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Q99MK8 (ARBK1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 113. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Beta-adrenergic receptor kinase 1

Short name=Beta-ARK-1
EC=2.7.11.15
Alternative name(s):
G-protein-coupled receptor kinase 2
Gene names
Name:Adrbk1
Synonyms:Grk2
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length689 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Specifically phosphorylates the agonist-occupied form of the beta-adrenergic and closely related receptors, probably inducing a desensitization of them.

Catalytic activity

ATP + [beta-adrenergic receptor] = ADP + [beta-adrenergic receptor] phosphate.

Subunit structure

Interacts with GIT1. Interacts with, and phosphorylates chemokine-stimulated CCR5. Interacts with ARRB1 By similarity.

Sequence similarities

Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. GPRK subfamily.

Contains 1 AGC-kinase C-terminal domain.

Contains 1 PH domain.

Contains 1 protein kinase domain.

Contains 1 RGS domain.

Ontologies

Keywords
   LigandATP-binding
Nucleotide-binding
   Molecular functionKinase
Serine/threonine-protein kinase
Transferase
   PTMPhosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processG-protein coupled receptor signaling pathway

Inferred from direct assay PubMed 14712229. Source: MGI

desensitization of G-protein coupled receptor protein signaling pathway

Inferred from mutant phenotype PubMed 10338466. Source: MGI

heart development

Inferred from mutant phenotype PubMed 8917529. Source: MGI

peptidyl-serine phosphorylation

Inferred from direct assay PubMed 17544362. Source: MGI

peptidyl-threonine phosphorylation

Inferred from direct assay PubMed 17544362. Source: MGI

protein phosphorylation

Inferred from direct assay PubMed 15218143. Source: MGI

regulation of the force of heart contraction

Inferred from genetic interaction PubMed 7761854. Source: MGI

termination of G-protein coupled receptor signaling pathway

Inferred from electronic annotation. Source: InterPro

   Cellular_componentmembrane

Inferred from direct assay PubMed 15218143. Source: MGI

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

G-protein coupled receptor kinase activity

Inferred from direct assay PubMed 14712229PubMed 1869533. Source: MGI

beta-adrenergic receptor kinase activity

Inferred from electronic annotation. Source: UniProtKB-EC

protein kinase activity

Inferred from direct assay PubMed 15218143. Source: MGI

protein serine/threonine kinase activity

Inferred from direct assay PubMed 17544362. Source: MGI

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 689689Beta-adrenergic receptor kinase 1
PRO_0000085629

Regions

Domain54 – 175122RGS
Domain191 – 453263Protein kinase
Domain454 – 52168AGC-kinase C-terminal
Domain558 – 65295PH
Nucleotide binding197 – 2059ATP By similarity
Region1 – 190190N-terminal

Sites

Active site3171Proton acceptor By similarity
Binding site2201ATP By similarity

Amino acid modifications

Modified residue6701Phosphoserine By similarity

Experimental info

Sequence conflict1601D → N in AAK21896. Ref.1
Sequence conflict3741W → C in AAK21896. Ref.1
Sequence conflict5201E → V in AAK21896. Ref.1
Sequence conflict6001L → F in AAK21896. Ref.1
Sequence conflict6791P → R in AAK21896. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q99MK8 [UniParc].

Last modified May 24, 2004. Version 2.
Checksum: 508F32B287DD99C6

FASTA68979,639
        10         20         30         40         50         60 
MADLEAVLAD VSYLMAMEKS KATPAARASK KILLPEPSIR SVMQKYLEDR GEVTFEKIFS 

        70         80         90        100        110        120 
QKLGYLLFRD FCLNHLEEAK PLVEFYEEIK KYEKLETEEE RVVRSREIFD SYIMKELLAC 

       130        140        150        160        170        180 
SHPFSKNATE HVQGHLVKKQ VPPDLFQPYI EEICQNLRGD VFQKFIESDK FTRFCQWKNV 

       190        200        210        220        230        240 
ELNIHLTMND FSVHRIIGRG GFGEVYGCRK ADTGKMYAMK CLDKKRIKMK QGETLALNER 

       250        260        270        280        290        300 
IMLSLVSTGD CPFIVCMSYA FHTPDKLSFI LDLMNGGDLH YHLSQHGVFS EADMRFYAAE 

       310        320        330        340        350        360 
IILGLEHMHN RFVVYRDLKP ANILLDEHGH VRISDLGLAC DFSKKRPHAS VGTHGYMAPE 

       370        380        390        400        410        420 
VLQKGVAYDS SADWFSLGCM LFKLLRGHSP FRQHKTKDKH EIDRMTLTMA VELPDSFSPE 

       430        440        450        460        470        480 
LRSLLEGLLQ RDVNRRLGCL GRGAQEVKES PFFRSLDWQM VFLQKYPPPL IPPRGEVNAA 

       490        500        510        520        530        540 
DAFDIGSFDE EDTKGIKLLD SDQELYRNFP LTISERWQQE VAETVFDTIN AETDRLEARK 

       550        560        570        580        590        600 
KAKNKQLGHE EDYALGKDCI VHGYMSKMGN PFLTQWQRRY FYLFPNRLEW RGEGEAPQSL 

       610        620        630        640        650        660 
LTMEEIQSVE ETQIKERKCL LLKIRGGKQF VLQCDSDPEL VQWKKELRDA YREAQQLVQR 

       670        680 
VPKMKNKPRS PVVELSKVPL IQRGSANGL 

« Hide

References

« Hide 'large scale' references
[1]"Cloning of GRK2 cDNA from S49 murine lymphoma cells."
Hughes R.J., Anderson K.L., Kiel D., Insel P.A.
Am. J. Physiol. 270:C885-C891(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Lymphoma.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 3-689.
[3]"Comprehensive identification of phosphorylation sites in postsynaptic density preparations."
Trinidad J.C., Specht C.G., Thalhammer A., Schoepfer R., Burlingame A.L.
Mol. Cell. Proteomics 5:914-922(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Brain.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF333028 mRNA. Translation: AAK21896.1.
BC003196 mRNA. Translation: AAH03196.1.
RefSeqXP_006531696.1. XM_006531633.1.
UniGeneMm.254144.

3D structure databases

ProteinModelPortalQ99MK8.
SMRQ99MK8. Positions 30-670.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

DIPDIP-32413N.
IntActQ99MK8. 4 interactions.
MINTMINT-4088166.

PTM databases

PhosphoSiteQ99MK8.

Proteomic databases

PaxDbQ99MK8.
PRIDEQ99MK8.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000088737; ENSMUSP00000086114; ENSMUSG00000024858.
GeneID110355.
UCSCuc008fzu.1. mouse.

Organism-specific databases

MGIMGI:87940. Adrbk1.

Phylogenomic databases

eggNOGCOG0515.
GeneTreeENSGT00730000110751.
HOGENOMHOG000006742.
HOVERGENHBG050559.
InParanoidQ99MK8.
OMADFCLKHL.
PhylomeDBQ99MK8.

Gene expression databases

ArrayExpressQ99MK8.
BgeeQ99MK8.
CleanExMM_ADRBK1.
GenevestigatorQ99MK8.

Family and domain databases

Gene3D2.30.29.30. 1 hit.
InterProIPR000961. AGC-kinase_C.
IPR000239. GPCR_kinase.
IPR011009. Kinase-like_dom.
IPR011993. PH_like_dom.
IPR001849. Pleckstrin_homology.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR016137. Regulat_G_prot_signal_superfam.
IPR000342. RGS_dom.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamPF00169. PH. 1 hit.
PF00069. Pkinase. 1 hit.
PF00615. RGS. 1 hit.
[Graphical view]
PRINTSPR00717. GPCRKINASE.
SMARTSM00233. PH. 1 hit.
SM00315. RGS. 1 hit.
SM00133. S_TK_X. 1 hit.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMSSF48097. SSF48097. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEPS51285. AGC_KINASE_CTER. 1 hit.
PS50003. PH_DOMAIN. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
PS50132. RGS. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

PROQ99MK8.
SOURCESearch...

Entry information

Entry nameARBK1_MOUSE
AccessionPrimary (citable) accession number: Q99MK8
Secondary accession number(s): Q99LL8
Entry history
Integrated into UniProtKB/Swiss-Prot: May 24, 2004
Last sequence update: May 24, 2004
Last modified: April 16, 2014
This is version 113 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Human and mouse protein kinases

Human and mouse protein kinases: classification and index

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot