Bcl-2-binding component 3 - Mus musculus (Mouse)

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Q99ML1 (BBC3_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified November 16, 2011. Version 70. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Bcl-2-binding component 3

Alternative name(s):
p53 up-regulated modulator of apoptosis

Gene names

Name:	Bbc3
Synonyms:	Puma

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	193 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Essential mediator of p53-dependent and p53-independent apoptosis By similarity.
Subunit structure	Interacts with BCL2 and BCL2L1/BCL-XL By similarity. Interacts with MCL1 and BCL2A1.
Subcellular location	Mitochondrion. Note: Localized to the mitochondria in order to induce cytochrome c release By similarity.
Induction	By DNA damage, glucocorticoid treatment, growth factor deprivation and p53 By similarity.
Sequence similarities	Belongs to the Bcl-2 family.

Ontologies

Keywords
Biological process	Apoptosis
Cellular component	Mitochondrion
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	DNA damage response, signal transduction by p53 class mediator resulting in induction of apoptosis Inferred from genetic interaction. Source: BHF-UCL activation of cysteine-type endopeptidase activity involved in apoptotic process Inferred from sequence or structural similarity Ref.1. Source: UniProtKB determination of adult lifespan Inferred from genetic interaction. Source: BHF-UCL negative regulation of growth Inferred from sequence or structural similarity Ref.1. Source: UniProtKB positive regulation of thymocyte apoptosis Inferred from genetic interaction. Source: BHF-UCL release of cytochrome c from mitochondria Inferred from sequence or structural similarity Ref.1. Source: UniProtKB
Cellular component	mitochondrion Inferred from sequence or structural similarity Ref.1. Source: UniProtKB
Molecular function	protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
BCL2L1	Q07817-1	3	EBI-727801,EBI-287195	From a different organism.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 193

193

Bcl-2-binding component 3

PRO_0000143084

Regions

Motif

137 – 151

BH3

Secondary structure

193

Helix Strand Turn

Details...

Sequences

Sequence

Length

Mass (Da)

Tools

Q99ML1 [UniParc].

Last modified June 1, 2001. Version 1.
Checksum: 39A98487FA86D226
Show »

FASTA

193

20,749

        10         20         30         40         50         60 
MARARQEGSS PEPVEGLARD SPRPFPLGRL MPSAVSCSLC EPGLPAAPAA PALLPAAYLC 

        70         80         90        100        110        120 
APTAPPAVTA ALGGPRWPGG HRSRPRGPRP DGPQPSLSPA QQHLESPVPS APEALAGGPT 

       130        140        150        160        170        180 
QAAPGVRVEE EEWAREIGAQ LRRMADDLNA QYERRRQEEQ HRHRPSPWRV MYNLFMGLLP 

       190 
LPRDPGAPEM EPN

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"PUMA induces the rapid apoptosis of colorectal cancer cells." Yu J., Zhang L., Hwang P.M., Kinzler K.W., Vogelstein B. Mol. Cell 7:673-682(2001) [PubMed: 11463391] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: FVB/N. Tissue: Liver and Olfactory epithelium.
[3]	"Structure of the BH3 domains from the p53-inducible BH3-only proteins Noxa and Puma in complex with Mcl-1." Day C.L., Smits C., Fan F.C., Lee E.F., Fairlie W.D., Hinds M.G. J. Mol. Biol. 380:958-971(2008) [PubMed: 18589438] [Abstract] Cited for: STRUCTURE BY NMR OF 130-155 IN COMPLEX WITH MCL1.
[4]	"Structural plasticity underpins promiscuous binding of the prosurvival protein A1." Smits C., Czabotar P.E., Hinds M.G., Day C.L. Structure 16:818-829(2008) [PubMed: 18462686] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS) OF 130-155 IN COMPLEX WITH BCL2A1.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AF332560 mRNA. Translation: AAK31317.1.
BC044782 mRNA. Translation: AAH44782.1.
BC060370 mRNA. Translation: AAH60370.1.

IPI

IPI00117809.

RefSeq

NP_573497.1. NM_133234.2.

UniGene

Mm.7660.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2ROC	NMR	-	B	130-155	[»]
2VOF	X-ray	1.80	B/D	130-155	[»]

ProteinModelPortal

Q99ML1.

SMR

Q99ML1. Positions 130-155.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-29805N.

IntAct

Q99ML1. 2 interactions.

STRING

Q99ML1.

PTM databases

PhosphoSite

Q99ML1.

Proteomic databases

PRIDE

Q99ML1.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENSMUST00000002152; ENSMUSP00000002152; ENSMUSG00000002083.

GeneID

170770.

KEGG

mmu:170770.

Organism-specific databases

CTD

27113.

MGI

MGI:2181667. Bbc3.

Phylogenomic databases

eggNOG

maNOG20446.

GeneTree

ENSGT00390000002767.

HOVERGEN

HBG050671.

InParanoid

Q99ML1.

OMA

PFPLGRL.

OrthoDB

EOG4WWRKZ.

Gene expression databases

ArrayExpress

Q99ML1.

Bgee

Q99ML1.

CleanEx

MM_BBC3.

Genevestigator

Q99ML1.

GermOnline

ENSMUSG00000002083. Mus musculus.

Family and domain databases

K10132.

PROSITE

PS01259. BH3. False negative.
[Graphical view]

ProtoNet

Search...

Other

NextBio

370391.

SOURCE

Search...

Entry information

Entry name

BBC3_MOUSE

Accession

Primary (citable) accession number: Q99ML1

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 21, 2004
Last sequence update:	June 1, 2001
Last modified:	November 16, 2011
This is version 70 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents