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Q99MK8

- ARBK1_MOUSE

UniProt

Q99MK8 - ARBK1_MOUSE

Protein

Beta-adrenergic receptor kinase 1

Gene

Adrbk1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 118 (01 Oct 2014)
      Sequence version 2 (24 May 2004)
      Previous versions | rss
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    Functioni

    Specifically phosphorylates the agonist-occupied form of the beta-adrenergic and closely related receptors, probably inducing a desensitization of them.

    Catalytic activityi

    ATP + [beta-adrenergic receptor] = ADP + [beta-adrenergic receptor] phosphate.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei220 – 2201ATPPROSITE-ProRule annotation
    Active sitei317 – 3171Proton acceptorPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi197 – 2059ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. beta-adrenergic receptor kinase activity Source: UniProtKB-EC
    3. G-protein coupled receptor kinase activity Source: MGI
    4. protein binding Source: MGI
    5. protein kinase activity Source: MGI
    6. protein serine/threonine kinase activity Source: MGI

    GO - Biological processi

    1. desensitization of G-protein coupled receptor protein signaling pathway Source: MGI
    2. G-protein coupled receptor signaling pathway Source: MGI
    3. heart development Source: MGI
    4. peptidyl-serine phosphorylation Source: MGI
    5. peptidyl-threonine phosphorylation Source: MGI
    6. protein phosphorylation Source: MGI
    7. regulation of the force of heart contraction Source: MGI
    8. termination of G-protein coupled receptor signaling pathway Source: InterPro

    Keywords - Molecular functioni

    Kinase, Serine/threonine-protein kinase, Transferase

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    ReactomeiREACT_207651. G alpha (q) signalling events.
    REACT_215587. Calmodulin induced events.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Beta-adrenergic receptor kinase 1 (EC:2.7.11.15)
    Short name:
    Beta-ARK-1
    Alternative name(s):
    G-protein-coupled receptor kinase 2
    Gene namesi
    Name:Adrbk1
    Synonyms:Grk2
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 19

    Organism-specific databases

    MGIiMGI:87940. Adrbk1.

    Subcellular locationi

    GO - Cellular componenti

    1. membrane Source: MGI

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 689689Beta-adrenergic receptor kinase 1PRO_0000085629Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei670 – 6701PhosphoserineBy similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiQ99MK8.
    PaxDbiQ99MK8.
    PRIDEiQ99MK8.

    PTM databases

    PhosphoSiteiQ99MK8.

    Expressioni

    Gene expression databases

    ArrayExpressiQ99MK8.
    BgeeiQ99MK8.
    CleanExiMM_ADRBK1.
    GenevestigatoriQ99MK8.

    Interactioni

    Subunit structurei

    Interacts with GIT1. Interacts with, and phosphorylates chemokine-stimulated CCR5. Interacts with ARRB1 By similarity.By similarity

    Protein-protein interaction databases

    DIPiDIP-32413N.
    IntActiQ99MK8. 4 interactions.
    MINTiMINT-4088166.

    Structurei

    3D structure databases

    ProteinModelPortaliQ99MK8.
    SMRiQ99MK8. Positions 30-668.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini54 – 175122RGSPROSITE-ProRule annotationAdd
    BLAST
    Domaini191 – 453263Protein kinasePROSITE-ProRule annotationAdd
    BLAST
    Domaini454 – 52168AGC-kinase C-terminalAdd
    BLAST
    Domaini558 – 65295PHPROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 190190N-terminalAdd
    BLAST

    Sequence similaritiesi

    Contains 1 AGC-kinase C-terminal domain.Curated
    Contains 1 PH domain.PROSITE-ProRule annotation
    Contains 1 protein kinase domain.PROSITE-ProRule annotation
    Contains 1 RGS domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0515.
    GeneTreeiENSGT00730000110751.
    HOGENOMiHOG000006742.
    HOVERGENiHBG050559.
    InParanoidiQ99MK8.
    OMAiDFCLKHL.
    PhylomeDBiQ99MK8.

    Family and domain databases

    Gene3Di2.30.29.30. 1 hit.
    InterProiIPR000961. AGC-kinase_C.
    IPR000239. GPCR_kinase.
    IPR011009. Kinase-like_dom.
    IPR001849. PH_domain.
    IPR011993. PH_like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR016137. Regulat_G_prot_signal_superfam.
    IPR000342. RGS_dom.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view]
    PfamiPF00169. PH. 1 hit.
    PF00069. Pkinase. 1 hit.
    PF00615. RGS. 1 hit.
    [Graphical view]
    PRINTSiPR00717. GPCRKINASE.
    SMARTiSM00233. PH. 1 hit.
    SM00315. RGS. 1 hit.
    SM00133. S_TK_X. 1 hit.
    SM00220. S_TKc. 1 hit.
    [Graphical view]
    SUPFAMiSSF48097. SSF48097. 1 hit.
    SSF56112. SSF56112. 1 hit.
    PROSITEiPS51285. AGC_KINASE_CTER. 1 hit.
    PS50003. PH_DOMAIN. 1 hit.
    PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    PS50132. RGS. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q99MK8-1 [UniParc]FASTAAdd to Basket

    « Hide

    MADLEAVLAD VSYLMAMEKS KATPAARASK KILLPEPSIR SVMQKYLEDR    50
    GEVTFEKIFS QKLGYLLFRD FCLNHLEEAK PLVEFYEEIK KYEKLETEEE 100
    RVVRSREIFD SYIMKELLAC SHPFSKNATE HVQGHLVKKQ VPPDLFQPYI 150
    EEICQNLRGD VFQKFIESDK FTRFCQWKNV ELNIHLTMND FSVHRIIGRG 200
    GFGEVYGCRK ADTGKMYAMK CLDKKRIKMK QGETLALNER IMLSLVSTGD 250
    CPFIVCMSYA FHTPDKLSFI LDLMNGGDLH YHLSQHGVFS EADMRFYAAE 300
    IILGLEHMHN RFVVYRDLKP ANILLDEHGH VRISDLGLAC DFSKKRPHAS 350
    VGTHGYMAPE VLQKGVAYDS SADWFSLGCM LFKLLRGHSP FRQHKTKDKH 400
    EIDRMTLTMA VELPDSFSPE LRSLLEGLLQ RDVNRRLGCL GRGAQEVKES 450
    PFFRSLDWQM VFLQKYPPPL IPPRGEVNAA DAFDIGSFDE EDTKGIKLLD 500
    SDQELYRNFP LTISERWQQE VAETVFDTIN AETDRLEARK KAKNKQLGHE 550
    EDYALGKDCI VHGYMSKMGN PFLTQWQRRY FYLFPNRLEW RGEGEAPQSL 600
    LTMEEIQSVE ETQIKERKCL LLKIRGGKQF VLQCDSDPEL VQWKKELRDA 650
    YREAQQLVQR VPKMKNKPRS PVVELSKVPL IQRGSANGL 689
    Length:689
    Mass (Da):79,639
    Last modified:May 24, 2004 - v2
    Checksum:i508F32B287DD99C6
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti160 – 1601D → N in AAK21896. (PubMed:8638670)Curated
    Sequence conflicti374 – 3741W → C in AAK21896. (PubMed:8638670)Curated
    Sequence conflicti520 – 5201E → V in AAK21896. (PubMed:8638670)Curated
    Sequence conflicti600 – 6001L → F in AAK21896. (PubMed:8638670)Curated
    Sequence conflicti679 – 6791P → R in AAK21896. (PubMed:8638670)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF333028 mRNA. Translation: AAK21896.1.
    BC003196 mRNA. Translation: AAH03196.1.
    CCDSiCCDS70915.1.
    RefSeqiNP_001277747.1. NM_001290818.1.
    UniGeneiMm.254144.

    Genome annotation databases

    EnsembliENSMUST00000088737; ENSMUSP00000086114; ENSMUSG00000024858.
    GeneIDi110355.
    UCSCiuc008fzu.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF333028 mRNA. Translation: AAK21896.1 .
    BC003196 mRNA. Translation: AAH03196.1 .
    CCDSi CCDS70915.1.
    RefSeqi NP_001277747.1. NM_001290818.1.
    UniGenei Mm.254144.

    3D structure databases

    ProteinModelPortali Q99MK8.
    SMRi Q99MK8. Positions 30-668.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-32413N.
    IntActi Q99MK8. 4 interactions.
    MINTi MINT-4088166.

    PTM databases

    PhosphoSitei Q99MK8.

    Proteomic databases

    MaxQBi Q99MK8.
    PaxDbi Q99MK8.
    PRIDEi Q99MK8.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000088737 ; ENSMUSP00000086114 ; ENSMUSG00000024858 .
    GeneIDi 110355.
    UCSCi uc008fzu.1. mouse.

    Organism-specific databases

    MGIi MGI:87940. Adrbk1.

    Phylogenomic databases

    eggNOGi COG0515.
    GeneTreei ENSGT00730000110751.
    HOGENOMi HOG000006742.
    HOVERGENi HBG050559.
    InParanoidi Q99MK8.
    OMAi DFCLKHL.
    PhylomeDBi Q99MK8.

    Enzyme and pathway databases

    Reactomei REACT_207651. G alpha (q) signalling events.
    REACT_215587. Calmodulin induced events.

    Miscellaneous databases

    PROi Q99MK8.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q99MK8.
    Bgeei Q99MK8.
    CleanExi MM_ADRBK1.
    Genevestigatori Q99MK8.

    Family and domain databases

    Gene3Di 2.30.29.30. 1 hit.
    InterProi IPR000961. AGC-kinase_C.
    IPR000239. GPCR_kinase.
    IPR011009. Kinase-like_dom.
    IPR001849. PH_domain.
    IPR011993. PH_like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR016137. Regulat_G_prot_signal_superfam.
    IPR000342. RGS_dom.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view ]
    Pfami PF00169. PH. 1 hit.
    PF00069. Pkinase. 1 hit.
    PF00615. RGS. 1 hit.
    [Graphical view ]
    PRINTSi PR00717. GPCRKINASE.
    SMARTi SM00233. PH. 1 hit.
    SM00315. RGS. 1 hit.
    SM00133. S_TK_X. 1 hit.
    SM00220. S_TKc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF48097. SSF48097. 1 hit.
    SSF56112. SSF56112. 1 hit.
    PROSITEi PS51285. AGC_KINASE_CTER. 1 hit.
    PS50003. PH_DOMAIN. 1 hit.
    PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    PS50132. RGS. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning of GRK2 cDNA from S49 murine lymphoma cells."
      Hughes R.J., Anderson K.L., Kiel D., Insel P.A.
      Am. J. Physiol. 270:C885-C891(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Lymphoma.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 3-689.
    3. "Comprehensive identification of phosphorylation sites in postsynaptic density preparations."
      Trinidad J.C., Specht C.G., Thalhammer A., Schoepfer R., Burlingame A.L.
      Mol. Cell. Proteomics 5:914-922(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Brain.

    Entry informationi

    Entry nameiARBK1_MOUSE
    AccessioniPrimary (citable) accession number: Q99MK8
    Secondary accession number(s): Q99LL8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 24, 2004
    Last sequence update: May 24, 2004
    Last modified: October 1, 2014
    This is version 118 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. Human and mouse protein kinases
      Human and mouse protein kinases: classification and index
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3