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Q99M54 (CDCA3_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 76. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Cell division cycle-associated protein 3
Alternative name(s):
Gene-rich cluster protein C8
Trigger of mitotic entry protein 1
Short name=TOME-1
Gene names
Name:Cdca3
Synonyms:C8, Grcc8, Tome1
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length266 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

F-box-like protein which is required for entry into mitosis. Acts by participating in E3 ligase complexes that mediate the ubiquitination and degradation of WEE1 kinase at G2/M phase By similarity.

Pathway

Protein modification; protein ubiquitination.

Subunit structure

Interacts with SKP1. Part of a SCF (SKP1-cullin-F-box) protein ligase complex. Ref.5

Subcellular location

Cytoplasmcytosol By similarity.

Domain

The KEN box is required for the association with the APC/C-Cdh1 complex By similarity.

Post-translational modification

Ubiquitinated and degraded by the APC/C-Cdh1 complex By similarity.

Sequence caution

The sequence AAH27172.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
   Biological processCell cycle
Cell division
Mitosis
Ubl conjugation pathway
   Cellular componentCytoplasm
   Coding sequence diversityAlternative splicing
   PTMPhosphoprotein
Ubl conjugation
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processmitotic nuclear division

Inferred from electronic annotation. Source: UniProtKB-KW

protein ubiquitination

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Cellular_componentcytosol

Inferred from electronic annotation. Source: UniProtKB-SubCell

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q99M54-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q99M54-2)

The sequence of this isoform differs from the canonical sequence as follows:
     83-84: DP → GK
     85-266: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 266266Cell division cycle-associated protein 3
PRO_0000287709

Regions

Region90 – 11930F-box-like
Motif256 – 2583KEN box

Amino acid modifications

Modified residue291Phosphoserine Ref.6
Modified residue311Phosphoserine By similarity
Modified residue371Phosphothreonine By similarity
Modified residue441Phosphoserine By similarity
Modified residue671Phosphoserine Ref.6
Modified residue751Phosphothreonine By similarity
Modified residue931Phosphoserine By similarity
Modified residue1971Phosphoserine By similarity
Modified residue2001Phosphothreonine By similarity
Modified residue2071Phosphoserine By similarity

Natural variations

Alternative sequence83 – 842DP → GK in isoform 2.
VSP_025606
Alternative sequence85 – 266182Missing in isoform 2.
VSP_025607

Experimental info

Sequence conflict1191Y → S in AAH02006. Ref.4
Sequence conflict1261D → G in AAH02006. Ref.4

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified May 15, 2007. Version 2.
Checksum: 8E02E77ACAAAC2F8

FASTA26628,708
        10         20         30         40         50         60 
MGSTQSVSGT PARPLPRNKQ VARVADPRSP SAGIQRTPIQ VESSPQPSLP AEQLNGLKQA 

        70         80         90        100        110        120 
QDPDPRSPTL GIARTPMKIS GPDPQCSLVK ELSEVLETEA SESISSPELA LPRETPLFYD 

       130        140        150        160        170        180 
LDLSSDPQLS PEDQLLPWSQ AELDPKQVFT KEEAKQSAET IAASQNSDKP SRDPETPQSS 

       190        200        210        220        230        240 
GSKRSRRKAN SKVLGRSPLT ILQDDNSPGT LTLRQGKRPS ALSENVKDLK EGVVLGTGRF 

       250        260 
LKAGGGAREP NQDHDKENQH FALLES 

« Hide

Isoform 2 [UniParc].

Checksum: 4D3621305F809E22
Show »

FASTA848,791

References

« Hide 'large scale' references
[1]"Comparative sequence analysis of a gene-rich cluster at human chromosome 12p13 and its syntenic region in mouse chromosome 6."
Ansari-Lari M.A., Oeltjen J.C., Schwartz S., Zhang Z., Muzny D.M., Lu J., Gorrell J.H., Chinault A.C., Belmont J.W., Miller W., Gibbs R.A.
Genome Res. 8:29-40(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Strain: C57BL/6J and NOD.
Tissue: Small intestine and Thymus.
[3]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
Strain: Czech II and FVB/N.
Tissue: Mammary tumor.
[5]"Tome-1, a trigger of mitotic entry, is degraded during G1 via the APC."
Ayad N.G., Rankin S., Murakami M., Jebanathirajah J., Gygi S.P., Kirschner M.W.
Cell 113:101-113(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH SKP1.
[6]"The phagosomal proteome in interferon-gamma-activated macrophages."
Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-29 AND SER-67, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AK008606 mRNA. Translation: BAB25773.1.
AK010409 mRNA. Translation: BAB26916.1.
AK011313 mRNA. Translation: BAB27539.1.
AK028159 mRNA. Translation: BAC25785.1.
AK145537 mRNA. Translation: BAE26492.1.
AK169792 mRNA. Translation: BAE41370.1.
AC002397 Genomic DNA. Translation: AAC36014.1.
BC002006 mRNA. Translation: AAH02006.1.
BC027172 mRNA. Translation: AAH27172.1. Different initiation.
CCDSCCDS39629.1. [Q99M54-1]
RefSeqNP_038566.1. NM_013538.5. [Q99M54-1]
XP_006505627.1. XM_006505564.1. [Q99M54-1]
UniGeneMm.285723.

3D structure databases

ModBaseSearch...
MobiDBSearch...

PTM databases

PhosphoSiteQ99M54.

Proteomic databases

PaxDbQ99M54.
PRIDEQ99M54.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000024270; ENSMUSP00000024270; ENSMUSG00000023505. [Q99M54-1]
GeneID14793.
KEGGmmu:14793.
UCSCuc009dsc.2. mouse. [Q99M54-1]

Organism-specific databases

CTD83461.
MGIMGI:1315198. Cdca3.

Phylogenomic databases

eggNOGNOG46427.
GeneTreeENSGT00390000017343.
HOGENOMHOG000013100.
HOVERGENHBG053225.
InParanoidQ99M54.
OMAKTGGRAW.
PhylomeDBQ99M54.
TreeFamTF101068.

Enzyme and pathway databases

UniPathwayUPA00143.

Gene expression databases

ArrayExpressQ99M54.
BgeeQ99M54.
CleanExMM_CDCA3.
GenevestigatorQ99M54.

Family and domain databases

ProtoNetSearch...

Other

NextBio286939.
PROQ99M54.
SOURCESearch...

Entry information

Entry nameCDCA3_MOUSE
AccessionPrimary (citable) accession number: Q99M54
Secondary accession number(s): O88837, Q8R2V1
Entry history
Integrated into UniProtKB/Swiss-Prot: May 15, 2007
Last sequence update: May 15, 2007
Last modified: July 9, 2014
This is version 76 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot