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Protein

WD40 repeat-containing protein SMU1

Gene

Smu1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Auxiliary spliceosomal protein that regulates selection of alternative splice sites in a small set of target pre-mRNA species. Regulates alternative splicing of the HSPG2 pre-mRNA. Required for normal mitotic spindle assembly and normal progress through mitosis.By similarity

GO - Biological processi

Keywordsi

Biological processmRNA processing, mRNA splicing

Names & Taxonomyi

Protein namesi
Recommended name:
WD40 repeat-containing protein SMU1
Alternative name(s):
Brain-enriched WD repeat-containing protein
Smu-1 suppressor of mec-8 and unc-52 protein homolog
Cleaved into the following chain:
Gene namesi
Name:Smu1
Synonyms:Bwd
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi620694. Smu1.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus, Spliceosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004245221 – 513WD40 repeat-containing protein SMU1Add BLAST513
Initiator methionineiRemoved; alternateBy similarity
ChainiPRO_00002375922 – 513WD40 repeat-containing protein SMU1, N-terminally processedAdd BLAST512

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Modified residuei2N-acetylserine; in WD40 repeat-containing protein SMU1, N-terminally processedBy similarity1
Cross-linki379Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity

Keywords - PTMi

Acetylation, Isopeptide bond, Ubl conjugation

Proteomic databases

PaxDbiQ99M63.
PRIDEiQ99M63.

Expressioni

Tissue specificityi

Highly expressed in cerebellum, midbrain and heart. Moderately expressed in brain stem, cerebral cortex and liver. Detected in dentate gyrus, pyramidal layer of the hippocampus and Purkinje and granule neurons of cerebellum.1 Publication

Interactioni

Subunit structurei

Interacts with IK. Probable component of the spliceosome B complex.By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000010463.

Structurei

3D structure databases

ProteinModelPortaliQ99M63.
SMRiQ99M63.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini6 – 38LisHPROSITE-ProRule annotationAdd BLAST33
Domaini40 – 92CTLHPROSITE-ProRule annotationAdd BLAST53
Repeati212 – 253WD 1Sequence analysisAdd BLAST42
Repeati262 – 303WD 2Sequence analysisAdd BLAST42
Repeati305 – 346WD 3Sequence analysisAdd BLAST42
Repeati347 – 386WD 4Sequence analysisAdd BLAST40
Repeati395 – 436WD 5Sequence analysisAdd BLAST42
Repeati440 – 479WD 6Sequence analysisAdd BLAST40
Repeati482 – 513WD 7Sequence analysisAdd BLAST32

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 315Required for interaction with IKBy similarityAdd BLAST315

Domaini

The WD repeats assemble into a seven-bladed WD propeller.By similarity

Sequence similaritiesi

Belongs to the WD repeat SMU1 family.Curated

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

eggNOGiKOG0275. Eukaryota.
ENOG410XP39. LUCA.
HOGENOMiHOG000264846.
HOVERGENiHBG079573.
InParanoidiQ99M63.
KOiK13111.
PhylomeDBiQ99M63.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiView protein in InterPro
IPR006595. CTLH_C.
IPR020472. G-protein_beta_WD-40_rep.
IPR006594. LisH.
IPR015943. WD40/YVTN_repeat-like_dom_sf.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
IPR036322. WD40_repeat_dom_sf.
PfamiView protein in Pfam
PF00400. WD40. 5 hits.
PRINTSiPR00320. GPROTEINBRPT.
SMARTiView protein in SMART
SM00668. CTLH. 1 hit.
SM00667. LisH. 1 hit.
SM00320. WD40. 7 hits.
SUPFAMiSSF50978. SSF50978. 1 hit.
PROSITEiView protein in PROSITE
PS50897. CTLH. 1 hit.
PS50896. LISH. 1 hit.
PS00678. WD_REPEATS_1. 2 hits.
PS50082. WD_REPEATS_2. 5 hits.
PS50294. WD_REPEATS_REGION. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q99M63-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSIEIESSDV IRLIMQYLKE NSLHRALATF TEETTVSLNT VDSIESFVAD
60 70 80 90 100
INSGHWDTVL QAIQSLKLPD KTLIDLYEQV VLELIELREL GAARSLLRQT
110 120 130 140 150
DPMIMLKQTQ PERYIHLENL LARSYFDPRE AYPDGSSKEK RRAAIAQALA
160 170 180 190 200
GEVSVVPPSR LMALLGQALK WQQHQGLLPP GMTIDLFRGK AAVKDVEEEK
210 220 230 240 250
FPTQLSRHIK FGQKSHVECA RFSPDGQYLV TGSVDGFIEV WNFTTGKIRK
260 270 280 290 300
DLKYQAQDNF MMMDDAVLCM CFSRDTEMLA TGAQDGKIKV WKIQSGQCLR
310 320 330 340 350
RFERAHSKGV TCLSFSKDSS QILSASFDQT IRIHGLKSGK TLKEFRGHSS
360 370 380 390 400
FVNEATFTQD GHYIISASSD GTVKIWNMKT TECSNTFKSL GSTAGTDITV
410 420 430 440 450
NSVILLPKNP EHFVVCNRSN TVVIMNMQGQ IVRSFSSGKR EGGDFVCCAL
460 470 480 490 500
SPRGEWIYCV GEDFVLYCFS TVTGKLERTL TVHEKDVIGI AHHPHQNLIA
510
TYSEDGLLKL WKP
Length:513
Mass (Da):57,551
Last modified:June 1, 2001 - v1
Checksum:i9BE313C1AFA1875A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY029526 mRNA. Translation: AAK33013.1.
RefSeqiNP_476543.1. NM_057195.1.
UniGeneiRn.54978.

Genome annotation databases

GeneIDi117541.
KEGGirno:117541.
UCSCiRGD:620694. rat.

Similar proteinsi

Entry informationi

Entry nameiSMU1_RAT
AccessioniPrimary (citable) accession number: Q99M63
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2006
Last sequence update: June 1, 2001
Last modified: November 22, 2017
This is version 105 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families