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Q99M80 (PTPRT_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 115. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Receptor-type tyrosine-protein phosphatase T

Short name=R-PTP-T
EC=3.1.3.48
Alternative name(s):
RPTPmam4
Receptor-type tyrosine-protein phosphatase rho
Short name=RPTP-rho
Short name=mRPTPrho
Gene names
Name:Ptprt
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length1454 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

May be involved in both signal transduction and cellular adhesion in the CNS. May have specific signaling roles in the tyrosine phosphorylation/dephosphorylation pathway in the anterior compartment of the adult cerebellar cortex.

Catalytic activity

Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.

Subcellular location

Membrane; Single-pass type I membrane protein.

Tissue specificity

Expression is restricted to the CNS. Distributed throughout the brain and spinal cord. Ref.5

Developmental stage

Exceptionally high levels found in the cortex and olfactory bulbs during perinatal development and are down-regulated during postnatal week 2. Expression in the cerebellar cortex is restricted to the granule cell layer of lobules 1-6. Anterior and posterior compartments become discernible only during postnatal weeks 2 and 6.

Sequence similarities

Belongs to the protein-tyrosine phosphatase family. Receptor class 2B subfamily.

Contains 4 fibronectin type-III domains.

Contains 1 Ig-like C2-type (immunoglobulin-like) domain.

Contains 1 MAM domain.

Contains 2 tyrosine-protein phosphatase domains.

Ontologies

Keywords
   Cellular componentMembrane
   Coding sequence diversityAlternative splicing
   DomainImmunoglobulin domain
Repeat
Signal
Transmembrane
Transmembrane helix
   Molecular functionHydrolase
Protein phosphatase
Receptor
   PTMDisulfide bond
Glycoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processhomophilic cell adhesion

Inferred from electronic annotation. Source: Ensembl

peptidyl-tyrosine dephosphorylation

Inferred from mutant phenotype PubMed 16973135. Source: GOC

protein dephosphorylation

Inferred from direct assay PubMed 16973135. Source: MGI

transmembrane receptor protein tyrosine kinase signaling pathway

Inferred from electronic annotation. Source: Ensembl

   Cellular_componentcell surface

Inferred from electronic annotation. Source: Ensembl

integral component of membrane

Inferred from electronic annotation. Source: UniProtKB-KW

plasma membrane

Traceable author statement. Source: Reactome

   Molecular_functionalpha-actinin binding

Inferred from direct assay PubMed 16973135. Source: MGI

alpha-catenin binding

Inferred from direct assay PubMed 16973135. Source: MGI

beta-catenin binding

Inferred from direct assay PubMed 16973135. Source: MGI

cadherin binding

Inferred from physical interaction PubMed 16973135. Source: MGI

delta-catenin binding

Inferred from physical interaction PubMed 16973135. Source: MGI

gamma-catenin binding

Inferred from physical interaction PubMed 16973135. Source: MGI

protein tyrosine phosphatase activity

Inferred from mutant phenotype PubMed 16973135. Source: MGI

thiolester hydrolase activity

Inferred from direct assay PubMed 16973135. Source: MGI

Complete GO annotation...

Alternative products

This entry describes 5 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q99M80-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q99M80-2)

The sequence of this isoform differs from the canonical sequence as follows:
     731-749: Missing.
     1007-1007: R → RHPAEHTVGTATLGRAASPGM
Isoform 3 (identifier: Q99M80-3)

Also known as: RPTPrho2;

The sequence of this isoform differs from the canonical sequence as follows:
     731-749: Missing.
     794-794: R → RRNAYSYSYYL
Isoform 4 (identifier: Q99M80-4)

Also known as: RPTPrho1;

The sequence of this isoform differs from the canonical sequence as follows:
     731-749: Missing.
Isoform 5 (identifier: Q99M80-5)

The sequence of this isoform differs from the canonical sequence as follows:
     731-749: Missing.
     794-794: R → RRNAYSYSYYLSQR

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2929 Potential
Chain30 – 14541425Receptor-type tyrosine-protein phosphatase T
PRO_0000025464

Regions

Topological domain30 – 770741Extracellular Potential
Transmembrane771 – 79121Helical; Potential
Topological domain792 – 1454663Cytoplasmic Potential
Domain34 – 195162MAM
Domain197 – 28892Ig-like C2-type
Domain295 – 38894Fibronectin type-III 1
Domain393 – 48795Fibronectin type-III 2
Domain488 – 594107Fibronectin type-III 3
Domain670 – 76798Fibronectin type-III 4
Domain902 – 1156255Tyrosine-protein phosphatase 1
Domain1188 – 1450263Tyrosine-protein phosphatase 2
Region1097 – 11037Substrate binding By similarity

Sites

Active site10971Phosphocysteine intermediate By similarity
Active site13911Phosphocysteine intermediate By similarity
Binding site10651Substrate By similarity
Binding site11411Substrate By similarity

Amino acid modifications

Glycosylation821N-linked (GlcNAc...) Potential
Glycosylation1021N-linked (GlcNAc...) Potential
Glycosylation1411N-linked (GlcNAc...) Potential
Glycosylation2121N-linked (GlcNAc...) Potential
Glycosylation4251N-linked (GlcNAc...) Potential
Glycosylation5141N-linked (GlcNAc...) Potential
Glycosylation5511N-linked (GlcNAc...) Potential
Glycosylation6051N-linked (GlcNAc...) Potential
Glycosylation6581N-linked (GlcNAc...) Potential
Glycosylation6881N-linked (GlcNAc...) Potential
Disulfide bond217 ↔ 271 Potential

Natural variations

Alternative sequence731 – 74919Missing in isoform 2, isoform 3, isoform 4 and isoform 5.
VSP_007803
Alternative sequence7941R → RRNAYSYSYYL in isoform 3.
VSP_007804
Alternative sequence7941R → RRNAYSYSYYLSQR in isoform 5.
VSP_007805
Alternative sequence10071R → RHPAEHTVGTATLGRAASPG M in isoform 2.
VSP_007806

Experimental info

Sequence conflict13 – 164Missing in AAD34158. Ref.1
Sequence conflict211R → P in AAD34158. Ref.1
Sequence conflict34 – 374GGCS → RGVF in AAD34158. Ref.1
Sequence conflict871A → T in AAF82401. Ref.4
Sequence conflict2541A → S in AAF82401. Ref.4
Sequence conflict2661I → V in AAF82401. Ref.4
Sequence conflict6021T → S in AAF82401. Ref.4
Sequence conflict8221A → T in AAF82401. Ref.4
Sequence conflict8251G → S in AAF82401. Ref.4
Sequence conflict844 – 8452TD → N in AAF82401. Ref.4
Sequence conflict10161D → A in AAF82401. Ref.4
Sequence conflict10491Y → H in AAD34158. Ref.1
Sequence conflict10501H → N in AAF82401. Ref.4
Sequence conflict10761L → V in AAF82401. Ref.4
Sequence conflict11031R → K in AAD34158. Ref.1
Sequence conflict12591F → L in AAF82401. Ref.4
Sequence conflict12661L → I in AAF82401. Ref.4
Sequence conflict12691T → S in AAF82401. Ref.4

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified July 19, 2003. Version 2.
Checksum: C60464F7B423F8A8

FASTA1,454163,012
        10         20         30         40         50         60 
MGSLGGLALC LLRLLLLGLQ RPPLPGAGAQ SAAGGCSFDE HYSNCGYSVA LGTNGFTWEQ 

        70         80         90        100        110        120 
INTWEKPMLD PAVPTGSFMM VNSSGRASGQ KAHLLLPTLK ENDTHCIDFH YYFSSRDRSS 

       130        140        150        160        170        180 
PGALNVYVKV NGGPQGNPVW NVSGVVTEGW VKAELAISTF WPHFYQVIFE SVSLKGHPGY 

       190        200        210        220        230        240 
IAVDEVRVLA HPCRKAPHFL RLQNVEVNVG QNATFQCIAG GKWSQHDKLW LQQWNGRDTA 

       250        260        270        280        290        300 
LMVTRVVNHR RFSATVSVAD TSQRSISKYR CVIRSDGGSG VSNYAELIVK EPPTPIAPPE 

       310        320        330        340        350        360 
LLAVGATYLW IKPNANSIIG DGPIILKEVE YRTTTGTWAE THIVDSPNYK LWHLDPDVEY 

       370        380        390        400        410        420 
EIRVLLTRPG EGGTGPPGPP LTTRTKCADP VHGPQNVEIV DIRARQLTLQ WEPFGYAVTR 

       430        440        450        460        470        480 
CHSYNLTVQY QYVFNQQQYE AEEVIQTSSH YTLRGLRPFM TIRLRLLLSN PEGRMESEEL 

       490        500        510        520        530        540 
VVQTEEDVPG AVPLESIQGG PFEEKIYIQW KPPNETNGVI TLYEINYKAV GSLDPSADLS 

       550        560        570        580        590        600 
SQRGKVFKLR NETHHLFVGL YPGTTYSFTI KASTAKGFGP PVTTRIATKI SAPSMPEYDA 

       610        620        630        640        650        660 
DTPLNETDTT ITVMLKPAQS RGAPVSVYQL VVKEERLQKS RRAADIIECF SVPVSYRNAS 

       670        680        690        700        710        720 
NLDSLHYFAA ELKPSNLPVT QPFTVGDNKT YNGYWNPPLS PLKSYSIYFQ ALSKANGETK 

       730        740        750        760        770        780 
INCVRLATKG APMGSAQVTP GTPLCLLTTA STQNSNTVEP EKQVDNTVKM AGVIAGLLMF 

       790        800        810        820        830        840 
IIILLGVMLT IKRRKLAKKQ KETQSGAQRE MGPVASTDKP TAKLGTNRND EGFSSSSQDV 

       850        860        870        880        890        900 
NGFTDGSRGE LSQPTLTIQT HPYRTCDPVE MSYPRDQFQP AIRVADLLQH ITQMKRGQGY 

       910        920        930        940        950        960 
GFKEEYEALP EGQTASWDTA KEDENRNKNR YGNIISYDHS RVRLLVLDGD PHSDYINANY 

       970        980        990       1000       1010       1020 
IDGYHRPRHY IATQGPMQET VKDFWRMIWQ ENSASIVMVT NLVEVGRVKC VRYWPDDTEV 

      1030       1040       1050       1060       1070       1080 
YGDIKVTLIE TEPLAEYVIR TFTVQKKGYH EIRELRLFHF TSWPDHGVPC YATGLLGFVR 

      1090       1100       1110       1120       1130       1140 
QVKFLNPPEA GPIVVHCSAG AGRTGCFIAI DTMLDMAENE GVVDIFNCVR ELRAQRVNLV 

      1150       1160       1170       1180       1190       1200 
QTEEQYVFVH DAILEACLCG NTAIPVCEFR SLYYNISRLD PQTNSSQIKD EFQTLNIVTP 

      1210       1220       1230       1240       1250       1260 
RVRPEDCSIG LLPRNHDKNR SMDVLPLDRC LPFLISVDGE SSNYINAALM DSHKQPAAFV 

      1270       1280       1290       1300       1310       1320 
VTQHPLPNTV ADFWRLVFDY NCSSVVMLNE MDTAQLCMQY WPEKTSGCYG PIQVEFVSAD 

      1330       1340       1350       1360       1370       1380 
IDEDIIHRIF RICNMARPQD GYRIVQHLQY IGWPAYRDTP PSKRSLLKVV RRLEKWQEQY 

      1390       1400       1410       1420       1430       1440 
DGREGRTVVH CLNGGGRSGT FCAICSVCEM IQQQNIIDVF HIVKTLRNNK SNMVETLEQY 

      1450 
KFVYEVALEY LSSF 

« Hide

Isoform 2 [UniParc].

Checksum: 95293974FAEA85A0
Show »

FASTA1,455163,115
Isoform 3 (RPTPrho2) [UniParc].

Checksum: 1879E76330FE2329
Show »

FASTA1,445162,453
Isoform 4 (RPTPrho1) [UniParc].

Checksum: 37E558F82049F50C
Show »

FASTA1,435161,172
Isoform 5 [UniParc].

Checksum: AF638DD3EA12DC5A
Show »

FASTA1,448162,825

References

[1]"Genomic organization and alternative splicing of the human and mouse RPTPrho genes."
Besco J.A., Frostholm A., Popesco M.C., Burghes A.H.M., Rotter A.
BMC Genomics 2:1-1(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 2 AND 5), ALTERNATIVE SPLICING.
Strain: C57BL/6J.
[2]Erratum
Besco J.A., Frostholm A., Popesco M.C., Burghes A.H.M., Rotter A.
BMC Genomics 2:5-5(2001) [PubMed] [Europe PMC] [Abstract]
[3]"RPTPmam4: a fourth member of the MAM family of receptor protein tyrosine phosphatases expressed in adult brain."
Buchli A.D., Zimmermann D.R., Pfister F., Vaughan L.
Submitted (MAR-2000) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1; 2; 3 AND 4).
[4]"Molecular cloning and functional characterization on mouse receptor-like protein tyrosine phosphatase, mRPTPrho, which mediates cell-cell adhesion of pancreatic beta cells."
Mizuta M., Bergman B., Miki T., Hutton J.C.
Submitted (JUL-2001) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 3 AND 4).
[5]"Novel receptor protein tyrosine phosphatase (RPTPrho) and acidic fibroblast growth factor (FGF-1) transcripts delineate a rostrocaudal boundary in the granule cell layer of the murine cerebellar cortex."
McAndrew P.E., Frostholm A., Evans J.E., Zdilar D., Goldowitz D., Chiu I.-M., Burghes A.H.M., Rotter A.
J. Comp. Neurol. 391:444-455(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF152556 mRNA. Translation: AAD34158.4.
AY026861 mRNA. Translation: AAK18741.1.
AY026862 mRNA. Translation: AAK18742.1.
AY026863 mRNA. Translation: AAK18743.1.
AF244125 mRNA. Translation: AAF44712.1.
AF162856 mRNA. Translation: AAF82400.2.
AF162857 mRNA. Translation: AAF82401.1.
RefSeqNP_067439.1. NM_021464.4.
XP_006499070.1. XM_006499007.1.
XP_006499071.1. XM_006499008.1.
UniGeneMm.235807.
Mm.381206.

3D structure databases

ProteinModelPortalQ99M80.
SMRQ99M80. Positions 33-595, 879-1454.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10090.ENSMUSP00000105067.

PTM databases

PhosphoSiteQ99M80.

Proteomic databases

PaxDbQ99M80.
PRIDEQ99M80.

Protocols and materials databases

DNASU19281.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000109441; ENSMUSP00000105067; ENSMUSG00000053141. [Q99M80-2]
ENSMUST00000109443; ENSMUSP00000105069; ENSMUSG00000053141. [Q99M80-3]
ENSMUST00000109445; ENSMUSP00000105071; ENSMUSG00000053141. [Q99M80-4]
GeneID19281.
KEGGmmu:19281.

Organism-specific databases

CTD11122.
MGIMGI:1321152. Ptprt.

Phylogenomic databases

eggNOGCOG5599.
GeneTreeENSGT00640000091300.
HOVERGENHBG062785.
KOK13297.
OMATHPYRTC.
OrthoDBEOG70KGNP.
TreeFamTF312900.

Enzyme and pathway databases

ReactomeREACT_188576. Developmental Biology.

Gene expression databases

ArrayExpressQ99M80.
BgeeQ99M80.
CleanExMM_PTPRT.
GenevestigatorQ99M80.

Family and domain databases

Gene3D2.60.40.10. 4 hits.
InterProIPR008985. ConA-like_lec_gl_sf.
IPR003961. Fibronectin_type3.
IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003599. Ig_sub.
IPR000998. MAM_dom.
IPR000387. Tyr/Dual-sp_Pase.
IPR016130. Tyr_Pase_AS.
IPR000242. Tyr_Pase_rcpt/non-rcpt.
[Graphical view]
PfamPF00041. fn3. 2 hits.
PF00629. MAM. 1 hit.
PF00102. Y_phosphatase. 2 hits.
[Graphical view]
PRINTSPR00020. MAMDOMAIN.
PR00700. PRTYPHPHTASE.
SMARTSM00060. FN3. 3 hits.
SM00409. IG. 1 hit.
SM00137. MAM. 1 hit.
SM00194. PTPc. 2 hits.
[Graphical view]
SUPFAMSSF49265. SSF49265. 2 hits.
SSF49899. SSF49899. 1 hit.
PROSITEPS50853. FN3. 3 hits.
PS50835. IG_LIKE. 1 hit.
PS00740. MAM_1. 1 hit.
PS50060. MAM_2. 1 hit.
PS00383. TYR_PHOSPHATASE_1. 2 hits.
PS50056. TYR_PHOSPHATASE_2. 2 hits.
PS50055. TYR_PHOSPHATASE_PTP. 2 hits.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSPTPRT. mouse.
NextBio296196.
PROQ99M80.
SOURCESearch...

Entry information

Entry namePTPRT_MOUSE
AccessionPrimary (citable) accession number: Q99M80
Secondary accession number(s): Q99M81 expand/collapse secondary AC list , Q99M82, Q9JIZ1, Q9JIZ2, Q9JKC2, Q9JLP0
Entry history
Integrated into UniProtKB/Swiss-Prot: July 19, 2003
Last sequence update: July 19, 2003
Last modified: April 16, 2014
This is version 115 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot