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Protein

Phosphoserine phosphatase

Gene

Psph

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the last step in the biosynthesis of serine from carbohydrates. The reaction mechanism proceeds via the formation of a phosphoryl-enzyme intermediates (By similarity).By similarity

Catalytic activityi

O-phospho-L(or D)-serine + H2O = L(or D)-serine + phosphate.

Cofactori

Mg2+By similarityNote: Binds 1 Mg2+ ion per subunit.By similarity

Pathwayi: L-serine biosynthesis

This protein is involved in step 3 of the subpathway that synthesizes L-serine from 3-phospho-D-glycerate.
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. D-3-phosphoglycerate dehydrogenase (Phgdh)
  2. Phosphoserine aminotransferase (Psat1), Phosphoserine aminotransferase (Psat1), Phosphoserine aminotransferase (Psat1), Phosphoserine aminotransferase (Psat1), Phosphoserine aminotransferase (Psat1)
  3. Phosphoserine phosphatase (Psph)
This subpathway is part of the pathway L-serine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-serine from 3-phospho-D-glycerate, the pathway L-serine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei20NucleophileBy similarity1
Metal bindingi20MagnesiumBy similarity1
Active sitei22Proton donorBy similarity1
Metal bindingi22Magnesium; via carbonyl oxygenBy similarity1
Binding sitei29SubstrateBy similarity1
Binding sitei65SubstrateBy similarity1
Binding sitei158SubstrateBy similarity1
Metal bindingi179MagnesiumBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase
Biological processAmino-acid biosynthesis, Serine biosynthesis
LigandMagnesium, Metal-binding

Enzyme and pathway databases

BRENDAi3.1.3.3 3474
ReactomeiR-MMU-977347 Serine biosynthesis
UniPathwayiUPA00135; UER00198

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoserine phosphatase (EC:3.1.3.3)
Short name:
PSP
Short name:
PSPase
Alternative name(s):
O-phosphoserine phosphohydrolase
Gene namesi
Name:Psph
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:97788 Psph

Subcellular locationi

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001568801 – 225Phosphoserine phosphataseAdd BLAST225

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1

Keywords - PTMi

Acetylation

Proteomic databases

EPDiQ99LS3
MaxQBiQ99LS3
PaxDbiQ99LS3
PeptideAtlasiQ99LS3
PRIDEiQ99LS3

2D gel databases

UCD-2DPAGEiQ99LS3

PTM databases

iPTMnetiQ99LS3
PhosphoSitePlusiQ99LS3

Expressioni

Gene expression databases

BgeeiENSMUSG00000029446
CleanExiMM_PSPH
ExpressionAtlasiQ99LS3 baseline and differential
GenevisibleiQ99LS3 MM

Interactioni

Subunit structurei

Homodimer.By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi221509, 1 interactor
IntActiQ99LS3, 2 interactors
MINTiQ99LS3
STRINGi10090.ENSMUSP00000031399

Structurei

3D structure databases

ProteinModelPortaliQ99LS3
SMRiQ99LS3
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni109 – 110Substrate bindingBy similarity2

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG1615 Eukaryota
COG0560 LUCA
GeneTreeiENSGT00390000003115
HOGENOMiHOG000231116
HOVERGENiHBG057486
InParanoidiQ99LS3
KOiK01079
OMAiRAQYYVT
OrthoDBiEOG091G0JBZ
PhylomeDBiQ99LS3
TreeFamiTF315024

Family and domain databases

Gene3Di1.10.150.210, 2 hits
3.40.50.1000, 3 hits
InterProiView protein in InterPro
IPR036412 HAD-like_sf
IPR023214 HAD_sf
IPR023190 Pser_Pase_dom_2
SUPFAMiSSF56784 SSF56784, 1 hit

Sequencei

Sequence statusi: Complete.

Q99LS3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVSHSELRKL FCSADAVCFD VDSTVIREEG IDELAKFCGV EAAVSEMTRR
60 70 80 90 100
AMGGALPFKD ALTQRLALIQ PSRDQVQRLL AEHPPHLTPG IRELVSRLQE
110 120 130 140 150
RNVQVFLISG GFRSIVEHVA AKLNIPTTNV FANRLKFYFN GEYAGFDEMQ
160 170 180 190 200
PTAESGGKGK VIRFLKEKFH FKKIIMIGDG ATDMEACPPA DAFIGFGGNV
210 220
IRQQVKDNAK WYITDFVELL GELEE
Length:225
Mass (Da):25,096
Last modified:June 1, 2001 - v1
Checksum:iCCEE55B48787D10A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK088865 mRNA Translation: BAC40622.1
AK168048 mRNA Translation: BAE40030.1
BC002251 mRNA Translation: AAH02251.1
CCDSiCCDS19699.1
RefSeqiNP_598661.1, NM_133900.4
XP_006504337.1, XM_006504274.3
XP_017176063.1, XM_017320574.1
UniGeneiMm.271784

Genome annotation databases

EnsembliENSMUST00000031399; ENSMUSP00000031399; ENSMUSG00000029446
GeneIDi100678
KEGGimmu:100678
UCSCiuc008zth.2 mouse

Similar proteinsi

Entry informationi

Entry nameiSERB_MOUSE
AccessioniPrimary (citable) accession number: Q99LS3
Secondary accession number(s): Q3TI16, Q5XHW3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 26, 2005
Last sequence update: June 1, 2001
Last modified: April 25, 2018
This is version 130 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

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