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Protein

Ras association domain-containing protein 1

Gene

Rassf1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Potential tumor suppressor. Required for death receptor-dependent apoptosis. Mediates activation of Mediates activation of STK3/MST2 and STK4/MST1 during Fas-induced apoptosis by preventing their dephosphorylation. When associated with MOAP1, promotes BAX conformational change and translocation to mitochondrial membranes in response to TNF and TNFSF10 stimulation. Isoform A interacts with CDC20, an activator of the anaphase-promoting complex, APC, resulting in the inhibition of APC activity and mitotic progression. Inhibits proliferation by negatively regulating cell cycle progression at the level of G1/S-phase transition by regulating accumulation of cyclin D1 protein. Isoform C has been shown not to perform these roles, no function has been identified for this isoform. Isoform A disrupts interactions among MDM2, DAXX and USP7, thus contributing to the efficient activation of TP53 by promoting MDM2 self-ubiquitination in cell-cycle checkpoint control in response to DNA damage (By similarity).By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri51 – 10151Phorbol-ester/DAG-typePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. identical protein binding Source: MGI
  2. metal ion binding Source: UniProtKB-KW
  3. protein N-terminus binding Source: MGI

GO - Biological processi

  1. cell cycle arrest Source: UniProtKB
  2. cellular response to DNA damage stimulus Source: UniProtKB
  3. negative regulation of cell cycle arrest Source: UniProtKB
  4. positive regulation of protein ubiquitination Source: UniProtKB
  5. protein stabilization Source: UniProtKB
  6. Ras protein signal transduction Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

Cell cycle

Keywords - Ligandi

Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Ras association domain-containing protein 1
Alternative name(s):
Protein 123F2
Gene namesi
Name:Rassf1Imported
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 9

Organism-specific databases

MGIiMGI:1928386. Rassf1.

Subcellular locationi

Isoform A : Cytoplasmcytoskeleton By similarity. Cytoplasmcytoskeletonmicrotubule organizing centercentrosome By similarity. Cytoplasmcytoskeletonspindle By similarity. Cytoplasmcytoskeletonspindle pole By similarity
Note: Localizes to cytoplasmic microtubules during interphase, to bipolar centrosomes associated with microtubules during prophase, to spindle fibers and spindle poles at metaphase and anaphase, to the midzone during early telophase, and to the midbody in late telophase in cells. Colocalizes with MDM2 in the nucleus (By similarity).By similarity
Isoform C : Nucleus By similarity
Note: Predominantly nuclear.By similarity

GO - Cellular componenti

  1. cytoplasm Source: MGI
  2. microtubule Source: UniProtKB-KW
  3. microtubule cytoskeleton Source: UniProtKB
  4. microtubule organizing center Source: UniProtKB-SubCell
  5. nucleus Source: UniProtKB
  6. plasma membrane Source: MGI
  7. spindle pole Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Microtubule, Nucleus

Pathology & Biotechi

Keywords - Diseasei

Tumor suppressor

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 340339Ras association domain-containing protein 1PRO_0000068892Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserineBy similarity
Modified residuei2 – 21PhosphoserineBy similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiQ99MK9.
PRIDEiQ99MK9.

PTM databases

PhosphoSiteiQ99MK9.

Expressioni

Gene expression databases

BgeeiQ99MK9.
ExpressionAtlasiQ99MK9. baseline and differential.
GenevestigatoriQ99MK9.

Interactioni

Subunit structurei

Interacts with MAP1S and XPA. Binds to the N-terminal of CDC20 during prometaphase. Binds to STK3/MST2 and STK4/MST1. Isoform A interacts with MOAP1 and E4F1. Recruited to the TNFRSF1A and TNFRSF10A complexes in response to their respective cognate ligand, after internalization. Can self-associate. Isoform A interacts with RSSF5 and probably associates with HRAS via a RSSF1 isoform A-RSSF5 heterodimer. Part of a complex with MDM2, DAXX, RASSF1 and USP7. Isoform A interacts (via C-terminus) with DAXX (via N-terminus); the interaction is independent of MDM2 and TP53. Isoform A interacts (via N-terminus) with MDM2 (via C-terminus); the interaction is independent of TP53 (By similarity).By similarity

Protein-protein interaction databases

BioGridi207877. 11 interactions.
IntActiQ99MK9. 10 interactions.
MINTiMINT-5197358.

Structurei

3D structure databases

ProteinModelPortaliQ99MK9.
SMRiQ99MK9. Positions 49-101, 134-286, 290-334.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini194 – 28895Ras-associatingPROSITE-ProRule annotationAdd
BLAST
Domaini290 – 33748SARAHPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni2 – 115114Mediates interaction with E4F1By similarityAdd
BLAST
Regioni311 – 3144MOAP1-bindingBy similarity

Sequence similaritiesi

Contains 1 phorbol-ester/DAG-type zinc finger.PROSITE-ProRule annotation
Contains 1 Ras-associating domain.PROSITE-ProRule annotation
Contains 1 SARAH domain.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri51 – 10151Phorbol-ester/DAG-typePROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiNOG308852.
GeneTreeiENSGT00390000003367.
HOGENOMiHOG000013025.
HOVERGENiHBG054362.
InParanoidiQ99MK9.
KOiK09850.
OrthoDBiEOG7TF796.
PhylomeDBiQ99MK9.
TreeFamiTF319243.

Family and domain databases

InterProiIPR002219. PE/DAG-bd.
IPR000159. Ras-assoc.
IPR011524. SARAH_dom.
IPR029071. Ubiquitin-rel_dom.
[Graphical view]
PfamiPF00130. C1_1. 1 hit.
PF00788. RA. 1 hit.
[Graphical view]
SMARTiSM00109. C1. 1 hit.
SM00314. RA. 1 hit.
[Graphical view]
SUPFAMiSSF54236. SSF54236. 1 hit.
PROSITEiPS50200. RA. 1 hit.
PS50951. SARAH. 1 hit.
PS00479. ZF_DAG_PE_1. 1 hit.
PS50081. ZF_DAG_PE_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative promoter usage. AlignAdd to basket

Isoform A (identifier: Q99MK9-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSAEPELIEL RELAPSGRIG PGRTRLERAN ALRIAPGTTR NPSQQHVPGR
60 70 80 90 100
GHRFQPAGPT THTWCDLCGD FIWGVVRKGL QCAHCKFTCH YRCRALVCLD
110 120 130 140 150
CCGPRDLGWD SALERDTNVD EAVERETPDL SQAETEQKIK DYNGQINSNL
160 170 180 190 200
FMSLNKDGSY TGFIKVQLKL VRPVSVPSSK KPPSLQDARR GTGRSTAVKR
210 220 230 240 250
RTSFYLPKDA IKHLHVLSRT RAREVIEALL RKFMVVDDPR KFALFERTER
260 270 280 290 300
HGQVYLRKLS DDEQPLKLRL LAGPSEKALS FVLKENDSGE VNWDAFSMPE
310 320 330 340
LHNFLRILQR EEEEHLRQIL QKYSRCRQKI QEALHACPLG
Length:340
Mass (Da):38,789
Last modified:June 1, 2001 - v1
Checksum:i7DEBA4AEE705757F
GO
Isoform C (identifier: Q99MK9-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-70: Missing.
     71-119: FIWGVVRKGL...DSALERDTNV → MGEAETPSFE...SLVRRPRRDQ

Show »
Length:270
Mass (Da):31,193
Checksum:i6E11302FA93EC0BC
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 7070Missing in isoform C. 2 PublicationsVSP_050779Add
BLAST
Alternative sequencei71 – 11949FIWGV…RDTNV → MGEAETPSFEMTWSSTTSSG YCSQEDSDSELEQYFTARTS LVRRPRRDQ in isoform C. 3 PublicationsVSP_050780Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF132851 mRNA. Translation: AAD30061.1.
AF333027 Genomic DNA. Translation: AAK21200.1.
AF333027 Genomic DNA. Translation: AAK21201.1.
BC002173 mRNA. Translation: AAH02173.1.
CCDSiCCDS23494.1. [Q99MK9-2]
CCDS57699.1. [Q99MK9-1]
RefSeqiNP_001230677.1. NM_001243748.1. [Q99MK9-1]
NP_062687.1. NM_019713.4. [Q99MK9-2]
UniGeneiMm.12091.
Mm.488708.

Genome annotation databases

EnsembliENSMUST00000010211; ENSMUSP00000010211; ENSMUSG00000010067. [Q99MK9-2]
ENSMUST00000093786; ENSMUSP00000091301; ENSMUSG00000010067. [Q99MK9-1]
GeneIDi56289.
KEGGimmu:56289.
UCSCiuc012hag.2. mouse. [Q99MK9-1]

Keywords - Coding sequence diversityi

Alternative promoter usage

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF132851 mRNA. Translation: AAD30061.1.
AF333027 Genomic DNA. Translation: AAK21200.1.
AF333027 Genomic DNA. Translation: AAK21201.1.
BC002173 mRNA. Translation: AAH02173.1.
CCDSiCCDS23494.1. [Q99MK9-2]
CCDS57699.1. [Q99MK9-1]
RefSeqiNP_001230677.1. NM_001243748.1. [Q99MK9-1]
NP_062687.1. NM_019713.4. [Q99MK9-2]
UniGeneiMm.12091.
Mm.488708.

3D structure databases

ProteinModelPortaliQ99MK9.
SMRiQ99MK9. Positions 49-101, 134-286, 290-334.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi207877. 11 interactions.
IntActiQ99MK9. 10 interactions.
MINTiMINT-5197358.

PTM databases

PhosphoSiteiQ99MK9.

Proteomic databases

MaxQBiQ99MK9.
PRIDEiQ99MK9.

Protocols and materials databases

DNASUi56289.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000010211; ENSMUSP00000010211; ENSMUSG00000010067. [Q99MK9-2]
ENSMUST00000093786; ENSMUSP00000091301; ENSMUSG00000010067. [Q99MK9-1]
GeneIDi56289.
KEGGimmu:56289.
UCSCiuc012hag.2. mouse. [Q99MK9-1]

Organism-specific databases

CTDi11186.
MGIiMGI:1928386. Rassf1.

Phylogenomic databases

eggNOGiNOG308852.
GeneTreeiENSGT00390000003367.
HOGENOMiHOG000013025.
HOVERGENiHBG054362.
InParanoidiQ99MK9.
KOiK09850.
OrthoDBiEOG7TF796.
PhylomeDBiQ99MK9.
TreeFamiTF319243.

Miscellaneous databases

ChiTaRSiRassf1. mouse.
NextBioi312192.
PROiQ99MK9.
SOURCEiSearch...

Gene expression databases

BgeeiQ99MK9.
ExpressionAtlasiQ99MK9. baseline and differential.
GenevestigatoriQ99MK9.

Family and domain databases

InterProiIPR002219. PE/DAG-bd.
IPR000159. Ras-assoc.
IPR011524. SARAH_dom.
IPR029071. Ubiquitin-rel_dom.
[Graphical view]
PfamiPF00130. C1_1. 1 hit.
PF00788. RA. 1 hit.
[Graphical view]
SMARTiSM00109. C1. 1 hit.
SM00314. RA. 1 hit.
[Graphical view]
SUPFAMiSSF54236. SSF54236. 1 hit.
PROSITEiPS50200. RA. 1 hit.
PS50951. SARAH. 1 hit.
PS00479. ZF_DAG_PE_1. 1 hit.
PS50081. ZF_DAG_PE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM C).
  2. "A mouse locus containing the ortholog of the human RASSF1 tumor suppressor gene."
    Dammann R., Pfeifer G.P.
    Submitted (JAN-2001) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], ALTERNATIVE PROMOTER USAGE.
    Strain: 129/SvImported.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM C).
    Tissue: Mammary glandImported.

Entry informationi

Entry nameiRASF1_MOUSE
AccessioniPrimary (citable) accession number: Q99MK9
Secondary accession number(s): Q9WUF5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 19, 2004
Last sequence update: June 1, 2001
Last modified: March 4, 2015
This is version 113 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.