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Q99MA9 (NKX61_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 98. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Homeobox protein Nkx-6.1
Alternative name(s):
Homeobox protein NK-6 homolog A
Gene names
Name:Nkx6-1
Synonyms:Nkx6.1, Nkx6a
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length365 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Transcription factor which binds to specific A/T-rich DNA sequences in the promoter regions of a number of genes. Involved in transcriptional regulation in islet beta cells. Binds to the insulin promoter and is involved in regulation of the insulin gene. Together with NKX2-2 and IRX3 acts to restrict the generation of motor neurons to the appropriate region of the neural tube. Belongs to the class II proteins of neuronal progenitor factors, which are induced by SHH signals. Ref.5

Subcellular location

Nucleus.

Tissue specificity

Expressed by neuronal progenitor cells in discrete domains of the ventral neural tube. Ref.5

Domain

The C-terminal domain contributes to sequence-specific DNA-binding.

Disruption phenotype

Dramatically diminished numbers of beta cells within the pancreatic islets and secretion of insulin at only 2% of normal levels. Loss of expression of Mafa, which is involved in insulin gene transcription. Ref.4 Ref.6

Sequence similarities

Contains 1 homeobox DNA-binding domain.

Ontologies

Keywords
   Cellular componentNucleus
   DomainHomeobox
   LigandDNA-binding
   Molecular functionDevelopmental protein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processcell differentiation

Inferred from mutant phenotype PubMed 15944193. Source: MGI

cell proliferation

Inferred from electronic annotation. Source: Ensembl

cellular response to cytokine stimulus

Inferred from electronic annotation. Source: Ensembl

cellular response to peptide hormone stimulus

Inferred from electronic annotation. Source: Ensembl

endocrine pancreas development

Inferred from mutant phenotype PubMed 15944193. Source: MGI

negative regulation of glial cell differentiation

Inferred from genetic interaction PubMed 15629702. Source: MGI

oligodendrocyte differentiation

Inferred from genetic interaction PubMed 15629701. Source: MGI

positive regulation of glial cell differentiation

Inferred from genetic interaction PubMed 15629702. Source: MGI

positive regulation of insulin secretion

Inferred from electronic annotation. Source: Ensembl

positive regulation of neuron differentiation

Inferred from direct assay Ref.5. Source: MGI

regulation of transcription from RNA polymerase II promoter

Inferred from mutant phenotype PubMed 20081190. Source: MGI

regulation of transcription from RNA polymerase II promoter involved in spinal cord motor neuron fate specification

Inferred from genetic interaction PubMed 11567614. Source: MGI

regulation of transcription from RNA polymerase II promoter involved in ventral spinal cord interneuron specification

Inferred from mutant phenotype PubMed 11567614. Source: MGI

response to drug

Inferred from electronic annotation. Source: Ensembl

response to nicotine

Inferred from electronic annotation. Source: Ensembl

smoothened signaling pathway

Inferred from direct assay PubMed 18590716. Source: MGI

transcription from RNA polymerase II promoter

Inferred from electronic annotation. Source: Ensembl

type B pancreatic cell development

Inferred from electronic annotation. Source: Ensembl

   Cellular_componentcytosol

Traceable author statement. Source: Reactome

nucleus

Inferred from direct assay PubMed 14573534PubMed 16306355PubMed 18076286. Source: MGI

   Molecular_functionchromatin binding

Inferred from electronic annotation. Source: Ensembl

sequence-specific DNA binding transcription factor activity

Traceable author statement Ref.3. Source: MGI

transcription regulatory region sequence-specific DNA binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 365365Homeobox protein Nkx-6.1
PRO_0000048953

Regions

DNA binding237 – 29660Homeobox
Region102 – 269168Repressor domain By similarity
Region307 – 36559Involved in DNA-binding By similarity
Compositional bias49 – 6113Poly-Ser
Compositional bias119 – 13214Poly-Ser
Compositional bias136 – 15116Poly-Ala
Compositional bias169 – 1746Poly-Pro
Compositional bias323 – 3264Poly-Asp

Sequences

Sequence LengthMass (Da)Tools
Q99MA9 [UniParc].

Last modified June 1, 2001. Version 1.
Checksum: B6AABAF5748A99E8

FASTA36537,705
        10         20         30         40         50         60 
MLAVGAMEGP RQSAFLLSSP PLAALHSMAE MKTPLYPAAY PPLPTGPPSS SSSSSSSSSP 

        70         80         90        100        110        120 
SPPLGSHNPG GLKPPAAGGL SSLGSPPQQL SAATPHGIND ILSRPSMPVA SGAALPSASP 

       130        140        150        160        170        180 
SGSSSSSSSS ASATSASAAA AAAAAAAAAA ASSPAGLLAG LPRFSSLSPP PPPPGLYFSP 

       190        200        210        220        230        240 
SAAAVAAVGR YPKPLAELPG RTPIFWPGVM QSPPWRDARL ACTPHQGSIL LDKDGKRKHT 

       250        260        270        280        290        300 
RPTFSGQQIF ALEKTFEQTK YLAGPERARL AYSLGMTESQ VKVWFQNRRT KWRKKHAAEM 

       310        320        330        340        350        360 
ATAKKKQDSE TERLKGTSEN EEDDDDYNKP LDPNSDDEKI TQLLKKHKSS GGSLLLHASE 


AEGSS 

« Hide

References

« Hide 'large scale' references
[1]"Isolation of partial cDNA sequence for the mouse homeodomain protein, Nkx6.1."
Sander M., Nelson S.B.
Submitted (MAR-2001) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: C57BL/6 X DBA/2.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Brain.
[3]"Transcriptional and translational regulation of beta-cell differentiation factor Nkx6.1."
Watada H., Mirmira R.G., Leung J., German M.S.
J. Biol. Chem. 275:34224-34230(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-224.
Strain: 129/Sv.
[4]"Homeobox gene Nkx6.1 lies downstream of Nkx2.2 in the major pathway of beta-cell formation in the pancreas."
Sander M., Sussel L., Conners J., Scheel D., Kalamaras J., Dela Cruz F., Schwitzgebel V., Hayes-Jordan A., German M.
Development 127:5533-5540(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: DISRUPTION PHENOTYPE.
[5]"A homeodomain protein code specifies progenitor cell identity and neuronal fate in the ventral neural tube."
Briscoe J., Pierani A., Jessell T.M., Ericson J.
Cell 101:435-445(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION IN NEURAL PATTERNING, TISSUE SPECIFICITY.
[6]"The MafA transcription factor appears to be responsible for tissue-specific expression of insulin."
Matsuoka T.A., Artner I., Henderson E., Means A., Sander M., Stein R.
Proc. Natl. Acad. Sci. U.S.A. 101:2930-2933(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: DISRUPTION PHENOTYPE.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF357883 mRNA. Translation: AAK37567.1.
BC138019 mRNA. Translation: AAI38020.1.
BC138020 mRNA. Translation: AAI38021.1.
AF291666 Genomic DNA. Translation: AAG30415.1.
CCDSCCDS19471.1.
RefSeqNP_659204.1. NM_144955.2.
UniGeneMm.193072.

3D structure databases

ProteinModelPortalQ99MA9.
SMRQ99MA9. Positions 244-294.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10090.ENSMUSP00000042716.

PTM databases

PhosphoSiteQ99MA9.

Proteomic databases

PRIDEQ99MA9.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000044125; ENSMUSP00000042716; ENSMUSG00000035187.
GeneID18096.
KEGGmmu:18096.
UCSCuc008yim.1. mouse.

Organism-specific databases

CTD4825.
MGIMGI:1206039. Nkx6-1.

Phylogenomic databases

eggNOGNOG265304.
GeneTreeENSGT00750000117350.
HOGENOMHOG000060127.
HOVERGENHBG070987.
InParanoidB2RQP4.
KOK08030.
OMATPLYPAY.
OrthoDBEOG7Z95QB.
PhylomeDBQ99MA9.
TreeFamTF327063.

Enzyme and pathway databases

ReactomeREACT_13641. Regulation of Beta-Cell Development.
REACT_188576. Developmental Biology.

Gene expression databases

BgeeQ99MA9.
GenevestigatorQ99MA9.

Family and domain databases

Gene3D1.10.10.60. 1 hit.
InterProIPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR020479. Homeobox_metazoa.
IPR009057. Homeodomain-like.
IPR000047. HTH_motif.
[Graphical view]
PfamPF00046. Homeobox. 1 hit.
[Graphical view]
PRINTSPR00024. HOMEOBOX.
PR00031. HTHREPRESSR.
SMARTSM00389. HOX. 1 hit.
[Graphical view]
SUPFAMSSF46689. SSF46689. 1 hit.
PROSITEPS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio293251.
PROQ99MA9.
SOURCESearch...

Entry information

Entry nameNKX61_MOUSE
AccessionPrimary (citable) accession number: Q99MA9
Secondary accession number(s): B2RQP4, Q9ERQ7
Entry history
Integrated into UniProtKB/Swiss-Prot: January 10, 2003
Last sequence update: June 1, 2001
Last modified: July 9, 2014
This is version 98 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot