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Q99M02

- MARH2_MOUSE

UniProt

Q99M02 - MARH2_MOUSE

Protein

E3 ubiquitin-protein ligase MARCH2

Gene

March2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 112 (01 Oct 2014)
      Sequence version 1 (01 Jun 2001)
      Previous versions | rss
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    Functioni

    E3 ubiquitin-protein ligase that may mediate ubiquitination of TFRC and CD86, and promote their subsequent endocytosis and sorting to lysosomes via multivesicular bodies. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfer the ubiquitin to targeted substrates. May be involved in endosomal trafficking through interaction with STX6 By similarity.By similarity

    Pathwayi

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri56 – 11661RING-CH-typePROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. ligase activity Source: UniProtKB-KW
    2. ubiquitin-protein transferase activity Source: UniProtKB
    3. zinc ion binding Source: InterPro

    GO - Biological processi

    1. endocytosis Source: UniProtKB-KW
    2. protein ubiquitination Source: UniProtKB

    Keywords - Molecular functioni

    Ligase

    Keywords - Biological processi

    Endocytosis, Ubl conjugation pathway

    Keywords - Ligandi

    Metal-binding, Zinc

    Enzyme and pathway databases

    UniPathwayiUPA00143.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    E3 ubiquitin-protein ligase MARCH2 (EC:6.3.2.-)
    Alternative name(s):
    Membrane-associated RING finger protein 2
    Membrane-associated RING-CH protein II
    Short name:
    MARCH-II
    Gene namesi
    Name:March2
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 17

    Organism-specific databases

    MGIiMGI:1925915. March2.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasmic vesicle Source: UniProtKB
    2. endoplasmic reticulum Source: UniProtKB
    3. endoplasmic reticulum membrane Source: UniProtKB-SubCell
    4. endosome membrane Source: UniProtKB-SubCell
    5. integral component of membrane Source: UniProtKB-KW
    6. lysosomal membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Endoplasmic reticulum, Endosome, Lysosome, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 246246E3 ubiquitin-protein ligase MARCH2PRO_0000055926Add
    BLAST

    Proteomic databases

    PRIDEiQ99M02.

    Expressioni

    Gene expression databases

    ArrayExpressiQ99M02.
    BgeeiQ99M02.
    CleanExiMM_MARCH2.
    GenevestigatoriQ99M02.

    Interactioni

    Subunit structurei

    Interacts with STX6 and MARCH3.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliQ99M02.
    SMRiQ99M02. Positions 64-118.
    ModBaseiSearch...
    MobiDBiSearch...

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei138 – 15821HelicalSequence AnalysisAdd
    BLAST
    Transmembranei175 – 19521HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domaini

    The RING-CH-type zinc finger domain is required for E3 ligase activity.PROSITE-ProRule annotation

    Sequence similaritiesi

    Contains 1 RING-CH-type zinc finger.PROSITE-ProRule annotation

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri56 – 11661RING-CH-typePROSITE-ProRule annotationAdd
    BLAST

    Keywords - Domaini

    Transmembrane, Transmembrane helix, Zinc-finger

    Phylogenomic databases

    eggNOGiCOG5183.
    GeneTreeiENSGT00730000110355.
    HOVERGENiHBG052411.
    InParanoidiQ99M02.
    KOiK10657.
    PhylomeDBiQ99M02.

    Family and domain databases

    Gene3Di3.30.40.10. 1 hit.
    InterProiIPR011016. Znf_RING-CH.
    IPR013083. Znf_RING/FYVE/PHD.
    [Graphical view]
    PfamiPF12906. RINGv. 1 hit.
    [Graphical view]
    SMARTiSM00744. RINGv. 1 hit.
    [Graphical view]
    PROSITEiPS51292. ZF_RING_CH. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q99M02-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTTGDCCHLP GSLCDCSSSP AFSKVVEATG LGPPQYVAQV TSRDGRLLST    50
    VIRALDSQSD CPFCRICHEG ANGENLLSPC GCTGTLGAVH KSCLEKWLSS 100
    SNTSYCELCH TEFAVEKRPR PLTEWLKDPG PRTEKRTLCC DMVCFVFITP 150
    LAAISGWLCL RGAQDHLRLH SRLEAVGLIA LTIALFTIYV LWTLVSFRYH 200
    CQLYSEWRKT NQKVRLKIRE ADGSEDPHHS LLATGLLKKV AEETPV 246
    Length:246
    Mass (Da):27,186
    Last modified:June 1, 2001 - v1
    Checksum:iDC4A6EE34B8AE897
    GO

    Sequence cautioni

    The sequence BAC38235.1 differs from that shown. Reason: Frameshift at position 172.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK079234 mRNA. Translation: BAC37584.1.
    AK081495 mRNA. Translation: BAC38235.1. Frameshift.
    BC002144 mRNA. Translation: AAH02144.1.
    CCDSiCCDS57064.1.
    RefSeqiNP_001239409.1. NM_001252480.1.
    UniGeneiMm.311439.
    Mm.374865.

    Genome annotation databases

    EnsembliENSMUST00000172767; ENSMUSP00000134220; ENSMUSG00000079557.
    GeneIDi224703.
    KEGGimmu:224703.
    UCSCiuc008bzk.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK079234 mRNA. Translation: BAC37584.1 .
    AK081495 mRNA. Translation: BAC38235.1 . Frameshift.
    BC002144 mRNA. Translation: AAH02144.1 .
    CCDSi CCDS57064.1.
    RefSeqi NP_001239409.1. NM_001252480.1.
    UniGenei Mm.311439.
    Mm.374865.

    3D structure databases

    ProteinModelPortali Q99M02.
    SMRi Q99M02. Positions 64-118.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PRIDEi Q99M02.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000172767 ; ENSMUSP00000134220 ; ENSMUSG00000079557 .
    GeneIDi 224703.
    KEGGi mmu:224703.
    UCSCi uc008bzk.2. mouse.

    Organism-specific databases

    CTDi 51257.
    MGIi MGI:1925915. March2.

    Phylogenomic databases

    eggNOGi COG5183.
    GeneTreei ENSGT00730000110355.
    HOVERGENi HBG052411.
    InParanoidi Q99M02.
    KOi K10657.
    PhylomeDBi Q99M02.

    Enzyme and pathway databases

    UniPathwayi UPA00143 .

    Miscellaneous databases

    ChiTaRSi MARCH2. mouse.
    NextBioi 377316.
    PROi Q99M02.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q99M02.
    Bgeei Q99M02.
    CleanExi MM_MARCH2.
    Genevestigatori Q99M02.

    Family and domain databases

    Gene3Di 3.30.40.10. 1 hit.
    InterProi IPR011016. Znf_RING-CH.
    IPR013083. Znf_RING/FYVE/PHD.
    [Graphical view ]
    Pfami PF12906. RINGv. 1 hit.
    [Graphical view ]
    SMARTi SM00744. RINGv. 1 hit.
    [Graphical view ]
    PROSITEi PS51292. ZF_RING_CH. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
      Tissue: Head and Urinary bladder.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: FVB/N.
      Tissue: Mammary tumor.

    Entry informationi

    Entry nameiMARH2_MOUSE
    AccessioniPrimary (citable) accession number: Q99M02
    Secondary accession number(s): Q8C4Q5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 4, 2005
    Last sequence update: June 1, 2001
    Last modified: October 1, 2014
    This is version 112 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3