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Q99M51

- NCK1_MOUSE

UniProt

Q99M51 - NCK1_MOUSE

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Protein
Cytoplasmic protein NCK1
Gene
Nck1
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Adapter protein which associates with tyrosine-phosphorylated growth factor receptors, such as KDR and PDGFRB, or their cellular substrates. Maintains low levels of EIF2S1 phosphorylation by promoting its dephosphorylation by PP1. Plays a role in the DNA damage response, not in the detection of the damage by ATM/ATR, but for efficient activation of downstream effectors, such as that of CHEK2. Plays a role in ELK1-dependent transcriptional activation in response to activated Ras signaling. Modulates the activation of EIF2AK2/PKR by dsRNA By similarity.

GO - Molecular functioni

  1. protein binding Source: IntAct
  2. protein domain specific binding Source: MGI
  3. protein kinase inhibitor activity Source: UniProtKB

GO - Biological processi

  1. T cell activation Source: Ensembl
  2. actin filament organization Source: MGI
  3. cell migration Source: MGI
  4. lamellipodium assembly Source: MGI
  5. negative regulation of cell death Source: UniProtKB
  6. positive regulation of T cell proliferation Source: Ensembl
  7. positive regulation of actin filament polymerization Source: MGI
  8. positive regulation of transcription from RNA polymerase II promoter Source: UniProtKB
  9. regulation of translation Source: UniProtKB-KW
  10. response to other organism Source: MGI
Complete GO annotation...

Keywords - Biological processi

Translation regulation

Enzyme and pathway databases

ReactomeiREACT_188194. Antigen activates B Cell Receptor (BCR) leading to generation of second messengers.
REACT_198374. Regulation of actin dynamics for phagocytic cup formation.
REACT_225768. Generation of second messenger molecules.

Names & Taxonomyi

Protein namesi
Recommended name:
Cytoplasmic protein NCK1
Alternative name(s):
NCK adaptor protein 1
Short name:
Nck-1
Gene namesi
Name:Nck1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 9

Organism-specific databases

MGIiMGI:109601. Nck1.

Subcellular locationi

Cytoplasm By similarity. Endoplasmic reticulum By similarity. Nucleus By similarity
Note: Mostly cytoplasmic, but shuttles between the cytoplasm and the nucleus. Import into the nucleus requires interaction with SOCS7. Predominantly nuclear following genotoxic stresses, such as UV irradiation, hydroxyurea or mitomycin C treatments By similarity.

GO - Cellular componenti

  1. cell-cell junction Source: MGI
  2. endoplasmic reticulum Source: UniProtKB-SubCell
  3. nucleus Source: UniProtKB-SubCell
  4. vesicle membrane Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Endoplasmic reticulum, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed By similarity
Chaini2 – 377376Cytoplasmic protein NCK1
PRO_0000413801Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanine By similarity
Modified residuei85 – 851Phosphoserine By similarity
Modified residuei91 – 911Phosphoserine By similarity
Modified residuei96 – 961Phosphoserine By similarity
Modified residuei105 – 1051Phosphotyrosine1 Publication

Post-translational modificationi

Phosphorylated on Ser and Tyr residues. Phosphorylated in response to activation of EGFR and FcERI. Phosphorylated by activated PDGFRB By similarity.

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiQ99M51.
PRIDEiQ99M51.

2D gel databases

REPRODUCTION-2DPAGEIPI00453999.

PTM databases

PhosphoSiteiQ99M51.

Expressioni

Gene expression databases

ArrayExpressiQ99M51.
BgeeiQ99M51.
GenevestigatoriQ99M51.

Interactioni

Subunit structurei

Associates with BLNK, PLCG1, VAV1 and NCK1 in a B-cell antigen receptor-dependent fashion. Interacts with SOCS7. This interaction is required for nuclear import. Part of a complex containing PPP1R15B, PP1 and NCK1. Interacts with RALGPS1. Interacts with CAV2 (tyrosine phosphorylated form). Interacts with ADAM15. Interacts with FASLG. Directly interacts with RASA1. Interacts with MINK1. Interacts with KDR (tyrosine phosphorylated). Interacts (via SH2 domain) with EPHB1; activates the JUN cascade to regulate cell adhesion. Interacts with EPHA2. Interacts (via SH2 domain) with PDGFRB (tyrosine phosphorylated). Interacts (via SH2 domain and SH3 domain 2) with EGFR. Interacts with PAK1 and SOS1. Interacts (via SH3 domains) with PKN2 By similarity. Interacts with FLT1 (tyrosine phosphorylated). Interacts with the inactive form of EIF2AK2/PKR By similarity.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
Abl1P005202EBI-642202,EBI-914519
Dok1P974655EBI-642202,EBI-914917

Protein-protein interaction databases

BioGridi201704. 5 interactions.
IntActiQ99M51. 15 interactions.
MINTiMINT-193264.
STRINGi10090.ENSMUSP00000112221.

Structurei

3D structure databases

ProteinModelPortaliQ99M51.
SMRiQ99M51. Positions 1-61, 99-174, 193-250, 279-377.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini2 – 6160SH3 1
Add
BLAST
Domaini106 – 16560SH3 2
Add
BLAST
Domaini190 – 25263SH3 3
Add
BLAST
Domaini282 – 37695SH2
Add
BLAST

Domaini

Only the first and third SH3 domains seem to be involved in RASA1-binding; the second SH3 domain and the SH2 domains do not seem to be involved By similarity.

Sequence similaritiesi

Contains 1 SH2 domain.
Contains 3 SH3 domains.

Keywords - Domaini

Repeat, SH2 domain, SH3 domain

Phylogenomic databases

eggNOGiNOG261435.
GeneTreeiENSGT00550000074482.
HOGENOMiHOG000290684.
HOVERGENiHBG000719.
InParanoidiQ9Z279.
KOiK07365.
OMAiTIMQNNP.
OrthoDBiEOG7SV0VJ.
PhylomeDBiQ99M51.
TreeFamiTF351631.

Family and domain databases

Gene3Di3.30.505.10. 1 hit.
InterProiIPR017304. NCK.
IPR028526. NCK1.
IPR000980. SH2.
IPR001452. SH3_domain.
[Graphical view]
PANTHERiPTHR22820:SF11. PTHR22820:SF11. 1 hit.
PfamiPF00017. SH2. 1 hit.
PF00018. SH3_1. 2 hits.
PF14604. SH3_9. 1 hit.
[Graphical view]
PIRSFiPIRSF037874. Cytoplasmic_NCK. 1 hit.
PRINTSiPR00401. SH2DOMAIN.
PR00452. SH3DOMAIN.
SMARTiSM00252. SH2. 1 hit.
SM00326. SH3. 3 hits.
[Graphical view]
SUPFAMiSSF50044. SSF50044. 3 hits.
SSF55550. SSF55550. 1 hit.
PROSITEiPS50001. SH2. 1 hit.
PS50002. SH3. 3 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q99M51-1 [UniParc]FASTAAdd to Basket

« Hide

MAEEVVVVAK FDYVAQQEQE LDIKKNERLW LLDDSKSWWR VRNSMNKTGF    50
VPSNYVERKN SARKASIVKN LKDTLGIGKV KRKPSVPDTA SPADDSFVDP 100
GERLYDLNMP AFVKFNYMAE REDELSLIKG TKVIVMEKCS DGWWRGSYNG 150
QIGWFPSNYV TEEGDSPLGD HVGSLSEKLA AVVNNLNTGQ VLHVVQALYP 200
FSSSNDEELN FEKGDVMDVI EKPENDPEWW KCRKINGMVG LVPKNYVTIM 250
QNNPLTSGLE PSPPQCDYIR PSLTGKFAGN PWYYGKVTRH QAEMALNERG 300
HEGDFLIRDS ESSPNDFSVS LKAQGKNKHF KVQLKETVYC IGQRKFSTME 350
ELVEHYKKAP IFTSEQGEKL YLVKHLS 377
Length:377
Mass (Da):42,891
Last modified:June 1, 2001 - v1
Checksum:i671BBFC870A88EB9
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti4 – 41E → G in AAD13752. 1 Publication
Sequence conflicti4 – 41E → G in AAC06352. 1 Publication
Sequence conflicti30 – 301W → C in AAC06352. 1 Publication
Sequence conflicti74 – 741T → N in AAC06352. 1 Publication
Sequence conflicti84 – 841P → T in AAC06352. 1 Publication
Sequence conflicti90 – 901A → E in AAC06352. 1 Publication
Sequence conflicti155 – 1573FPS → LPC in AAC06352. 1 Publication
Sequence conflicti202 – 2021S → G in AAC06352. 1 Publication
Sequence conflicti206 – 2061D → N in AAC06352. 1 Publication
Sequence conflicti307 – 3071I → V in AAC06352. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF084183 mRNA. Translation: AAD13752.1.
AF043259 mRNA. Translation: AAC06352.1.
AC129223 Genomic DNA. No translation available.
CH466560 Genomic DNA. Translation: EDL21028.1.
CH466560 Genomic DNA. Translation: EDL21029.1.
CH466560 Genomic DNA. Translation: EDL21031.1.
BC002015 mRNA. Translation: AAH02015.1.
CCDSiCCDS23440.1.
RefSeqiNP_035008.2. NM_010878.2.
XP_006510903.1. XM_006510840.1.
UniGeneiMm.181485.

Genome annotation databases

EnsembliENSMUST00000116522; ENSMUSP00000112221; ENSMUSG00000032475.
GeneIDi17973.
KEGGimmu:17973.
UCSCiuc009rex.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF084183 mRNA. Translation: AAD13752.1 .
AF043259 mRNA. Translation: AAC06352.1 .
AC129223 Genomic DNA. No translation available.
CH466560 Genomic DNA. Translation: EDL21028.1 .
CH466560 Genomic DNA. Translation: EDL21029.1 .
CH466560 Genomic DNA. Translation: EDL21031.1 .
BC002015 mRNA. Translation: AAH02015.1 .
CCDSi CCDS23440.1.
RefSeqi NP_035008.2. NM_010878.2.
XP_006510903.1. XM_006510840.1.
UniGenei Mm.181485.

3D structure databases

ProteinModelPortali Q99M51.
SMRi Q99M51. Positions 1-61, 99-174, 193-250, 279-377.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 201704. 5 interactions.
IntActi Q99M51. 15 interactions.
MINTi MINT-193264.
STRINGi 10090.ENSMUSP00000112221.

PTM databases

PhosphoSitei Q99M51.

2D gel databases

REPRODUCTION-2DPAGE IPI00453999.

Proteomic databases

MaxQBi Q99M51.
PRIDEi Q99M51.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000116522 ; ENSMUSP00000112221 ; ENSMUSG00000032475 .
GeneIDi 17973.
KEGGi mmu:17973.
UCSCi uc009rex.1. mouse.

Organism-specific databases

CTDi 4690.
MGIi MGI:109601. Nck1.

Phylogenomic databases

eggNOGi NOG261435.
GeneTreei ENSGT00550000074482.
HOGENOMi HOG000290684.
HOVERGENi HBG000719.
InParanoidi Q9Z279.
KOi K07365.
OMAi TIMQNNP.
OrthoDBi EOG7SV0VJ.
PhylomeDBi Q99M51.
TreeFami TF351631.

Enzyme and pathway databases

Reactomei REACT_188194. Antigen activates B Cell Receptor (BCR) leading to generation of second messengers.
REACT_198374. Regulation of actin dynamics for phagocytic cup formation.
REACT_225768. Generation of second messenger molecules.

Miscellaneous databases

NextBioi 292925.
PROi Q99M51.
SOURCEi Search...

Gene expression databases

ArrayExpressi Q99M51.
Bgeei Q99M51.
Genevestigatori Q99M51.

Family and domain databases

Gene3Di 3.30.505.10. 1 hit.
InterProi IPR017304. NCK.
IPR028526. NCK1.
IPR000980. SH2.
IPR001452. SH3_domain.
[Graphical view ]
PANTHERi PTHR22820:SF11. PTHR22820:SF11. 1 hit.
Pfami PF00017. SH2. 1 hit.
PF00018. SH3_1. 2 hits.
PF14604. SH3_9. 1 hit.
[Graphical view ]
PIRSFi PIRSF037874. Cytoplasmic_NCK. 1 hit.
PRINTSi PR00401. SH2DOMAIN.
PR00452. SH3DOMAIN.
SMARTi SM00252. SH2. 1 hit.
SM00326. SH3. 3 hits.
[Graphical view ]
SUPFAMi SSF50044. SSF50044. 3 hits.
SSF55550. SSF55550. 1 hit.
PROSITEi PS50001. SH2. 1 hit.
PS50002. SH3. 3 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Mus musculus SH2/SH3 adaptor protein (Nck) mRNA, complete cds."
    Miyoshi-Akiyama T., Mayer B.J.
    Submitted (AUG-1998) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. Chen M., She H.Y., Li W.
    Submitted (JAN-1998) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  4. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Mammary tumor.
  6. "Identification of vascular endothelial growth factor receptor-1 tyrosine phosphorylation sites and binding of SH2 domain-containing molecules."
    Ito N., Wernstedt C., Engstrom U., Claesson-Welsh L.
    J. Biol. Chem. 273:23410-23418(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH FLT1.
  7. "Large-scale identification and evolution indexing of tyrosine phosphorylation sites from murine brain."
    Ballif B.A., Carey G.R., Sunyaev S.R., Gygi S.P.
    J. Proteome Res. 7:311-318(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-105, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain.

Entry informationi

Entry nameiNCK1_MOUSE
AccessioniPrimary (citable) accession number: Q99M51
Secondary accession number(s): O55032, Q9Z279
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 16, 2011
Last sequence update: June 1, 2001
Last modified: September 3, 2014
This is version 106 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi