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Protein

ELKS/Rab6-interacting/CAST family member 1

Gene

Erc1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Regulatory subunit of the IKK complex. Probably recruits IkappaBalpha/NFKBIA to the complex (By similarity). May be involved in the organization of the cytomatrix at the nerve terminals active zone (CAZ) which regulates neurotransmitter release. May be involved in vesicle trafficking at the CAZ. May be involved in Rab-6 regulated endosomes to Golgi transport.By similarity

GO - Molecular functioni

  • PDZ domain binding Source: ParkinsonsUK-UCL
  • Rab GTPase binding Source: MGI

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
ELKS/Rab6-interacting/CAST family member 1
Short name:
ERC-1
Alternative name(s):
CAZ-associated structural protein 2
Short name:
CAST2
Rab6-interacting protein 2
Gene namesi
Name:Erc1
Synonyms:Cast2, Kiaa1081, Rab6ip2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 6

Organism-specific databases

MGIiMGI:2151013. Erc1.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: MGI
  • Golgi membrane Source: UniProtKB-SubCell
  • IkappaB kinase complex Source: MGI
  • presynaptic active zone Source: InterPro
  • synapse Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 11201120ELKS/Rab6-interacting/CAST family member 1PRO_0000097177Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei10 – 101N6-acetyllysineCombined sources
Modified residuei17 – 171PhosphoserineBy similarity
Modified residuei21 – 211PhosphoserineCombined sources
Modified residuei37 – 371PhosphoserineBy similarity
Modified residuei38 – 381PhosphothreonineBy similarity
Modified residuei55 – 551PhosphoserineCombined sources
Modified residuei75 – 751PhosphoserineCombined sources
Modified residuei94 – 941PhosphoserineBy similarity
Modified residuei824 – 8241PhosphoserineCombined sources
Modified residuei1009 – 10091PhosphoserineBy similarity
Modified residuei1050 – 10501PhosphothreonineBy similarity
Isoform 2 (identifier: Q99MI1-2)
Modified residuei965 – 9651PhosphoserineCombined sources

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ99MI1.
MaxQBiQ99MI1.
PaxDbiQ99MI1.
PeptideAtlasiQ99MI1.
PRIDEiQ99MI1.

PTM databases

iPTMnetiQ99MI1.
PhosphoSiteiQ99MI1.

Expressioni

Tissue specificityi

Widely expressed.1 Publication

Gene expression databases

BgeeiENSMUSG00000030172.
CleanExiMM_ERC1.
ExpressionAtlasiQ99MI1. baseline and differential.
GenevisibleiQ99MI1. MM.

Interactioni

Subunit structurei

Interacts with the GTB-bound forms of RAB6A isoform 1 and isoform 2 and with RAB6B. The interaction was strongest with RAB6B, followed by RAB6A isoform 2 and weakest with RAB6A isoform 1. Part of a complex with CHUK, IKBKB and IKBKG. Interacts with CHUK, IKBKB and IKBKG. The interaction with IKBKG is independent of CHUK and IKBKB. Interacts with NFKBIA (By similarity). Isoform 2 interacts through its C-terminus with the PDZ domains of RIMS1 and RIMS2. Interacts with ERC2/CAST1 (By similarity).By similarity

GO - Molecular functioni

  • PDZ domain binding Source: ParkinsonsUK-UCL
  • Rab GTPase binding Source: MGI

Protein-protein interaction databases

BioGridi226265. 2 interactions.
IntActiQ99MI1. 5 interactions.
MINTiMINT-4094612.
STRINGi10090.ENSMUSP00000032279.

Structurei

3D structure databases

ProteinModelPortaliQ99MI1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1050 – 111263FIP-RBDPROSITE-ProRule annotationAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili144 – 992849Sequence analysisAdd
BLAST
Coiled coili1060 – 110445Sequence analysisAdd
BLAST

Sequence similaritiesi

Contains 1 FIP-RBD domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG4809. Eukaryota.
ENOG410Y4GU. LUCA.
GeneTreeiENSGT00650000093320.
HOGENOMiHOG000236353.
HOVERGENiHBG051496.
InParanoidiQ99MI1.
KOiK16072.
PhylomeDBiQ99MI1.

Family and domain databases

InterProiIPR019323. ELKS/CAST.
IPR019018. Rab-bd_FIP-RBD.
[Graphical view]
PANTHERiPTHR18861. PTHR18861. 2 hits.
PfamiPF10174. Cast. 3 hits.
PF09457. RBD-FIP. 1 hit.
[Graphical view]
PROSITEiPS51511. FIP_RBD. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q99MI1-1) [UniParc]FASTAAdd to basket
Also known as: B, beta

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MYGSARSVGK VEPSSQSPGR SPRLPRSPRL GHRRTNSTGG SSGNSVGGGS
60 70 80 90 100
GKTLSMENIQ SLNAAYATSG PMYLSDHENV GAETPKSTMT LGRSGGRLPY
110 120 130 140 150
GVRMTAMGSS PNIASSGVAS DTIAFGEHHL PPVSMASTVP HSLRQARDNT
160 170 180 190 200
IMDLQTQLKE VLRENDLLRK DVEVKESKLS SSMNSIKTFW SPELKKERAL
210 220 230 240 250
RKDEASKITI WKEQYRVVQE ENQHMQMTIQ ALQDELRIQR DLNQLFQQDS
260 270 280 290 300
SSRTGEPCVA ELTEENFQRL HAEHERQAKE LFLLRKTLEE MELRIETQKQ
310 320 330 340 350
TLNARDESIK KLLEMLQSKG LSAKATEEDH ERTRRLAEAE MHVHHLESLL
360 370 380 390 400
EQKEKENNML REEMHRRFEN APDSAKTKAL QTVIEMKDSK ISSMERGLRD
410 420 430 440 450
LEEEIQMLKS NGALSSEERE EEMKQMEVYR SHSKFMKNKV EQLKEELSSK
460 470 480 490 500
DAQGEELKKR AAGLQSEIGQ VKQELSRKDT ELLALQTKLE TLTNQFSDSK
510 520 530 540 550
QHIEVLKESL TAKEQRAAIL QTEVDALRLR LEEKETMLNK KTKQIQDMAE
560 570 580 590 600
EKGTQAGEIH DLKDMLDVKE RKVNVLQKKI ENLQEQLRDK EKQMSSLKER
610 620 630 640 650
VKSLQADTTN TDTALTTLEE ALADKERTIE RLKEQRDRDE REKQEEIDTY
660 670 680 690 700
KKDLKDLREK VSLLQGDLSE KEASLLDIKE HASSLASSGL KKDSRLKTLE
710 720 730 740 750
IALEQKKEEC LKMESQLKKA HEATLEARAS PEMSDRIQQL EREISRYKDE
760 770 780 790 800
SSKAQTEVDR LLEILKEVEN EKNDKDKKIA ELESLTSRQV KDQNKKVANL
810 820 830 840 850
KHKEQVEKKK SAQMLEEARR REDSLSDSSQ QLQDSLRKKD DRIEELEEAL
860 870 880 890 900
RESVQITAER EMVLAQEESA RTNAEKQVEE LLMAMEKVKQ ELESMKAKLS
910 920 930 940 950
STQQSLAEKE THLTNLRAER RKHLEEVLEM KQEALLAAIS EKDANIALLE
960 970 980 990 1000
LSSSKKKTQE EVAALKREKD RLVQQLKQQT QNRMKLMADN YEDDHFRSSR
1010 1020 1030 1040 1050
SNQTNHKPSP DQIIQPLLEL DQNRSKLKLY IGHLTALCHD RDPLILRGLT
1060 1070 1080 1090 1100
PPASYNADGE QAAWENELQQ MTQEQLQNEL EKVEGDNAEL QEFANTILQQ
1110 1120
IADHCPDILE QVVNALEESS
Length:1,120
Mass (Da):128,331
Last modified:June 1, 2001 - v1
Checksum:iA542B526FAEDF9C7
GO
Isoform 2 (identifier: Q99MI1-2) [UniParc]FASTAAdd to basket
Also known as: A

The sequence of this isoform differs from the canonical sequence as follows:
     834-877: Missing.
     1013-1020: IIQPLLEL → DEEEGIWA
     1021-1120: Missing.

Note: May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay.Combined sources
Show »
Length:976
Mass (Da):111,931
Checksum:i48731867C8D8CD6F
GO
Isoform 3 (identifier: Q99MI1-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-151: Missing.
     440-467: Missing.
     525-547: DALRLRLEEKETMLNKKTKQIQD → KSFCDLCRIQSIPSFILLYICYV
     548-1120: Missing.

Show »
Length:368
Mass (Da):43,425
Checksum:iA404016D6BD06F06
GO

Sequence cautioni

The sequence BAC65719 differs from that shown.Artifact. Missing internal sequence that does not correspond to an exon-intron boundary.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti121 – 1211D → G in BAC35542 (PubMed:16141072).Curated
Sequence conflicti1070 – 10701Q → K in BAC65719 (PubMed:12693553).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 151151Missing in isoform 3. 1 PublicationVSP_011456Add
BLAST
Alternative sequencei440 – 46728Missing in isoform 3. 1 PublicationVSP_011457Add
BLAST
Alternative sequencei525 – 54723DALRL…KQIQD → KSFCDLCRIQSIPSFILLYI CYV in isoform 3. 1 PublicationVSP_011458Add
BLAST
Alternative sequencei548 – 1120573Missing in isoform 3. 1 PublicationVSP_011459Add
BLAST
Alternative sequencei834 – 87744Missing in isoform 2. 2 PublicationsVSP_011460Add
BLAST
Alternative sequencei1013 – 10208IIQPLLEL → DEEEGIWA in isoform 2. 2 PublicationsVSP_011462
Alternative sequencei1021 – 1120100Missing in isoform 2. 2 PublicationsVSP_011463Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF340028 mRNA. Translation: AAK26381.1.
AF340029 mRNA. Translation: AAK26382.1.
AY316692 mRNA. Translation: AAP83581.1.
AK048990 mRNA. Translation: BAC33505.1.
AK053824 mRNA. Translation: BAC35542.1.
AK122437 Transcribed RNA. Translation: BAC65719.1. Sequence problems.
CCDSiCCDS20476.1. [Q99MI1-4]
CCDS39611.1. [Q99MI1-1]
RefSeqiNP_835186.1. NM_178085.3. [Q99MI1-4]
UniGeneiMm.288860.
Mm.446801.

Genome annotation databases

EnsembliENSMUST00000079582; ENSMUSP00000078534; ENSMUSG00000030172. [Q99MI1-4]
ENSMUST00000184838; ENSMUSP00000139030; ENSMUSG00000030172. [Q99MI1-2]
GeneIDi111173.
KEGGimmu:111173.
UCSCiuc009dmn.1. mouse. [Q99MI1-4]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF340028 mRNA. Translation: AAK26381.1.
AF340029 mRNA. Translation: AAK26382.1.
AY316692 mRNA. Translation: AAP83581.1.
AK048990 mRNA. Translation: BAC33505.1.
AK053824 mRNA. Translation: BAC35542.1.
AK122437 Transcribed RNA. Translation: BAC65719.1. Sequence problems.
CCDSiCCDS20476.1. [Q99MI1-4]
CCDS39611.1. [Q99MI1-1]
RefSeqiNP_835186.1. NM_178085.3. [Q99MI1-4]
UniGeneiMm.288860.
Mm.446801.

3D structure databases

ProteinModelPortaliQ99MI1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi226265. 2 interactions.
IntActiQ99MI1. 5 interactions.
MINTiMINT-4094612.
STRINGi10090.ENSMUSP00000032279.

PTM databases

iPTMnetiQ99MI1.
PhosphoSiteiQ99MI1.

Proteomic databases

EPDiQ99MI1.
MaxQBiQ99MI1.
PaxDbiQ99MI1.
PeptideAtlasiQ99MI1.
PRIDEiQ99MI1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000079582; ENSMUSP00000078534; ENSMUSG00000030172. [Q99MI1-4]
ENSMUST00000184838; ENSMUSP00000139030; ENSMUSG00000030172. [Q99MI1-2]
GeneIDi111173.
KEGGimmu:111173.
UCSCiuc009dmn.1. mouse. [Q99MI1-4]

Organism-specific databases

CTDi23085.
MGIiMGI:2151013. Erc1.
RougeiSearch...

Phylogenomic databases

eggNOGiKOG4809. Eukaryota.
ENOG410Y4GU. LUCA.
GeneTreeiENSGT00650000093320.
HOGENOMiHOG000236353.
HOVERGENiHBG051496.
InParanoidiQ99MI1.
KOiK16072.
PhylomeDBiQ99MI1.

Miscellaneous databases

ChiTaRSiErc1. mouse.
PROiQ99MI1.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000030172.
CleanExiMM_ERC1.
ExpressionAtlasiQ99MI1. baseline and differential.
GenevisibleiQ99MI1. MM.

Family and domain databases

InterProiIPR019323. ELKS/CAST.
IPR019018. Rab-bd_FIP-RBD.
[Graphical view]
PANTHERiPTHR18861. PTHR18861. 2 hits.
PfamiPF10174. Cast. 3 hits.
PF09457. RBD-FIP. 1 hit.
[Graphical view]
PROSITEiPS51511. FIP_RBD. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRB6I2_MOUSE
AccessioniPrimary (citable) accession number: Q99MI1
Secondary accession number(s): Q80TK7
, Q8BPL1, Q8C7Y1, Q99MI2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 31, 2004
Last sequence update: June 1, 2001
Last modified: September 7, 2016
This is version 122 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.