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Protein

Nucleoside diphosphate kinase homolog 5

Gene

Nme5

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Does not seem to have NDK kinase activity. Confers protection from cell death by Bax and alters the cellular levels of several antioxidant enzymes including Gpx5. May play a role in spermiogenesis by increasing the ability of late-stage spermatids to eliminate reactive oxygen species.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei127Pros-phosphohistidine intermediateSequence analysis1

GO - Molecular functioni

GO - Biological processi

  • cilium assembly Source: MGI
  • CTP biosynthetic process Source: InterPro
  • epithelial cilium movement Source: MGI
  • GTP biosynthetic process Source: InterPro
  • negative regulation of oxidative stress-induced intrinsic apoptotic signaling pathway Source: MGI
  • response to reactive oxygen species Source: UniProtKB
  • spermatid development Source: UniProtKB
  • UTP biosynthetic process Source: InterPro
  • ventricular system development Source: MGI

Keywordsi

Molecular functionDevelopmental protein
Biological processDifferentiation, Spermatogenesis

Names & Taxonomyi

Protein namesi
Recommended name:
Nucleoside diphosphate kinase homolog 5
Short name:
NDK-H 5
Short name:
NDP kinase homolog 5
Alternative name(s):
nm23-M5
Gene namesi
Name:Nme5
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 18

Organism-specific databases

MGIiMGI:1922783 Nme5

Subcellular locationi

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001371261 – 211Nucleoside diphosphate kinase homolog 5Add BLAST211

Proteomic databases

PaxDbiQ99MH5
PRIDEiQ99MH5

PTM databases

PhosphoSitePlusiQ99MH5

Expressioni

Tissue specificityi

Expressed predominantly in germ cells of the testis. Not expressed in testicular somatic cells.1 Publication

Developmental stagei

First appears in pachytene spermatocytes and increases in abundance in subsequent stages.1 Publication

Gene expression databases

BgeeiENSMUSG00000035984
ExpressionAtlasiQ99MH5 baseline and differential
GenevisibleiQ99MH5 MM

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000078269

Structurei

3D structure databases

ProteinModelPortaliQ99MH5
SMRiQ99MH5
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the NDK family.Curated

Phylogenomic databases

eggNOGiKOG0888 Eukaryota
COG0105 LUCA
GeneTreeiENSGT00760000119146
HOGENOMiHOG000224565
HOVERGENiHBG000198
InParanoidiQ99MH5
KOiK20790
OMAiKLCHHPI
OrthoDBiEOG091G0FHI
PhylomeDBiQ99MH5
TreeFamiTF354225

Family and domain databases

Gene3Di3.30.70.141, 1 hit
InterProiView protein in InterPro
IPR007858 Dpy-30_motif
IPR034907 NDK-like_dom
IPR036850 NDK-like_dom_sf
IPR012410 NDK_H5
IPR001564 Nucleoside_diP_kinase
PfamiView protein in Pfam
PF05186 Dpy-30, 1 hit
PF00334 NDK, 1 hit
PIRSFiPIRSF036504 NDK_H5, 1 hit
PRINTSiPR01243 NUCDPKINASE
SMARTiView protein in SMART
SM00562 NDK, 1 hit
SUPFAMiSSF54919 SSF54919, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Note: Additional isoforms seem to exist.
Isoform 1 (identifier: Q99MH5-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEVSMPLPQI YVEKTLALIK PDVVDKEEEI QDIILGSGFT IIQRRKLHLS
60 70 80 90 100
PEHCSNFYVE QYGKMFFPNL TAYMSSGPLV AMILARHKAI SYWKELMGPS
110 120 130 140 150
NSLVAKETHP DSLRAIYGTD ELRNALHGSN DFAASEREIR FMFPAVIIEP
160 170 180 190 200
IPIGQAAKDY INLYVAPTLL QGLTELCKEK PPDPYLWLAD WLMKNNPNKP
210
KLCHFPVTEE P
Length:211
Mass (Da):24,002
Last modified:July 11, 2003 - v2
Checksum:i3624D0C76BDF3ADD
GO
Isoform 2 (identifier: Q99MH5-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     113-114: LR → DY
     115-211: Missing.

Show »
Length:114
Mass (Da):13,018
Checksum:i29DBC942282C3F02
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti2E → V in AAK20866 (PubMed:12788088).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_007773113 – 114LR → DY in isoform 2. 2 Publications2
Alternative sequenceiVSP_007774115 – 211Missing in isoform 2. 2 PublicationsAdd BLAST97

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF343565 mRNA Translation: AAK20866.1
BC049625 mRNA Translation: AAH49625.1
CCDSiCCDS29130.1 [Q99MH5-1]
RefSeqiNP_542368.2, NM_080637.3 [Q99MH5-1]
XP_017173493.1, XM_017318004.1 [Q99MH5-1]
UniGeneiMm.275430

Genome annotation databases

EnsembliENSMUST00000079287; ENSMUSP00000078269; ENSMUSG00000035984 [Q99MH5-1]
ENSMUST00000134875; ENSMUSP00000118213; ENSMUSG00000035984 [Q99MH5-2]
ENSMUST00000154342; ENSMUSP00000117443; ENSMUSG00000035984 [Q99MH5-2]
GeneIDi75533
KEGGimmu:75533
UCSCiuc008eks.1 mouse [Q99MH5-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiNDK5_MOUSE
AccessioniPrimary (citable) accession number: Q99MH5
Secondary accession number(s): Q810R1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 16, 2001
Last sequence update: July 11, 2003
Last modified: March 28, 2018
This is version 137 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health