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Protein

Nucleoside diphosphate kinase homolog 5

Gene

Nme5

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Does not seem to have NDK kinase activity. Confers protection from cell death by Bax and alters the cellular levels of several antioxidant enzymes including Gpx5. May play a role in spermiogenesis by increasing the ability of late-stage spermatids to eliminate reactive oxygen species.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei127 – 1271Pros-phosphohistidine intermediateSequence analysis

GO - Molecular functioni

GO - Biological processi

  • cilium assembly Source: MGI
  • CTP biosynthetic process Source: InterPro
  • epithelial cilium movement Source: MGI
  • GTP biosynthetic process Source: InterPro
  • negative regulation of oxidative stress-induced intrinsic apoptotic signaling pathway Source: MGI
  • response to reactive oxygen species Source: UniProtKB
  • spermatid development Source: UniProtKB
  • UTP biosynthetic process Source: InterPro
  • ventricular system development Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Biological processi

Differentiation, Spermatogenesis

Names & Taxonomyi

Protein namesi
Recommended name:
Nucleoside diphosphate kinase homolog 5
Short name:
NDK-H 5
Short name:
NDP kinase homolog 5
Alternative name(s):
nm23-M5
Gene namesi
Name:Nme5
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 18

Organism-specific databases

MGIiMGI:1922783. Nme5.

Subcellular locationi

GO - Cellular componenti

  • intracellular Source: GO_Central
  • sperm flagellum Source: MGI
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 211211Nucleoside diphosphate kinase homolog 5PRO_0000137126Add
BLAST

Proteomic databases

MaxQBiQ99MH5.
PaxDbiQ99MH5.
PRIDEiQ99MH5.

PTM databases

PhosphoSiteiQ99MH5.

Expressioni

Tissue specificityi

Expressed predominantly in germ cells of the testis. Not expressed in testicular somatic cells.1 Publication

Developmental stagei

First appears in pachytene spermatocytes and increases in abundance in subsequent stages.1 Publication

Gene expression databases

BgeeiQ99MH5.
ExpressionAtlasiQ99MH5. baseline and differential.
GenevisibleiQ99MH5. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000078269.

Structurei

3D structure databases

ProteinModelPortaliQ99MH5.
SMRiQ99MH5. Positions 13-143, 156-198.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the NDK family.Curated

Phylogenomic databases

eggNOGiKOG0888. Eukaryota.
COG0105. LUCA.
GeneTreeiENSGT00760000119146.
HOGENOMiHOG000224565.
HOVERGENiHBG000198.
InParanoidiQ99MH5.
KOiK00940.
OMAiDFYAEHY.
OrthoDBiEOG7NSB3J.
PhylomeDBiQ99MH5.
TreeFamiTF354225.

Family and domain databases

InterProiIPR007858. Dpy-30_motif.
IPR012410. NDK_H5.
IPR001564. Nucleoside_diP_kinase.
[Graphical view]
PfamiPF05186. Dpy-30. 1 hit.
PF00334. NDK. 1 hit.
[Graphical view]
PIRSFiPIRSF036504. NDK_H5. 1 hit.
PRINTSiPR01243. NUCDPKINASE.
SMARTiSM00562. NDK. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Note: Additional isoforms seem to exist.

Isoform 1 (identifier: Q99MH5-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEVSMPLPQI YVEKTLALIK PDVVDKEEEI QDIILGSGFT IIQRRKLHLS
60 70 80 90 100
PEHCSNFYVE QYGKMFFPNL TAYMSSGPLV AMILARHKAI SYWKELMGPS
110 120 130 140 150
NSLVAKETHP DSLRAIYGTD ELRNALHGSN DFAASEREIR FMFPAVIIEP
160 170 180 190 200
IPIGQAAKDY INLYVAPTLL QGLTELCKEK PPDPYLWLAD WLMKNNPNKP
210
KLCHFPVTEE P
Length:211
Mass (Da):24,002
Last modified:July 11, 2003 - v2
Checksum:i3624D0C76BDF3ADD
GO
Isoform 2 (identifier: Q99MH5-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     113-114: LR → DY
     115-211: Missing.

Show »
Length:114
Mass (Da):13,018
Checksum:i29DBC942282C3F02
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti2 – 21E → V in AAK20866 (PubMed:12788088).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei113 – 1142LR → DY in isoform 2. 2 PublicationsVSP_007773
Alternative sequencei115 – 21197Missing in isoform 2. 2 PublicationsVSP_007774Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF343565 mRNA. Translation: AAK20866.1.
BC049625 mRNA. Translation: AAH49625.1.
CCDSiCCDS29130.1. [Q99MH5-1]
RefSeqiNP_542368.2. NM_080637.3. [Q99MH5-1]
UniGeneiMm.275430.

Genome annotation databases

EnsembliENSMUST00000079287; ENSMUSP00000078269; ENSMUSG00000035984. [Q99MH5-1]
ENSMUST00000134875; ENSMUSP00000118213; ENSMUSG00000035984. [Q99MH5-2]
ENSMUST00000154342; ENSMUSP00000117443; ENSMUSG00000035984. [Q99MH5-2]
GeneIDi75533.
KEGGimmu:75533.
UCSCiuc008eks.1. mouse. [Q99MH5-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF343565 mRNA. Translation: AAK20866.1.
BC049625 mRNA. Translation: AAH49625.1.
CCDSiCCDS29130.1. [Q99MH5-1]
RefSeqiNP_542368.2. NM_080637.3. [Q99MH5-1]
UniGeneiMm.275430.

3D structure databases

ProteinModelPortaliQ99MH5.
SMRiQ99MH5. Positions 13-143, 156-198.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000078269.

PTM databases

PhosphoSiteiQ99MH5.

Proteomic databases

MaxQBiQ99MH5.
PaxDbiQ99MH5.
PRIDEiQ99MH5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000079287; ENSMUSP00000078269; ENSMUSG00000035984. [Q99MH5-1]
ENSMUST00000134875; ENSMUSP00000118213; ENSMUSG00000035984. [Q99MH5-2]
ENSMUST00000154342; ENSMUSP00000117443; ENSMUSG00000035984. [Q99MH5-2]
GeneIDi75533.
KEGGimmu:75533.
UCSCiuc008eks.1. mouse. [Q99MH5-1]

Organism-specific databases

CTDi8382.
MGIiMGI:1922783. Nme5.

Phylogenomic databases

eggNOGiKOG0888. Eukaryota.
COG0105. LUCA.
GeneTreeiENSGT00760000119146.
HOGENOMiHOG000224565.
HOVERGENiHBG000198.
InParanoidiQ99MH5.
KOiK00940.
OMAiDFYAEHY.
OrthoDBiEOG7NSB3J.
PhylomeDBiQ99MH5.
TreeFamiTF354225.

Miscellaneous databases

PROiQ99MH5.
SOURCEiSearch...

Gene expression databases

BgeeiQ99MH5.
ExpressionAtlasiQ99MH5. baseline and differential.
GenevisibleiQ99MH5. MM.

Family and domain databases

InterProiIPR007858. Dpy-30_motif.
IPR012410. NDK_H5.
IPR001564. Nucleoside_diP_kinase.
[Graphical view]
PfamiPF05186. Dpy-30. 1 hit.
PF00334. NDK. 1 hit.
[Graphical view]
PIRSFiPIRSF036504. NDK_H5. 1 hit.
PRINTSiPR01243. NUCDPKINASE.
SMARTiSM00562. NDK. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning, sequencing, and characterization of the murine nm23-M5 gene during mouse spermatogenesis and spermiogenesis."
    Hwang K.-C., Ok D.-W., Hong J.-C., Kim M.-O., Kim J.-H.
    Biochem. Biophys. Res. Commun. 306:198-207(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
    Strain: ICR.
    Tissue: Testis.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Tissue: Testis.
  3. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Testis.

Entry informationi

Entry nameiNDK5_MOUSE
AccessioniPrimary (citable) accession number: Q99MH5
Secondary accession number(s): Q810R1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 16, 2001
Last sequence update: July 11, 2003
Last modified: July 6, 2016
This is version 124 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.