Reviewed, UniProtKB/Swiss-Prot Q99ML1 (BBC3_MOUSE)

Names and origin

Protein names

Recommended name:
    Bcl-2-binding component 3
Alternative name(s):
    p53 up-regulated modulator of apoptosis

Gene names

Name:	Bbc3
Synonyms:	Puma

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	193 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Essential mediator of p53-dependent and p53-independent apoptosis By similarity.
Subunit structure	Interacts with BCL2 and BCL2L1/BCL-XL By similarity. Interacts with MCL1 and BCL2A1.
Subcellular location	Mitochondrion. Note: Localized to the mitochondria in order to induce cytochrome c release By similarity.
Induction	By DNA damage, glucocorticoid treatment, growth factor deprivation and p53 By similarity.
Sequence similarities	Belongs to the Bcl-2 family.

Ontologies

Keywords
Biological process	Apoptosis
Cellular component	Mitochondrion
Technical term	3D-structure
Gene Ontology (GO)
Biological process	DNA damage response, signal transduction by p53 class mediator resulting in induction of apoptosis Inferred from mutant phenotype. Source: MGI activation of caspase activity Ref.1 Inferred from sequence or structural similarity. Source: UniProtKB negative regulation of growth Ref.1 Inferred from sequence or structural similarity. Source: UniProtKB release of cytochrome c from mitochondria Ref.1 Inferred from sequence or structural similarity. Source: UniProtKB
Cellular component	mitochondrion Ref.1 Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	protein binding Ref.1 Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
BCL2L1	Q07817-1	2	EBI-727801,EBI-287195	From a different organism.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 193

193

Bcl-2-binding component 3

PRO_0000143084

Regions

Motif

137 – 151

15

BH3

Secondary structure

1

.

.

.

193

Helix Strand Turn

Details...

Helix

132 – 151

20

Sequences

	Sequence		Length	Mass (Da)
	Q99ML1-1 [UniParc]. Last modified June 1, 2001. Version 1. Checksum: 39A98487FA86D226	FASTA	193	20,749
	10 20 30 40 50 60 MARARQEGSS PEPVEGLARD SPRPFPLGRL MPSAVSCSLC EPGLPAAPAA PALLPAAYLC 70 80 90 100 110 120 APTAPPAVTA ALGGPRWPGG HRSRPRGPRP DGPQPSLSPA QQHLESPVPS APEALAGGPT 130 140 150 160 170 180 QAAPGVRVEE EEWAREIGAQ LRRMADDLNA QYERRRQEEQ HRHRPSPWRV MYNLFMGLLP 190 LPRDPGAPEM EPN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"PUMA induces the rapid apoptosis of colorectal cancer cells." Yu J., Zhang L., Hwang P.M., Kinzler K.W., Vogelstein B. Mol. Cell 7:673-682(2001) [PubMed: 11463391] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: FVB/N. Tissue: Liver and Olfactory epithelium.
[3]	"Structure of the BH3 domains from the p53-inducible BH3-only proteins Noxa and Puma in complex with Mcl-1." Day C.L., Smits C., Fan F.C., Lee E.F., Fairlie W.D., Hinds M.G. J. Mol. Biol. 380:958-971(2008) [PubMed: 18589438] [Abstract] Cited for: STRUCTURE BY NMR OF 130-155 IN COMPLEX WITH MCL1.
[4]	"Structural plasticity underpins promiscuous binding of the prosurvival protein A1." Smits C., Czabotar P.E., Hinds M.G., Day C.L. Structure 16:818-829(2008) [PubMed: 18462686] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS) OF 130-155 IN COMPLEX WITH BCL2A1.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

AF332560 mRNA. Translation: AAK31317.1.
BC044782 mRNA. Translation: AAH44782.1.
BC060370 mRNA. Translation: AAH60370.1.

IPI

IPI00117809.

RefSeq

NP_573497.1.

UniGene

Mm.7660

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2ROC	NMR	-	B	130-155	[»]
2VOF	X-ray	1.80	B/D	130-155	[»]

ModBase

Search...

Protein-protein interaction databases

IntAct

Q99ML1. 3 interactions.

Genome annotation databases

Ensembl

ENSMUSG00000002083. Mus musculus. [Contig view]

GeneID

170770.

KEGG

mmu:170770.

Organism-specific databases

MGI

MGI:2181667. Bbc3.

Phylogenomic databases

HOGENOM

Q99ML1.

HOVERGEN

Q99ML1.

OMA

Q99ML1. RDGPRPF.

Gene expression databases

ArrayExpress

Q99ML1.

Bgee

Q99ML1.

CleanEx

MM_BBC3.

GermOnline

ENSMUSG00000002083. Mus musculus.

Family and domain databases

InterPro

IPR000712. Bcl2_BH.
[Graphical view]

PROSITE

PS01259. BH3. False negative.
[Graphical view]

ProtoNet

Search...

Other Resources

NextBio

370391.

SOURCE

Search...

Entry information

Entry name

BBC3_MOUSE

Accession

Primary (citable) accession number: Q99ML1

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 21, 2004
Last sequence update:	June 1, 2001
Last modified:	June 16, 2009
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)