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Q99M64 (P4K2A_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified November 16, 2011. Version 55. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Phosphatidylinositol 4-kinase type 2-alpha
EC=2.7.1.67
Alternative name(s):
55 kDa type II phosphatidylinositol 4-kinase
Phosphatidylinositol 4-kinase type II-alpha
Gene names
Name:Pi4k2a
Synonyms:Pi4kii
OrganismRattus norvegicus (Rat)
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length478 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Together with PI4K2B and the type III PI4Ks (PIK4CA and PIK4CB) it contributes to the overall PI4-kinase activity of the cell. The phosphorylation of phosphatidylinositol (PI) to PI4P is the first committed step in the generation of phosphatidylinositol 4,5-bisphosphate (PIP2), a precursor of the second messenger inositol 1,4,5-trisphosphate (InsP3). Contributes to the production of InsP3 in stimulated cells. Ref.1

Catalytic activity

ATP + 1-phosphatidyl-1D-myo-inositol = ADP + 1-phosphatidyl-1D-myo-inositol 4-phosphate.

Subcellular location

Cytoplasm By similarity. Membrane By similarity. Membrane raft By similarity. Note: Found in subdomains of the plasma membrane termed non-caveolar membrane rafts By similarity.

Sequence similarities

Belongs to the PI3/PI4-kinase family. Type II PI4K subfamily.

Contains 1 PI3K/PI4K domain.

Biophysicochemical properties

Kinetic parameters:

KM=88 µM for ATP

KM=45 µM for PtdIns

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 478478Phosphatidylinositol 4-kinase type 2-alpha
PRO_0000285160

Regions

Domain132 – 437306PI3K/PI4K

Amino acid modifications

Modified residue51Phosphoserine By similarity
Modified residue91Phosphoserine By similarity
Modified residue431Phosphoserine By similarity
Modified residue461Phosphoserine By similarity
Modified residue501Phosphoserine By similarity
Modified residue4601Phosphoserine By similarity
Modified residue4611Phosphoserine By similarity
Modified residue4631Phosphoserine By similarity

Sequences

Sequence LengthMass (Da)Tools
Q99M64 [UniParc].

Last modified June 1, 2001. Version 1.
Checksum: EBE4BFFB4574FD1F

FASTA47854,305
        10         20         30         40         50         60 
MDETSPLVSP ERAQPPEYTF PSVSGAHFPQ VPGGAVRVAA AGSGPSPPCS PGHDRERQPL 

        70         80         90        100        110        120 
LDRARGAAAQ GQTHTVAAQA QALAAQAAVA VHAVQTHRER NDFPEDPEFE VVVRQAEIAI 

       130        140        150        160        170        180 
ECSIYPERIY QGSSGSYFVK DSQGRIIAVF KPKNEEPYGN LNPKWTKWLQ KLCCPCCFGR 

       190        200        210        220        230        240 
DCLVLNQGYL SEAGASLVDQ KLELNIVPRT KVVYLASETF NYSAIDRVKS RGKRLALEKV 

       250        260        270        280        290        300 
PKVGQRFNRI GLPPKVGSFQ LFVEGYKDAD YWLRRFEAEP LPENTNRQLL LQFERLVVLD 

       310        320        330        340        350        360 
YIIRNTDRGN DNWLIKYDYP MDNPNCRDTD WVMVREPVIK VAAIDNGLAF PLKHPDSWRA 

       370        380        390        400        410        420 
YPFYWAWLPQ AKVPFSQEIK DLILPKISDP NFVKDLEEDL YELFKKDPGF DRGQFHKQIA 

       430        440        450        460        470 
VMRGQILNLT QALKDNKSPL HLVQMPPVIV ETARSHQRSS SESYTQSFQS RKPFFSWW

« Hide

References

[1]"A novel family of phosphatidylinositol 4-kinases conserved from yeast to humans."
Barylko B., Gerber S.H., Binns D.D., Grichine N., Khvotchev M., Suedhof T.C., Albanesi J.P.
J. Biol. Chem. 276:7705-7708(2001) [PubMed: 11244087] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION.
Tissue: Brain.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AY029199 mRNA. Translation: AAK33002.1.
IPIIPI00197470.
RefSeqNP_446187.1. NM_053735.1.
UniGeneRn.162533.

3D structure databases

ModBaseSearch...

Protein-protein interaction databases

STRINGQ99M64.

PTM databases

PhosphoSiteQ99M64.

Proteomic databases

PRIDEQ99M64.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000019874; ENSRNOP00000019874; ENSRNOG00000014675.
GeneID114554.
KEGGrno:114554.
UCSCNM_053735. rat.

Organism-specific databases

CTD55361.
RGD620485. Pi4k2a.

Phylogenomic databases

eggNOGroNOG05931.
GeneTreeENSGT00390000010434.
HOVERGENHBG059542.
InParanoidQ99M64.
OMAQRFHRIG.
OrthoDBEOG4VT5X4.
PhylomeDBQ99M64.

Gene expression databases

ArrayExpressQ99M64.
GenevestigatorQ99M64.

Family and domain databases

InterProIPR000403. PI3/4_kinase_cat.
[Graphical view]
KOK13711.
PfamPF00454. PI3_PI4_kinase. 1 hit.
[Graphical view]
PROSITEPS00915. PI3_4_KINASE_1. False negative.
PS00916. PI3_4_KINASE_2. False negative.
PS50290. PI3_4_KINASE_3. False negative.
[Graphical view]
ProtoNetSearch...

Other

NextBio618693.

Entry information

Entry nameP4K2A_RAT
AccessionPrimary (citable) accession number: Q99M64
Entry history
Integrated into UniProtKB/Swiss-Prot: May 1, 2007
Last sequence update: June 1, 2001
Last modified: November 16, 2011
This is version 55 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

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