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Protein

Cell division cycle-associated protein 3

Gene

Cdca3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

F-box-like protein which is required for entry into mitosis. Acts by participating in E3 ligase complexes that mediate the ubiquitination and degradation of WEE1 kinase at G2/M phase (By similarity).By similarity

Pathwayi

GO - Biological processi

  1. cell division Source: UniProtKB-KW
  2. mitotic nuclear division Source: UniProtKB-KW
  3. protein ubiquitination Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Biological processi

Cell cycle, Cell division, Mitosis, Ubl conjugation pathway

Enzyme and pathway databases

UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
Cell division cycle-associated protein 3
Alternative name(s):
Gene-rich cluster protein C8
Trigger of mitotic entry protein 1
Short name:
TOME-1
Gene namesi
Name:Cdca3
Synonyms:C8, Grcc8, Tome1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 6

Organism-specific databases

MGIiMGI:1315198. Cdca3.

Subcellular locationi

Cytoplasmcytosol By similarity

GO - Cellular componenti

  1. cytosol Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 266266Cell division cycle-associated protein 3PRO_0000287709Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei29 – 291Phosphoserine1 Publication
Modified residuei31 – 311PhosphoserineBy similarity
Modified residuei37 – 371PhosphothreonineBy similarity
Modified residuei44 – 441PhosphoserineBy similarity
Modified residuei67 – 671Phosphoserine1 Publication
Modified residuei75 – 751PhosphothreonineBy similarity
Modified residuei197 – 1971PhosphoserineBy similarity
Modified residuei200 – 2001PhosphothreonineBy similarity
Modified residuei207 – 2071PhosphoserineBy similarity

Post-translational modificationi

Ubiquitinated and degraded by the APC/C-Cdh1 complex.By similarity

Keywords - PTMi

Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiQ99M54.
PaxDbiQ99M54.
PRIDEiQ99M54.

PTM databases

PhosphoSiteiQ99M54.

Expressioni

Gene expression databases

BgeeiQ99M54.
CleanExiMM_CDCA3.
ExpressionAtlasiQ99M54. baseline and differential.
GenevestigatoriQ99M54.

Interactioni

Subunit structurei

Interacts with SKP1. Part of a SCF (SKP1-cullin-F-box) protein ligase complex.1 Publication

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni90 – 11930F-box-likeAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi256 – 2583KEN box

Domaini

The KEN box is required for the association with the APC/C-Cdh1 complex.By similarity

Phylogenomic databases

eggNOGiNOG46427.
GeneTreeiENSGT00390000017343.
HOGENOMiHOG000013100.
HOVERGENiHBG053225.
InParanoidiQ99M54.
OMAiKENQHFA.
PhylomeDBiQ99M54.
TreeFamiTF101068.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q99M54-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGSTQSVSGT PARPLPRNKQ VARVADPRSP SAGIQRTPIQ VESSPQPSLP
60 70 80 90 100
AEQLNGLKQA QDPDPRSPTL GIARTPMKIS GPDPQCSLVK ELSEVLETEA
110 120 130 140 150
SESISSPELA LPRETPLFYD LDLSSDPQLS PEDQLLPWSQ AELDPKQVFT
160 170 180 190 200
KEEAKQSAET IAASQNSDKP SRDPETPQSS GSKRSRRKAN SKVLGRSPLT
210 220 230 240 250
ILQDDNSPGT LTLRQGKRPS ALSENVKDLK EGVVLGTGRF LKAGGGAREP
260
NQDHDKENQH FALLES
Length:266
Mass (Da):28,708
Last modified:May 15, 2007 - v2
Checksum:i8E02E77ACAAAC2F8
GO
Isoform 2 (identifier: Q99M54-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     83-84: DP → GK
     85-266: Missing.

Note: No experimental confirmation available.

Show »
Length:84
Mass (Da):8,791
Checksum:i4D3621305F809E22
GO

Sequence cautioni

The sequence AAH27172.1 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti119 – 1191Y → S in AAH02006 (PubMed:15489334).Curated
Sequence conflicti126 – 1261D → G in AAH02006 (PubMed:15489334).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei83 – 842DP → GK in isoform 2. 1 PublicationVSP_025606
Alternative sequencei85 – 266182Missing in isoform 2. 1 PublicationVSP_025607Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK008606 mRNA. Translation: BAB25773.1.
AK010409 mRNA. Translation: BAB26916.1.
AK011313 mRNA. Translation: BAB27539.1.
AK028159 mRNA. Translation: BAC25785.1.
AK145537 mRNA. Translation: BAE26492.1.
AK169792 mRNA. Translation: BAE41370.1.
AC002397 Genomic DNA. Translation: AAC36014.1.
BC002006 mRNA. Translation: AAH02006.1.
BC027172 mRNA. Translation: AAH27172.1. Different initiation.
CCDSiCCDS39629.1. [Q99M54-1]
RefSeqiNP_038566.1. NM_013538.5. [Q99M54-1]
XP_006505627.1. XM_006505564.2. [Q99M54-1]
UniGeneiMm.285723.

Genome annotation databases

EnsembliENSMUST00000024270; ENSMUSP00000024270; ENSMUSG00000023505. [Q99M54-1]
GeneIDi14793.
KEGGimmu:14793.
UCSCiuc009dsc.2. mouse. [Q99M54-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK008606 mRNA. Translation: BAB25773.1.
AK010409 mRNA. Translation: BAB26916.1.
AK011313 mRNA. Translation: BAB27539.1.
AK028159 mRNA. Translation: BAC25785.1.
AK145537 mRNA. Translation: BAE26492.1.
AK169792 mRNA. Translation: BAE41370.1.
AC002397 Genomic DNA. Translation: AAC36014.1.
BC002006 mRNA. Translation: AAH02006.1.
BC027172 mRNA. Translation: AAH27172.1. Different initiation.
CCDSiCCDS39629.1. [Q99M54-1]
RefSeqiNP_038566.1. NM_013538.5. [Q99M54-1]
XP_006505627.1. XM_006505564.2. [Q99M54-1]
UniGeneiMm.285723.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

PTM databases

PhosphoSiteiQ99M54.

Proteomic databases

MaxQBiQ99M54.
PaxDbiQ99M54.
PRIDEiQ99M54.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000024270; ENSMUSP00000024270; ENSMUSG00000023505. [Q99M54-1]
GeneIDi14793.
KEGGimmu:14793.
UCSCiuc009dsc.2. mouse. [Q99M54-1]

Organism-specific databases

CTDi83461.
MGIiMGI:1315198. Cdca3.

Phylogenomic databases

eggNOGiNOG46427.
GeneTreeiENSGT00390000017343.
HOGENOMiHOG000013100.
HOVERGENiHBG053225.
InParanoidiQ99M54.
OMAiKENQHFA.
PhylomeDBiQ99M54.
TreeFamiTF101068.

Enzyme and pathway databases

UniPathwayiUPA00143.

Miscellaneous databases

NextBioi286939.
PROiQ99M54.
SOURCEiSearch...

Gene expression databases

BgeeiQ99M54.
CleanExiMM_CDCA3.
ExpressionAtlasiQ99M54. baseline and differential.
GenevestigatoriQ99M54.

Family and domain databases

ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Comparative sequence analysis of a gene-rich cluster at human chromosome 12p13 and its syntenic region in mouse chromosome 6."
    Ansari-Lari M.A., Oeltjen J.C., Schwartz S., Zhang Z., Muzny D.M., Lu J., Gorrell J.H., Chinault A.C., Belmont J.W., Miller W., Gibbs R.A.
    Genome Res. 8:29-40(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: C57BL/6J and NOD.
    Tissue: Small intestine and Thymus.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Strain: Czech II and FVB/N.
    Tissue: Mammary tumor.
  5. "Tome-1, a trigger of mitotic entry, is degraded during G1 via the APC."
    Ayad N.G., Rankin S., Murakami M., Jebanathirajah J., Gygi S.P., Kirschner M.W.
    Cell 113:101-113(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH SKP1.
  6. "The phagosomal proteome in interferon-gamma-activated macrophages."
    Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
    Immunity 30:143-154(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-29 AND SER-67, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiCDCA3_MOUSE
AccessioniPrimary (citable) accession number: Q99M54
Secondary accession number(s): O88837, Q8R2V1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 15, 2007
Last sequence update: May 15, 2007
Last modified: April 1, 2015
This is version 83 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.