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Protein

YY1-associated factor 2

Gene

Yaf2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Binds to MYC and inhibits MYC-mediated transactivation. Also binds to MYCN and enhances MYCN-dependent transcriptional activation. Increases calpain 2-mediated proteolysis of YY1 in vitro. Component of the E2F6.com-1 complex, a repressive complex that methylates 'Lys-9' of histone H3, suggesting that it is involved in chromatin-remodeling.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri19 – 4830RanBP2-typePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
YY1-associated factor 2
Gene namesi
Name:Yaf2
OrganismiMus musculus (Mouse)Imported
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 15

Organism-specific databases

MGIiMGI:1914307. Yaf2.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: Ensembl
  • nucleoplasm Source: Ensembl
  • nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 179179YY1-associated factor 2PRO_0000066114Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei166 – 1661PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ99LW6.
MaxQBiQ99LW6.
PaxDbiQ99LW6.
PeptideAtlasiQ99LW6.
PRIDEiQ99LW6.

PTM databases

iPTMnetiQ99LW6.
PhosphoSiteiQ99LW6.

Expressioni

Tissue specificityi

In the mesoderm, expressed in the region close to the surface ectoderm.1 Publication

Developmental stagei

Expressed in both pre- and post-implantation embryos.1 Publication

Gene expression databases

BgeeiQ99LW6.
CleanExiMM_YAF2.
GenevisibleiQ99LW6. MM.

Interactioni

Subunit structurei

Interacts with MYC, MYCN, RNF2/RING1B and YY1. Part of the E2F6.com-1 complex in G0 phase composed of E2F6, MGA, MAX, TFDP1, CBX3, BAT8, EUHMTASE1, RING1, RNF2, MBLR, L3MBTL2 and YAF2.1 Publication

Protein-protein interaction databases

BioGridi211908. 2 interactions.
IntActiQ99LW6. 3 interactions.
STRINGi10090.ENSMUSP00000079179.

Structurei

3D structure databases

ProteinModelPortaliQ99LW6.
SMRiQ99LW6. Positions 19-58, 101-133.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Contains 1 RanBP2-type zinc finger.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri19 – 4830RanBP2-typePROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiKOG4477. Eukaryota.
ENOG4111PN1. LUCA.
GeneTreeiENSGT00390000013995.
HOGENOMiHOG000007553.
HOVERGENiHBG001768.
InParanoidiQ99LW6.
KOiK11468.
OMAiVNQQFAS.
OrthoDBiEOG7B8S61.
PhylomeDBiQ99LW6.
TreeFamiTF350501.

Family and domain databases

InterProiIPR001876. Znf_RanBP2.
[Graphical view]
PfamiPF00641. zf-RanBP. 1 hit.
[Graphical view]
SMARTiSM00547. ZnF_RBZ. 1 hit.
[Graphical view]
SUPFAMiSSF90209. SSF90209. 1 hit.
PROSITEiPS01358. ZF_RANBP2_1. 1 hit.
PS50199. ZF_RANBP2_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 11 Publication (identifier: Q99LW6-1) [UniParc]FASTAAdd to basket

Also known as: YAF2-a1 Publication

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGDKKSPTRP KRQPKPASDE GYWDCSVCTF RNSAEAFKCM MCDVRKGTST
60 70 80 90 100
RKPRPVSQLV AQQVTQQFVP PTQSKKEKKD RVEKDKSEKE AASKKNCHKK
110 120 130 140 150
TRPRLKNVDR SSAQHLEVTV GDLTVIITDF KEKAKSAPAS SAAGDQHSQG
160 170
SCSSDSTERG VSRSSSPRGE ASSLNGESH
Length:179
Mass (Da):19,655
Last modified:June 1, 2001 - v1
Checksum:i7DDB7B7F171CB547
GO
Isoform 21 Publication (identifier: Q99LW6-2) [UniParc]FASTAAdd to basket

Also known as: YAF2-b1 Publication

The sequence of this isoform differs from the canonical sequence as follows:
     52-68: KPRPVSQLVAQQVTQQF → STFSEAIANALRTKGPL
     69-179: Missing.

Show »
Length:68
Mass (Da):7,548
Checksum:i1E9AB907CD05D5C2
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti98 – 981H → S in BAB23741 (PubMed:16141072).Curated
Sequence conflicti107 – 1071N → Y in BAB23741 (PubMed:16141072).Curated
Sequence conflicti162 – 1621S → F in BAB23741 (PubMed:16141072).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei52 – 6817KPRPV…VTQQF → STFSEAIANALRTKGPL in isoform 2. 1 PublicationVSP_050619Add
BLAST
Alternative sequencei69 – 179111Missing in isoform 2. 1 PublicationVSP_050620Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB100452 mRNA. Translation: BAC97817.1.
AB100453 mRNA. Translation: BAC97818.1.
AK005008 mRNA. Translation: BAB23741.1.
AK148634 mRNA. Translation: BAE28627.1.
BC002192 mRNA. Translation: AAH02192.1.
CCDSiCCDS27765.1. [Q99LW6-1]
RefSeqiNP_077151.3. NM_024189.6. [Q99LW6-1]
UniGeneiMm.4714.

Genome annotation databases

EnsembliENSMUST00000080299; ENSMUSP00000079179; ENSMUSG00000022634. [Q99LW6-1]
GeneIDi67057.
KEGGimmu:67057.
UCSCiuc007xis.2. mouse. [Q99LW6-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB100452 mRNA. Translation: BAC97817.1.
AB100453 mRNA. Translation: BAC97818.1.
AK005008 mRNA. Translation: BAB23741.1.
AK148634 mRNA. Translation: BAE28627.1.
BC002192 mRNA. Translation: AAH02192.1.
CCDSiCCDS27765.1. [Q99LW6-1]
RefSeqiNP_077151.3. NM_024189.6. [Q99LW6-1]
UniGeneiMm.4714.

3D structure databases

ProteinModelPortaliQ99LW6.
SMRiQ99LW6. Positions 19-58, 101-133.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi211908. 2 interactions.
IntActiQ99LW6. 3 interactions.
STRINGi10090.ENSMUSP00000079179.

PTM databases

iPTMnetiQ99LW6.
PhosphoSiteiQ99LW6.

Proteomic databases

EPDiQ99LW6.
MaxQBiQ99LW6.
PaxDbiQ99LW6.
PeptideAtlasiQ99LW6.
PRIDEiQ99LW6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000080299; ENSMUSP00000079179; ENSMUSG00000022634. [Q99LW6-1]
GeneIDi67057.
KEGGimmu:67057.
UCSCiuc007xis.2. mouse. [Q99LW6-1]

Organism-specific databases

CTDi10138.
MGIiMGI:1914307. Yaf2.

Phylogenomic databases

eggNOGiKOG4477. Eukaryota.
ENOG4111PN1. LUCA.
GeneTreeiENSGT00390000013995.
HOGENOMiHOG000007553.
HOVERGENiHBG001768.
InParanoidiQ99LW6.
KOiK11468.
OMAiVNQQFAS.
OrthoDBiEOG7B8S61.
PhylomeDBiQ99LW6.
TreeFamiTF350501.

Miscellaneous databases

PROiQ99LW6.
SOURCEiSearch...

Gene expression databases

BgeeiQ99LW6.
CleanExiMM_YAF2.
GenevisibleiQ99LW6. MM.

Family and domain databases

InterProiIPR001876. Znf_RanBP2.
[Graphical view]
PfamiPF00641. zf-RanBP. 1 hit.
[Graphical view]
SMARTiSM00547. ZnF_RBZ. 1 hit.
[Graphical view]
SUPFAMiSSF90209. SSF90209. 1 hit.
PROSITEiPS01358. ZF_RANBP2_1. 1 hit.
PS50199. ZF_RANBP2_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The mouse YAF2 gene generates two distinct transcripts and is expressed in pre- and postimplantation embryos."
    Kaneko T., Miyagishima H., Hasegawa T., Mizutani-Koseki Y., Isono K., Koseki H.
    Gene 315:183-192(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, INTERACTION WITH RNF2.
    Tissue: BrainImported.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: C57BL/6J.
    Tissue: Liver and Sympathetic ganglion.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Mammary gland1 Publication.
  4. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-166, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Heart and Testis.

Entry informationi

Entry nameiYAF2_MOUSE
AccessioniPrimary (citable) accession number: Q99LW6
Secondary accession number(s): Q3UFD4, Q9DBE4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 31, 2003
Last sequence update: June 1, 2001
Last modified: July 6, 2016
This is version 114 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.