Reviewed, UniProtKB/Swiss-Prot Q99M64 (P4K2A_RAT)

Names and origin

Protein names

Recommended name:
    Phosphatidylinositol 4-kinase type 2-alpha
    EC=2.7.1.67
Alternative name(s):
    Phosphatidylinositol 4-kinase type II-alpha
    55 kDa type II phosphatidylinositol 4-kinase

Gene names

Name:	Pi4k2a
Synonyms:	Pi4kii

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	478 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Together with PI4K2B and the type III PI4Ks (PIK4CA and PIK4CB) it contributes to the overall PI4-kinase activity of the cell. The phosphorylation of phosphatidylinositol (PI) to PI4P is the first committed step in the generation of phosphatidylinositol 4,5-bisphosphate (PIP2), a precursor of the second messenger inositol 1,4,5-trisphosphate (InsP3). Contributes to the production of InsP3 in stimulated cells. Ref.1
Catalytic activity	ATP + 1-phosphatidyl-1D-myo-inositol = ADP + 1-phosphatidyl-1D-myo-inositol 4-phosphate.
Subcellular location	Cytoplasm By similarity. Membrane By similarity. Membrane raft By similarity. Note: Found in subdomains of the plasma membrane termed non-caveolar membrane rafts By similarity.
Sequence similarities	Belongs to the PI3/PI4-kinase family. Type II PI4K subfamily. Contains 1 PI3K/PI4K domain.
Biophysicochemical properties	Kinetic parameters: KM=88 µM for ATP KM=45 µM for PtdIns

Ontologies

Keywords
Cellular component	Cytoplasm Membrane
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	basophil degranulation Inferred from mutant phenotype. Source: RGD
Cellular component	early endosome membrane Inferred from direct assay. Source: RGD exocytic vesicle Inferred from direct assay. Source: RGD membrane raft Inferred from direct assay. Source: RGD microsome Inferred from direct assay. Source: RGD perikaryon Inferred from direct assay. Source: RGD protein complex Inferred from direct assay. Source: RGD synaptic vesicle membrane Inferred from direct assay. Source: RGD
Molecular function	1-phosphatidylinositol 4-kinase activity Inferred from direct assay. Source: RGD ATP binding Inferred from electronic annotation. Source: UniProtKB-KW protein complex binding Inferred from direct assay. Source: RGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 478	478	Phosphatidylinositol 4-kinase type 2-alpha		PRO_0000285160
Regions
	Domain	132 – 437	306	PI3K/PI4K
Amino acid modifications
	Modified residue	5	1	Phosphoserine By similarity
	Modified residue	9	1	Phosphoserine By similarity
	Modified residue	43	1	Phosphoserine By similarity
	Modified residue	46	1	Phosphoserine By similarity
	Modified residue	50	1	Phosphoserine By similarity
	Modified residue	460	1	Phosphoserine By similarity
	Modified residue	461	1	Phosphoserine By similarity
	Modified residue	463	1	Phosphoserine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q99M64-1 [UniParc]. Last modified June 1, 2001. Version 1. Checksum: EBE4BFFB4574FD1F	FASTA	478	54,305
	10 20 30 40 50 60 MDETSPLVSP ERAQPPEYTF PSVSGAHFPQ VPGGAVRVAA AGSGPSPPCS PGHDRERQPL 70 80 90 100 110 120 LDRARGAAAQ GQTHTVAAQA QALAAQAAVA VHAVQTHRER NDFPEDPEFE VVVRQAEIAI 130 140 150 160 170 180 ECSIYPERIY QGSSGSYFVK DSQGRIIAVF KPKNEEPYGN LNPKWTKWLQ KLCCPCCFGR 190 200 210 220 230 240 DCLVLNQGYL SEAGASLVDQ KLELNIVPRT KVVYLASETF NYSAIDRVKS RGKRLALEKV 250 260 270 280 290 300 PKVGQRFNRI GLPPKVGSFQ LFVEGYKDAD YWLRRFEAEP LPENTNRQLL LQFERLVVLD 310 320 330 340 350 360 YIIRNTDRGN DNWLIKYDYP MDNPNCRDTD WVMVREPVIK VAAIDNGLAF PLKHPDSWRA 370 380 390 400 410 420 YPFYWAWLPQ AKVPFSQEIK DLILPKISDP NFVKDLEEDL YELFKKDPGF DRGQFHKQIA 430 440 450 460 470 VMRGQILNLT QALKDNKSPL HLVQMPPVIV ETARSHQRSS SESYTQSFQS RKPFFSWW

References

[1]	"A novel family of phosphatidylinositol 4-kinases conserved from yeast to humans." Barylko B., Gerber S.H., Binns D.D., Grichine N., Khvotchev M., Suedhof T.C., Albanesi J.P. J. Biol. Chem. 276:7705-7708(2001) [PubMed: 11244087] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION. Tissue: Brain.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY029199 mRNA. Translation: AAK33002.1.
IPI	IPI00197470.
RefSeq	NP_446187.1.
UniGene	Rn.162533
3D structure databases
ModBase	Search...
PTM databases
PhosphoSite	Q99M64.
Proteomic databases
PRIDE	Q99M64.
Genome annotation databases
Ensembl	ENSRNOT00000019874; ENSRNOP00000019874; ENSRNOG00000014675; Rattus norvegicus. [Genome view]
GeneID	114554.
KEGG	rno:114554.
UCSC	NM_053735. rat.
Organism-specific databases
CTD	114554.
RGD	620485. Pi4k2a.
Phylogenomic databases
eggNOG	roNOG05931.
HOVERGEN	Q99M64.
InParanoid	Q99M64.
OMA	RAQPPEY.
OrthoDB	EOG9NW24V.
PhylomeDB	Q99M64.
Enzyme and pathway databases
BRENDA	2.7.1.67. 248.
Gene expression databases
ArrayExpress	Q99M64.
Genevestigator	Q99M64.
Family and domain databases
InterPro	IPR000403. PI3/4_kinase_cat. [Graphical view]
Pfam	PF00454. PI3_PI4_kinase. 1 hit. [Graphical view]
PROSITE	PS00915. PI3_4_KINASE_1. False negative. PS00916. PI3_4_KINASE_2. False negative. PS50290. PI3_4_KINASE_3. False negative. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	618693.

Entry information

Entry name

P4K2A_RAT

Accession

Primary (citable) accession number: Q99M64

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 2007
Last sequence update:	June 1, 2001
Last modified:	January 19, 2010
This is version 43 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)