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Protein

Phosphatidylinositol-glycan biosynthesis class X protein

Gene

Pigx

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Essential component of glycosylphosphatidylinositol-mannosyltransferase 1 which transfers the first of the 4 mannoses in the GPI-anchor precursors during GPI-anchor biosynthesis. Probably acts by stabilizing the mannosyltransferase PIGM (By similarity).By similarity

Pathwayi: glycosylphosphatidylinositol-anchor biosynthesis

This protein is involved in the pathway glycosylphosphatidylinositol-anchor biosynthesis, which is part of Glycolipid biosynthesis.
View all proteins of this organism that are known to be involved in the pathway glycosylphosphatidylinositol-anchor biosynthesis and in Glycolipid biosynthesis.

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

GPI-anchor biosynthesis

Enzyme and pathway databases

UniPathwayiUPA00196.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphatidylinositol-glycan biosynthesis class X protein
Short name:
PIG-X
Gene namesi
Name:Pigx
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:1919334. Pigx.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini23 – 226204LumenalSequence analysisAdd
BLAST
Transmembranei227 – 24721HelicalSequence analysisAdd
BLAST
Topological domaini248 – 2547CytoplasmicSequence analysis

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2222Sequence analysisAdd
BLAST
Chaini23 – 254232Phosphatidylinositol-glycan biosynthesis class X proteinPRO_0000246296Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi211 – 2111N-linked (GlcNAc...)Sequence analysis

Post-translational modificationi

N-glycosylated.By similarity

Keywords - PTMi

Glycoprotein

Proteomic databases

EPDiQ99LV7.
MaxQBiQ99LV7.
PaxDbiQ99LV7.
PRIDEiQ99LV7.

PTM databases

PhosphoSiteiQ99LV7.

Interactioni

Subunit structurei

Interacts with PIGM.By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000093819.

Family & Domainsi

Sequence similaritiesi

Belongs to the PIGX family.Curated

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IGQT. Eukaryota.
ENOG410Y4AX. LUCA.
HOGENOMiHOG000015227.
HOVERGENiHBG060417.
InParanoidiQ99LV7.
KOiK07541.
PhylomeDBiQ99LV7.

Family and domain databases

InterProiIPR013233. PIG-X/PBN1.
[Graphical view]
PfamiPF08320. PIG-X. 1 hit.
[Graphical view]
SMARTiSM00780. PIG-X. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q99LV7-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAAGAVAWLL LWAAWLVGRL AADFSDAPFS AGKGVRATCS EIILRQEFLK
60 70 80 90 100
DGFHRDLLIK VKFGESIEDL QTCRLLIKHY IPPGLFVDPY ELASLRERNL
110 120 130 140 150
TEAVMLSESF NIEAPNYLSN ESAVLIYARQ DAQCIDCFQA FLPVHYRYHR
160 170 180 190 200
PHKKDGDTLI VVNNPDLLMY CDQEFPILKC WAQSEVAAPC ALKSEEICQW
210 220 230 240 250
KSMQYKSILK NLTVQVPVGL TIHTSLVCSV TLLITILCST LILLAVFKYG

HFSL
Length:254
Mass (Da):28,644
Last modified:July 25, 2006 - v2
Checksum:iB2724144EFAD41D7
GO
Isoform 2 (identifier: Q99LV7-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-104: Missing.

Note: No experimental confirmation available.
Show »
Length:150
Mass (Da):17,031
Checksum:iA0CBE28DC2145BCC
GO

Sequence cautioni

The sequence BAC25223.1 differs from that shown.Unusual initiator. The initiator methionine is coded by a non-canonical CTG leucine codon.Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 104104Missing in isoform 2. 1 PublicationVSP_019843Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK008583 mRNA. Translation: BAC25223.1. Sequence problems.
BC002202 mRNA. Translation: AAH02202.1.
CCDSiCCDS28113.2. [Q99LV7-1]
RefSeqiNP_077784.2. NM_024464.3. [Q99LV7-1]
UniGeneiMm.37613.

Genome annotation databases

GeneIDi72084.
KEGGimmu:72084.
UCSCiuc007yye.3. mouse. [Q99LV7-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK008583 mRNA. Translation: BAC25223.1. Sequence problems.
BC002202 mRNA. Translation: AAH02202.1.
CCDSiCCDS28113.2. [Q99LV7-1]
RefSeqiNP_077784.2. NM_024464.3. [Q99LV7-1]
UniGeneiMm.37613.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000093819.

PTM databases

PhosphoSiteiQ99LV7.

Proteomic databases

EPDiQ99LV7.
MaxQBiQ99LV7.
PaxDbiQ99LV7.
PRIDEiQ99LV7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi72084.
KEGGimmu:72084.
UCSCiuc007yye.3. mouse. [Q99LV7-1]

Organism-specific databases

CTDi54965.
MGIiMGI:1919334. Pigx.

Phylogenomic databases

eggNOGiENOG410IGQT. Eukaryota.
ENOG410Y4AX. LUCA.
HOGENOMiHOG000015227.
HOVERGENiHBG060417.
InParanoidiQ99LV7.
KOiK07541.
PhylomeDBiQ99LV7.

Enzyme and pathway databases

UniPathwayiUPA00196.

Miscellaneous databases

NextBioi335396.
PROiQ99LV7.
SOURCEiSearch...

Family and domain databases

InterProiIPR013233. PIG-X/PBN1.
[Graphical view]
PfamiPF08320. PIG-X. 1 hit.
[Graphical view]
SMARTiSM00780. PIG-X. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: C57BL/6J.
    Tissue: Small intestine.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Strain: FVB/N.
    Tissue: Mammary tumor.
  3. "Mammalian PIG-X and yeast Pbn1p are the essential components of glycosylphosphatidylinositol-mannosyltransferase I."
    Ashida H., Hong Y., Murakami Y., Shishioh N., Sugimoto N., Kim Y.U., Maeda Y., Kinoshita T.
    Mol. Biol. Cell 16:1439-1448(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: INITIATION CODON CTG.

Entry informationi

Entry nameiPIGX_MOUSE
AccessioniPrimary (citable) accession number: Q99LV7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 25, 2006
Last sequence update: July 25, 2006
Last modified: May 11, 2016
This is version 97 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.