Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q99M51

- NCK1_MOUSE

UniProt

Q99M51 - NCK1_MOUSE

Protein

Cytoplasmic protein NCK1

Gene

Nck1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 107 (01 Oct 2014)
      Sequence version 1 (01 Jun 2001)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Adapter protein which associates with tyrosine-phosphorylated growth factor receptors, such as KDR and PDGFRB, or their cellular substrates. Maintains low levels of EIF2S1 phosphorylation by promoting its dephosphorylation by PP1. Plays a role in the DNA damage response, not in the detection of the damage by ATM/ATR, but for efficient activation of downstream effectors, such as that of CHEK2. Plays a role in ELK1-dependent transcriptional activation in response to activated Ras signaling. Modulates the activation of EIF2AK2/PKR by dsRNA By similarity.By similarity

    GO - Molecular functioni

    1. protein binding Source: IntAct
    2. protein domain specific binding Source: MGI
    3. protein kinase inhibitor activity Source: UniProtKB

    GO - Biological processi

    1. actin filament organization Source: MGI
    2. cell migration Source: MGI
    3. lamellipodium assembly Source: MGI
    4. negative regulation of cell death Source: UniProtKB
    5. positive regulation of actin filament polymerization Source: MGI
    6. positive regulation of T cell proliferation Source: Ensembl
    7. positive regulation of transcription from RNA polymerase II promoter Source: UniProtKB
    8. regulation of translation Source: UniProtKB-KW
    9. response to other organism Source: MGI
    10. T cell activation Source: Ensembl

    Keywords - Biological processi

    Translation regulation

    Enzyme and pathway databases

    ReactomeiREACT_188194. Antigen activates B Cell Receptor (BCR) leading to generation of second messengers.
    REACT_198374. Regulation of actin dynamics for phagocytic cup formation.
    REACT_225768. Generation of second messenger molecules.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cytoplasmic protein NCK1
    Alternative name(s):
    NCK adaptor protein 1
    Short name:
    Nck-1
    Gene namesi
    Name:Nck1
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 9

    Organism-specific databases

    MGIiMGI:109601. Nck1.

    Subcellular locationi

    Cytoplasm By similarity. Endoplasmic reticulum By similarity. Nucleus By similarity
    Note: Mostly cytoplasmic, but shuttles between the cytoplasm and the nucleus. Import into the nucleus requires interaction with SOCS7. Predominantly nuclear following genotoxic stresses, such as UV irradiation, hydroxyurea or mitomycin C treatments By similarity.By similarity

    GO - Cellular componenti

    1. cell-cell junction Source: MGI
    2. endoplasmic reticulum Source: UniProtKB-SubCell
    3. nucleus Source: UniProtKB-SubCell
    4. vesicle membrane Source: MGI

    Keywords - Cellular componenti

    Cytoplasm, Endoplasmic reticulum, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 377376Cytoplasmic protein NCK1PRO_0000413801Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylalanineBy similarity
    Modified residuei85 – 851PhosphoserineBy similarity
    Modified residuei91 – 911PhosphoserineBy similarity
    Modified residuei96 – 961PhosphoserineBy similarity
    Modified residuei105 – 1051Phosphotyrosine1 Publication

    Post-translational modificationi

    Phosphorylated on Ser and Tyr residues. Phosphorylated in response to activation of EGFR and FcERI. Phosphorylated by activated PDGFRB By similarity.By similarity

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    MaxQBiQ99M51.
    PRIDEiQ99M51.

    2D gel databases

    REPRODUCTION-2DPAGEIPI00453999.

    PTM databases

    PhosphoSiteiQ99M51.

    Expressioni

    Gene expression databases

    ArrayExpressiQ99M51.
    BgeeiQ99M51.
    GenevestigatoriQ99M51.

    Interactioni

    Subunit structurei

    Associates with BLNK, PLCG1, VAV1 and NCK1 in a B-cell antigen receptor-dependent fashion. Interacts with SOCS7. This interaction is required for nuclear import. Part of a complex containing PPP1R15B, PP1 and NCK1. Interacts with RALGPS1. Interacts with CAV2 (tyrosine phosphorylated form). Interacts with ADAM15. Interacts with FASLG. Directly interacts with RASA1. Interacts with MINK1. Interacts with KDR (tyrosine phosphorylated). Interacts (via SH2 domain) with EPHB1; activates the JUN cascade to regulate cell adhesion. Interacts with EPHA2. Interacts (via SH2 domain) with PDGFRB (tyrosine phosphorylated). Interacts (via SH2 domain and SH3 domain 2) with EGFR. Interacts with PAK1 and SOS1. Interacts (via SH3 domains) with PKN2 By similarity. Interacts with FLT1 (tyrosine phosphorylated). Interacts with the inactive form of EIF2AK2/PKR By similarity.By similarity

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    Abl1P005202EBI-642202,EBI-914519
    Dok1P974655EBI-642202,EBI-914917

    Protein-protein interaction databases

    BioGridi201704. 5 interactions.
    IntActiQ99M51. 15 interactions.
    MINTiMINT-193264.
    STRINGi10090.ENSMUSP00000112221.

    Structurei

    3D structure databases

    ProteinModelPortaliQ99M51.
    SMRiQ99M51. Positions 1-61, 99-174, 193-250, 279-377.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini2 – 6160SH3 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini106 – 16560SH3 2PROSITE-ProRule annotationAdd
    BLAST
    Domaini190 – 25263SH3 3PROSITE-ProRule annotationAdd
    BLAST
    Domaini282 – 37695SH2PROSITE-ProRule annotationAdd
    BLAST

    Domaini

    Only the first and third SH3 domains seem to be involved in RASA1-binding; the second SH3 domain and the SH2 domains do not seem to be involved.By similarity

    Sequence similaritiesi

    Contains 1 SH2 domain.PROSITE-ProRule annotation
    Contains 3 SH3 domains.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat, SH2 domain, SH3 domain

    Phylogenomic databases

    eggNOGiNOG261435.
    GeneTreeiENSGT00550000074482.
    HOGENOMiHOG000290684.
    HOVERGENiHBG000719.
    InParanoidiQ9Z279.
    KOiK07365.
    OMAiTIMQNNP.
    OrthoDBiEOG7SV0VJ.
    PhylomeDBiQ99M51.
    TreeFamiTF351631.

    Family and domain databases

    Gene3Di3.30.505.10. 1 hit.
    InterProiIPR017304. NCK.
    IPR028526. NCK1.
    IPR000980. SH2.
    IPR001452. SH3_domain.
    [Graphical view]
    PANTHERiPTHR22820:SF11. PTHR22820:SF11. 1 hit.
    PfamiPF00017. SH2. 1 hit.
    PF00018. SH3_1. 2 hits.
    PF14604. SH3_9. 1 hit.
    [Graphical view]
    PIRSFiPIRSF037874. Cytoplasmic_NCK. 1 hit.
    PRINTSiPR00401. SH2DOMAIN.
    PR00452. SH3DOMAIN.
    SMARTiSM00252. SH2. 1 hit.
    SM00326. SH3. 3 hits.
    [Graphical view]
    SUPFAMiSSF50044. SSF50044. 3 hits.
    SSF55550. SSF55550. 1 hit.
    PROSITEiPS50001. SH2. 1 hit.
    PS50002. SH3. 3 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q99M51-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAEEVVVVAK FDYVAQQEQE LDIKKNERLW LLDDSKSWWR VRNSMNKTGF    50
    VPSNYVERKN SARKASIVKN LKDTLGIGKV KRKPSVPDTA SPADDSFVDP 100
    GERLYDLNMP AFVKFNYMAE REDELSLIKG TKVIVMEKCS DGWWRGSYNG 150
    QIGWFPSNYV TEEGDSPLGD HVGSLSEKLA AVVNNLNTGQ VLHVVQALYP 200
    FSSSNDEELN FEKGDVMDVI EKPENDPEWW KCRKINGMVG LVPKNYVTIM 250
    QNNPLTSGLE PSPPQCDYIR PSLTGKFAGN PWYYGKVTRH QAEMALNERG 300
    HEGDFLIRDS ESSPNDFSVS LKAQGKNKHF KVQLKETVYC IGQRKFSTME 350
    ELVEHYKKAP IFTSEQGEKL YLVKHLS 377
    Length:377
    Mass (Da):42,891
    Last modified:June 1, 2001 - v1
    Checksum:i671BBFC870A88EB9
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti4 – 41E → G in AAD13752. 1 PublicationCurated
    Sequence conflicti4 – 41E → G in AAC06352. 1 PublicationCurated
    Sequence conflicti30 – 301W → C in AAC06352. 1 PublicationCurated
    Sequence conflicti74 – 741T → N in AAC06352. 1 PublicationCurated
    Sequence conflicti84 – 841P → T in AAC06352. 1 PublicationCurated
    Sequence conflicti90 – 901A → E in AAC06352. 1 PublicationCurated
    Sequence conflicti155 – 1573FPS → LPC in AAC06352. 1 PublicationCurated
    Sequence conflicti202 – 2021S → G in AAC06352. 1 PublicationCurated
    Sequence conflicti206 – 2061D → N in AAC06352. 1 PublicationCurated
    Sequence conflicti307 – 3071I → V in AAC06352. 1 PublicationCurated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF084183 mRNA. Translation: AAD13752.1.
    AF043259 mRNA. Translation: AAC06352.1.
    AC129223 Genomic DNA. No translation available.
    CH466560 Genomic DNA. Translation: EDL21028.1.
    CH466560 Genomic DNA. Translation: EDL21029.1.
    CH466560 Genomic DNA. Translation: EDL21031.1.
    BC002015 mRNA. Translation: AAH02015.1.
    CCDSiCCDS23440.1.
    RefSeqiNP_035008.2. NM_010878.2.
    XP_006510903.1. XM_006510840.1.
    UniGeneiMm.181485.

    Genome annotation databases

    EnsembliENSMUST00000116522; ENSMUSP00000112221; ENSMUSG00000032475.
    GeneIDi17973.
    KEGGimmu:17973.
    UCSCiuc009rex.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF084183 mRNA. Translation: AAD13752.1 .
    AF043259 mRNA. Translation: AAC06352.1 .
    AC129223 Genomic DNA. No translation available.
    CH466560 Genomic DNA. Translation: EDL21028.1 .
    CH466560 Genomic DNA. Translation: EDL21029.1 .
    CH466560 Genomic DNA. Translation: EDL21031.1 .
    BC002015 mRNA. Translation: AAH02015.1 .
    CCDSi CCDS23440.1.
    RefSeqi NP_035008.2. NM_010878.2.
    XP_006510903.1. XM_006510840.1.
    UniGenei Mm.181485.

    3D structure databases

    ProteinModelPortali Q99M51.
    SMRi Q99M51. Positions 1-61, 99-174, 193-250, 279-377.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 201704. 5 interactions.
    IntActi Q99M51. 15 interactions.
    MINTi MINT-193264.
    STRINGi 10090.ENSMUSP00000112221.

    PTM databases

    PhosphoSitei Q99M51.

    2D gel databases

    REPRODUCTION-2DPAGE IPI00453999.

    Proteomic databases

    MaxQBi Q99M51.
    PRIDEi Q99M51.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000116522 ; ENSMUSP00000112221 ; ENSMUSG00000032475 .
    GeneIDi 17973.
    KEGGi mmu:17973.
    UCSCi uc009rex.1. mouse.

    Organism-specific databases

    CTDi 4690.
    MGIi MGI:109601. Nck1.

    Phylogenomic databases

    eggNOGi NOG261435.
    GeneTreei ENSGT00550000074482.
    HOGENOMi HOG000290684.
    HOVERGENi HBG000719.
    InParanoidi Q9Z279.
    KOi K07365.
    OMAi TIMQNNP.
    OrthoDBi EOG7SV0VJ.
    PhylomeDBi Q99M51.
    TreeFami TF351631.

    Enzyme and pathway databases

    Reactomei REACT_188194. Antigen activates B Cell Receptor (BCR) leading to generation of second messengers.
    REACT_198374. Regulation of actin dynamics for phagocytic cup formation.
    REACT_225768. Generation of second messenger molecules.

    Miscellaneous databases

    NextBioi 292925.
    PROi Q99M51.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q99M51.
    Bgeei Q99M51.
    Genevestigatori Q99M51.

    Family and domain databases

    Gene3Di 3.30.505.10. 1 hit.
    InterProi IPR017304. NCK.
    IPR028526. NCK1.
    IPR000980. SH2.
    IPR001452. SH3_domain.
    [Graphical view ]
    PANTHERi PTHR22820:SF11. PTHR22820:SF11. 1 hit.
    Pfami PF00017. SH2. 1 hit.
    PF00018. SH3_1. 2 hits.
    PF14604. SH3_9. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF037874. Cytoplasmic_NCK. 1 hit.
    PRINTSi PR00401. SH2DOMAIN.
    PR00452. SH3DOMAIN.
    SMARTi SM00252. SH2. 1 hit.
    SM00326. SH3. 3 hits.
    [Graphical view ]
    SUPFAMi SSF50044. SSF50044. 3 hits.
    SSF55550. SSF55550. 1 hit.
    PROSITEi PS50001. SH2. 1 hit.
    PS50002. SH3. 3 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Mus musculus SH2/SH3 adaptor protein (Nck) mRNA, complete cds."
      Miyoshi-Akiyama T., Mayer B.J.
      Submitted (AUG-1998) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. Chen M., She H.Y., Li W.
      Submitted (JAN-1998) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    4. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
      Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: FVB/N.
      Tissue: Mammary tumor.
    6. "Identification of vascular endothelial growth factor receptor-1 tyrosine phosphorylation sites and binding of SH2 domain-containing molecules."
      Ito N., Wernstedt C., Engstrom U., Claesson-Welsh L.
      J. Biol. Chem. 273:23410-23418(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH FLT1.
    7. "Large-scale identification and evolution indexing of tyrosine phosphorylation sites from murine brain."
      Ballif B.A., Carey G.R., Sunyaev S.R., Gygi S.P.
      J. Proteome Res. 7:311-318(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-105, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Brain.

    Entry informationi

    Entry nameiNCK1_MOUSE
    AccessioniPrimary (citable) accession number: Q99M51
    Secondary accession number(s): O55032, Q9Z279
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 16, 2011
    Last sequence update: June 1, 2001
    Last modified: October 1, 2014
    This is version 107 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3