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Protein

DnaJ homolog subfamily A member 3, mitochondrial

Gene

Dnaja3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Modulates apoptotic signal transduction or effector structures within the mitochondrial matrix. Affect cytochrome C release from the mitochondria and caspase 3 activation, but not caspase 8 activation. Isoform 1 increases apoptosis triggered by both TNF and the DNA-damaging agent mytomycin C; in sharp contrast, isoform 2 suppresses apoptosis. Can modulate IFN-gamma-mediated transcriptional activity (By similarity). Isoform 2 may play a role in neuromuscular junction development as an effector of the MUSK signaling pathway.By similarity1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi236Zinc 1By similarity1
Metal bindingi239Zinc 1By similarity1
Metal bindingi253Zinc 2By similarity1
Metal bindingi256Zinc 2By similarity1
Metal bindingi275Zinc 2By similarity1
Metal bindingi278Zinc 2By similarity1
Metal bindingi289Zinc 1By similarity1
Metal bindingi292Zinc 1By similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri223 – 301CR-typeAdd BLAST79

GO - Molecular functioni

  • ATP binding Source: InterPro
  • GTPase regulator activity Source: MGI
  • Hsp70 protein binding Source: Ensembl
  • interferon-gamma receptor binding Source: MGI
  • metal ion binding Source: UniProtKB-KW
  • NF-kappaB binding Source: MGI
  • protein kinase binding Source: MGI
  • receptor tyrosine kinase binding Source: UniProtKB
  • transcription factor binding Source: MGI
  • unfolded protein binding Source: MGI

GO - Biological processi

  • activation-induced cell death of T cells Source: MGI
  • activation of cysteine-type endopeptidase activity involved in apoptotic process Source: MGI
  • apoptotic process Source: MGI
  • cell aging Source: MGI
  • mitochondrial DNA replication Source: MGI
  • mitochondrion organization Source: MGI
  • negative regulation of apoptotic process Source: MGI
  • negative regulation of cell proliferation Source: MGI
  • negative regulation of cysteine-type endopeptidase activity involved in apoptotic process Source: MGI
  • negative regulation of I-kappaB kinase/NF-kappaB signaling Source: MGI
  • negative regulation of interferon-gamma-mediated signaling pathway Source: MGI
  • negative regulation of NF-kappaB transcription factor activity Source: MGI
  • negative regulation of programmed cell death Source: MGI
  • negative regulation of protein kinase activity Source: MGI
  • negative regulation of transcription from RNA polymerase II promoter Source: MGI
  • neuromuscular junction development Source: UniProtKB
  • positive regulation of apoptotic process Source: MGI
  • positive regulation of protein ubiquitination Source: MGI
  • positive regulation of T cell proliferation Source: MGI
  • protein folding Source: MGI
  • protein stabilization Source: MGI
  • response to heat Source: InterPro
  • response to interferon-gamma Source: MGI
  • skeletal muscle acetylcholine-gated channel clustering Source: UniProtKB
  • small GTPase mediated signal transduction Source: MGI
  • T cell differentiation in thymus Source: MGI

Keywordsi

Molecular functionChaperone
Biological processApoptosis
LigandMetal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
DnaJ homolog subfamily A member 3, mitochondrial
Alternative name(s):
DnaJ protein Tid-1
Short name:
mTid-1
Tumorous imaginal discs protein Tid56 homolog
Gene namesi
Name:Dnaja3
Synonyms:Tid1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 16

Organism-specific databases

MGIiMGI:1933786. Dnaja3.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell junction, Cell membrane, Cytoplasm, Membrane, Mitochondrion, Postsynaptic cell membrane, Synapse

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_0000007257? – 480DnaJ homolog subfamily A member 3, mitochondrial
Transit peptidei1 – ?MitochondrionSequence analysis

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei58Omega-N-methylarginine; by CARM1By similarity1
Modified residuei134N6-acetyllysineCombined sources1
Modified residuei238Omega-N-methylarginine; by CARM1By similarity1
Modified residuei293Omega-N-methylarginine; by CARM1By similarity1
Modified residuei398PhosphoserineBy similarity1

Post-translational modificationi

Tyrosine phosphorylated.

Keywords - PTMi

Acetylation, Methylation, Phosphoprotein

Proteomic databases

PaxDbiQ99M87.
PeptideAtlasiQ99M87.
PRIDEiQ99M87.

PTM databases

iPTMnetiQ99M87.
PhosphoSitePlusiQ99M87.

Expressioni

Gene expression databases

BgeeiENSMUSG00000004069.
CleanExiMM_DNAJA3.
ExpressionAtlasiQ99M87. baseline and differential.
GenevisibleiQ99M87. MM.

Interactioni

Subunit structurei

Interacts with JAK2, HSPA9B and IFN-gammaR2 chain. Interacts with Ras GTPase-activating protein 1 (RASA1). Isoform 2 interacts with MUSK (via the cytoplasmic domain).2 Publications

GO - Molecular functioni

Protein-protein interaction databases

BioGridi219986. 4 interactors.
IntActiQ99M87. 5 interactors.
MINTiMINT-1847235.
STRINGi10090.ENSMUSP00000053842.

Structurei

3D structure databases

ProteinModelPortaliQ99M87.
SMRiQ99M87.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini93 – 158JAdd BLAST66
Repeati236 – 243CXXCXGXG motif8
Repeati253 – 260CXXCXGXG motif8
Repeati275 – 282CXXCXGXG motif8
Repeati289 – 296CXXCXGXG motif8

Domaini

Modulation of apoptosis, i.e. proapoptotic activity of isoform 1 and antiapoptotic activity of isoform 2, is J domain-dependent (By similarity).By similarity

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri223 – 301CR-typeAdd BLAST79

Keywords - Domaini

Repeat, Transit peptide, Zinc-finger

Phylogenomic databases

eggNOGiKOG0715. Eukaryota.
COG0484. LUCA.
GeneTreeiENSGT00890000139473.
HOVERGENiHBG051371.
InParanoidiQ99M87.
KOiK09504.
OMAiDMGGFAD.
OrthoDBiEOG091G09C7.
PhylomeDBiQ99M87.
TreeFamiTF105152.

Family and domain databases

CDDicd06257. DnaJ. 1 hit.
cd10719. DnaJ_zf. 1 hit.
Gene3Di1.10.287.110. 1 hit.
HAMAPiMF_01152. DnaJ. 1 hit.
InterProiView protein in InterPro
IPR012724. DnaJ.
IPR002939. DnaJ_C.
IPR036869. DnaJ_dom_sf.
IPR001623. DnaJ_domain.
IPR018253. DnaJ_domain_CS.
IPR008971. HSP40/DnaJ_pept-bd.
IPR001305. HSP_DnaJ_Cys-rich_dom.
IPR036410. HSP_DnaJ_Cys-rich_dom_sf.
PfamiView protein in Pfam
PF00226. DnaJ. 1 hit.
PF01556. DnaJ_C. 1 hit.
PF00684. DnaJ_CXXCXGXG. 1 hit.
PRINTSiPR00625. JDOMAIN.
SMARTiView protein in SMART
SM00271. DnaJ. 1 hit.
SUPFAMiSSF46565. SSF46565. 1 hit.
SSF49493. SSF49493. 3 hits.
SSF57938. SSF57938. 1 hit.
PROSITEiView protein in PROSITE
PS00636. DNAJ_1. 1 hit.
PS50076. DNAJ_2. 1 hit.
PS51188. ZF_CR. 1 hit.

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q99M87-1) [UniParc]FASTAAdd to basket
Also known as: Tid-1L, TID1L

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAAWCSPRWL RVAVGTPRLP AAAGRGVQQP QGGVVATSLC RKLCVSAFGL
60 70 80 90 100
SMGAHGPRAL LTLRPGVRLT GTKSFPFVCT TSFHTSASLA KDDYYQILGV
110 120 130 140 150
PRNASQKDIK KAYYQLAKKY HPDTNKDDPK AKEKFSQLAE AYEVLSDEVK
160 170 180 190 200
RKQYDAYGSA GFDPGTSSSG QGYWRGGPSV DPEELFRKIF GEFSSSPFGD
210 220 230 240 250
FQNVFDQPQE YIMELTFNQA AKGVNKEFTV NIMDTCERCD GKGNEPGTKV
260 270 280 290 300
QHCHYCGGSG METINTGPFV MRSTCRRCGG RGSIITNPCV VCRGAGQAKQ
310 320 330 340 350
KKRVTIPVPA GVEDGQTVRM PVGKREIFVT FRVQKSPVFR RDGADIHSDL
360 370 380 390 400
FISIAQAILG GTAKAQGLYE TINVTIPAGI QTDQKIRLTG KGIPRINSYG
410 420 430 440 450
YGDHYIHIKI RVPKRLSSRQ QNLILSYAED ETDVEGTVNG VTHTSTGGRT
460 470 480
MDSSAGSKDR REAGEDNEGF LSKLKKIFTS
Length:480
Mass (Da):52,443
Last modified:June 1, 2001 - v1
Checksum:i30AA5557C18665A8
GO
Isoform 2 (identifier: Q99M87-2) [UniParc]FASTAAdd to basket
Also known as: Tid-1S, TID1S

The sequence of this isoform differs from the canonical sequence as follows:
     448-453: GRTMDS → KRSTGN
     454-480: Missing.

Show »
Length:453
Mass (Da):49,486
Checksum:iA5798CD5C8071EA7
GO
Isoform 3 (identifier: Q99M87-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     211-261: Missing.

Show »
Length:429
Mass (Da):46,847
Checksum:iF549A184D4EFD96F
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti403D → H in BAB23661 (PubMed:16141072).Curated1
Sequence conflicti456G → E in BAB23384 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_007440211 – 261Missing in isoform 3. 1 PublicationAdd BLAST51
Alternative sequenceiVSP_007427448 – 453GRTMDS → KRSTGN in isoform 2. 3 Publications6
Alternative sequenceiVSP_007428454 – 480Missing in isoform 2. 3 PublicationsAdd BLAST27

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY009320 mRNA. Translation: AAG37303.1.
AF325535 mRNA. Translation: AAK11222.1.
AF326358 mRNA. Translation: AAK11223.1.
AK004575 mRNA. Translation: BAB23384.1.
AK004910 mRNA. Translation: BAB23661.1.
AK011535 mRNA. Translation: BAB27682.2.
AK031250 mRNA. Translation: BAC27321.1.
BC003920 mRNA. Translation: AAH03920.1.
BC027240 mRNA. Translation: AAH27240.1.
CCDSiCCDS27922.1. [Q99M87-1]
CCDS49748.1. [Q99M87-2]
RefSeqiNP_001128584.1. NM_001135112.1. [Q99M87-2]
NP_076135.3. NM_023646.4. [Q99M87-1]
UniGeneiMm.248337.

Genome annotation databases

EnsembliENSMUST00000060067; ENSMUSP00000053842; ENSMUSG00000004069. [Q99M87-1]
ENSMUST00000115854; ENSMUSP00000111520; ENSMUSG00000004069. [Q99M87-2]
GeneIDi83945.
KEGGimmu:83945.
UCSCiuc007yac.2. mouse. [Q99M87-1]
uc012aat.1. mouse. [Q99M87-3]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiDNJA3_MOUSE
AccessioniPrimary (citable) accession number: Q99M87
Secondary accession number(s): Q8BSM0
, Q99L09, Q99P71, Q99P76, Q9CT11, Q9DBJ7, Q9DC44
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 16, 2003
Last sequence update: June 1, 2001
Last modified: October 25, 2017
This is version 144 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot