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Protein

DNA replication complex GINS protein SLD5

Gene

Gins4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

The GINS complex plays an essential role in the initiation of DNA replication, and progression of DNA replication forks. GINS4 is important for GINS complex assembly. GINS complex seems to bind preferentially to single-stranded DNA (By similarity).By similarity

GO - Biological processi

  • DNA replication Source: UniProtKB-KW
  • inner cell mass cell proliferation Source: MGI
Complete GO annotation...

Keywords - Biological processi

DNA replication

Enzyme and pathway databases

ReactomeiR-MMU-176974. Unwinding of DNA.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA replication complex GINS protein SLD5
Alternative name(s):
GINS complex subunit 4
Cleaved into the following chain:
Gene namesi
Name:Gins4
Synonyms:Sld5
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 8

Organism-specific databases

MGIiMGI:1923847. Gins4.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: MGI
  • nucleus Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 223223DNA replication complex GINS protein SLD5PRO_0000327621Add
BLAST
Initiator methionineiRemoved; alternateBy similarity
Chaini2 – 223222DNA replication complex GINS protein SLD5, N-terminally processedPRO_0000421795Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionineBy similarity
Modified residuei2 – 21N-acetylthreonine; in DNA replication complex GINS protein SLD5, N-terminally processedBy similarity
Modified residuei12 – 121PhosphoserineBy similarity
Modified residuei16 – 161PhosphoserineBy similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ99LZ3.
MaxQBiQ99LZ3.
PaxDbiQ99LZ3.
PRIDEiQ99LZ3.

PTM databases

PhosphoSiteiQ99LZ3.

Expressioni

Tissue specificityi

Highly abundant in testis. Weakly expressed in thymus and bone marrow.1 Publication

Gene expression databases

BgeeiQ99LZ3.
GenevisibleiQ99LZ3. MM.

Interactioni

Subunit structurei

Component of the GINS complex which is a heterotetramer of GINS1, GINS2, GINS3 and GINS4. Forms a stable subcomplex with GINS1. GINS complex interacts with DNA primase in vitro (By similarity).By similarity

Protein-protein interaction databases

BioGridi224572. 1 interaction.
IntActiQ99LZ3. 1 interaction.
MINTiMINT-4612415.
STRINGi10090.ENSMUSP00000033950.

Structurei

3D structure databases

ProteinModelPortaliQ99LZ3.
SMRiQ99LZ3. Positions 21-212.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni166 – 22358Important for GINS complex assemblyBy similarityAdd
BLAST

Sequence similaritiesi

Belongs to the GINS4/SLD5 family.Curated

Phylogenomic databases

eggNOGiKOG3176. Eukaryota.
COG5086. LUCA.
GeneTreeiENSGT00390000003246.
HOGENOMiHOG000215319.
HOVERGENiHBG059490.
InParanoidiQ99LZ3.
KOiK10735.
OMAiVSIHRME.
OrthoDBiEOG7ZSHV8.
PhylomeDBiQ99LZ3.
TreeFamiTF105863.

Family and domain databases

InterProiIPR021151. GINS_complex.
IPR008591. GINS_Sld5.
IPR031633. SLD5_C.
[Graphical view]
PANTHERiPTHR21206. PTHR21206. 1 hit.
PfamiPF05916. Sld5. 1 hit.
PF16922. SLD5_C. 1 hit.
[Graphical view]
PIRSFiPIRSF007764. Sld5. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q99LZ3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTEVLDLHGQ DSDGGSEEMV LTPAELIEKL EQAWMNEKFA PELLESKAEI
60 70 80 90 100
VECVMEQLEH MEENLRRAKK GDLKVSIHRM EMERIRYVLS SYLRCRLMKI
110 120 130 140 150
EKFFPHILEK EKVRSEGEPS SLSPEEFVFA KEYMDHTETH FKNVALKHMP
160 170 180 190 200
PNLQKVDLLR AVPKPDLDSY VFLRVKERQE NILVEPEADE QRDYVIDLEV
210 220
GSQHLIRYKT IAPLVASGAV QLI
Length:223
Mass (Da):25,961
Last modified:June 1, 2001 - v1
Checksum:iA6E1B88E95F6DF1F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK076104 mRNA. Translation: BAC36185.1.
AK164815 mRNA. Translation: BAE37931.1.
BC002156 mRNA. Translation: AAH02156.1.
CCDSiCCDS22190.1.
RefSeqiNP_077202.1. NM_024240.6.
UniGeneiMm.27945.

Genome annotation databases

EnsembliENSMUST00000033950; ENSMUSP00000033950; ENSMUSG00000031546.
GeneIDi109145.
KEGGimmu:109145.
UCSCiuc009ler.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK076104 mRNA. Translation: BAC36185.1.
AK164815 mRNA. Translation: BAE37931.1.
BC002156 mRNA. Translation: AAH02156.1.
CCDSiCCDS22190.1.
RefSeqiNP_077202.1. NM_024240.6.
UniGeneiMm.27945.

3D structure databases

ProteinModelPortaliQ99LZ3.
SMRiQ99LZ3. Positions 21-212.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi224572. 1 interaction.
IntActiQ99LZ3. 1 interaction.
MINTiMINT-4612415.
STRINGi10090.ENSMUSP00000033950.

PTM databases

PhosphoSiteiQ99LZ3.

Proteomic databases

EPDiQ99LZ3.
MaxQBiQ99LZ3.
PaxDbiQ99LZ3.
PRIDEiQ99LZ3.

Protocols and materials databases

DNASUi109145.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000033950; ENSMUSP00000033950; ENSMUSG00000031546.
GeneIDi109145.
KEGGimmu:109145.
UCSCiuc009ler.2. mouse.

Organism-specific databases

CTDi84296.
MGIiMGI:1923847. Gins4.

Phylogenomic databases

eggNOGiKOG3176. Eukaryota.
COG5086. LUCA.
GeneTreeiENSGT00390000003246.
HOGENOMiHOG000215319.
HOVERGENiHBG059490.
InParanoidiQ99LZ3.
KOiK10735.
OMAiVSIHRME.
OrthoDBiEOG7ZSHV8.
PhylomeDBiQ99LZ3.
TreeFamiTF105863.

Enzyme and pathway databases

ReactomeiR-MMU-176974. Unwinding of DNA.

Miscellaneous databases

PROiQ99LZ3.
SOURCEiSearch...

Gene expression databases

BgeeiQ99LZ3.
GenevisibleiQ99LZ3. MM.

Family and domain databases

InterProiIPR021151. GINS_complex.
IPR008591. GINS_Sld5.
IPR031633. SLD5_C.
[Graphical view]
PANTHERiPTHR21206. PTHR21206. 1 hit.
PfamiPF05916. Sld5. 1 hit.
PF16922. SLD5_C. 1 hit.
[Graphical view]
PIRSFiPIRSF007764. Sld5. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Heart.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Mammary tumor.
  3. "Identification and characterization of mouse PSF1-binding protein, SLD5."
    Kong L., Ueno M., Itoh M., Yoshioka K., Takakura N.
    Biochem. Biophys. Res. Commun. 339:1204-1207(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH GINS1, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
  4. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Spleen and Testis.

Entry informationi

Entry nameiSLD5_MOUSE
AccessioniPrimary (citable) accession number: Q99LZ3
Secondary accession number(s): Q3TP06
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: June 1, 2001
Last modified: June 8, 2016
This is version 105 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.