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Protein

ATP-dependent RNA helicase DDX50

Gene

Ddx50

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi178 – 1858ATPPROSITE-ProRule annotation

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Helicase, Hydrolase

Keywords - Ligandi

ATP-binding, Nucleotide-binding, RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent RNA helicase DDX50 (EC:3.6.4.13)
Alternative name(s):
DEAD box protein 50
Gu-beta
Nucleolar protein Gu2
Gene namesi
Name:Ddx50
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 10

Organism-specific databases

MGIiMGI:2182303. Ddx50.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 734734ATP-dependent RNA helicase DDX50PRO_0000055055Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei41 – 411PhosphoserineBy similarity
Modified residuei81 – 811PhosphoserineBy similarity
Modified residuei85 – 851PhosphoserineBy similarity
Modified residuei113 – 1131PhosphoserineBy similarity
Modified residuei119 – 1191PhosphoserineBy similarity
Modified residuei120 – 1201PhosphoserineBy similarity
Modified residuei244 – 2441PhosphothreonineBy similarity
Modified residuei515 – 5151PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ99MJ9.
MaxQBiQ99MJ9.
PaxDbiQ99MJ9.
PRIDEiQ99MJ9.

PTM databases

iPTMnetiQ99MJ9.
PhosphoSiteiQ99MJ9.

Expressioni

Gene expression databases

BgeeiQ99MJ9.
CleanExiMM_DDX50.
GenevisibleiQ99MJ9. MM.

Interactioni

Subunit structurei

Interacts with C1QBP.By similarity

Protein-protein interaction databases

BioGridi220470. 1 interaction.
IntActiQ99MJ9. 1 interaction.
MINTiMINT-4092991.
STRINGi10090.ENSMUSP00000020270.

Structurei

3D structure databases

ProteinModelPortaliQ99MJ9.
SMRiQ99MJ9. Positions 118-656.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini165 – 344180Helicase ATP-bindingPROSITE-ProRule annotationAdd
BLAST
Domaini377 – 521145Helicase C-terminalPROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi134 – 16229Q motifAdd
BLAST
Motifi287 – 2904DEVD box

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi674 – 72552Arg-richAdd
BLAST

Sequence similaritiesi

Contains 1 helicase ATP-binding domain.PROSITE-ProRule annotation
Contains 1 helicase C-terminal domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0331. Eukaryota.
COG0513. LUCA.
GeneTreeiENSGT00840000129877.
HOGENOMiHOG000268805.
HOVERGENiHBG051331.
InParanoidiQ99MJ9.
KOiK13183.
OMAiCEKKSKR.
OrthoDBiEOG77HDD6.
PhylomeDBiQ99MJ9.
TreeFamiTF328622.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR011545. DEAD/DEAH_box_helicase_dom.
IPR012562. GUCT.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR012677. Nucleotide-bd_a/b_plait.
IPR027417. P-loop_NTPase.
IPR014014. RNA_helicase_DEAD_Q_motif.
[Graphical view]
PfamiPF00270. DEAD. 1 hit.
PF08152. GUCT. 1 hit.
PF00271. Helicase_C. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
SSF54928. SSF54928. 1 hit.
PROSITEiPS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS51195. Q_MOTIF. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q99MJ9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPGKLLWGDI MELEAPLEES ESQRKERQKS DRRKSRHHSE SEERTETREN
60 70 80 90 100
GVTDDLDAPK PKKAKMREKL NGDTKEGLRF SDEFSPSHKS RRKDLPNGDV
110 120 130 140 150
DEYEKRSKRV SSSENSHKSS DKAEETLTRE QKEGAFSNFS ISEETIKLLK
160 170 180 190 200
GRGVTYLFPI QVKTFGPVYE GKDLIAQART GTGKTFSFAI PLIERLQRNQ
210 220 230 240 250
ETIKKSRSPK VLVLAPTREL ANQVAKDFKD ITRKLSVACF YGGTSYQSQI
260 270 280 290 300
NQIRNGIDIL VGTPGRIKDH LQSGRLDLSK LRHVVLDEVD QMLDLGFAEQ
310 320 330 340 350
VEDIIHESYK TDSEDNPQTL LFSATCPQWV YKVAKKYMKS RYEQVDLVGK
360 370 380 390 400
MTQKAATTVE HLAIQCHWSQ RPAVIGDVLQ VYSGSEGRAI IFCETKKNVT
410 420 430 440 450
EMAMNPHIKQ NAQCLHGDIA QSQREITLKG FREGSFKVLV ATNVAARGLD
460 470 480 490 500
IPEVDLVIQS SPPQDVESYI HRSGRTGRAG RTGICVCFYQ PRERGQLRYV
510 520 530 540 550
EQKAGITFKR VGVPSTMDLV KSKSMDAIRS LASVSYAAVD FFRPSAQRLI
560 570 580 590 600
EEKGAVDALA AALAHISGAS SFEPRSLITS DKGFVTMTLE SPEEIQDVSC
610 620 630 640 650
AWKELNRKLS SNAVSHVTRM CLLKGNMGVC FDVPTSESER LQAEWHDSDW
660 670 680 690 700
ILSVPAKLPE IEEYYDGNTS SNPRQRSGWS GGRSGRSGRS GGRSGGRSGR
710 720 730
QSRQGSRSGS RQDGRRRSGN RNRSRSGGHK RNFD
Length:734
Mass (Da):82,175
Last modified:June 1, 2001 - v1
Checksum:iE816B0B2555EE20A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF334104 mRNA. Translation: AAK29403.1.
CCDSiCCDS23892.1.
RefSeqiNP_444413.1. NM_053183.2.
UniGeneiMm.114116.

Genome annotation databases

EnsembliENSMUST00000020270; ENSMUSP00000020270; ENSMUSG00000020076.
GeneIDi94213.
KEGGimmu:94213.
UCSCiuc007fho.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF334104 mRNA. Translation: AAK29403.1.
CCDSiCCDS23892.1.
RefSeqiNP_444413.1. NM_053183.2.
UniGeneiMm.114116.

3D structure databases

ProteinModelPortaliQ99MJ9.
SMRiQ99MJ9. Positions 118-656.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi220470. 1 interaction.
IntActiQ99MJ9. 1 interaction.
MINTiMINT-4092991.
STRINGi10090.ENSMUSP00000020270.

PTM databases

iPTMnetiQ99MJ9.
PhosphoSiteiQ99MJ9.

Proteomic databases

EPDiQ99MJ9.
MaxQBiQ99MJ9.
PaxDbiQ99MJ9.
PRIDEiQ99MJ9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000020270; ENSMUSP00000020270; ENSMUSG00000020076.
GeneIDi94213.
KEGGimmu:94213.
UCSCiuc007fho.1. mouse.

Organism-specific databases

CTDi79009.
MGIiMGI:2182303. Ddx50.

Phylogenomic databases

eggNOGiKOG0331. Eukaryota.
COG0513. LUCA.
GeneTreeiENSGT00840000129877.
HOGENOMiHOG000268805.
HOVERGENiHBG051331.
InParanoidiQ99MJ9.
KOiK13183.
OMAiCEKKSKR.
OrthoDBiEOG77HDD6.
PhylomeDBiQ99MJ9.
TreeFamiTF328622.

Miscellaneous databases

PROiQ99MJ9.
SOURCEiSearch...

Gene expression databases

BgeeiQ99MJ9.
CleanExiMM_DDX50.
GenevisibleiQ99MJ9. MM.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR011545. DEAD/DEAH_box_helicase_dom.
IPR012562. GUCT.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR012677. Nucleotide-bd_a/b_plait.
IPR027417. P-loop_NTPase.
IPR014014. RNA_helicase_DEAD_Q_motif.
[Graphical view]
PfamiPF00270. DEAD. 1 hit.
PF08152. GUCT. 1 hit.
PF00271. Helicase_C. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
SSF54928. SSF54928. 1 hit.
PROSITEiPS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS51195. Q_MOTIF. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Genomic structure of newly identified paralogue of RNA helicase II/Gu: detection of pseudogenes and multiple alternatively spliced mRNAs."
    Valdez B.C., Yang H., Hong E., Sequitin A.M.
    Gene 284:53-61(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Entry informationi

Entry nameiDDX50_MOUSE
AccessioniPrimary (citable) accession number: Q99MJ9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 23, 2004
Last sequence update: June 1, 2001
Last modified: June 8, 2016
This is version 113 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.