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Q99MJ6 (NHRF4_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 83. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Na(+)/H(+) exchange regulatory cofactor NHE-RF4
Short name=NHERF-4
Alternative name(s):
Natrium-phosphate cotransporter IIa C-terminal-associated protein 2
Short name=Na/Pi cotransporter C-terminal-associated protein 2
Short name=NaPi-Cap2
PDZ domain-containing protein 2
PDZ domain-containing protein 3
Sodium-hydrogen exchanger regulatory factor 4
Gene names
Name:Pdzd3
Synonyms:Nherf4, Pdzk2
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length498 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Acts as a regulatory protein that associates with GUCY2C and negatively modulates its heat-stable enterotoxin-mediated activation. Stimulates SLC9A3 activity in the presence of elevated calcium ions By similarity. UniProtKB Q86UT5

Subunit structure

Interacts with the C-terminal region of GUCY2C. Interacts with C-terminal region of SLC9A3 and the interactions decrease in response to elevated calcium ion levels By similarity. Interacts with the C-terminal region of SLC34A1. Ref.1

Subcellular location

Membrane; Peripheral membrane protein By similarity. Cytoplasm By similarity. Endosome membrane; Peripheral membrane protein By similarity. Note: In kidney, predominantly localized to the subapical compartment of proximal tubular cells. In intestinal epithelial cells, localizes to the ileal brush border and in a juxtanuclear compartment. Ref.1 Ref.3

Tissue specificity

Expressed in kidney and small intestine. Not detected in heart, brain, spleen, lung, liver, skeletal muscle or testis. Ref.1

Sequence similarities

Contains 4 PDZ (DHR) domains.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 498498Na(+)/H(+) exchange regulatory cofactor NHE-RF4
PRO_0000058293

Regions

Domain49 – 13082PDZ 1
Domain157 – 23579PDZ 2
Domain263 – 34684PDZ 3
Domain394 – 47582PDZ 4

Experimental info

Sequence conflict4291T → A in BAC35130. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Q99MJ6 [UniParc].

Last modified June 1, 2001. Version 1.
Checksum: CD979649B08E475A

FASTA49853,525
        10         20         30         40         50         60 
MEAAADLRDT ALLTLKFKFN PRLGIDNPVL SLAEDQDQSD PWNLHRPRFC LLSKEEEKTF 

        70         80         90        100        110        120 
GFHLQQHLGK ADHVVCRVDP GTSAQRQGLR EGDRILAVNN NIVAHEDHAV VVRYIRASGP 

       130        140        150        160        170        180 
RVLLTVLAQH VHDVARVLQG SDAFLCPTLP SGVRPRLCHV VKDEGGFGFS VTHGSRGPFW 

       190        200        210        220        230        240 
LVLSAGGAAE RAGVPPGARL LEVNGASVEK LTYNQLNRKL WQSGDQVTLL VAGLEVEEQC 

       250        260        270        280        290        300 
HQLGMPLAAP LAEGWALPAK PRCLNIEKGP EGFGFLLREE KGLDGRLGQF LWDVDPGLPA 

       310        320        330        340        350        360 
DKAGMKAGDR LVAVAGESVD GLGHEETVSR IRAQGSCVSL IVVDPEADRF FSMVRLSPLL 

       370        380        390        400        410        420 
FLENTEIAAP PLAETKDLPV EDTVEPSGLA GSCQCFLYPG PGGGYGFRLC CVASGPCLFI 

       430        440        450        460        470        480 
SQVTPGGSTA RAGLQVGDTV LEVNGYPVGG DSELDRLQQL TEAEPPLCLK LGARNPQGLE 

       490 
AWISLESGED WTLASELL

« Hide

References

« Hide 'large scale' references
[1]"Interaction of the type IIa Na/Pi-cotransporter with PDZ proteins."
Gisler S.M., Stagljar I., Traebert M., Bacic D., Biber J., Murer H.
J. Biol. Chem. 276:9206-9213(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], INTERACTION WITH SLC34A1, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
Strain: BALB/c.
Tissue: Kidney.
[2]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6J.
Tissue: Kidney.
[3]"Elevated intracellular calcium stimulates NHE3 activity by an IKEPP (NHERF4) dependent mechanism."
Zachos N.C., Hodson C., Kovbasnjuk O., Li X., Thelin W.R., Cha B., Milgram S., Donowitz M.
Cell. Physiol. Biochem. 22:693-704(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AF334612 mRNA. Translation: AAK20865.1.
AK052748 mRNA. Translation: BAC35130.1.
IPIIPI00117768.
RefSeqNP_573489.2. NM_133226.2.
UniGeneMm.29872.

3D structure databases

ProteinModelPortalQ99MJ6.
SMRQ99MJ6. Positions 47-127, 155-235, 260-349, 392-472.
ModBaseSearch...

Protein-protein interaction databases

MINTMINT-152467.

PTM databases

PhosphoSiteQ99MJ6.

Proteomic databases

PaxDbQ99MJ6.
PRIDEQ99MJ6.

Protocols and materials databases

DNASU170761.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000034618; ENSMUSP00000034618; ENSMUSG00000032105.
GeneID170761.
KEGGmmu:170761.
UCSCuc009pch.1. mouse.

Organism-specific databases

CTD79849.
MGIMGI:2429554. Pdzd3.

Phylogenomic databases

eggNOGNOG262468.
GeneTreeENSGT00530000062999.
HOGENOMHOG000048712.
HOVERGENHBG080760.
InParanoidQ99MJ6.
OrthoDBEOG412M5D.

Gene expression databases

BgeeQ99MJ6.
CleanExMM_PDZD3.
GenevestigatorQ99MJ6.
GermOnlineENSMUSG00000032105. Mus musculus.

Family and domain databases

InterProIPR001478. PDZ.
[Graphical view]
PfamPF00595. PDZ. 4 hits.
[Graphical view]
SMARTSM00228. PDZ. 4 hits.
[Graphical view]
SUPFAMSSF50156. PDZ. 4 hits.
PROSITEPS50106. PDZ. 4 hits.
[Graphical view]
ProtoNetSearch...

Other

NextBio370366.
SOURCESearch...

Entry information

Entry nameNHRF4_MOUSE
AccessionPrimary (citable) accession number: Q99MJ6
Secondary accession number(s): Q8BWE5
Entry history
Integrated into UniProtKB/Swiss-Prot: August 16, 2005
Last sequence update: June 1, 2001
Last modified: May 1, 2013
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

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