Q99MK9 (RASF1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ras association domain-containing protein 1

Alternative name(s):
Protein 123F2

Gene names

Name:

Rassf1

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	340 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Potential tumor suppressor. Required for death receptor-dependent apoptosis. Mediates activation of Mediates activation of STK3/MST2 and STK4/MST1 during Fas-induced apoptosis by preventing their dephosphorylation. When associated with MOAP1, promotes BAX conformational change and translocation to mitochondrial membranes in response to TNF and TNFSF10 stimulation. Isoform A interacts with CDC20, an activator of the anaphase-promoting complex, APC, resulting in the inhibition of APC activity and mitotic progression. Inhibits proliferation by negatively regulating cell cycle progression at the level of G1/S-phase transition by regulating accumulation of cyclin D1 protein. Isoform C has been shown not to perform these roles, no function has been identified for this isoform. Isoform A disrupts interactions among MDM2, DAXX and USP7, thus contributing to the efficient activation of TP53 by promoting MDM2 self-ubiquitination in cell-cycle checkpoint control in response to DNA damage By similarity.
Subunit structure	Interacts with MAP1S and XPA. Binds to the N-terminal of CDC20 during prometaphase. Binds to STK3/MST2 and STK4/MST1. Isoform A interacts with MOAP1 and E4F1. Recruited to the TNFRSF1A and TNFRSF10A complexes in response to their respective cognate ligand, after internalization. Can self-associate. Isoform A interacts with RSSF5 and probably associates with HRAS1 via a RSSF1 isoform A-RSSF5 heterodimer. Part of a complex with MDM2, DAXX, RASSF1 and USP7. Isoform A interacts (via C-terminus) with DAXX (via N-terminus); the interaction is independent of MDM2 and TP53. Isoform A interacts (via N-terminus) with MDM2 (via C-terminus); the interaction is independent of TP53 By similarity.
Subcellular location	Isoform A: Cytoplasm › cytoskeleton By similarity. Cytoplasm › cytoskeleton › centrosome By similarity. Cytoplasm › cytoskeleton › spindle By similarity. Cytoplasm › cytoskeleton › spindle pole By similarity. Note: Localizes to cytoplasmic microtubules during interphase, to bipolar centrosomes associated with microtubules during prophase, to spindle fibers and spindle poles at metaphase and anaphase, to the midzone during early telophase, and to the midbody in late telophase in cells. Colocalizes with MDM2 in the nucleus By similarity. Isoform C: Nucleus By similarity. Note: Predominantly nuclear By similarity.
Sequence similarities	Contains 1 phorbol-ester/DAG-type zinc finger. Contains 1 Ras-associating domain. Contains 1 SARAH domain.

Ontologies

Keywords
Biological process	Cell cycle
Cellular component	Cytoplasm Cytoskeleton Microtubule Nucleus
Coding sequence diversity	Alternative promoter usage
Disease	Tumor suppressor
Domain	Zinc-finger
Ligand	Metal-binding Zinc
PTM	Acetylation Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	Ras protein signal transduction Inferred from sequence or structural similarity. Source: UniProtKB cell cycle arrest Inferred from sequence or structural similarity. Source: UniProtKB negative regulation of cell cycle arrest Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of protein ubiquitination Inferred from sequence or structural similarity. Source: UniProtKB protein stabilization Inferred from sequence or structural similarity. Source: UniProtKB response to DNA damage stimulus Inferred from sequence or structural similarity. Source: UniProtKB
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-KW microtubule Inferred from electronic annotation. Source: UniProtKB-KW microtubule cytoskeleton Inferred from sequence or structural similarity. Source: UniProtKB microtubule organizing center Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from sequence or structural similarity. Source: UniProtKB plasma membrane Inferred from electronic annotation. Source: Compara spindle pole Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative promoter usage. [Align] [Select]

Isoform A (identifier: Q99MK9-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform C (identifier: Q99MK9-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-70: Missing.
     71-119: FIWGVVRKGL...DSALERDTNV → MGEAETPSFE...SLVRRPRRDQ

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 340	339	Ras association domain-containing protein 1		PRO_0000068892
Regions
	Domain	194 – 288	95	Ras-associating
	Domain	290 – 337	48	SARAH
	Zinc finger	51 – 101	51	Phorbol-ester/DAG-type
	Region	2 – 115	114	Mediates interaction with E4F1 By similarity
	Region	311 – 314	4	MOAP1-binding By similarity
Amino acid modifications
	Modified residue	2	1	N-acetylserine By similarity
	Modified residue	2	1	Phosphoserine By similarity
Natural variations
	Alternative sequence	1 – 70	70	Missing in isoform C. Ref.2		VSP_050779
	Alternative sequence	71 – 119	49	FIWGV…RDTNV → MGEAETPSFEMTWSSTTSSG YCSQEDSDSELEQYFTARTS LVRRPRRDQ in isoform C. Ref.2		VSP_050780

Sequences

	Sequence		Length	Mass (Da)
	Isoform A [UniParc]. Last modified June 1, 2001. Version 1. Checksum: 7DEBA4AEE705757F	FASTA	340	38,789
	10 20 30 40 50 60 MSAEPELIEL RELAPSGRIG PGRTRLERAN ALRIAPGTTR NPSQQHVPGR GHRFQPAGPT 70 80 90 100 110 120 THTWCDLCGD FIWGVVRKGL QCAHCKFTCH YRCRALVCLD CCGPRDLGWD SALERDTNVD 130 140 150 160 170 180 EAVERETPDL SQAETEQKIK DYNGQINSNL FMSLNKDGSY TGFIKVQLKL VRPVSVPSSK 190 200 210 220 230 240 KPPSLQDARR GTGRSTAVKR RTSFYLPKDA IKHLHVLSRT RAREVIEALL RKFMVVDDPR 250 260 270 280 290 300 KFALFERTER HGQVYLRKLS DDEQPLKLRL LAGPSEKALS FVLKENDSGE VNWDAFSMPE 310 320 330 340 LHNFLRILQR EEEEHLRQIL QKYSRCRQKI QEALHACPLG
	Isoform C [UniParc]. Checksum: 6E11302FA93EC0BC	FASTA	270	31,193
	10 20 30 40 50 60 MGEAETPSFE MTWSSTTSSG YCSQEDSDSE LEQYFTARTS LVRRPRRDQD EAVERETPDL 70 80 90 100 110 120 SQAETEQKIK DYNGQINSNL FMSLNKDGSY TGFIKVQLKL VRPVSVPSSK KPPSLQDARR 130 140 150 160 170 180 GTGRSTAVKR RTSFYLPKDA IKHLHVLSRT RAREVIEALL RKFMVVDDPR KFALFERTER 190 200 210 220 230 240 HGQVYLRKLS DDEQPLKLRL LAGPSEKALS FVLKENDSGE VNWDAFSMPE LHNFLRILQR 250 260 270 EEEEHLRQIL QKYSRCRQKI QEALHACPLG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Epigenetic inactivation of RASSF1A in lung and breast cancers and malignant phenotype suppression." Burbee D.G., Forgacs E., Zochbauer-Muller S., Shivakumar L., Fong K., Gao B., Randle D., Kondo M., Virmani A., Bader S., Sekido Y., Latif F., Milchgrub S., Toyooka S., Gazdar A.F., Lerman M.I., Zabarovsky E., White M., Minna J.D. J. Natl. Cancer Inst. 93:691-699(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM C).
[2]	"A mouse locus containing the ortholog of the human RASSF1 tumor suppressor gene." Dammann R., Pfeifer G.P. Submitted (JAN-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], ALTERNATIVE PROMOTER USAGE. Strain: 129/Sv.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM C). Tissue: Mammary gland.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF132851 mRNA. Translation: AAD30061.1. AF333027 Genomic DNA. Translation: AAK21200.1. AF333027 Genomic DNA. Translation: AAK21201.1. BC002173 mRNA. Translation: AAH02173.1.
IPI	IPI00117796. IPI00652016.
RefSeq	NP_001230677.1. NM_001243748.1. NP_062687.1. NM_019713.4.
UniGene	Mm.12091. Mm.488708.
3D structure databases
ProteinModelPortal	Q99MK9.
SMR	Q99MK9. Positions 49-101, 134-286.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q99MK9. 8 interactions.
MINT	MINT-5197358.
PTM databases
PhosphoSite	Q99MK9.
Proteomic databases
PRIDE	Q99MK9.
Protocols and materials databases
DNASU	56289.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000010211; ENSMUSP00000010211; ENSMUSG00000010067. ENSMUST00000093786; ENSMUSP00000091301; ENSMUSG00000010067.
GeneID	56289.
KEGG	mmu:56289.
Organism-specific databases
CTD	11186.
MGI	MGI:1928386. Rassf1.
Phylogenomic databases
eggNOG	NOG308852.
GeneTree	ENSGT00390000003367.
HOGENOM	HOG000013025.
HOVERGEN	HBG054362.
KO	K09850.
OMA	PRRDQDE.
OrthoDB	EOG4V4381.
Gene expression databases
ArrayExpress	Q99MK9.
Bgee	Q99MK9.
Genevestigator	Q99MK9.
GermOnline	ENSMUSG00000010067. Mus musculus.
Family and domain databases
InterPro	IPR002219. Prot_Kinase_C-like_PE/DAG-bd. IPR000159. Ras-assoc. IPR011524. SARAH_dom. [Graphical view]
Pfam	PF00130. C1_1. 1 hit. PF00788. RA. 1 hit. [Graphical view]
SMART	SM00109. C1. 1 hit. SM00314. RA. 1 hit. [Graphical view]
PROSITE	PS50200. RA. 1 hit. PS50951. SARAH. 1 hit. PS00479. ZF_DAG_PE_1. 1 hit. PS50081. ZF_DAG_PE_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	RASSF1. mouse.
NextBio	312192.
SOURCE	Search...

Entry information

Entry name

RASF1_MOUSE

Accession

Primary (citable) accession number: Q99MK9
Secondary accession number(s): Q9WUF5

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 19, 2004
Last sequence update:	June 1, 2001
Last modified:	May 1, 2013
This is version 94 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program