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Protein

Bcl-2-binding component 3

Gene

Bbc3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Essential mediator of p53/TP53-dependent and p53/TP53-independent apoptosis. Functions by promoting partial unfolding of BCL2L1 and dissociation of BCL2L1 from p53/TP53 (By similarity). Regulates ER stress-induced neuronal apoptosis.By similarity2 Publications

GO - Molecular functioni

GO - Biological processi

  • activation of cysteine-type endopeptidase activity involved in apoptotic process Source: UniProtKB
  • apoptotic process Source: MGI
  • apoptotic signaling pathway Source: MGI
  • cellular response to DNA damage stimulus Source: BHF-UCL
  • cellular response to ionizing radiation Source: MGI
  • determination of adult lifespan Source: BHF-UCL
  • execution phase of apoptosis Source: UniProtKB
  • intrinsic apoptotic signaling pathway by p53 class mediator Source: MGI
  • intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator Source: MGI
  • intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress Source: UniProtKB
  • negative regulation of cell growth Source: MGI
  • negative regulation of endoplasmic reticulum calcium ion concentration Source: MGI
  • negative regulation of growth Source: UniProtKB
  • positive regulation of apoptotic process Source: MGI
  • positive regulation of cysteine-type endopeptidase activity Source: UniProtKB
  • positive regulation of intrinsic apoptotic signaling pathway Source: MGI
  • positive regulation of neuron apoptotic process Source: UniProtKB
  • positive regulation of protein homooligomerization Source: MGI
  • positive regulation of protein insertion into mitochondrial membrane involved in apoptotic signaling pathway Source: MGI
  • positive regulation of release of cytochrome c from mitochondria Source: MGI
  • positive regulation of thymocyte apoptotic process Source: BHF-UCL
  • release of cytochrome c from mitochondria Source: UniProtKB
  • release of sequestered calcium ion into cytosol Source: MGI
  • response to endoplasmic reticulum stress Source: UniProtKB
  • toxin transport Source: MGI

Keywordsi

Biological processApoptosis

Names & Taxonomyi

Protein namesi
Recommended name:
Bcl-2-binding component 3
Alternative name(s):
p53 up-regulated modulator of apoptosis
Gene namesi
Name:Bbc3
Synonyms:Puma
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:2181667 Bbc3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001430841 – 193Bcl-2-binding component 3Add BLAST193

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei10PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ99ML1
MaxQBiQ99ML1
PaxDbiQ99ML1
PRIDEiQ99ML1

PTM databases

iPTMnetiQ99ML1
PhosphoSitePlusiQ99ML1

Expressioni

Inductioni

By DNA damage, glucocorticoid treatment, growth factor deprivation and p53 (By similarity). By ER stress in a DDIT3/CHOP-dependent manner.By similarity1 Publication

Gene expression databases

BgeeiENSMUSG00000002083
CleanExiMM_BBC3
ExpressionAtlasiQ99ML1 baseline and differential
GenevisibleiQ99ML1 MM

Interactioni

Subunit structurei

Interacts with BCL2 and BCL2L1/BCL-XL (By similarity). Interacts with MCL1 and BCL2A1. Interacts (via BH3 domain) with NOL3 (via CARD domain); preventing the association of BBC3 with BCL2 and resulting in activation of CASP8 (By similarity).By similarity2 Publications

Binary interactionsi

Show more details

GO - Molecular functioni

Protein-protein interaction databases

BioGridi228430, 1 interactor
CORUMiQ99ML1
DIPiDIP-29805N
ELMiQ99ML1
IntActiQ99ML1, 3 interactors
STRINGi10090.ENSMUSP00000002152

Structurei

Secondary structure

1193
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi132 – 151Combined sources20

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2ROCNMR-B130-155[»]
2VOFX-ray1.80B/D130-155[»]
ProteinModelPortaliQ99ML1
SMRiQ99ML1
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ99ML1

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi137 – 151BH3Add BLAST15

Domaini

The BH3 motif is intrinsically disordered.By similarity

Sequence similaritiesi

Belongs to the Bcl-2 family.Curated

Phylogenomic databases

eggNOGiENOG410IW36 Eukaryota
ENOG41119TR LUCA
GeneTreeiENSGT00390000002767
HOGENOMiHOG000060256
HOVERGENiHBG050671
InParanoidiQ99ML1
KOiK10132
OMAiGPRPDXR
OrthoDBiEOG091G0VPS
PhylomeDBiQ99ML1
TreeFamiTF338776

Family and domain databases

InterProiView protein in InterPro
IPR031661 Bbc3
PANTHERiPTHR28639 PTHR28639, 1 hit
PfamiView protein in Pfam
PF15826 PUMA, 1 hit

Sequencei

Sequence statusi: Complete.

Q99ML1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MARARQEGSS PEPVEGLARD SPRPFPLGRL MPSAVSCSLC EPGLPAAPAA
60 70 80 90 100
PALLPAAYLC APTAPPAVTA ALGGPRWPGG HRSRPRGPRP DGPQPSLSPA
110 120 130 140 150
QQHLESPVPS APEALAGGPT QAAPGVRVEE EEWAREIGAQ LRRMADDLNA
160 170 180 190
QYERRRQEEQ HRHRPSPWRV MYNLFMGLLP LPRDPGAPEM EPN
Length:193
Mass (Da):20,749
Last modified:June 1, 2001 - v1
Checksum:i39A98487FA86D226
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF332560 mRNA Translation: AAK31317.1
BC044782 mRNA Translation: AAH44782.1
BC060370 mRNA Translation: AAH60370.1
CCDSiCCDS20847.1
RefSeqiNP_573497.1, NM_133234.2
XP_006539640.1, XM_006539577.3
XP_006539641.1, XM_006539578.3
XP_006539642.1, XM_006539579.3
XP_006539643.1, XM_006539580.2
XP_006539644.1, XM_006539581.1
XP_006539645.1, XM_006539582.2
UniGeneiMm.7660

Genome annotation databases

EnsembliENSMUST00000002152; ENSMUSP00000002152; ENSMUSG00000002083
GeneIDi170770
KEGGimmu:170770
UCSCiuc009fho.2 mouse

Similar proteinsi

Entry informationi

Entry nameiBBC3_MOUSE
AccessioniPrimary (citable) accession number: Q99ML1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 21, 2004
Last sequence update: June 1, 2001
Last modified: March 28, 2018
This is version 118 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

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