Q99MK2 (KAT5_RAT) Reviewed, UniProtKB/Swiss-Prot
Last modified April 16, 2014. Version 98. History...
Names and origin
|Protein names||Recommended name:|
Histone acetyltransferase KAT5
60 kDa Tat-interactive protein
Histone acetyltransferase HTATIP
Lysine acetyltransferase 5
|Organism||Rattus norvegicus (Rat) [Reference proteome]|
|Taxonomic identifier||10116 [NCBI]|
|Taxonomic lineage||Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus|
|Sequence length||513 AA.|
|Protein existence||Evidence at protein level|
General annotation (Comments)
Catalytic subunit of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. The NuA4 complex ATPase and helicase activities seem to be, at least in part, contributed by the association of RUVBL1 and RUVBL2 with EP400. NuA4 may also play a direct role in DNA repair when recruited to sites of DNA damage. Directly acetylates and activates ATM. Relieves NR1D2-mediated inhibition of APOC3 expression by acetylating NR1D2. Component of a SWR1-like complex that specifically mediates the removal of histone H2A.Z/H2AFZ from the nucleosome By similarity.
Acetyl-CoA + [histone] = CoA + acetyl-[histone].
Component of the NuA4 histone acetyltransferase complex which contains the catalytic subunit KAT5/TIP60 and the subunits EP400, TRRAP/PAF400, BRD8/SMAP, EPC1, DMAP1/DNMAP1, RUVBL1/TIP49, RUVBL2, ING3, actin, ACTL6A/BAF53A, MORF4L1/MRG15, MORF4L2/MRGX, MRGBP, YEATS4/GAS41, VPS72/YL1 and MEAF6 By similarity. HTATTIP/TIP60, EPC1, and ING3 together constitute a minimal HAT complex termed Piccolo NuA4 By similarity. The NuA4 complex interacts with MYC By similarity. Interacts with ATM By similarity. Interacts with JADE1 By similarity. Interacts with PLA2G4A/CPLA2, EDNRA and HDAC7 By similarity. Interacts with the cytoplasmic tail of APP and APBB1/FE65. Interacts with TRIM24 and TRIM68 By similarity. Forms a complex with SENP6 and UBE2I in response to UV irradiation. Identified in a complex with HINT1 By similarity. Interacts with ATF2 and CUL3. Interacts with NR1D2 (via N-terminus). Component of a SWR1-like complex By similarity. Ref.2 Ref.3
Nucleus By similarity. Nucleus › nucleolus By similarity. Cytoplasm › perinuclear region By similarity. Note: Upon stimulation with EDN1, it is exported from the nucleus to the perinuclear region By similarity.
Sumoylated by UBE2I at Lys-430 and Lys-451, leading to increase of its histone acetyltransferase activity in UV-induced DNA damage response, as well as its translocation to nuclear bodies By similarity.
Phosphorylated. Phosphorylated form has a higher activity By similarity.
Ubiquitinated by MDM2, leading to its proteasome-dependent degradation By similarity.
Autoacetylation at Lys-327 is required for proper function By similarity.
Belongs to the MYST (SAS/MOZ) family.
Contains 1 C2HC-type zinc finger.
|This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]|
|Isoform 1 (identifier: Q99MK2-1) |
This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
|Isoform 2 (identifier: Q99MK2-2) |
The sequence of this isoform differs from the canonical sequence as follows:
Sequence annotation (Features)
|Feature key||Position(s)||Length||Description||Graphical view||Feature identifier|
|Chain||1 – 513||513||Histone acetyltransferase KAT5||PRO_0000051582|
|Zinc finger||261 – 283||23||C2HC-type|
|Region||368 – 513||146||Interaction with ATF2 By similarity|
|Region||377 – 383||7||Acetyl-CoA binding By similarity|
|Active site||327||1||By similarity|
|Active site||369||1||Nucleophile By similarity|
|Binding site||372||1||Acetyl-CoA By similarity|
|Binding site||407||1||Acetyl-CoA By similarity|
Amino acid modifications
|Modified residue||52||1||N6-acetyllysine By similarity|
|Modified residue||86||1||Phosphoserine By similarity|
|Modified residue||90||1||Phosphoserine By similarity|
|Modified residue||327||1||N6-acetyllysine; by autocatalysis By similarity|
|Cross-link||430||Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO) By similarity|
|Cross-link||451||Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO) By similarity|
|Alternative sequence||96 – 147||52||Missing in isoform 2.||VSP_019781|
|||"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."|
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
|||"A transcriptionally active complex of APP with Fe65 and histone acetyltransferase Tip60."|
Cao X., Suedhof T.C.
Science 293:115-120(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 63-506 (ISOFORM 2), INTERACTION WITH APP.
|||"Fe65 is required for Tip60-directed histone H4 acetylation at DNA strand breaks."|
Stante M., Minopoli G., Passaro F., Raia M., Vecchio L.D., Russo T.
Proc. Natl. Acad. Sci. U.S.A. 106:5093-5098(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH APBB1.
|+||Additional computationally mapped references.|
|BC083879 mRNA. Translation: AAH83879.1.|
AF333984 mRNA. Translation: AAK20836.1.
|RefSeq||NP_001005872.1. NM_001005872.1. |
3D structure databases
|SMR||Q99MK2. Positions 11-79, 230-504. |
Protein-protein interaction databases
|BioGrid||251357. 1 interaction.|
Protocols and materials databases
Genome annotation databases
|Ensembl||ENSRNOT00000028107; ENSRNOP00000028107; ENSRNOG00000020707. [Q99MK2-2]|
ENSRNOT00000041916; ENSRNOP00000039632; ENSRNOG00000020707. [Q99MK2-1]
|UCSC||RGD:621061. rat. [Q99MK2-1]|
|RGD||621061. Kat5. |
Gene expression databases
Family and domain databases
|Gene3D||3.40.630.30. 1 hit. |
|InterPro||IPR016181. Acyl_CoA_acyltransferase. |
|Pfam||PF01853. MOZ_SAS. 1 hit. |
PF11717. Tudor-knot. 1 hit.
|SMART||SM00298. CHROMO. 1 hit. |
|SUPFAM||SSF54160. SSF54160. 1 hit. |
SSF55729. SSF55729. 1 hit.
|Accession||Primary (citable) accession number: Q99MK2|
Secondary accession number(s): Q5XI16
|Entry status||Reviewed (UniProtKB/Swiss-Prot)|
|Annotation program||Chordata Protein Annotation Program|
Index of protein domains and families