Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Nuclear autoantigenic sperm protein

Gene

Nasp

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Required for DNA replication, normal cell cycle progression and cell proliferation. Forms a cytoplasmic complex with HSP90 and linker H1 histones and stimulates HSP90 ATPase activity. NASP and H1 histone are subsequently released from the complex and translocate to the nucleus where the histone is released for binding to DNA.3 Publications

GO - Molecular functioni

  • histone binding Source: MGI
  • Hsp90 protein binding Source: UniProtKB

GO - Biological processi

  • blastocyst development Source: UniProtKB
  • cell cycle Source: UniProtKB
  • cell proliferation Source: UniProtKB
  • DNA replication Source: UniProtKB
  • DNA replication-dependent nucleosome assembly Source: MGI
  • DNA replication-independent nucleosome assembly Source: MGI
  • histone exchange Source: UniProtKB
  • protein transport Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Cell cycle, DNA replication, Protein transport, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Nuclear autoantigenic sperm protein
Short name:
NASP
Gene namesi
Name:NaspImported
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:1355328. Nasp.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB-SubCell
  • nuclear chromatin Source: MGI
  • nucleoplasm Source: MGI
  • nucleus Source: MGI
  • protein complex Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Disruption phenotypei

Mice develop to blastocyst stage, probably as a result of maternally-derived Nasp, and then die.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00002615972 – 773Nuclear autoantigenic sperm proteinAdd BLAST772

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1
Modified residuei33N6-acetyllysineBy similarity1
Modified residuei123PhosphothreonineBy similarity1
Modified residuei127PhosphoserineBy similarity1
Modified residuei169PhosphothreonineBy similarity1
Modified residuei175PhosphoserineBy similarity1
Modified residuei188PhosphoserineBy similarity1
Modified residuei241N6-acetyllysineCombined sources1
Modified residuei242PhosphoserineBy similarity1
Modified residuei251N6-acetyllysineCombined sources1
Modified residuei284N6-acetyllysineCombined sources1
Modified residuei304PhosphoserineBy similarity1
Modified residuei319PhosphoserineBy similarity1
Modified residuei377PhosphothreonineBy similarity1
Modified residuei384PhosphoserineBy similarity1
Modified residuei395PhosphoserineBy similarity1
Modified residuei396PhosphoserineBy similarity1
Modified residuei450PhosphothreonineBy similarity1
Modified residuei463PhosphothreonineCombined sources1
Modified residuei466PhosphoserineCombined sources1
Modified residuei483PhosphoserineBy similarity1
Modified residuei489PhosphoserineBy similarity1
Modified residuei648PhosphoserineBy similarity1
Modified residuei669PhosphothreonineBy similarity1
Modified residuei691PhosphoserineBy similarity1
Modified residuei692PhosphoserineBy similarity1
Modified residuei712PhosphoserineBy similarity1
Cross-linki722Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1)By similarity
Modified residuei731PhosphoserineBy similarity1
Modified residuei737PhosphoserineBy similarity1
Modified residuei742PhosphoserineBy similarity1

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ99MD9.
PaxDbiQ99MD9.
PeptideAtlasiQ99MD9.
PRIDEiQ99MD9.

2D gel databases

REPRODUCTION-2DPAGEQ99MD9.

PTM databases

iPTMnetiQ99MD9.
PhosphoSitePlusiQ99MD9.
SwissPalmiQ99MD9.

Expressioni

Tissue specificityi

Isoform 1 is found in gametes, embryonic cells and transformed cells. Isoform 2 is found in dividing somatic cells (at protein level).1 Publication

Developmental stagei

During the cell cycle, levels increase during S-phase.1 Publication

Gene expression databases

BgeeiENSMUSG00000028693.
ExpressionAtlasiQ99MD9. baseline and differential.
GenevisibleiQ99MD9. MM.

Interactioni

Subunit structurei

Binds to linker H1 histones but not to core histones. Also binds to HSP90 in the cytoplasm. This interaction stimulates binding of NASP to HIST1H1T/H1T.3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
Hist1h1cP158643EBI-913410,EBI-913436

GO - Molecular functioni

  • histone binding Source: MGI
  • Hsp90 protein binding Source: UniProtKB

Protein-protein interaction databases

BioGridi206159. 2 interactors.
IntActiQ99MD9. 8 interactors.
MINTiMINT-4104750.
STRINGi10090.ENSMUSP00000030456.

Structurei

3D structure databases

ProteinModelPortaliQ99MD9.
SMRiQ99MD9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati43 – 76TPR 1Sequence analysisAdd BLAST34
Repeati237 – 270TPR 2Sequence analysisAdd BLAST34
Repeati528 – 561TPR 3Sequence analysisAdd BLAST34
Repeati570 – 603TPR 4Sequence analysisAdd BLAST34

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni116 – 127Histone-bindingBy similarityAdd BLAST12
Regioni210 – 242Histone-bindingBy similarityAdd BLAST33
Regioni455 – 498Histone-bindingBy similarityAdd BLAST44

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili126 – 160Sequence analysisAdd BLAST35
Coiled coili595 – 648Sequence analysisAdd BLAST54

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi702 – 708Nuclear localization signalSequence analysis7

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi111 – 644Glu-richSequence analysisAdd BLAST534

Sequence similaritiesi

Belongs to the NASP family.Curated
Contains 4 TPR repeats.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil, Repeat, TPR repeat

Phylogenomic databases

eggNOGiKOG4563. Eukaryota.
ENOG4110P5E. LUCA.
GeneTreeiENSGT00390000016650.
HOGENOMiHOG000013120.
HOVERGENiHBG002186.
InParanoidiQ99MD9.
KOiK11291.
PhylomeDBiQ99MD9.

Family and domain databases

Gene3Di1.25.40.10. 2 hits.
InterProiIPR019544. Tetratricopeptide_SHNi-TPR_dom.
IPR013026. TPR-contain_dom.
IPR011990. TPR-like_helical_dom.
IPR019734. TPR_repeat.
[Graphical view]
PfamiPF10516. SHNi-TPR. 1 hit.
[Graphical view]
SMARTiSM00028. TPR. 3 hits.
[Graphical view]
SUPFAMiSSF48452. SSF48452. 1 hit.
PROSITEiPS50005. TPR. 3 hits.
PS50293. TPR_REGION. 2 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 12 Publications (identifier: Q99MD9-1) [UniParc]FASTAAdd to basket
Also known as: Testicular NASP1 Publication, tNASP1 Publication

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MATESTAAAA IAAELVSADK IEDAPAPSTS ADKMESLDVD SEAKKLLGLG
60 70 80 90 100
QKHLVMGDIP AAVNAFQEAA SLLGKKYGET ANECGEAFFF YGKSLLELAR
110 120 130 140 150
MENGVLGNAL EGVHVEEEEG EKTEDESLVE NNDNVDEEAR EELREQVYDA
160 170 180 190 200
MGEKEAKKAE GKSLTKPETD KEQESEVEKG GREDMDISEP EEKLQETVEP
210 220 230 240 250
TSKQLTESSE EAKEAAIPGL NEDEVASGKT EQESLCTEKG KSISGAYVQN
260 270 280 290 300
KEFRETVEEG EEIISLEKKP KETSEDQPIR AAEKQGTLMK VVEIEAEIDP
310 320 330 340 350
QVKSADVGGE EPKDQVATSE SELGKAVLME LSGQDVEASP VVAAEAGAEV
360 370 380 390 400
SEKPGQEITV IPNNGPVVGQ STVGDQTPSE PQTSAERLTE TKDGSSVEEV
410 420 430 440 450
KAELVPEQEE AMLPVEESEA AGDGVETKVA QKATEKAPED KFKIAANEET
460 470 480 490 500
PERDEQMKEG EETEGSEEED RENDKAEETP NESVLEKKSL QENEEEEIGN
510 520 530 540 550
LELAWDMLDL AKIIFKRQET KEAQLYAAQA HLKLGEVSVE SENYIQAVEE
560 570 580 590 600
FQACLSLQEQ YLEAHDRLLA ETHYQLGLAY GYNSQYDEAV AQFGKSIDVI
610 620 630 640 650
EKRMAVLHEQ MKEAEGSFTE YEKEIEELKE LLPEIREKIE DAKESQRSGN
660 670 680 690 700
VAELALKATL VESSTSGFTP SGAGASVSMI ASRKPTDGAS SSNCVTDISH
710 720 730 740 750
LVRKKRKPEE ESPRKDDAKK AKQEPEVNGG SGDAVSSGKE VSENMEAEAE
760 770
NQAESQTAEG TVESAATIKS TAC
Length:773
Mass (Da):83,954
Last modified:November 28, 2006 - v2
Checksum:i63C5CCA025972390
GO
Isoform 22 Publications (identifier: Q99MD9-2) [UniParc]FASTAAdd to basket
Also known as: Somatic NASP1 Publication, sNASP1 Publication

The sequence of this isoform differs from the canonical sequence as follows:
     74-100: Missing.
     138-462: Missing.

Show »
Length:421
Mass (Da):45,754
Checksum:i1892256A471A5F19
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti432K → R in BAE21303 (PubMed:16141072).Curated1
Sequence conflicti451P → Q in BAE21303 (PubMed:16141072).Curated1
Sequence conflicti462Missing in AAK31170 (PubMed:16728391).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_05223774 – 100Missing in isoform 2. 3 PublicationsAdd BLAST27
Alternative sequenceiVSP_052238138 – 462Missing in isoform 2. 3 PublicationsAdd BLAST325

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF034610 mRNA. Translation: AAB87567.2.
AF095722 mRNA. Translation: AAC64195.1.
AF349432 Genomic DNA. Translation: AAK31170.1.
AF349432 Genomic DNA. Translation: AAK31171.1.
AK083333 mRNA. Translation: BAC38871.1.
AK132690 mRNA. Translation: BAE21303.1.
BC004693 mRNA. Translation: AAH04693.1.
CCDSiCCDS18513.1. [Q99MD9-1]
CCDS71451.1. [Q99MD9-2]
RefSeqiNP_001074944.1. NM_001081475.1.
NP_001271158.1. NM_001284229.1. [Q99MD9-2]
UniGeneiMm.257181.

Genome annotation databases

EnsembliENSMUST00000081182; ENSMUSP00000079946; ENSMUSG00000028693. [Q99MD9-2]
GeneIDi50927.
KEGGimmu:50927.
UCSCiuc008ugw.1. mouse. [Q99MD9-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF034610 mRNA. Translation: AAB87567.2.
AF095722 mRNA. Translation: AAC64195.1.
AF349432 Genomic DNA. Translation: AAK31170.1.
AF349432 Genomic DNA. Translation: AAK31171.1.
AK083333 mRNA. Translation: BAC38871.1.
AK132690 mRNA. Translation: BAE21303.1.
BC004693 mRNA. Translation: AAH04693.1.
CCDSiCCDS18513.1. [Q99MD9-1]
CCDS71451.1. [Q99MD9-2]
RefSeqiNP_001074944.1. NM_001081475.1.
NP_001271158.1. NM_001284229.1. [Q99MD9-2]
UniGeneiMm.257181.

3D structure databases

ProteinModelPortaliQ99MD9.
SMRiQ99MD9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi206159. 2 interactors.
IntActiQ99MD9. 8 interactors.
MINTiMINT-4104750.
STRINGi10090.ENSMUSP00000030456.

PTM databases

iPTMnetiQ99MD9.
PhosphoSitePlusiQ99MD9.
SwissPalmiQ99MD9.

2D gel databases

REPRODUCTION-2DPAGEQ99MD9.

Proteomic databases

EPDiQ99MD9.
PaxDbiQ99MD9.
PeptideAtlasiQ99MD9.
PRIDEiQ99MD9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000081182; ENSMUSP00000079946; ENSMUSG00000028693. [Q99MD9-2]
GeneIDi50927.
KEGGimmu:50927.
UCSCiuc008ugw.1. mouse. [Q99MD9-2]

Organism-specific databases

CTDi4678.
MGIiMGI:1355328. Nasp.

Phylogenomic databases

eggNOGiKOG4563. Eukaryota.
ENOG4110P5E. LUCA.
GeneTreeiENSGT00390000016650.
HOGENOMiHOG000013120.
HOVERGENiHBG002186.
InParanoidiQ99MD9.
KOiK11291.
PhylomeDBiQ99MD9.

Miscellaneous databases

ChiTaRSiNasp. mouse.
PROiQ99MD9.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000028693.
ExpressionAtlasiQ99MD9. baseline and differential.
GenevisibleiQ99MD9. MM.

Family and domain databases

Gene3Di1.25.40.10. 2 hits.
InterProiIPR019544. Tetratricopeptide_SHNi-TPR_dom.
IPR013026. TPR-contain_dom.
IPR011990. TPR-like_helical_dom.
IPR019734. TPR_repeat.
[Graphical view]
PfamiPF10516. SHNi-TPR. 1 hit.
[Graphical view]
SMARTiSM00028. TPR. 3 hits.
[Graphical view]
SUPFAMiSSF48452. SSF48452. 1 hit.
PROSITEiPS50005. TPR. 3 hits.
PS50293. TPR_REGION. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNASP_MOUSE
AccessioniPrimary (citable) accession number: Q99MD9
Secondary accession number(s): O35499
, O88993, Q3V150, Q99KE9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 28, 2006
Last sequence update: November 28, 2006
Last modified: November 2, 2016
This is version 117 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.