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Protein

Na(+)/H(+) exchange regulatory cofactor NHE-RF4

Gene

Pdzd3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Acts as a regulatory protein that associates with GUCY2C and negatively modulates its heat-stable enterotoxin-mediated activation. Stimulates SLC9A3 activity in the presence of elevated calcium ions (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
Na(+)/H(+) exchange regulatory cofactor NHE-RF4
Short name:
NHERF-4
Alternative name(s):
Natrium-phosphate cotransporter IIa C-terminal-associated protein 2
Short name:
Na/Pi cotransporter C-terminal-associated protein 2
Short name:
NaPi-Cap2
PDZ domain-containing protein 2
PDZ domain-containing protein 3
Sodium-hydrogen exchanger regulatory factor 4
Gene namesi
Name:Pdzd3
Synonyms:Nherf4, Pdzk2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:2429554. Pdzd3.

Subcellular locationi

GO - Cellular componenti

  • apical part of cell Source: UniProtKB
  • brush border Source: UniProtKB
  • cytoplasm Source: UniProtKB-SubCell
  • subapical complex Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 498498Na(+)/H(+) exchange regulatory cofactor NHE-RF4PRO_0000058293Add
BLAST

Proteomic databases

MaxQBiQ99MJ6.
PaxDbiQ99MJ6.
PeptideAtlasiQ99MJ6.
PRIDEiQ99MJ6.

PTM databases

iPTMnetiQ99MJ6.
PhosphoSiteiQ99MJ6.

Expressioni

Tissue specificityi

Expressed in kidney and small intestine. Not detected in heart, brain, spleen, lung, liver, skeletal muscle or testis.1 Publication

Gene expression databases

BgeeiQ99MJ6.
CleanExiMM_PDZD3.

Interactioni

Subunit structurei

Interacts with the C-terminal region of GUCY2C. Interacts with C-terminal region of SLC9A3 and the interactions decrease in response to elevated calcium ion levels (By similarity). Interacts with the C-terminal region of SLC34A1.By similarity1 Publication

GO - Molecular functioni

Protein-protein interaction databases

BioGridi228423. 1 interaction.
IntActiQ99MJ6. 2 interactions.
MINTiMINT-152467.
STRINGi10090.ENSMUSP00000034618.

Structurei

3D structure databases

ProteinModelPortaliQ99MJ6.
SMRiQ99MJ6. Positions 47-127, 150-349, 393-474.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini49 – 13082PDZ 1PROSITE-ProRule annotationAdd
BLAST
Domaini157 – 23579PDZ 2PROSITE-ProRule annotationAdd
BLAST
Domaini263 – 34684PDZ 3PROSITE-ProRule annotationAdd
BLAST
Domaini394 – 47582PDZ 4PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 4 PDZ (DHR) domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiENOG410ITA7. Eukaryota.
ENOG410XURS. LUCA.
HOGENOMiHOG000048712.
HOVERGENiHBG080760.
InParanoidiQ99MJ6.
OrthoDBiEOG73V6K7.
PhylomeDBiQ99MJ6.
TreeFamiTF350449.

Family and domain databases

Gene3Di2.30.42.10. 4 hits.
InterProiIPR031200. NHERF-4.
IPR001478. PDZ.
[Graphical view]
PANTHERiPTHR14191:SF5. PTHR14191:SF5. 1 hit.
PfamiPF00595. PDZ. 4 hits.
[Graphical view]
SMARTiSM00228. PDZ. 4 hits.
[Graphical view]
SUPFAMiSSF50156. SSF50156. 4 hits.
PROSITEiPS50106. PDZ. 4 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q99MJ6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEAAADLRDT ALLTLKFKFN PRLGIDNPVL SLAEDQDQSD PWNLHRPRFC
60 70 80 90 100
LLSKEEEKTF GFHLQQHLGK ADHVVCRVDP GTSAQRQGLR EGDRILAVNN
110 120 130 140 150
NIVAHEDHAV VVRYIRASGP RVLLTVLAQH VHDVARVLQG SDAFLCPTLP
160 170 180 190 200
SGVRPRLCHV VKDEGGFGFS VTHGSRGPFW LVLSAGGAAE RAGVPPGARL
210 220 230 240 250
LEVNGASVEK LTYNQLNRKL WQSGDQVTLL VAGLEVEEQC HQLGMPLAAP
260 270 280 290 300
LAEGWALPAK PRCLNIEKGP EGFGFLLREE KGLDGRLGQF LWDVDPGLPA
310 320 330 340 350
DKAGMKAGDR LVAVAGESVD GLGHEETVSR IRAQGSCVSL IVVDPEADRF
360 370 380 390 400
FSMVRLSPLL FLENTEIAAP PLAETKDLPV EDTVEPSGLA GSCQCFLYPG
410 420 430 440 450
PGGGYGFRLC CVASGPCLFI SQVTPGGSTA RAGLQVGDTV LEVNGYPVGG
460 470 480 490
DSELDRLQQL TEAEPPLCLK LGARNPQGLE AWISLESGED WTLASELL
Length:498
Mass (Da):53,525
Last modified:June 1, 2001 - v1
Checksum:iCD979649B08E475A
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti429 – 4291T → A in BAC35130 (PubMed:16141072).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF334612 mRNA. Translation: AAK20865.1.
AK052748 mRNA. Translation: BAC35130.1.
CCDSiCCDS23099.1.
RefSeqiNP_573489.2. NM_133226.2.
UniGeneiMm.29872.

Genome annotation databases

GeneIDi170761.
KEGGimmu:170761.
UCSCiuc009pch.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF334612 mRNA. Translation: AAK20865.1.
AK052748 mRNA. Translation: BAC35130.1.
CCDSiCCDS23099.1.
RefSeqiNP_573489.2. NM_133226.2.
UniGeneiMm.29872.

3D structure databases

ProteinModelPortaliQ99MJ6.
SMRiQ99MJ6. Positions 47-127, 150-349, 393-474.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi228423. 1 interaction.
IntActiQ99MJ6. 2 interactions.
MINTiMINT-152467.
STRINGi10090.ENSMUSP00000034618.

PTM databases

iPTMnetiQ99MJ6.
PhosphoSiteiQ99MJ6.

Proteomic databases

MaxQBiQ99MJ6.
PaxDbiQ99MJ6.
PeptideAtlasiQ99MJ6.
PRIDEiQ99MJ6.

Protocols and materials databases

DNASUi170761.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi170761.
KEGGimmu:170761.
UCSCiuc009pch.1. mouse.

Organism-specific databases

CTDi79849.
MGIiMGI:2429554. Pdzd3.

Phylogenomic databases

eggNOGiENOG410ITA7. Eukaryota.
ENOG410XURS. LUCA.
HOGENOMiHOG000048712.
HOVERGENiHBG080760.
InParanoidiQ99MJ6.
OrthoDBiEOG73V6K7.
PhylomeDBiQ99MJ6.
TreeFamiTF350449.

Miscellaneous databases

PROiQ99MJ6.
SOURCEiSearch...

Gene expression databases

BgeeiQ99MJ6.
CleanExiMM_PDZD3.

Family and domain databases

Gene3Di2.30.42.10. 4 hits.
InterProiIPR031200. NHERF-4.
IPR001478. PDZ.
[Graphical view]
PANTHERiPTHR14191:SF5. PTHR14191:SF5. 1 hit.
PfamiPF00595. PDZ. 4 hits.
[Graphical view]
SMARTiSM00228. PDZ. 4 hits.
[Graphical view]
SUPFAMiSSF50156. SSF50156. 4 hits.
PROSITEiPS50106. PDZ. 4 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Interaction of the type IIa Na/Pi-cotransporter with PDZ proteins."
    Gisler S.M., Stagljar I., Traebert M., Bacic D., Biber J., Murer H.
    J. Biol. Chem. 276:9206-9213(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], INTERACTION WITH SLC34A1, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
    Strain: BALB/cJImported.
    Tissue: KidneyImported.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Kidney.
  3. "Elevated intracellular calcium stimulates NHE3 activity by an IKEPP (NHERF4) dependent mechanism."
    Zachos N.C., Hodson C., Kovbasnjuk O., Li X., Thelin W.R., Cha B., Milgram S., Donowitz M.
    Cell. Physiol. Biochem. 22:693-704(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.

Entry informationi

Entry nameiNHRF4_MOUSE
AccessioniPrimary (citable) accession number: Q99MJ6
Secondary accession number(s): Q8BWE5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 16, 2005
Last sequence update: June 1, 2001
Last modified: July 6, 2016
This is version 112 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.