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Q99MK9

- RASF1_MOUSE

UniProt

Q99MK9 - RASF1_MOUSE

Protein

Ras association domain-containing protein 1

Gene

Rassf1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 108 (01 Oct 2014)
      Sequence version 1 (01 Jun 2001)
      Previous versions | rss
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    Functioni

    Potential tumor suppressor. Required for death receptor-dependent apoptosis. Mediates activation of Mediates activation of STK3/MST2 and STK4/MST1 during Fas-induced apoptosis by preventing their dephosphorylation. When associated with MOAP1, promotes BAX conformational change and translocation to mitochondrial membranes in response to TNF and TNFSF10 stimulation. Isoform A interacts with CDC20, an activator of the anaphase-promoting complex, APC, resulting in the inhibition of APC activity and mitotic progression. Inhibits proliferation by negatively regulating cell cycle progression at the level of G1/S-phase transition by regulating accumulation of cyclin D1 protein. Isoform C has been shown not to perform these roles, no function has been identified for this isoform. Isoform A disrupts interactions among MDM2, DAXX and USP7, thus contributing to the efficient activation of TP53 by promoting MDM2 self-ubiquitination in cell-cycle checkpoint control in response to DNA damage By similarity.By similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri51 – 10151Phorbol-ester/DAG-typePROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW
    2. protein binding Source: MGI

    GO - Biological processi

    1. cell cycle arrest Source: UniProtKB
    2. cellular response to DNA damage stimulus Source: UniProtKB
    3. negative regulation of cell cycle arrest Source: UniProtKB
    4. positive regulation of protein ubiquitination Source: UniProtKB
    5. protein stabilization Source: UniProtKB
    6. Ras protein signal transduction Source: UniProtKB

    Keywords - Biological processi

    Cell cycle

    Keywords - Ligandi

    Metal-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ras association domain-containing protein 1
    Alternative name(s):
    Protein 123F2
    Gene namesi
    Name:Rassf1Imported
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 9

    Organism-specific databases

    MGIiMGI:1928386. Rassf1.

    Subcellular locationi

    Isoform A : Cytoplasmcytoskeleton By similarity. Cytoplasmcytoskeletonmicrotubule organizing centercentrosome By similarity. Cytoplasmcytoskeletonspindle By similarity. Cytoplasmcytoskeletonspindle pole By similarity
    Note: Localizes to cytoplasmic microtubules during interphase, to bipolar centrosomes associated with microtubules during prophase, to spindle fibers and spindle poles at metaphase and anaphase, to the midzone during early telophase, and to the midbody in late telophase in cells. Colocalizes with MDM2 in the nucleus By similarity.By similarity
    Isoform C : Nucleus By similarity
    Note: Predominantly nuclear.By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-KW
    2. microtubule Source: UniProtKB-KW
    3. microtubule cytoskeleton Source: UniProtKB
    4. microtubule organizing center Source: UniProtKB-SubCell
    5. nucleus Source: UniProtKB
    6. plasma membrane Source: Ensembl
    7. spindle pole Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm, Cytoskeleton, Microtubule, Nucleus

    Pathology & Biotechi

    Keywords - Diseasei

    Tumor suppressor

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 340339Ras association domain-containing protein 1PRO_0000068892Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylserineBy similarity
    Modified residuei2 – 21PhosphoserineBy similarity

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    PRIDEiQ99MK9.

    PTM databases

    PhosphoSiteiQ99MK9.

    Expressioni

    Gene expression databases

    ArrayExpressiQ99MK9.
    BgeeiQ99MK9.
    GenevestigatoriQ99MK9.

    Interactioni

    Subunit structurei

    Interacts with MAP1S and XPA. Binds to the N-terminal of CDC20 during prometaphase. Binds to STK3/MST2 and STK4/MST1. Isoform A interacts with MOAP1 and E4F1. Recruited to the TNFRSF1A and TNFRSF10A complexes in response to their respective cognate ligand, after internalization. Can self-associate. Isoform A interacts with RSSF5 and probably associates with HRAS via a RSSF1 isoform A-RSSF5 heterodimer. Part of a complex with MDM2, DAXX, RASSF1 and USP7. Isoform A interacts (via C-terminus) with DAXX (via N-terminus); the interaction is independent of MDM2 and TP53. Isoform A interacts (via N-terminus) with MDM2 (via C-terminus); the interaction is independent of TP53 By similarity.By similarity

    Protein-protein interaction databases

    BioGridi207877. 11 interactions.
    IntActiQ99MK9. 10 interactions.
    MINTiMINT-5197358.

    Structurei

    3D structure databases

    ProteinModelPortaliQ99MK9.
    SMRiQ99MK9. Positions 49-101, 134-286, 290-334.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini194 – 28895Ras-associatingPROSITE-ProRule annotationAdd
    BLAST
    Domaini290 – 33748SARAHPROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni2 – 115114Mediates interaction with E4F1By similarityAdd
    BLAST
    Regioni311 – 3144MOAP1-bindingBy similarity

    Sequence similaritiesi

    Contains 1 phorbol-ester/DAG-type zinc finger.PROSITE-ProRule annotation
    Contains 1 Ras-associating domain.PROSITE-ProRule annotation
    Contains 1 SARAH domain.PROSITE-ProRule annotation

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri51 – 10151Phorbol-ester/DAG-typePROSITE-ProRule annotationAdd
    BLAST

    Keywords - Domaini

    Zinc-finger

    Phylogenomic databases

    eggNOGiNOG308852.
    GeneTreeiENSGT00390000003367.
    HOGENOMiHOG000013025.
    HOVERGENiHBG054362.
    KOiK09850.
    OMAiTTSSGYC.
    OrthoDBiEOG7TF796.
    PhylomeDBiQ99MK9.
    TreeFamiTF319243.

    Family and domain databases

    InterProiIPR002219. Prot_Kinase_C-like_PE/DAG-bd.
    IPR000159. Ras-assoc.
    IPR011524. SARAH_dom.
    IPR029071. Ubiquitin-rel_dom.
    [Graphical view]
    PfamiPF00130. C1_1. 1 hit.
    PF00788. RA. 1 hit.
    [Graphical view]
    SMARTiSM00109. C1. 1 hit.
    SM00314. RA. 1 hit.
    [Graphical view]
    SUPFAMiSSF54236. SSF54236. 1 hit.
    PROSITEiPS50200. RA. 1 hit.
    PS50951. SARAH. 1 hit.
    PS00479. ZF_DAG_PE_1. 1 hit.
    PS50081. ZF_DAG_PE_2. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 2 isoformsi produced by alternative promoter usage. Align

    Isoform A (identifier: Q99MK9-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MSAEPELIEL RELAPSGRIG PGRTRLERAN ALRIAPGTTR NPSQQHVPGR    50
    GHRFQPAGPT THTWCDLCGD FIWGVVRKGL QCAHCKFTCH YRCRALVCLD 100
    CCGPRDLGWD SALERDTNVD EAVERETPDL SQAETEQKIK DYNGQINSNL 150
    FMSLNKDGSY TGFIKVQLKL VRPVSVPSSK KPPSLQDARR GTGRSTAVKR 200
    RTSFYLPKDA IKHLHVLSRT RAREVIEALL RKFMVVDDPR KFALFERTER 250
    HGQVYLRKLS DDEQPLKLRL LAGPSEKALS FVLKENDSGE VNWDAFSMPE 300
    LHNFLRILQR EEEEHLRQIL QKYSRCRQKI QEALHACPLG 340
    Length:340
    Mass (Da):38,789
    Last modified:June 1, 2001 - v1
    Checksum:i7DEBA4AEE705757F
    GO
    Isoform C (identifier: Q99MK9-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         2-70: Missing.
         71-119: FIWGVVRKGL...DSALERDTNV → MGEAETPSFE...SLVRRPRRDQ

    Show »
    Length:271
    Mass (Da):31,324
    Checksum:iA7E1074A043ED651
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei2 – 7069Missing in isoform C. 2 PublicationsVSP_050779Add
    BLAST
    Alternative sequencei71 – 11949FIWGV…RDTNV → MGEAETPSFEMTWSSTTSSG YCSQEDSDSELEQYFTARTS LVRRPRRDQ in isoform C. 3 PublicationsVSP_050780Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF132851 mRNA. Translation: AAD30061.1.
    AF333027 Genomic DNA. Translation: AAK21200.1.
    AF333027 Genomic DNA. Translation: AAK21201.1.
    BC002173 mRNA. Translation: AAH02173.1.
    CCDSiCCDS57699.1. [Q99MK9-1]
    RefSeqiNP_001230677.1. NM_001243748.1. [Q99MK9-1]
    NP_062687.1. NM_019713.4.
    UniGeneiMm.12091.
    Mm.488708.

    Genome annotation databases

    EnsembliENSMUST00000010211; ENSMUSP00000010211; ENSMUSG00000010067.
    ENSMUST00000093786; ENSMUSP00000091301; ENSMUSG00000010067. [Q99MK9-1]
    GeneIDi56289.
    KEGGimmu:56289.
    UCSCiuc012hag.2. mouse. [Q99MK9-1]

    Keywords - Coding sequence diversityi

    Alternative promoter usage

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF132851 mRNA. Translation: AAD30061.1 .
    AF333027 Genomic DNA. Translation: AAK21200.1 .
    AF333027 Genomic DNA. Translation: AAK21201.1 .
    BC002173 mRNA. Translation: AAH02173.1 .
    CCDSi CCDS57699.1. [Q99MK9-1 ]
    RefSeqi NP_001230677.1. NM_001243748.1. [Q99MK9-1 ]
    NP_062687.1. NM_019713.4.
    UniGenei Mm.12091.
    Mm.488708.

    3D structure databases

    ProteinModelPortali Q99MK9.
    SMRi Q99MK9. Positions 49-101, 134-286, 290-334.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 207877. 11 interactions.
    IntActi Q99MK9. 10 interactions.
    MINTi MINT-5197358.

    PTM databases

    PhosphoSitei Q99MK9.

    Proteomic databases

    PRIDEi Q99MK9.

    Protocols and materials databases

    DNASUi 56289.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000010211 ; ENSMUSP00000010211 ; ENSMUSG00000010067 .
    ENSMUST00000093786 ; ENSMUSP00000091301 ; ENSMUSG00000010067 . [Q99MK9-1 ]
    GeneIDi 56289.
    KEGGi mmu:56289.
    UCSCi uc012hag.2. mouse. [Q99MK9-1 ]

    Organism-specific databases

    CTDi 11186.
    MGIi MGI:1928386. Rassf1.

    Phylogenomic databases

    eggNOGi NOG308852.
    GeneTreei ENSGT00390000003367.
    HOGENOMi HOG000013025.
    HOVERGENi HBG054362.
    KOi K09850.
    OMAi TTSSGYC.
    OrthoDBi EOG7TF796.
    PhylomeDBi Q99MK9.
    TreeFami TF319243.

    Miscellaneous databases

    ChiTaRSi RASSF1. mouse.
    NextBioi 312192.
    PROi Q99MK9.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q99MK9.
    Bgeei Q99MK9.
    Genevestigatori Q99MK9.

    Family and domain databases

    InterProi IPR002219. Prot_Kinase_C-like_PE/DAG-bd.
    IPR000159. Ras-assoc.
    IPR011524. SARAH_dom.
    IPR029071. Ubiquitin-rel_dom.
    [Graphical view ]
    Pfami PF00130. C1_1. 1 hit.
    PF00788. RA. 1 hit.
    [Graphical view ]
    SMARTi SM00109. C1. 1 hit.
    SM00314. RA. 1 hit.
    [Graphical view ]
    SUPFAMi SSF54236. SSF54236. 1 hit.
    PROSITEi PS50200. RA. 1 hit.
    PS50951. SARAH. 1 hit.
    PS00479. ZF_DAG_PE_1. 1 hit.
    PS50081. ZF_DAG_PE_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM C).
    2. "A mouse locus containing the ortholog of the human RASSF1 tumor suppressor gene."
      Dammann R., Pfeifer G.P.
      Submitted (JAN-2001) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], ALTERNATIVE PROMOTER USAGE.
      Strain: 129/SvImported.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM C).
      Tissue: Mammary glandImported.

    Entry informationi

    Entry nameiRASF1_MOUSE
    AccessioniPrimary (citable) accession number: Q99MK9
    Secondary accession number(s): Q9WUF5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 19, 2004
    Last sequence update: June 1, 2001
    Last modified: October 1, 2014
    This is version 108 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3