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Q99MK9 (RASF1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 103. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ras association domain-containing protein 1
Alternative name(s):
Protein 123F2
Gene names
Name:Rassf1
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length340 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Potential tumor suppressor. Required for death receptor-dependent apoptosis. Mediates activation of Mediates activation of STK3/MST2 and STK4/MST1 during Fas-induced apoptosis by preventing their dephosphorylation. When associated with MOAP1, promotes BAX conformational change and translocation to mitochondrial membranes in response to TNF and TNFSF10 stimulation. Isoform A interacts with CDC20, an activator of the anaphase-promoting complex, APC, resulting in the inhibition of APC activity and mitotic progression. Inhibits proliferation by negatively regulating cell cycle progression at the level of G1/S-phase transition by regulating accumulation of cyclin D1 protein. Isoform C has been shown not to perform these roles, no function has been identified for this isoform. Isoform A disrupts interactions among MDM2, DAXX and USP7, thus contributing to the efficient activation of TP53 by promoting MDM2 self-ubiquitination in cell-cycle checkpoint control in response to DNA damage By similarity.

Subunit structure

Interacts with MAP1S and XPA. Binds to the N-terminal of CDC20 during prometaphase. Binds to STK3/MST2 and STK4/MST1. Isoform A interacts with MOAP1 and E4F1. Recruited to the TNFRSF1A and TNFRSF10A complexes in response to their respective cognate ligand, after internalization. Can self-associate. Isoform A interacts with RSSF5 and probably associates with HRAS via a RSSF1 isoform A-RSSF5heterodimer. Part of a complex with MDM2, DAXX, RASSF1 and USP7. Isoform A interacts (via C-terminus) with DAXX (via N-terminus); the interaction is independent of MDM2 and TP53. Isoform A interacts (via N-terminus) with MDM2 (via C-terminus); the interaction is independent of TP53 By similarity.

Subcellular location

Isoform A: Cytoplasmcytoskeleton By similarity. Cytoplasmcytoskeletonmicrotubule organizing centercentrosome By similarity. Cytoplasmcytoskeletonspindle By similarity. Cytoplasmcytoskeletonspindle pole By similarity. Note: Localizes to cytoplasmic microtubules during interphase, to bipolar centrosomes associated with microtubules during prophase, to spindle fibers and spindle poles at metaphase and anaphase, to the midzone during early telophase, and to the midbody in late telophase in cells. Colocalizes with MDM2 in the nucleus By similarity.

Isoform C: Nucleus By similarity. Note: Predominantly nuclear By similarity.

Sequence similarities

Contains 1 phorbol-ester/DAG-type zinc finger.

Contains 1 Ras-associating domain.

Contains 1 SARAH domain.

Ontologies

Keywords
   Biological processCell cycle
   Cellular componentCytoplasm
Cytoskeleton
Microtubule
Nucleus
   Coding sequence diversityAlternative promoter usage
   DiseaseTumor suppressor
   DomainZinc-finger
   LigandMetal-binding
Zinc
   PTMAcetylation
Phosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processRas protein signal transduction

Inferred from sequence or structural similarity. Source: UniProtKB

cell cycle arrest

Inferred from sequence or structural similarity. Source: UniProtKB

cellular response to DNA damage stimulus

Inferred from sequence or structural similarity. Source: UniProtKB

negative regulation of cell cycle arrest

Inferred from sequence or structural similarity. Source: UniProtKB

positive regulation of protein ubiquitination

Inferred from sequence or structural similarity. Source: UniProtKB

protein stabilization

Inferred from sequence or structural similarity. Source: UniProtKB

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-KW

microtubule

Inferred from electronic annotation. Source: UniProtKB-KW

microtubule cytoskeleton

Inferred from sequence or structural similarity. Source: UniProtKB

microtubule organizing center

Inferred from electronic annotation. Source: UniProtKB-SubCell

nucleus

Inferred from sequence or structural similarity. Source: UniProtKB

plasma membrane

Inferred from electronic annotation. Source: Ensembl

spindle pole

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionmetal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative promoter usage. [Align] [Select]
Isoform A (identifier: Q99MK9-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform C (identifier: Q99MK9-2)

The sequence of this isoform differs from the canonical sequence as follows:
     2-70: Missing.
     71-119: FIWGVVRKGL...DSALERDTNV → MGEAETPSFE...SLVRRPRRDQ

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 340339Ras association domain-containing protein 1
PRO_0000068892

Regions

Domain194 – 28895Ras-associating
Domain290 – 33748SARAH
Zinc finger51 – 10151Phorbol-ester/DAG-type
Region2 – 115114Mediates interaction with E4F1 By similarity
Region311 – 3144MOAP1-binding By similarity

Amino acid modifications

Modified residue21N-acetylserine By similarity
Modified residue21Phosphoserine By similarity

Natural variations

Alternative sequence2 – 7069Missing in isoform C.
VSP_050779
Alternative sequence71 – 11949FIWGV…RDTNV → MGEAETPSFEMTWSSTTSSG YCSQEDSDSELEQYFTARTS LVRRPRRDQ in isoform C. Ref.2
VSP_050780

Sequences

Sequence LengthMass (Da)Tools
Isoform A [UniParc].

Last modified June 1, 2001. Version 1.
Checksum: 7DEBA4AEE705757F

FASTA34038,789
        10         20         30         40         50         60 
MSAEPELIEL RELAPSGRIG PGRTRLERAN ALRIAPGTTR NPSQQHVPGR GHRFQPAGPT 

        70         80         90        100        110        120 
THTWCDLCGD FIWGVVRKGL QCAHCKFTCH YRCRALVCLD CCGPRDLGWD SALERDTNVD 

       130        140        150        160        170        180 
EAVERETPDL SQAETEQKIK DYNGQINSNL FMSLNKDGSY TGFIKVQLKL VRPVSVPSSK 

       190        200        210        220        230        240 
KPPSLQDARR GTGRSTAVKR RTSFYLPKDA IKHLHVLSRT RAREVIEALL RKFMVVDDPR 

       250        260        270        280        290        300 
KFALFERTER HGQVYLRKLS DDEQPLKLRL LAGPSEKALS FVLKENDSGE VNWDAFSMPE 

       310        320        330        340 
LHNFLRILQR EEEEHLRQIL QKYSRCRQKI QEALHACPLG 

« Hide

Isoform C [UniParc].

Checksum: A7E1074A043ED651
Show »

FASTA27131,324

References

« Hide 'large scale' references
[1]"Epigenetic inactivation of RASSF1A in lung and breast cancers and malignant phenotype suppression."
Burbee D.G., Forgacs E., Zochbauer-Muller S., Shivakumar L., Fong K., Gao B., Randle D., Kondo M., Virmani A., Bader S., Sekido Y., Latif F., Milchgrub S., Toyooka S., Gazdar A.F., Lerman M.I., Zabarovsky E., White M., Minna J.D.
J. Natl. Cancer Inst. 93:691-699(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM C).
[2]"A mouse locus containing the ortholog of the human RASSF1 tumor suppressor gene."
Dammann R., Pfeifer G.P.
Submitted (JAN-2001) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], ALTERNATIVE PROMOTER USAGE.
Strain: 129/Sv.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM C).
Tissue: Mammary gland.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF132851 mRNA. Translation: AAD30061.1.
AF333027 Genomic DNA. Translation: AAK21200.1.
AF333027 Genomic DNA. Translation: AAK21201.1.
BC002173 mRNA. Translation: AAH02173.1.
RefSeqNP_001230677.1. NM_001243748.1.
NP_062687.1. NM_019713.4.
UniGeneMm.12091.
Mm.488708.

3D structure databases

ProteinModelPortalQ99MK9.
SMRQ99MK9. Positions 49-101, 134-286, 290-334.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid207877. 11 interactions.
IntActQ99MK9. 10 interactions.
MINTMINT-5197358.

PTM databases

PhosphoSiteQ99MK9.

Proteomic databases

PRIDEQ99MK9.

Protocols and materials databases

DNASU56289.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000010211; ENSMUSP00000010211; ENSMUSG00000010067.
ENSMUST00000093786; ENSMUSP00000091301; ENSMUSG00000010067. [Q99MK9-1]
GeneID56289.
KEGGmmu:56289.
UCSCuc012hag.2. mouse. [Q99MK9-1]

Organism-specific databases

CTD11186.
MGIMGI:1928386. Rassf1.

Phylogenomic databases

eggNOGNOG308852.
GeneTreeENSGT00390000003367.
HOGENOMHOG000013025.
HOVERGENHBG054362.
KOK09850.
OMATTSSGYC.
OrthoDBEOG7TF796.
PhylomeDBQ99MK9.
TreeFamTF319243.

Gene expression databases

ArrayExpressQ99MK9.
BgeeQ99MK9.
GenevestigatorQ99MK9.

Family and domain databases

InterProIPR002219. Prot_Kinase_C-like_PE/DAG-bd.
IPR000159. Ras-assoc.
IPR011524. SARAH_dom.
[Graphical view]
PfamPF00130. C1_1. 1 hit.
PF00788. RA. 1 hit.
[Graphical view]
SMARTSM00109. C1. 1 hit.
SM00314. RA. 1 hit.
[Graphical view]
PROSITEPS50200. RA. 1 hit.
PS50951. SARAH. 1 hit.
PS00479. ZF_DAG_PE_1. 1 hit.
PS50081. ZF_DAG_PE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSRASSF1. mouse.
NextBio312192.
PROQ99MK9.
SOURCESearch...

Entry information

Entry nameRASF1_MOUSE
AccessionPrimary (citable) accession number: Q99MK9
Secondary accession number(s): Q9WUF5
Entry history
Integrated into UniProtKB/Swiss-Prot: July 19, 2004
Last sequence update: June 1, 2001
Last modified: April 16, 2014
This is version 103 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot