Q99MC0 (PP14A_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein phosphatase 1 regulatory subunit 14A

Alternative name(s):
17 kDa PKC-potentiated inhibitory protein of PP1
Protein kinase C-potentiated inhibitor protein of 17 kDa
Short name=CPI-17

Gene names

Name:	Ppp1r14a
Synonyms:	Cpi, Cpi17

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	147 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Inhibitor of PPP1CA. Has over 1000-fold higher inhibitory activity when phosphorylated, creating a molecular switch for regulating the phosphorylation status of PPP1CA substrates and smooth muscle contraction By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the PP1 inhibitor family.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Protein phosphatase inhibitor
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	regulation of phosphorylation Inferred from electronic annotation. Source: InterPro
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	protein phosphatase inhibitor activity Traceable author statement PubMed 12359219. Source: RGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 147	147	Protein phosphatase 1 regulatory subunit 14A		PRO_0000071489
Regions
	Region	35 – 120	86	Inhibitory
Amino acid modifications
	Modified residue	26	1	Phosphoserine By similarity
	Modified residue	38	1	Phosphothreonine By similarity
	Modified residue	128	1	Phosphoserine By similarity
	Modified residue	130	1	Phosphoserine By similarity
	Modified residue	134	1	Phosphoserine By similarity
	Modified residue	136	1	Phosphoserine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q99MC0 [UniParc]. Last modified June 1, 2001. Version 1. Checksum: 116B39AA2FA1CD12	FASTA	147	16,697
	10 20 30 40 50 60 MAAQRLGKRV LSKLQSPSRA RGPGGSPSGL QKRHARVTVK YDRRELQRRL DVEKWIDGRL 70 80 90 100 110 120 EELYRGREAD MPDEVNIDEL LELDSEEERC RKIRGLLEAC LNPTEDFVQE LLAKLRGLHK 130 140 QPGFPQPSPS DDPSLSPRQD PAHTAPP

References

[1]	Yu L. Submitted (FEB-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"GBPI, a novel gastrointestinal- and brain-specific PP1-inhibitory protein, is activated by PKC and inactivated by PKA." Liu Q.-R., Zhang P.-W., Lin Z., Li Q.-F., Woods A.S., Troncoso J., Uhl G.R. Biochem. J. 377:171-181(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Smooth muscle.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF352572 mRNA. Translation: AAK35213.1. AY050673 mRNA. Translation: AAL25831.1.
IPI	IPI00197727.
RefSeq	NP_569087.1. NM_130403.1.
UniGene	Rn.73852.
3D structure databases
ProteinModelPortal	Q99MC0.
SMR	Q99MC0. Positions 22-120.
ModBase	Search...
Protein-protein interaction databases
STRING	10116.ENSRNOP00000028070.
PTM databases
PhosphoSite	Q99MC0.
Proteomic databases
PaxDb	Q99MC0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000028070; ENSRNOP00000028070; ENSRNOG00000020676.
GeneID	114004.
KEGG	rno:114004.
UCSC	RGD:620536. rat.
Organism-specific databases
CTD	94274.
RGD	620536. Ppp1r14a.
Phylogenomic databases
eggNOG	NOG78020.
GeneTree	ENSGT00390000003608.
HOGENOM	HOG000231993.
HOVERGEN	HBG054419.
InParanoid	Q99MC0.
KO	K12328.
OMA	LHKQPGF.
OrthoDB	EOG4HDSW5.
Gene expression databases
Genevestigator	Q99MC0.
GermOnline	ENSRNOG00000020676. Rattus norvegicus.
Family and domain databases
Gene3D	1.10.150.220. 1 hit.
InterPro	IPR008025. PP1_inhibitor. [Graphical view]
PANTHER	PTHR16188. PTHR16188. 1 hit.
Pfam	PF05361. PP1_inhibitor. 1 hit. [Graphical view]
SUPFAM	SSF81790. PP1_inhibitor. 1 hit.
ProtoNet	Search...
Other
NextBio	618150.

Entry information

Entry name

PP14A_RAT

Accession

Primary (citable) accession number: Q99MC0

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 9, 2004
Last sequence update:	June 1, 2001
Last modified:	April 3, 2013
This is version 74 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program