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Protein

Monoacylglycerol lipase ABHD12

Gene

Abhd12

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Lysophosphatidylserine (LPS) lipase that plays a key role in the central nervous system. Represents a major LPS lipase in the brain (PubMed:23297193). May also have a 2-arachidonoylglycerol (2-AG) hydrolase activity and act as a regulator of endocannabinoid signaling pathways (PubMed:18096503).2 Publications

Catalytic activityi

Hydrolyzes glycerol monoesters of long-chain fatty acids.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei246 – 2461NucleophileBy similarity
Active sitei333 – 3331Charge relay systemBy similarity
Active sitei372 – 3721Charge relay systemBy similarity

GO - Molecular functioni

  • acylglycerol lipase activity Source: UniProtKB

GO - Biological processi

  • acylglycerol catabolic process Source: UniProtKB
  • adult walking behavior Source: MGI
  • phosphatidylserine catabolic process Source: MGI
  • response to auditory stimulus Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Enzyme and pathway databases

ReactomeiR-MMU-426048. Arachidonate production from DAG.

Protein family/group databases

ESTHERimouse-abd12. AlphaBeta_hydrolase.
MEROPSiS09.054.

Chemistry

SwissLipidsiSLP:000001041.

Names & Taxonomyi

Protein namesi
Recommended name:
Monoacylglycerol lipase ABHD12Curated (EC:3.1.1.231 Publication)
Alternative name(s):
2-arachidonoylglycerol hydrolaseCurated
Abhydrolase domain-containing protein 12Curated
Gene namesi
Name:Abhd12Imported
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:1923442. Abhd12.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 7474Cytoplasmic1 PublicationAdd
BLAST
Transmembranei75 – 9521Helical1 PublicationAdd
BLAST
Topological domaini96 – 398303Extracellular1 PublicationAdd
BLAST

GO - Cellular componenti

  • AMPA glutamate receptor complex Source: MGI
  • integral component of membrane Source: UniProtKB
  • mitochondrion Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion

Pathology & Biotechi

Disruption phenotypei

Mice are viable and were born at the expected Mendelian frequency. Young mice (less than 6 months old) are mostly normal in their behavior; however, as these animals age, they develop an array of phenotypes, including defective auditory and motor behavior, with concomitant cellular pathology indicative of a neuroinflammatory response. Metabolomic characterization of brain tissue show striking elevations in a series of lysophosphatidylserine (LPS) lipids that occur before the onset of neuroinflammatory and behavioral defects.1 Publication

Chemistry

ChEMBLiCHEMBL5972.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 398398Monoacylglycerol lipase ABHD12PRO_0000079414Add
BLAST

Post-translational modificationi

Glycosylated.1 Publication

Keywords - PTMi

Glycoprotein

Proteomic databases

EPDiQ99LR1.
MaxQBiQ99LR1.
PaxDbiQ99LR1.
PRIDEiQ99LR1.

PTM databases

PhosphoSiteiQ99LR1.
SwissPalmiQ99LR1.

Expressioni

Gene expression databases

BgeeiQ99LR1.
CleanExiMM_ABHD12.
ExpressionAtlasiQ99LR1. baseline and differential.
GenevisibleiQ99LR1. MM.

Interactioni

Protein-protein interaction databases

IntActiQ99LR1. 2 interactions.
MINTiMINT-4124321.
STRINGi10090.ENSMUSP00000053558.

Chemistry

BindingDBiQ99LR1.

Structurei

3D structure databases

ProteinModelPortaliQ99LR1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi18 – 258Poly-Ser
Compositional biasi26 – 294Poly-Ala

Sequence similaritiesi

Belongs to the serine esterase family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1552. Eukaryota.
COG1073. LUCA.
GeneTreeiENSGT00390000006625.
HOGENOMiHOG000230822.
HOVERGENiHBG051152.
InParanoidiQ99LR1.
KOiK13704.
OMAiKYPKGLD.
OrthoDBiEOG75TMCD.
PhylomeDBiQ99LR1.
TreeFamiTF315122.

Family and domain databases

Gene3Di3.40.50.1820. 2 hits.
InterProiIPR029058. AB_hydrolase.
IPR026605. ABHD12.
IPR022742. Hydrolase_4.
[Graphical view]
PANTHERiPTHR12277:SF61. PTHR12277:SF61. 1 hit.
PfamiPF12146. Hydrolase_4. 1 hit.
[Graphical view]
SUPFAMiSSF53474. SSF53474. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q99LR1-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MRKRTEPVTL EHERCAASGS SSSGSAAAAL DADCSLKQNL RLAGKGTAEP
60 70 80 90 100
HSASDAGMKR ALGRRKSLWF RLRKILLCVL GFYIAIPFLV KLCPGIQAKL
110 120 130 140 150
IFLNFVRVPY FIDLKKPQDQ GLNHTCNYYL QPEDDVTIGV WHTIPSVWWK
160 170 180 190 200
NAQGKDQMWY EDALASNHAI ILYLHGNAGT RGGDHRVELY KVLSSLGYHV
210 220 230 240 250
VTFDYRGWGD SVGTPSERGM TYDALHVFDW IKARSGDNPV YIWGHSLGTG
260 270 280 290 300
VATNLVRRLC ERETPPDALI LESPFTNIRE EAKSHPFSVI YRYFPGFDWF
310 320 330 340 350
FLDPITSSGI KFANDENMKH ISCPLLILHA EDDPVVPFHL GRKLYNIAAP
360 370 380 390
SRSFRDFKVQ FIPFHSDLGY RHKYIYKSPE LPRILREFLG KSEPERQH
Length:398
Mass (Da):45,270
Last modified:November 28, 2003 - v2
Checksum:iB8B23FA97DB4FF45
GO
Isoform 2 (identifier: Q99LR1-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-219: Missing.

Note: No experimental confirmation available.
Show »
Length:179
Mass (Da):20,880
Checksum:iE1606371821F87CC
GO

Sequence cautioni

The sequence AAH02138.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti386 – 3861R → W in AAH02138 (PubMed:15489334).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 219219Missing in isoform 2. 1 PublicationVSP_057493Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL808125, AL954712 Genomic DNA. Translation: CAM17128.1.
AL954712, AL808125 Genomic DNA. Translation: CAM18377.1.
CH466519 Genomic DNA. Translation: EDL28584.1.
BC002138 mRNA. Translation: AAH02138.1. Different initiation.
BC002263 mRNA. Translation: AAH02263.1.
CCDSiCCDS50742.1. [Q99LR1-1]
RefSeqiNP_077785.2. NM_024465.3. [Q99LR1-1]
UniGeneiMm.112632.

Genome annotation databases

EnsembliENSMUST00000056149; ENSMUSP00000053558; ENSMUSG00000032046. [Q99LR1-1]
GeneIDi76192.
KEGGimmu:76192.
UCSCiuc008muo.2. mouse. [Q99LR1-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL808125, AL954712 Genomic DNA. Translation: CAM17128.1.
AL954712, AL808125 Genomic DNA. Translation: CAM18377.1.
CH466519 Genomic DNA. Translation: EDL28584.1.
BC002138 mRNA. Translation: AAH02138.1. Different initiation.
BC002263 mRNA. Translation: AAH02263.1.
CCDSiCCDS50742.1. [Q99LR1-1]
RefSeqiNP_077785.2. NM_024465.3. [Q99LR1-1]
UniGeneiMm.112632.

3D structure databases

ProteinModelPortaliQ99LR1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ99LR1. 2 interactions.
MINTiMINT-4124321.
STRINGi10090.ENSMUSP00000053558.

Chemistry

BindingDBiQ99LR1.
ChEMBLiCHEMBL5972.
SwissLipidsiSLP:000001041.

Protein family/group databases

ESTHERimouse-abd12. AlphaBeta_hydrolase.
MEROPSiS09.054.

PTM databases

PhosphoSiteiQ99LR1.
SwissPalmiQ99LR1.

Proteomic databases

EPDiQ99LR1.
MaxQBiQ99LR1.
PaxDbiQ99LR1.
PRIDEiQ99LR1.

Protocols and materials databases

DNASUi76192.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000056149; ENSMUSP00000053558; ENSMUSG00000032046. [Q99LR1-1]
GeneIDi76192.
KEGGimmu:76192.
UCSCiuc008muo.2. mouse. [Q99LR1-1]

Organism-specific databases

CTDi26090.
MGIiMGI:1923442. Abhd12.

Phylogenomic databases

eggNOGiKOG1552. Eukaryota.
COG1073. LUCA.
GeneTreeiENSGT00390000006625.
HOGENOMiHOG000230822.
HOVERGENiHBG051152.
InParanoidiQ99LR1.
KOiK13704.
OMAiKYPKGLD.
OrthoDBiEOG75TMCD.
PhylomeDBiQ99LR1.
TreeFamiTF315122.

Enzyme and pathway databases

ReactomeiR-MMU-426048. Arachidonate production from DAG.

Miscellaneous databases

NextBioi344743.
PROiQ99LR1.
SOURCEiSearch...

Gene expression databases

BgeeiQ99LR1.
CleanExiMM_ABHD12.
ExpressionAtlasiQ99LR1. baseline and differential.
GenevisibleiQ99LR1. MM.

Family and domain databases

Gene3Di3.40.50.1820. 2 hits.
InterProiIPR029058. AB_hydrolase.
IPR026605. ABHD12.
IPR022742. Hydrolase_4.
[Graphical view]
PANTHERiPTHR12277:SF61. PTHR12277:SF61. 1 hit.
PfamiPF12146. Hydrolase_4. 1 hit.
[Graphical view]
SUPFAMiSSF53474. SSF53474. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  2. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Tissue: Mammary tumor.
  4. "A comprehensive profile of brain enzymes that hydrolyze the endocannabinoid 2-arachidonoylglycerol."
    Blankman J.L., Simon G.M., Cravatt B.F.
    Chem. Biol. 14:1347-1356(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, CATALYTIC ACTIVITY, SUBCELLULAR LOCATION, TOPOLOGY, GLYCOSYLATION.
  5. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain, Brown adipose tissue, Heart, Kidney, Lung, Pancreas, Spleen and Testis.
  6. Cited for: SUBCELLULAR LOCATION.
  7. "ABHD12 controls brain lysophosphatidylserine pathways that are deregulated in a murine model of the neurodegenerative disease PHARC."
    Blankman J.L., Long J.Z., Trauger S.A., Siuzdak G., Cravatt B.F.
    Proc. Natl. Acad. Sci. U.S.A. 110:1500-1505(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE.

Entry informationi

Entry nameiABD12_MOUSE
AccessioniPrimary (citable) accession number: Q99LR1
Secondary accession number(s): A2ANB4, Q99M06
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 28, 2003
Last sequence update: November 28, 2003
Last modified: May 11, 2016
This is version 109 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.