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Protein

Phenylalanine--tRNA ligase, mitochondrial

Gene

Fars2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Is responsible for the charging of tRNA(Phe) with phenylalanine in mitochondrial translation. To a lesser extent, also catalyzes direct attachment of m-Tyr (an oxidized version of Phe) to tRNA(Phe), thereby opening the way for delivery of the misacylated tRNA to the ribosome and incorporation of ROS-damaged amino acid into proteins (By similarity).By similarity

Catalytic activityi

ATP + L-phenylalanine + tRNA(Phe) = AMP + diphosphate + L-phenylalanyl-tRNA(Phe).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei179SubstrateBy similarity1
Binding sitei287Substrate; via carbonyl oxygenBy similarity1
Binding sitei312Substrate; via carbonyl oxygenBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Phenylalanine--tRNA ligase, mitochondrial (EC:6.1.1.20By similarity)
Alternative name(s):
Phenylalanyl-tRNA synthetase
Short name:
PheRS
Gene namesi
Name:Fars2
Synonyms:Fars1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 13

Organism-specific databases

MGIiMGI:1917205 Fars2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_0000035814? – 451Phenylalanine--tRNA ligase, mitochondrial
Transit peptidei1 – ?MitochondrionSequence analysis

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei202N6-acetyllysineBy similarity1

Keywords - PTMi

Acetylation

Proteomic databases

EPDiQ99M01
MaxQBiQ99M01
PaxDbiQ99M01
PeptideAtlasiQ99M01
PRIDEiQ99M01

PTM databases

PhosphoSitePlusiQ99M01

Expressioni

Gene expression databases

BgeeiENSMUSG00000021420
CleanExiMM_FARS2
ExpressionAtlasiQ99M01 baseline and differential
GenevisibleiQ99M01 MM

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000021857

Structurei

3D structure databases

ProteinModelPortaliQ99M01
SMRiQ99M01
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini358 – 450FDX-ACBPROSITE-ProRule annotationAdd BLAST93

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni157 – 160Substrate bindingBy similarity4
Regioni186 – 188Substrate bindingBy similarity3
Regioni193 – 195Substrate bindingBy similarity3

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG2783 Eukaryota
COG0016 LUCA
COG0072 LUCA
GeneTreeiENSGT00530000063467
HOGENOMiHOG000165163
HOVERGENiHBG082540
InParanoidiQ99M01
KOiK01889
OMAiTYFPFTQ
OrthoDBiEOG091G07OD
PhylomeDBiQ99M01
TreeFamiTF105798

Family and domain databases

Gene3Di3.30.70.380, 1 hit
InterProiView protein in InterPro
IPR006195 aa-tRNA-synth_II
IPR005121 Fdx_antiC-bd
IPR036690 Fdx_antiC-bd_sf
IPR004530 Phe-tRNA-synth_IIc_mito
IPR002319 Phenylalanyl-tRNA_Synthase
PANTHERiPTHR11538:SF41 PTHR11538:SF41, 1 hit
PfamiView protein in Pfam
PF03147 FDX-ACB, 1 hit
PF01409 tRNA-synt_2d, 2 hits
SMARTiView protein in SMART
SM00896 FDX-ACB, 1 hit
SUPFAMiSSF54991 SSF54991, 1 hit
TIGRFAMsiTIGR00469 pheS_mito, 1 hit
PROSITEiView protein in PROSITE
PS50862 AA_TRNA_LIGASE_II, 1 hit
PS51447 FDX_ACB, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q99M01-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVCLALVRAA YEHIYLVRKV SHACRCHQHR AWSSKPAASQ SAVQGAPGSV
60 70 80 90 100
LEILGKSYPQ DDHTNLTQKV LSKVGRNLHN QKFHPLWLIK ERVKEHFYQQ
110 120 130 140 150
YMVRSRTPLF SVYDQLPPVV TTWQNFDSLL IPADHPSRKK GDNYYLNRAH
160 170 180 190 200
MLRAHTSAHQ WDLLHAGLNA FLVVGDVYRR DQIDCQHYPV FHQLEGVRLF
210 220 230 240 250
SKHELFAGVK DGESLQLFEE GSRSAHKQET HTMEAVKLVE FDLKQVLTRL
260 270 280 290 300
VTHLFGDGLE VRWVDCYFPF THPSFEMEIN FRGEWLEVLG CGVMEQQLVN
310 320 330 340 350
SAGAQDRIGW AFGLGLERLA MVLYDIPDIR LFWSEDERFL KQFLLSDINQ
360 370 380 390 400
SVKFQPLSKY PAVFNDISFW LPSENYTEND FYDIVRTVGG DLVEKVDLID
410 420 430 440 450
KFEHPKTHRT SHCYRITYRH MERTLSQREV GNVHQAVQEA AVQLLGVEGR

F
Length:451
Mass (Da):52,337
Last modified:June 1, 2001 - v1
Checksum:i79F3C58E77BC4AF5
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti253H → N in BAB28715 (PubMed:16141072).Curated1
Sequence conflicti394E → G in BAB28715 (PubMed:16141072).Curated1
Sequence conflicti403E → K in BAB28715 (PubMed:16141072).Curated1
Sequence conflicti413C → L in BAB28715 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK013211 mRNA Translation: BAB28715.1
AK170986 mRNA Translation: BAE42160.1
BC002147 mRNA Translation: AAH02147.1
CCDSiCCDS26453.1
RefSeqiNP_077236.1, NM_024274.3
XP_006516832.1, XM_006516769.3
XP_011242647.1, XM_011244345.2
UniGeneiMm.70690

Genome annotation databases

EnsembliENSMUST00000021857; ENSMUSP00000021857; ENSMUSG00000021420
ENSMUST00000224241; ENSMUSP00000153628; ENSMUSG00000021420
ENSMUST00000224611; ENSMUSP00000153658; ENSMUSG00000021420
GeneIDi69955
KEGGimmu:69955
UCSCiuc007qck.2 mouse

Similar proteinsi

Entry informationi

Entry nameiSYFM_MOUSE
AccessioniPrimary (citable) accession number: Q99M01
Secondary accession number(s): Q3TBZ7, Q9CYY0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 23, 2004
Last sequence update: June 1, 2001
Last modified: March 28, 2018
This is version 138 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health