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Protein

Phenylalanine--tRNA ligase, mitochondrial

Gene

Fars2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Is responsible for the charging of tRNA(Phe) with phenylalanine in mitochondrial translation. To a lesser extent, also catalyzes direct attachment of m-Tyr (an oxidized version of Phe) to tRNA(Phe), thereby opening the way for delivery of the misacylated tRNA to the ribosome and incorporation of ROS-damaged amino acid into proteins (By similarity).By similarity

Catalytic activityi

ATP + L-phenylalanine + tRNA(Phe) = AMP + diphosphate + L-phenylalanyl-tRNA(Phe).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei179 – 1791SubstrateBy similarity
Binding sitei287 – 2871Substrate; via carbonyl oxygenBy similarity
Binding sitei312 – 3121Substrate; via carbonyl oxygenBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Phenylalanine--tRNA ligase, mitochondrial (EC:6.1.1.20)
Alternative name(s):
Phenylalanyl-tRNA synthetase
Short name:
PheRS
Gene namesi
Name:Fars2
Synonyms:Fars1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 13

Organism-specific databases

MGIiMGI:1917205. Fars2.

Subcellular locationi

GO - Cellular componenti

  • mitochondrial matrix Source: UniProtKB-SubCell
  • mitochondrion Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini? – 451Phenylalanine--tRNA ligase, mitochondrialPRO_0000035814
Transit peptidei1 – ?MitochondrionSequence analysis

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei202 – 2021N6-acetyllysineBy similarity

Keywords - PTMi

Acetylation

Proteomic databases

EPDiQ99M01.
MaxQBiQ99M01.
PaxDbiQ99M01.
PeptideAtlasiQ99M01.
PRIDEiQ99M01.

PTM databases

PhosphoSiteiQ99M01.

Expressioni

Gene expression databases

BgeeiENSMUSG00000021420.
CleanExiMM_FARS2.
ExpressionAtlasiQ99M01. baseline and differential.
GenevisibleiQ99M01. MM.

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000021857.

Structurei

3D structure databases

ProteinModelPortaliQ99M01.
SMRiQ99M01. Positions 47-451.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini358 – 45093FDX-ACBPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni157 – 1604Substrate bindingBy similarity
Regioni186 – 1883Substrate bindingBy similarity
Regioni193 – 1953Substrate bindingBy similarity

Sequence similaritiesi

Contains 1 FDX-ACB domain.PROSITE-ProRule annotation

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG2783. Eukaryota.
COG0016. LUCA.
COG0072. LUCA.
GeneTreeiENSGT00530000063467.
HOGENOMiHOG000165163.
HOVERGENiHBG082540.
InParanoidiQ99M01.
KOiK01889.
OMAiTYFPFTQ.
OrthoDBiEOG091G07OD.
PhylomeDBiQ99M01.
TreeFamiTF105798.

Family and domain databases

Gene3Di3.30.70.380. 1 hit.
InterProiIPR006195. aa-tRNA-synth_II.
IPR004530. Phe-tRNA-synth_IIc_mito.
IPR002319. Phenylalanyl-tRNA_Synthase.
IPR005121. PheS_beta_Fdx_antiC-bd.
[Graphical view]
PfamiPF03147. FDX-ACB. 1 hit.
PF01409. tRNA-synt_2d. 2 hits.
[Graphical view]
SMARTiSM00896. FDX-ACB. 1 hit.
[Graphical view]
SUPFAMiSSF54991. SSF54991. 1 hit.
TIGRFAMsiTIGR00469. pheS_mito. 1 hit.
PROSITEiPS50862. AA_TRNA_LIGASE_II. 1 hit.
PS51447. FDX_ACB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q99M01-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVCLALVRAA YEHIYLVRKV SHACRCHQHR AWSSKPAASQ SAVQGAPGSV
60 70 80 90 100
LEILGKSYPQ DDHTNLTQKV LSKVGRNLHN QKFHPLWLIK ERVKEHFYQQ
110 120 130 140 150
YMVRSRTPLF SVYDQLPPVV TTWQNFDSLL IPADHPSRKK GDNYYLNRAH
160 170 180 190 200
MLRAHTSAHQ WDLLHAGLNA FLVVGDVYRR DQIDCQHYPV FHQLEGVRLF
210 220 230 240 250
SKHELFAGVK DGESLQLFEE GSRSAHKQET HTMEAVKLVE FDLKQVLTRL
260 270 280 290 300
VTHLFGDGLE VRWVDCYFPF THPSFEMEIN FRGEWLEVLG CGVMEQQLVN
310 320 330 340 350
SAGAQDRIGW AFGLGLERLA MVLYDIPDIR LFWSEDERFL KQFLLSDINQ
360 370 380 390 400
SVKFQPLSKY PAVFNDISFW LPSENYTEND FYDIVRTVGG DLVEKVDLID
410 420 430 440 450
KFEHPKTHRT SHCYRITYRH MERTLSQREV GNVHQAVQEA AVQLLGVEGR

F
Length:451
Mass (Da):52,337
Last modified:June 1, 2001 - v1
Checksum:i79F3C58E77BC4AF5
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti253 – 2531H → N in BAB28715 (PubMed:16141072).Curated
Sequence conflicti394 – 3941E → G in BAB28715 (PubMed:16141072).Curated
Sequence conflicti403 – 4031E → K in BAB28715 (PubMed:16141072).Curated
Sequence conflicti413 – 4131C → L in BAB28715 (PubMed:16141072).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK013211 mRNA. Translation: BAB28715.1.
AK170986 mRNA. Translation: BAE42160.1.
BC002147 mRNA. Translation: AAH02147.1.
CCDSiCCDS26453.1.
RefSeqiNP_077236.1. NM_024274.3.
XP_006516832.1. XM_006516769.2.
XP_011242647.1. XM_011244345.1.
UniGeneiMm.70690.

Genome annotation databases

EnsembliENSMUST00000021857; ENSMUSP00000021857; ENSMUSG00000021420.
GeneIDi69955.
KEGGimmu:69955.
UCSCiuc007qck.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK013211 mRNA. Translation: BAB28715.1.
AK170986 mRNA. Translation: BAE42160.1.
BC002147 mRNA. Translation: AAH02147.1.
CCDSiCCDS26453.1.
RefSeqiNP_077236.1. NM_024274.3.
XP_006516832.1. XM_006516769.2.
XP_011242647.1. XM_011244345.1.
UniGeneiMm.70690.

3D structure databases

ProteinModelPortaliQ99M01.
SMRiQ99M01. Positions 47-451.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000021857.

PTM databases

PhosphoSiteiQ99M01.

Proteomic databases

EPDiQ99M01.
MaxQBiQ99M01.
PaxDbiQ99M01.
PeptideAtlasiQ99M01.
PRIDEiQ99M01.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000021857; ENSMUSP00000021857; ENSMUSG00000021420.
GeneIDi69955.
KEGGimmu:69955.
UCSCiuc007qck.2. mouse.

Organism-specific databases

CTDi10667.
MGIiMGI:1917205. Fars2.

Phylogenomic databases

eggNOGiKOG2783. Eukaryota.
COG0016. LUCA.
COG0072. LUCA.
GeneTreeiENSGT00530000063467.
HOGENOMiHOG000165163.
HOVERGENiHBG082540.
InParanoidiQ99M01.
KOiK01889.
OMAiTYFPFTQ.
OrthoDBiEOG091G07OD.
PhylomeDBiQ99M01.
TreeFamiTF105798.

Miscellaneous databases

ChiTaRSiFars2. mouse.
PROiQ99M01.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000021420.
CleanExiMM_FARS2.
ExpressionAtlasiQ99M01. baseline and differential.
GenevisibleiQ99M01. MM.

Family and domain databases

Gene3Di3.30.70.380. 1 hit.
InterProiIPR006195. aa-tRNA-synth_II.
IPR004530. Phe-tRNA-synth_IIc_mito.
IPR002319. Phenylalanyl-tRNA_Synthase.
IPR005121. PheS_beta_Fdx_antiC-bd.
[Graphical view]
PfamiPF03147. FDX-ACB. 1 hit.
PF01409. tRNA-synt_2d. 2 hits.
[Graphical view]
SMARTiSM00896. FDX-ACB. 1 hit.
[Graphical view]
SUPFAMiSSF54991. SSF54991. 1 hit.
TIGRFAMsiTIGR00469. pheS_mito. 1 hit.
PROSITEiPS50862. AA_TRNA_LIGASE_II. 1 hit.
PS51447. FDX_ACB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSYFM_MOUSE
AccessioniPrimary (citable) accession number: Q99M01
Secondary accession number(s): Q3TBZ7, Q9CYY0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 23, 2004
Last sequence update: June 1, 2001
Last modified: September 7, 2016
This is version 126 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.