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Q99MA9

- NKX61_MOUSE

UniProt

Q99MA9 - NKX61_MOUSE

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Protein

Homeobox protein Nkx-6.1

Gene

Nkx6-1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Transcription factor which binds to specific A/T-rich DNA sequences in the promoter regions of a number of genes. Involved in transcriptional regulation in islet beta cells. Binds to the insulin promoter and is involved in regulation of the insulin gene. Together with NKX2-2 and IRX3 acts to restrict the generation of motor neurons to the appropriate region of the neural tube. Belongs to the class II proteins of neuronal progenitor factors, which are induced by SHH signals.1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi237 – 29660HomeoboxPROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. chromatin binding Source: Ensembl
  2. sequence-specific DNA binding Source: Ensembl
  3. sequence-specific DNA binding transcription factor activity Source: MGI

GO - Biological processi

  1. cell differentiation Source: MGI
  2. cell proliferation Source: Ensembl
  3. cellular response to cytokine stimulus Source: Ensembl
  4. cellular response to peptide hormone stimulus Source: Ensembl
  5. central nervous system neuron differentiation Source: MGI
  6. endocrine pancreas development Source: MGI
  7. negative regulation of glial cell differentiation Source: MGI
  8. oligodendrocyte differentiation Source: MGI
  9. positive regulation of glial cell differentiation Source: MGI
  10. positive regulation of insulin secretion Source: Ensembl
  11. positive regulation of neuron differentiation Source: MGI
  12. regulation of axon extension Source: MGI
  13. regulation of neuron migration Source: MGI
  14. regulation of transcription from RNA polymerase II promoter Source: MGI
  15. regulation of transcription from RNA polymerase II promoter involved in spinal cord motor neuron fate specification Source: MGI
  16. regulation of transcription from RNA polymerase II promoter involved in ventral spinal cord interneuron specification Source: MGI
  17. response to drug Source: Ensembl
  18. response to nicotine Source: Ensembl
  19. smoothened signaling pathway Source: MGI
  20. transcription from RNA polymerase II promoter Source: Ensembl
  21. type B pancreatic cell development Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

ReactomeiREACT_208465. Regulation of gene expression in early pancreatic precursor cells.
REACT_214529. Regulation of gene expression in beta cells.

Names & Taxonomyi

Protein namesi
Recommended name:
Homeobox protein Nkx-6.1
Alternative name(s):
Homeobox protein NK-6 homolog A
Gene namesi
Name:Nkx6-1
Synonyms:Nkx6.1, Nkx6a
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 5

Organism-specific databases

MGIiMGI:1206039. Nkx6-1.

Subcellular locationi

GO - Cellular componenti

  1. cytosol Source: Reactome
  2. extracellular vesicular exosome Source: Ensembl
  3. nucleus Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

Dramatically diminished numbers of beta cells within the pancreatic islets and secretion of insulin at only 2% of normal levels. Loss of expression of Mafa, which is involved in insulin gene transcription.2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 365365Homeobox protein Nkx-6.1PRO_0000048953Add
BLAST

Proteomic databases

PRIDEiQ99MA9.

PTM databases

PhosphoSiteiQ99MA9.

Expressioni

Tissue specificityi

Expressed by neuronal progenitor cells in discrete domains of the ventral neural tube.1 Publication

Gene expression databases

BgeeiQ99MA9.
GenevestigatoriQ99MA9.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000042716.

Structurei

3D structure databases

ProteinModelPortaliQ99MA9.
SMRiQ99MA9. Positions 244-294.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni102 – 269168Repressor domainBy similarityAdd
BLAST
Regioni307 – 36559Involved in DNA-bindingBy similarityAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi49 – 6113Poly-SerAdd
BLAST
Compositional biasi119 – 13214Poly-SerAdd
BLAST
Compositional biasi136 – 15116Poly-AlaAdd
BLAST
Compositional biasi169 – 1746Poly-Pro
Compositional biasi323 – 3264Poly-Asp

Domaini

The C-terminal domain contributes to sequence-specific DNA-binding.

Sequence similaritiesi

Contains 1 homeobox DNA-binding domain.PROSITE-ProRule annotation

Keywords - Domaini

Homeobox

Phylogenomic databases

eggNOGiNOG265304.
GeneTreeiENSGT00760000118779.
HOGENOMiHOG000060127.
HOVERGENiHBG070987.
InParanoidiQ99MA9.
KOiK08030.
OMAiTPLYPAY.
OrthoDBiEOG7Z95QB.
PhylomeDBiQ99MA9.
TreeFamiTF327063.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
InterProiIPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR020479. Homeobox_metazoa.
IPR009057. Homeodomain-like.
IPR000047. HTH_motif.
[Graphical view]
PfamiPF00046. Homeobox. 1 hit.
[Graphical view]
PRINTSiPR00024. HOMEOBOX.
PR00031. HTHREPRESSR.
SMARTiSM00389. HOX. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
PROSITEiPS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q99MA9-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MLAVGAMEGP RQSAFLLSSP PLAALHSMAE MKTPLYPAAY PPLPTGPPSS
60 70 80 90 100
SSSSSSSSSP SPPLGSHNPG GLKPPAAGGL SSLGSPPQQL SAATPHGIND
110 120 130 140 150
ILSRPSMPVA SGAALPSASP SGSSSSSSSS ASATSASAAA AAAAAAAAAA
160 170 180 190 200
ASSPAGLLAG LPRFSSLSPP PPPPGLYFSP SAAAVAAVGR YPKPLAELPG
210 220 230 240 250
RTPIFWPGVM QSPPWRDARL ACTPHQGSIL LDKDGKRKHT RPTFSGQQIF
260 270 280 290 300
ALEKTFEQTK YLAGPERARL AYSLGMTESQ VKVWFQNRRT KWRKKHAAEM
310 320 330 340 350
ATAKKKQDSE TERLKGTSEN EEDDDDYNKP LDPNSDDEKI TQLLKKHKSS
360
GGSLLLHASE AEGSS
Length:365
Mass (Da):37,705
Last modified:June 1, 2001 - v1
Checksum:iB6AABAF5748A99E8
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF357883 mRNA. Translation: AAK37567.1.
BC138019 mRNA. Translation: AAI38020.1.
BC138020 mRNA. Translation: AAI38021.1.
AF291666 Genomic DNA. Translation: AAG30415.1.
CCDSiCCDS19471.1.
RefSeqiNP_659204.1. NM_144955.2.
UniGeneiMm.193072.

Genome annotation databases

EnsembliENSMUST00000044125; ENSMUSP00000042716; ENSMUSG00000035187.
GeneIDi18096.
KEGGimmu:18096.
UCSCiuc008yim.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF357883 mRNA. Translation: AAK37567.1 .
BC138019 mRNA. Translation: AAI38020.1 .
BC138020 mRNA. Translation: AAI38021.1 .
AF291666 Genomic DNA. Translation: AAG30415.1 .
CCDSi CCDS19471.1.
RefSeqi NP_659204.1. NM_144955.2.
UniGenei Mm.193072.

3D structure databases

ProteinModelPortali Q99MA9.
SMRi Q99MA9. Positions 244-294.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 10090.ENSMUSP00000042716.

PTM databases

PhosphoSitei Q99MA9.

Proteomic databases

PRIDEi Q99MA9.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000044125 ; ENSMUSP00000042716 ; ENSMUSG00000035187 .
GeneIDi 18096.
KEGGi mmu:18096.
UCSCi uc008yim.1. mouse.

Organism-specific databases

CTDi 4825.
MGIi MGI:1206039. Nkx6-1.

Phylogenomic databases

eggNOGi NOG265304.
GeneTreei ENSGT00760000118779.
HOGENOMi HOG000060127.
HOVERGENi HBG070987.
InParanoidi Q99MA9.
KOi K08030.
OMAi TPLYPAY.
OrthoDBi EOG7Z95QB.
PhylomeDBi Q99MA9.
TreeFami TF327063.

Enzyme and pathway databases

Reactomei REACT_208465. Regulation of gene expression in early pancreatic precursor cells.
REACT_214529. Regulation of gene expression in beta cells.

Miscellaneous databases

NextBioi 293251.
PROi Q99MA9.
SOURCEi Search...

Gene expression databases

Bgeei Q99MA9.
Genevestigatori Q99MA9.

Family and domain databases

Gene3Di 1.10.10.60. 1 hit.
InterProi IPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR020479. Homeobox_metazoa.
IPR009057. Homeodomain-like.
IPR000047. HTH_motif.
[Graphical view ]
Pfami PF00046. Homeobox. 1 hit.
[Graphical view ]
PRINTSi PR00024. HOMEOBOX.
PR00031. HTHREPRESSR.
SMARTi SM00389. HOX. 1 hit.
[Graphical view ]
SUPFAMi SSF46689. SSF46689. 1 hit.
PROSITEi PS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Isolation of partial cDNA sequence for the mouse homeodomain protein, Nkx6.1."
    Sander M., Nelson S.B.
    Submitted (MAR-2001) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: C57BL/6 X DBA/2.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Brain.
  3. "Transcriptional and translational regulation of beta-cell differentiation factor Nkx6.1."
    Watada H., Mirmira R.G., Leung J., German M.S.
    J. Biol. Chem. 275:34224-34230(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-224.
    Strain: 129/Sv.
  4. "Homeobox gene Nkx6.1 lies downstream of Nkx2.2 in the major pathway of beta-cell formation in the pancreas."
    Sander M., Sussel L., Conners J., Scheel D., Kalamaras J., Dela Cruz F., Schwitzgebel V., Hayes-Jordan A., German M.
    Development 127:5533-5540(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISRUPTION PHENOTYPE.
  5. "A homeodomain protein code specifies progenitor cell identity and neuronal fate in the ventral neural tube."
    Briscoe J., Pierani A., Jessell T.M., Ericson J.
    Cell 101:435-445(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN NEURAL PATTERNING, TISSUE SPECIFICITY.
  6. "The MafA transcription factor appears to be responsible for tissue-specific expression of insulin."
    Matsuoka T.A., Artner I., Henderson E., Means A., Sander M., Stein R.
    Proc. Natl. Acad. Sci. U.S.A. 101:2930-2933(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISRUPTION PHENOTYPE.

Entry informationi

Entry nameiNKX61_MOUSE
AccessioniPrimary (citable) accession number: Q99MA9
Secondary accession number(s): B2RQP4, Q9ERQ7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 10, 2003
Last sequence update: June 1, 2001
Last modified: October 29, 2014
This is version 101 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3