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Q99M54

- CDCA3_MOUSE

UniProt

Q99M54 - CDCA3_MOUSE

Protein

Cell division cycle-associated protein 3

Gene

Cdca3

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 78 (01 Oct 2014)
      Sequence version 2 (15 May 2007)
      Previous versions | rss
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    Functioni

    F-box-like protein which is required for entry into mitosis. Acts by participating in E3 ligase complexes that mediate the ubiquitination and degradation of WEE1 kinase at G2/M phase By similarity.By similarity

    Pathwayi

    GO - Biological processi

    1. mitotic nuclear division Source: UniProtKB-KW
    2. protein ubiquitination Source: UniProtKB-UniPathway

    Keywords - Biological processi

    Cell cycle, Cell division, Mitosis, Ubl conjugation pathway

    Enzyme and pathway databases

    UniPathwayiUPA00143.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cell division cycle-associated protein 3
    Alternative name(s):
    Gene-rich cluster protein C8
    Trigger of mitotic entry protein 1
    Short name:
    TOME-1
    Gene namesi
    Name:Cdca3
    Synonyms:C8, Grcc8, Tome1
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 6

    Organism-specific databases

    MGIiMGI:1315198. Cdca3.

    Subcellular locationi

    Cytoplasmcytosol By similarity

    GO - Cellular componenti

    1. cytosol Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 266266Cell division cycle-associated protein 3PRO_0000287709Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei29 – 291Phosphoserine1 Publication
    Modified residuei31 – 311PhosphoserineBy similarity
    Modified residuei37 – 371PhosphothreonineBy similarity
    Modified residuei44 – 441PhosphoserineBy similarity
    Modified residuei67 – 671Phosphoserine1 Publication
    Modified residuei75 – 751PhosphothreonineBy similarity
    Modified residuei93 – 931PhosphoserineBy similarity
    Modified residuei197 – 1971PhosphoserineBy similarity
    Modified residuei200 – 2001PhosphothreonineBy similarity
    Modified residuei207 – 2071PhosphoserineBy similarity

    Post-translational modificationi

    Ubiquitinated and degraded by the APC/C-Cdh1 complex.By similarity

    Keywords - PTMi

    Phosphoprotein, Ubl conjugation

    Proteomic databases

    PaxDbiQ99M54.
    PRIDEiQ99M54.

    PTM databases

    PhosphoSiteiQ99M54.

    Expressioni

    Gene expression databases

    ArrayExpressiQ99M54.
    BgeeiQ99M54.
    CleanExiMM_CDCA3.
    GenevestigatoriQ99M54.

    Interactioni

    Subunit structurei

    Interacts with SKP1. Part of a SCF (SKP1-cullin-F-box) protein ligase complex.1 Publication

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni90 – 11930F-box-likeAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi256 – 2583KEN box

    Domaini

    The KEN box is required for the association with the APC/C-Cdh1 complex.By similarity

    Phylogenomic databases

    eggNOGiNOG46427.
    GeneTreeiENSGT00390000017343.
    HOGENOMiHOG000013100.
    HOVERGENiHBG053225.
    InParanoidiQ99M54.
    OMAiKTGGRAW.
    PhylomeDBiQ99M54.
    TreeFamiTF101068.

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q99M54-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MGSTQSVSGT PARPLPRNKQ VARVADPRSP SAGIQRTPIQ VESSPQPSLP    50
    AEQLNGLKQA QDPDPRSPTL GIARTPMKIS GPDPQCSLVK ELSEVLETEA 100
    SESISSPELA LPRETPLFYD LDLSSDPQLS PEDQLLPWSQ AELDPKQVFT 150
    KEEAKQSAET IAASQNSDKP SRDPETPQSS GSKRSRRKAN SKVLGRSPLT 200
    ILQDDNSPGT LTLRQGKRPS ALSENVKDLK EGVVLGTGRF LKAGGGAREP 250
    NQDHDKENQH FALLES 266
    Length:266
    Mass (Da):28,708
    Last modified:May 15, 2007 - v2
    Checksum:i8E02E77ACAAAC2F8
    GO
    Isoform 2 (identifier: Q99M54-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         83-84: DP → GK
         85-266: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:84
    Mass (Da):8,791
    Checksum:i4D3621305F809E22
    GO

    Sequence cautioni

    The sequence AAH27172.1 differs from that shown. Reason: Erroneous initiation.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti119 – 1191Y → S in AAH02006. (PubMed:15489334)Curated
    Sequence conflicti126 – 1261D → G in AAH02006. (PubMed:15489334)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei83 – 842DP → GK in isoform 2. 1 PublicationVSP_025606
    Alternative sequencei85 – 266182Missing in isoform 2. 1 PublicationVSP_025607Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK008606 mRNA. Translation: BAB25773.1.
    AK010409 mRNA. Translation: BAB26916.1.
    AK011313 mRNA. Translation: BAB27539.1.
    AK028159 mRNA. Translation: BAC25785.1.
    AK145537 mRNA. Translation: BAE26492.1.
    AK169792 mRNA. Translation: BAE41370.1.
    AC002397 Genomic DNA. Translation: AAC36014.1.
    BC002006 mRNA. Translation: AAH02006.1.
    BC027172 mRNA. Translation: AAH27172.1. Different initiation.
    CCDSiCCDS39629.1. [Q99M54-1]
    RefSeqiNP_038566.1. NM_013538.5. [Q99M54-1]
    XP_006505627.1. XM_006505564.1. [Q99M54-1]
    UniGeneiMm.285723.

    Genome annotation databases

    EnsembliENSMUST00000024270; ENSMUSP00000024270; ENSMUSG00000023505. [Q99M54-1]
    GeneIDi14793.
    KEGGimmu:14793.
    UCSCiuc009dsc.2. mouse. [Q99M54-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK008606 mRNA. Translation: BAB25773.1 .
    AK010409 mRNA. Translation: BAB26916.1 .
    AK011313 mRNA. Translation: BAB27539.1 .
    AK028159 mRNA. Translation: BAC25785.1 .
    AK145537 mRNA. Translation: BAE26492.1 .
    AK169792 mRNA. Translation: BAE41370.1 .
    AC002397 Genomic DNA. Translation: AAC36014.1 .
    BC002006 mRNA. Translation: AAH02006.1 .
    BC027172 mRNA. Translation: AAH27172.1 . Different initiation.
    CCDSi CCDS39629.1. [Q99M54-1 ]
    RefSeqi NP_038566.1. NM_013538.5. [Q99M54-1 ]
    XP_006505627.1. XM_006505564.1. [Q99M54-1 ]
    UniGenei Mm.285723.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    PTM databases

    PhosphoSitei Q99M54.

    Proteomic databases

    PaxDbi Q99M54.
    PRIDEi Q99M54.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000024270 ; ENSMUSP00000024270 ; ENSMUSG00000023505 . [Q99M54-1 ]
    GeneIDi 14793.
    KEGGi mmu:14793.
    UCSCi uc009dsc.2. mouse. [Q99M54-1 ]

    Organism-specific databases

    CTDi 83461.
    MGIi MGI:1315198. Cdca3.

    Phylogenomic databases

    eggNOGi NOG46427.
    GeneTreei ENSGT00390000017343.
    HOGENOMi HOG000013100.
    HOVERGENi HBG053225.
    InParanoidi Q99M54.
    OMAi KTGGRAW.
    PhylomeDBi Q99M54.
    TreeFami TF101068.

    Enzyme and pathway databases

    UniPathwayi UPA00143 .

    Miscellaneous databases

    NextBioi 286939.
    PROi Q99M54.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q99M54.
    Bgeei Q99M54.
    CleanExi MM_CDCA3.
    Genevestigatori Q99M54.

    Family and domain databases

    ProtoNeti Search...

    Publicationsi

    1. "Comparative sequence analysis of a gene-rich cluster at human chromosome 12p13 and its syntenic region in mouse chromosome 6."
      Ansari-Lari M.A., Oeltjen J.C., Schwartz S., Zhang Z., Muzny D.M., Lu J., Gorrell J.H., Chinault A.C., Belmont J.W., Miller W., Gibbs R.A.
      Genome Res. 8:29-40(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Strain: C57BL/6J and NOD.
      Tissue: Small intestine and Thymus.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
      Strain: Czech II and FVB/N.
      Tissue: Mammary tumor.
    5. "Tome-1, a trigger of mitotic entry, is degraded during G1 via the APC."
      Ayad N.G., Rankin S., Murakami M., Jebanathirajah J., Gygi S.P., Kirschner M.W.
      Cell 113:101-113(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH SKP1.
    6. "The phagosomal proteome in interferon-gamma-activated macrophages."
      Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
      Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-29 AND SER-67, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiCDCA3_MOUSE
    AccessioniPrimary (citable) accession number: Q99M54
    Secondary accession number(s): O88837, Q8R2V1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 15, 2007
    Last sequence update: May 15, 2007
    Last modified: October 1, 2014
    This is version 78 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways

    External Data

    Dasty 3