequence similaritiesi

Belongs to the Bcl-2 family.Curated

Phylogenomic databases

eggNOGiENOG410IW36. Eukaryota.
ENOG41119TR. LUCA.
GeneTreeiENSGT00390000002767.
HOGENOMiHOG000060256.
HOVERGENiHBG050671.
InParanoidiQ99ML1.
KOiK10132.
OMAiQEEQHRH.
OrthoDBiEOG091G0VPS.
PhylomeDBiQ99ML1.
TreeFamiTF338776.

Family and domain databases

InterProiIPR031661. Bbc3.
[Graphical view]
PANTHERiPTHR28639. PTHR28639. 1 hit.
PfamiPF15826. PUMA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q99ML1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MARARQEGSS PEPVEGLARD SPRPFPLGRL MPSAVSCSLC EPGLPAAPAA
60 70 80 90 100
PALLPAAYLC APTAPPAVTA ALGGPRWPGG HRSRPRGPRP DGPQPSLSPA
110 120 130 140 150
QQHLESPVPS APEALAGGPT QAAPGVRVEE EEWAREIGAQ LRRMADDLNA
160 170 180 190
QYERRRQEEQ HRHRPSPWRV MYNLFMGLLP LPRDPGAPEM EPN
Length:193
Mass (Da):20,749
Last modified:June 1, 2001 - v1
Checksum:i39A98487FA86D226
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF332560 mRNA. Translation: AAK31317.1.
BC044782 mRNA. Translation: AAH44782.1.
BC060370 mRNA. Translation: AAH60370.1.
CCDSiCCDS20847.1.
RefSeqiNP_573497.1. NM_133234.2.
XP_006539640.1. XM_006539577.2.
XP_006539641.1. XM_006539578.2.
XP_006539642.1. XM_006539579.2.
XP_006539643.1. XM_006539580.1.
XP_006539644.1. XM_006539581.1.
XP_006539645.1. XM_006539582.2.
UniGeneiMm.7660.

Genome annotation databases

EnsembliENSMUST00000002152; ENSMUSP00000002152; ENSMUSG00000002083.
GeneIDi170770.
KEGGimmu:170770.
UCSCiuc009fho.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF332560 mRNA. Translation: AAK31317.1.
BC044782 mRNA. Translation: AAH44782.1.
BC060370 mRNA. Translation: AAH60370.1.
CCDSiCCDS20847.1.
RefSeqiNP_573497.1. NM_133234.2.
XP_006539640.1. XM_006539577.2.
XP_006539641.1. XM_006539578.2.
XP_006539642.1. XM_006539579.2.
XP_006539643.1. XM_006539580.1.
XP_006539644.1. XM_006539581.1.
XP_006539645.1. XM_006539582.2.
UniGeneiMm.7660.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2ROCNMR-B130-155[»]
2VOFX-ray1.80B/D130-155[»]
ProteinModelPortaliQ99ML1.
SMRiQ99ML1. Positions 130-155.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi228430. 1 interaction.
DIPiDIP-29805N.
IntActiQ99ML1. 2 interactions.
STRINGi10090.ENSMUSP00000002152.

PTM databases

iPTMnetiQ99ML1.
PhosphoSiteiQ99ML1.

Proteomic databases

EPDiQ99ML1.
MaxQBiQ99ML1.
PaxDbiQ99ML1.
PRIDEiQ99ML1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000002152; ENSMUSP00000002152; ENSMUSG00000002083.
GeneIDi170770.
KEGGimmu:170770.
UCSCiuc009fho.2. mouse.

Organism-specific databases

CTDi27113.
MGIiMGI:2181667. Bbc3.

Phylogenomic databases

eggNOGiENOG410IW36. Eukaryota.
ENOG41119TR. LUCA.
GeneTreeiENSGT00390000002767.
HOGENOMiHOG000060256.
HOVERGENiHBG050671.
InParanoidiQ99ML1.
KOiK10132.
OMAiQEEQHRH.
OrthoDBiEOG091G0VPS.
PhylomeDBiQ99ML1.
TreeFamiTF338776.

Miscellaneous databases

EvolutionaryTraceiQ99ML1.
PROiQ99ML1.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000002083.
CleanExiMM_BBC3.
ExpressionAtlasiQ99ML1. baseline and differential.
GenevisibleiQ99ML1. MM.

Family and domain databases

InterProiIPR031661. Bbc3.
[Graphical view]
PANTHERiPTHR28639. PTHR28639. 1 hit.
PfamiPF15826. PUMA. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiBBC3_MOUSE
AccessioniPrimary (citable) accession number: Q99ML1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 21, 2004
Last sequence update: June 1, 2001
Last modified: September 7, 2016
This is version 108 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.