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Protein

DnaJ homolog subfamily A member 3, mitochondrial

Gene

Dnaja3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Modulates apoptotic signal transduction or effector structures within the mitochondrial matrix. Affect cytochrome C release from the mitochondria and caspase 3 activation, but not caspase 8 activation. Isoform 1 increases apoptosis triggered by both TNF and the DNA-damaging agent mytomycin C; in sharp contrast, isoform 2 suppresses apoptosis. Can modulate IFN-gamma-mediated transcriptional activity (By similarity). Isoform 2 may play a role in neuromuscular junction development as an effector of the MUSK signaling pathway.By similarity1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi236Zinc 1By similarity1
Metal bindingi239Zinc 1By similarity1
Metal bindingi253Zinc 2By similarity1
Metal bindingi256Zinc 2By similarity1
Metal bindingi275Zinc 2By similarity1
Metal bindingi278Zinc 2By similarity1
Metal bindingi289Zinc 1By similarity1
Metal bindingi292Zinc 1By similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri223 – 301CR-typeAdd BLAST79

GO - Molecular functioni

  • ATP binding Source: InterPro
  • GTPase regulator activity Source: MGI
  • metal ion binding Source: UniProtKB-KW
  • receptor tyrosine kinase binding Source: UniProtKB
  • unfolded protein binding Source: MGI

GO - Biological processi

  • activation-induced cell death of T cells Source: MGI
  • apoptotic process Source: MGI
  • cell aging Source: MGI
  • mitochondrial DNA replication Source: MGI
  • mitochondrion organization Source: MGI
  • negative regulation of cell proliferation Source: MGI
  • negative regulation of NF-kappaB transcription factor activity Source: MGI
  • negative regulation of programmed cell death Source: MGI
  • negative regulation of transcription from RNA polymerase II promoter Source: MGI
  • neuromuscular junction development Source: UniProtKB
  • positive regulation of T cell proliferation Source: MGI
  • protein folding Source: InterPro
  • regulation of apoptotic process Source: GO_Central
  • response to heat Source: InterPro
  • skeletal muscle acetylcholine-gated channel clustering Source: UniProtKB
  • small GTPase mediated signal transduction Source: MGI
  • T cell differentiation in thymus Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Chaperone

Keywords - Biological processi

Apoptosis

Keywords - Ligandi

Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
DnaJ homolog subfamily A member 3, mitochondrial
Alternative name(s):
DnaJ protein Tid-1
Short name:
mTid-1
Tumorous imaginal discs protein Tid56 homolog
Gene namesi
Name:Dnaja3
Synonyms:Tid1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 16

Organism-specific databases

MGIiMGI:1933786. Dnaja3.

Subcellular locationi

  • Mitochondrion matrix By similarity
  • Cytoplasmcytosol 1 Publication
  • Cell junctionsynapsepostsynaptic cell membrane 1 Publication; Peripheral membrane protein 1 Publication

  • Note: Recruited to the postsynaptic cell membrane of the neuromuscular junction through interaction with MUSK.

GO - Cellular componenti

  • actin filament Source: Ensembl
  • cell junction Source: UniProtKB-KW
  • cytosol Source: UniProtKB
  • extrinsic component of plasma membrane Source: UniProtKB
  • intracellular membrane-bounded organelle Source: MGI
  • mitochondrial matrix Source: MGI
  • mitochondrion Source: MGI
  • neuromuscular junction Source: UniProtKB
  • nucleus Source: MGI
  • plasma membrane Source: GOC
  • postsynaptic membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Cytoplasm, Membrane, Mitochondrion, Postsynaptic cell membrane, Synapse

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_0000007257? – 480DnaJ homolog subfamily A member 3, mitochondrial
Transit peptidei1 – ?MitochondrionSequence analysis

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei58Omega-N-methylarginine; by CARM1By similarity1
Modified residuei134N6-acetyllysineCombined sources1
Modified residuei238Omega-N-methylarginine; by CARM1By similarity1
Modified residuei293Omega-N-methylarginine; by CARM1By similarity1
Modified residuei398PhosphoserineBy similarity1

Post-translational modificationi

Tyrosine phosphorylated.

Keywords - PTMi

Acetylation, Methylation, Phosphoprotein

Proteomic databases

PaxDbiQ99M87.
PeptideAtlasiQ99M87.
PRIDEiQ99M87.

PTM databases

iPTMnetiQ99M87.
PhosphoSitePlusiQ99M87.

Expressioni

Gene expression databases

BgeeiENSMUSG00000004069.
CleanExiMM_DNAJA3.
ExpressionAtlasiQ99M87. baseline and differential.
GenevisibleiQ99M87. MM.

Interactioni

Subunit structurei

Interacts with JAK2, HSPA9B and IFN-gammaR2 chain. Interacts with Ras GTPase-activating protein 1 (RASA1). Isoform 2 interacts with MUSK (via the cytoplasmic domain).2 Publications

GO - Molecular functioni

  • receptor tyrosine kinase binding Source: UniProtKB
  • unfolded protein binding Source: MGI

Protein-protein interaction databases

BioGridi219986. 4 interactors.
IntActiQ99M87. 5 interactors.
MINTiMINT-1847235.
STRINGi10090.ENSMUSP00000053842.

Structurei

3D structure databases

ProteinModelPortaliQ99M87.
SMRiQ99M87.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini93 – 158JAdd BLAST66
Repeati236 – 243CXXCXGXG motif8
Repeati253 – 260CXXCXGXG motif8
Repeati275 – 282CXXCXGXG motif8
Repeati289 – 296CXXCXGXG motif8

Domaini

Modulation of apoptosis, i.e. proapoptotic activity of isoform 1 and antiapoptotic activity of isoform 2, is J domain-dependent (By similarity).By similarity

Sequence similaritiesi

Contains 1 CR-type zinc finger.Curated
Contains 1 J domain.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri223 – 301CR-typeAdd BLAST79

Keywords - Domaini

Repeat, Transit peptide, Zinc-finger

Phylogenomic databases

eggNOGiKOG0715. Eukaryota.
COG0484. LUCA.
GeneTreeiENSGT00860000133735.
HOVERGENiHBG051371.
InParanoidiQ99M87.
KOiK09504.
OMAiGPEGFSQ.
OrthoDBiEOG091G09C7.
PhylomeDBiQ99M87.
TreeFamiTF105152.

Family and domain databases

CDDicd06257. DnaJ. 1 hit.
Gene3Di1.10.287.110. 1 hit.
2.10.230.10. 1 hit.
HAMAPiMF_01152. DnaJ. 1 hit.
InterProiIPR012724. DnaJ.
IPR002939. DnaJ_C.
IPR001623. DnaJ_domain.
IPR018253. DnaJ_domain_CS.
IPR008971. HSP40/DnaJ_pept-bd.
IPR001305. HSP_DnaJ_Cys-rich_dom.
[Graphical view]
PfamiPF00226. DnaJ. 1 hit.
PF01556. DnaJ_C. 1 hit.
PF00684. DnaJ_CXXCXGXG. 1 hit.
[Graphical view]
PRINTSiPR00625. JDOMAIN.
SMARTiSM00271. DnaJ. 1 hit.
[Graphical view]
SUPFAMiSSF46565. SSF46565. 1 hit.
SSF49493. SSF49493. 3 hits.
SSF57938. SSF57938. 1 hit.
PROSITEiPS00636. DNAJ_1. 1 hit.
PS50076. DNAJ_2. 1 hit.
PS51188. ZF_CR. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q99M87-1) [UniParc]FASTAAdd to basket
Also known as: Tid-1L, TID1L

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAAWCSPRWL RVAVGTPRLP AAAGRGVQQP QGGVVATSLC RKLCVSAFGL
60 70 80 90 100
SMGAHGPRAL LTLRPGVRLT GTKSFPFVCT TSFHTSASLA KDDYYQILGV
110 120 130 140 150
PRNASQKDIK KAYYQLAKKY HPDTNKDDPK AKEKFSQLAE AYEVLSDEVK
160 170 180 190 200
RKQYDAYGSA GFDPGTSSSG QGYWRGGPSV DPEELFRKIF GEFSSSPFGD
210 220 230 240 250
FQNVFDQPQE YIMELTFNQA AKGVNKEFTV NIMDTCERCD GKGNEPGTKV
260 270 280 290 300
QHCHYCGGSG METINTGPFV MRSTCRRCGG RGSIITNPCV VCRGAGQAKQ
310 320 330 340 350
KKRVTIPVPA GVEDGQTVRM PVGKREIFVT FRVQKSPVFR RDGADIHSDL
360 370 380 390 400
FISIAQAILG GTAKAQGLYE TINVTIPAGI QTDQKIRLTG KGIPRINSYG
410 420 430 440 450
YGDHYIHIKI RVPKRLSSRQ QNLILSYAED ETDVEGTVNG VTHTSTGGRT
460 470 480
MDSSAGSKDR REAGEDNEGF LSKLKKIFTS
Length:480
Mass (Da):52,443
Last modified:June 1, 2001 - v1
Checksum:i30AA5557C18665A8
GO
Isoform 2 (identifier: Q99M87-2) [UniParc]FASTAAdd to basket
Also known as: Tid-1S, TID1S

The sequence of this isoform differs from the canonical sequence as follows:
     448-453: GRTMDS → KRSTGN
     454-480: Missing.

Show »
Length:453
Mass (Da):49,486
Checksum:iA5798CD5C8071EA7
GO
Isoform 3 (identifier: Q99M87-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     211-261: Missing.

Show »
Length:429
Mass (Da):46,847
Checksum:iF549A184D4EFD96F
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti403D → H in BAB23661 (PubMed:16141072).Curated1
Sequence conflicti456G → E in BAB23384 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_007440211 – 261Missing in isoform 3. 1 PublicationAdd BLAST51
Alternative sequenceiVSP_007427448 – 453GRTMDS → KRSTGN in isoform 2. 3 Publications6
Alternative sequenceiVSP_007428454 – 480Missing in isoform 2. 3 PublicationsAdd BLAST27

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY009320 mRNA. Translation: AAG37303.1.
AF325535 mRNA. Translation: AAK11222.1.
AF326358 mRNA. Translation: AAK11223.1.
AK004575 mRNA. Translation: BAB23384.1.
AK004910 mRNA. Translation: BAB23661.1.
AK011535 mRNA. Translation: BAB27682.2.
AK031250 mRNA. Translation: BAC27321.1.
BC003920 mRNA. Translation: AAH03920.1.
BC027240 mRNA. Translation: AAH27240.1.
CCDSiCCDS27922.1. [Q99M87-1]
CCDS49748.1. [Q99M87-2]
RefSeqiNP_001128584.1. NM_001135112.1. [Q99M87-2]
NP_076135.3. NM_023646.4. [Q99M87-1]
UniGeneiMm.248337.

Genome annotation databases

EnsembliENSMUST00000060067; ENSMUSP00000053842; ENSMUSG00000004069. [Q99M87-1]
ENSMUST00000115854; ENSMUSP00000111520; ENSMUSG00000004069. [Q99M87-2]
GeneIDi83945.
KEGGimmu:83945.
UCSCiuc007yac.2. mouse. [Q99M87-1]
uc012aat.1. mouse. [Q99M87-3]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY009320 mRNA. Translation: AAG37303.1.
AF325535 mRNA. Translation: AAK11222.1.
AF326358 mRNA. Translation: AAK11223.1.
AK004575 mRNA. Translation: BAB23384.1.
AK004910 mRNA. Translation: BAB23661.1.
AK011535 mRNA. Translation: BAB27682.2.
AK031250 mRNA. Translation: BAC27321.1.
BC003920 mRNA. Translation: AAH03920.1.
BC027240 mRNA. Translation: AAH27240.1.
CCDSiCCDS27922.1. [Q99M87-1]
CCDS49748.1. [Q99M87-2]
RefSeqiNP_001128584.1. NM_001135112.1. [Q99M87-2]
NP_076135.3. NM_023646.4. [Q99M87-1]
UniGeneiMm.248337.

3D structure databases

ProteinModelPortaliQ99M87.
SMRiQ99M87.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi219986. 4 interactors.
IntActiQ99M87. 5 interactors.
MINTiMINT-1847235.
STRINGi10090.ENSMUSP00000053842.

PTM databases

iPTMnetiQ99M87.
PhosphoSitePlusiQ99M87.

Proteomic databases

PaxDbiQ99M87.
PeptideAtlasiQ99M87.
PRIDEiQ99M87.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000060067; ENSMUSP00000053842; ENSMUSG00000004069. [Q99M87-1]
ENSMUST00000115854; ENSMUSP00000111520; ENSMUSG00000004069. [Q99M87-2]
GeneIDi83945.
KEGGimmu:83945.
UCSCiuc007yac.2. mouse. [Q99M87-1]
uc012aat.1. mouse. [Q99M87-3]

Organism-specific databases

CTDi9093.
MGIiMGI:1933786. Dnaja3.

Phylogenomic databases

eggNOGiKOG0715. Eukaryota.
COG0484. LUCA.
GeneTreeiENSGT00860000133735.
HOVERGENiHBG051371.
InParanoidiQ99M87.
KOiK09504.
OMAiGPEGFSQ.
OrthoDBiEOG091G09C7.
PhylomeDBiQ99M87.
TreeFamiTF105152.

Miscellaneous databases

ChiTaRSiDnaja3. mouse.
PROiQ99M87.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000004069.
CleanExiMM_DNAJA3.
ExpressionAtlasiQ99M87. baseline and differential.
GenevisibleiQ99M87. MM.

Family and domain databases

CDDicd06257. DnaJ. 1 hit.
Gene3Di1.10.287.110. 1 hit.
2.10.230.10. 1 hit.
HAMAPiMF_01152. DnaJ. 1 hit.
InterProiIPR012724. DnaJ.
IPR002939. DnaJ_C.
IPR001623. DnaJ_domain.
IPR018253. DnaJ_domain_CS.
IPR008971. HSP40/DnaJ_pept-bd.
IPR001305. HSP_DnaJ_Cys-rich_dom.
[Graphical view]
PfamiPF00226. DnaJ. 1 hit.
PF01556. DnaJ_C. 1 hit.
PF00684. DnaJ_CXXCXGXG. 1 hit.
[Graphical view]
PRINTSiPR00625. JDOMAIN.
SMARTiSM00271. DnaJ. 1 hit.
[Graphical view]
SUPFAMiSSF46565. SSF46565. 1 hit.
SSF49493. SSF49493. 3 hits.
SSF57938. SSF57938. 1 hit.
PROSITEiPS00636. DNAJ_1. 1 hit.
PS50076. DNAJ_2. 1 hit.
PS51188. ZF_CR. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDNJA3_MOUSE
AccessioniPrimary (citable) accession number: Q99M87
Secondary accession number(s): Q8BSM0
, Q99L09, Q99P71, Q99P76, Q9CT11, Q9DBJ7, Q9DC44
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 16, 2003
Last sequence update: June 1, 2001
Last modified: November 30, 2016
This is version 138 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.