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Q99M04

- LIAS_MOUSE

UniProt

Q99M04 - LIAS_MOUSE

Protein

Lipoyl synthase, mitochondrial

Gene

Lias

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 109 (01 Oct 2014)
      Sequence version 1 (01 Jun 2001)
      Previous versions | rss
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    Functioni

    Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives.Curated

    Catalytic activityi

    Protein N(6)-(octanoyl)lysine + 2 sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = protein N(6)-(lipoyl)lysine + 2 (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.

    Cofactori

    Binds 2 4Fe-4S clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi105 – 1051Iron-sulfur 1 (4Fe-4S)UniRule annotation
    Metal bindingi110 – 1101Iron-sulfur 1 (4Fe-4S)UniRule annotation
    Metal bindingi116 – 1161Iron-sulfur 1 (4Fe-4S)UniRule annotation
    Metal bindingi136 – 1361Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi140 – 1401Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi143 – 1431Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation

    GO - Molecular functioni

    1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
    2. lipoate synthase activity Source: MGI
    3. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. inflammatory response Source: MGI
    2. lipoate biosynthetic process Source: MGI
    3. neural tube closure Source: MGI
    4. protein lipoylation Source: UniProtKB-HAMAP
    5. response to lipopolysaccharide Source: MGI
    6. response to oxidative stress Source: MGI

    Keywords - Molecular functioni

    Transferase

    Keywords - Ligandi

    4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

    Enzyme and pathway databases

    UniPathwayiUPA00538; UER00593.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Lipoyl synthase, mitochondrialUniRule annotation (EC:2.8.1.8UniRule annotation)
    Alternative name(s):
    Lipoate synthaseUniRule annotation
    Short name:
    LSUniRule annotation
    Short name:
    Lip-synUniRule annotation
    Short name:
    mLIP1
    Lipoic acid synthaseUniRule annotation
    Gene namesi
    Name:Lias
    Synonyms:Lip1
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 5

    Organism-specific databases

    MGIiMGI:1934604. Lias.

    Subcellular locationi

    Mitochondrion 1 PublicationUniRule annotation

    GO - Cellular componenti

    1. mitochondrion Source: MGI

    Keywords - Cellular componenti

    Mitochondrion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 2626MitochondrionUniRule annotationAdd
    BLAST
    Chaini27 – 373347Lipoyl synthase, mitochondrialPRO_0000017724Add
    BLAST

    Proteomic databases

    PaxDbiQ99M04.
    PRIDEiQ99M04.

    Expressioni

    Tissue specificityi

    Expressed predominantly in heart, testis, and liver.1 Publication

    Gene expression databases

    ArrayExpressiQ99M04.
    BgeeiQ99M04.
    CleanExiMM_LIAS.
    GenevestigatoriQ99M04.

    Structurei

    3D structure databases

    ProteinModelPortaliQ99M04.
    SMRiQ99M04. Positions 129-309.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the radical SAM superfamily. Lipoyl synthase family.UniRule annotation

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiCOG0320.
    GeneTreeiENSGT00390000006234.
    HOGENOMiHOG000235998.
    HOVERGENiHBG023328.
    InParanoidiQ99M04.
    KOiK03644.
    OMAiHPHIPTK.
    OrthoDBiEOG7P2XS7.
    PhylomeDBiQ99M04.
    TreeFamiTF300817.

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    HAMAPiMF_00206. Lipoyl_synth.
    InterProiIPR013785. Aldolase_TIM.
    IPR006638. Elp3/MiaB/NifB.
    IPR003698. Lipoyl_synth.
    IPR007197. rSAM.
    [Graphical view]
    PANTHERiPTHR10949. PTHR10949. 1 hit.
    PfamiPF04055. Radical_SAM. 1 hit.
    [Graphical view]
    PIRSFiPIRSF005963. Lipoyl_synth. 1 hit.
    SMARTiSM00729. Elp3. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR00510. lipA. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q99M04-1 [UniParc]FASTAAdd to Basket

    « Hide

    MALRCWDTAR SLGSRIFGRY AFTVRALSSL PDKKKEFLHN GPDLQDFVSG    50
    DLADKSTWDE YKGNLKRQKG ERLRLPPWLK TKIPMGKNYN KLKNTLRNLS 100
    LHTVCEEARC PNIGECWGGG EYATATATIM LMGDTCTRGC RFCSVKTARN 150
    PPPLDANEPD NTAKAIAEWG LDYVVLTSVD RDDVADGGAE HIAKTVSCLK 200
    ERNPKILVEC LTPDFRGDLR AVEKVALSGL DVYAHNVETV PELQRKVRDP 250
    RANFDQSLRV LRHAKEVQPD VVSKTSIMLG LGETDEQVYA TLKALRAADV 300
    DCLTLGQYMQ PTKRHLKVEE YVTPEKFKYW EKVGNELGFL YTASGPLVRS 350
    SYKAGEFFLK NLVARRKTKA SKV 373
    Length:373
    Mass (Da):41,879
    Last modified:June 1, 2001 - v1
    Checksum:i6796340A54BC5B8E
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB057731 mRNA. Translation: BAB62009.1.
    AK048744 mRNA. Translation: BAC33443.1.
    AK049548 mRNA. Translation: BAC33804.1.
    AK077192 mRNA. Translation: BAC36672.1.
    BC002141 mRNA. Translation: AAH02141.1.
    CCDSiCCDS19307.1.
    RefSeqiNP_077791.1. NM_024471.4.
    UniGeneiMm.195776.

    Genome annotation databases

    EnsembliENSMUST00000031101; ENSMUSP00000031101; ENSMUSG00000029199.
    GeneIDi79464.
    KEGGimmu:79464.
    UCSCiuc008xnq.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB057731 mRNA. Translation: BAB62009.1 .
    AK048744 mRNA. Translation: BAC33443.1 .
    AK049548 mRNA. Translation: BAC33804.1 .
    AK077192 mRNA. Translation: BAC36672.1 .
    BC002141 mRNA. Translation: AAH02141.1 .
    CCDSi CCDS19307.1.
    RefSeqi NP_077791.1. NM_024471.4.
    UniGenei Mm.195776.

    3D structure databases

    ProteinModelPortali Q99M04.
    SMRi Q99M04. Positions 129-309.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PaxDbi Q99M04.
    PRIDEi Q99M04.

    Protocols and materials databases

    DNASUi 79464.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000031101 ; ENSMUSP00000031101 ; ENSMUSG00000029199 .
    GeneIDi 79464.
    KEGGi mmu:79464.
    UCSCi uc008xnq.1. mouse.

    Organism-specific databases

    CTDi 11019.
    MGIi MGI:1934604. Lias.

    Phylogenomic databases

    eggNOGi COG0320.
    GeneTreei ENSGT00390000006234.
    HOGENOMi HOG000235998.
    HOVERGENi HBG023328.
    InParanoidi Q99M04.
    KOi K03644.
    OMAi HPHIPTK.
    OrthoDBi EOG7P2XS7.
    PhylomeDBi Q99M04.
    TreeFami TF300817.

    Enzyme and pathway databases

    UniPathwayi UPA00538 ; UER00593 .

    Miscellaneous databases

    NextBioi 349935.
    PROi Q99M04.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q99M04.
    Bgeei Q99M04.
    CleanExi MM_LIAS.
    Genevestigatori Q99M04.

    Family and domain databases

    Gene3Di 3.20.20.70. 1 hit.
    HAMAPi MF_00206. Lipoyl_synth.
    InterProi IPR013785. Aldolase_TIM.
    IPR006638. Elp3/MiaB/NifB.
    IPR003698. Lipoyl_synth.
    IPR007197. rSAM.
    [Graphical view ]
    PANTHERi PTHR10949. PTHR10949. 1 hit.
    Pfami PF04055. Radical_SAM. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF005963. Lipoyl_synth. 1 hit.
    SMARTi SM00729. Elp3. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR00510. lipA. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Do mammalian cells synthesize lipoic acid? Identification of a mouse cDNA encoding a lipoic acid synthase located in mitochondria."
      Morikawa T., Yasuno R., Wada H.
      FEBS Lett. 498:16-21(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
      Tissue: Heart.
    2. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
      Tissue: Cerebellum and Testis.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].

    Entry informationi

    Entry nameiLIAS_MOUSE
    AccessioniPrimary (citable) accession number: Q99M04
    Secondary accession number(s): Q543M1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 10, 2002
    Last sequence update: June 1, 2001
    Last modified: October 1, 2014
    This is version 109 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3