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Protein

Cleavage stimulation factor subunit 3

Gene

Cstf3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

One of the multiple factors required for polyadenylation and 3'-end cleavage of mammalian pre-mRNAs.By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

mRNA processing

Enzyme and pathway databases

ReactomeiR-MMU-109688. Cleavage of Growing Transcript in the Termination Region.
R-MMU-72163. mRNA Splicing - Major Pathway.
R-MMU-72187. mRNA 3'-end processing.
R-MMU-77595. Processing of Intronless Pre-mRNAs.

Names & Taxonomyi

Protein namesi
Recommended name:
Cleavage stimulation factor subunit 3
Alternative name(s):
CF-1 77 kDa subunit
Cleavage stimulation factor 77 kDa subunit
Short name:
CSTF 77 kDa subunit
Short name:
CstF-77
Gene namesi
Name:Cstf3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:1351825. Cstf3.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 717716Cleavage stimulation factor subunit 3PRO_0000205723Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserineBy similarity
Modified residuei691 – 6911PhosphoserineCombined sources

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ99LI7.
MaxQBiQ99LI7.
PaxDbiQ99LI7.
PRIDEiQ99LI7.

PTM databases

iPTMnetiQ99LI7.
PhosphoSiteiQ99LI7.

Expressioni

Gene expression databases

BgeeiQ99LI7.
GenevisibleiQ99LI7. MM.

Interactioni

Subunit structurei

Homodimer. The CSTF complex is composed of CSTF1 (50 kDa subunit), CSTF2 (64 kDa subunit) and CSTF3 (77 kDa subunit). CSTF3 directly interacts with CSTF1 and CSTF2. Interacts with FIP1L1 (By similarity).By similarity

Protein-protein interaction databases

BioGridi230731. 2 interactions.
IntActiQ99LI7. 1 interaction.
MINTiMINT-4124235.
STRINGi10090.ENSMUSP00000028599.

Structurei

Secondary structure

1
717
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi21 – 277Combined sources
Helixi32 – 4312Combined sources
Helixi47 – 5812Combined sources
Helixi65 – 7713Combined sources
Helixi81 – 9111Combined sources
Turni92 – 943Combined sources
Helixi98 – 11114Combined sources
Turni112 – 1143Combined sources
Helixi118 – 13215Combined sources
Helixi140 – 15213Combined sources
Helixi161 – 17515Combined sources
Helixi183 – 19715Combined sources
Helixi199 – 2079Combined sources
Helixi210 – 22920Combined sources
Beta strandi232 – 2343Combined sources
Helixi243 – 26018Combined sources
Helixi269 – 28719Combined sources
Helixi291 – 31020Combined sources
Helixi315 – 33218Combined sources
Turni333 – 3386Combined sources
Helixi340 – 35213Combined sources
Helixi356 – 36712Combined sources
Beta strandi369 – 3724Combined sources
Helixi375 – 38915Combined sources
Helixi391 – 40212Combined sources
Helixi410 – 42112Combined sources
Helixi426 – 44015Combined sources
Helixi444 – 45512Combined sources
Helixi460 – 47213Combined sources
Beta strandi473 – 4764Combined sources
Helixi478 – 4803Combined sources
Helixi482 – 49514Combined sources
Helixi498 – 51114Combined sources
Turni512 – 5165Combined sources
Helixi520 – 5256Combined sources
Turni526 – 5283Combined sources
Helixi538 – 5425Combined sources
Turni543 – 5453Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2ONDX-ray2.80A/B242-549[»]
2OOEX-ray3.00A21-549[»]
ProteinModelPortaliQ99LI7.
SMRiQ99LI7. Positions 21-549.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ99LI7.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati45 – 7733HAT 1Add
BLAST
Repeati79 – 11032HAT 2Add
BLAST
Repeati117 – 15236HAT 3Add
BLAST
Repeati163 – 19634HAT 4Add
BLAST
Repeati221 – 26141HAT 5Add
BLAST
Repeati271 – 30333HAT 6Add
BLAST
Repeati319 – 35234HAT 7Add
BLAST
Repeati354 – 38734HAT 8Add
BLAST
Repeati458 – 49437HAT 9Add
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi559 – 62668Pro-richAdd
BLAST

Sequence similaritiesi

Contains 9 HAT repeats.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG1914. Eukaryota.
COG5107. LUCA.
GeneTreeiENSGT00390000006758.
HOGENOMiHOG000231786.
HOVERGENiHBG053813.
InParanoidiQ99LI7.
KOiK14408.
OMAiDTNQMIP.
OrthoDBiEOG7QVM21.
PhylomeDBiQ99LI7.
TreeFamiTF105867.

Family and domain databases

InterProiIPR003107. HAT.
IPR008847. Suf.
IPR011990. TPR-like_helical_dom.
[Graphical view]
PfamiPF05843. Suf. 1 hit.
[Graphical view]
SMARTiSM00386. HAT. 10 hits.
[Graphical view]
SUPFAMiSSF48452. SSF48452. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q99LI7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSGDAAAEQA AEYVPEKVKK AEKKLEENPY DLDAWSILIR EAQNQPIDKA
60 70 80 90 100
RKTYERLVAQ FPSSGRFWKL YIEAEIKAKN YDKVEKLFQR CLMKVLHIDL
110 120 130 140 150
WKCYLSYVRE TKGKLPSYKE KMAQAYDFAL DKIGMEIMSY QIWVDYINFL
160 170 180 190 200
KGVEAVGSYA ENQRITAVRR VYQRGCVNPM INIEQLWRDY NKYEEGINIH
210 220 230 240 250
LAKKMIEDRS RDYMNARRVA KEYETVMKGL DRNAPSVPPQ NTPQEAQQVD
260 270 280 290 300
MWKKYIQWEK SNPLRTEDQT LITKRVMFAY EQCLLVLGHH PDIWYEAAQY
310 320 330 340 350
LEQSSKLLAE KGDMNNAKLF SDEAANIYER AISTLLKKNM LLYFAYADYE
360 370 380 390 400
ESRMKYEKVH SIYNRLLAIE DIDPTLVYIQ YMKFARRAEG IKSGRMIFKK
410 420 430 440 450
AREDARTRHH VYVTAALMEY YCSKDKSVAF KIFELGLKKY GDIPEYVLAY
460 470 480 490 500
IDYLSHLNED NNTRVLFERV LTSGSLPPEK SGEIWARFLA FESNIGDLAS
510 520 530 540 550
ILKVEKRRFT AFREEYEGKE TALLVDRYKF MDLYPCSASE LKALGYKDVS
560 570 580 590 600
RAKLAAIIPD PVVAPSIVPV LKDEVDRKPE YPKPDTQQMI PFQPRHLAPP
610 620 630 640 650
GLHPVPGGVF PVPPAAVVLM KLLPPPICFQ GPFVQVDELM EIFRRCKIPN
660 670 680 690 700
TVEEAVRIIT GGAPELAVEG NGPVESSAVL TKAVKRPNED SDEDEEKGAV
710
VPPVHDIYRA RQQKRIR
Length:717
Mass (Da):82,877
Last modified:June 1, 2001 - v1
Checksum:i40CF9CC39D496645
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC003241 mRNA. Translation: AAH03241.1.
CCDSiCCDS16490.1.
RefSeqiNP_663504.1. NM_145529.3.
UniGeneiMm.259876.
Mm.479443.

Genome annotation databases

EnsembliENSMUST00000028599; ENSMUSP00000028599; ENSMUSG00000027176.
GeneIDi228410.
KEGGimmu:228410.
UCSCiuc008ljz.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC003241 mRNA. Translation: AAH03241.1.
CCDSiCCDS16490.1.
RefSeqiNP_663504.1. NM_145529.3.
UniGeneiMm.259876.
Mm.479443.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2ONDX-ray2.80A/B242-549[»]
2OOEX-ray3.00A21-549[»]
ProteinModelPortaliQ99LI7.
SMRiQ99LI7. Positions 21-549.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi230731. 2 interactions.
IntActiQ99LI7. 1 interaction.
MINTiMINT-4124235.
STRINGi10090.ENSMUSP00000028599.

PTM databases

iPTMnetiQ99LI7.
PhosphoSiteiQ99LI7.

Proteomic databases

EPDiQ99LI7.
MaxQBiQ99LI7.
PaxDbiQ99LI7.
PRIDEiQ99LI7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000028599; ENSMUSP00000028599; ENSMUSG00000027176.
GeneIDi228410.
KEGGimmu:228410.
UCSCiuc008ljz.2. mouse.

Organism-specific databases

CTDi1479.
MGIiMGI:1351825. Cstf3.

Phylogenomic databases

eggNOGiKOG1914. Eukaryota.
COG5107. LUCA.
GeneTreeiENSGT00390000006758.
HOGENOMiHOG000231786.
HOVERGENiHBG053813.
InParanoidiQ99LI7.
KOiK14408.
OMAiDTNQMIP.
OrthoDBiEOG7QVM21.
PhylomeDBiQ99LI7.
TreeFamiTF105867.

Enzyme and pathway databases

ReactomeiR-MMU-109688. Cleavage of Growing Transcript in the Termination Region.
R-MMU-72163. mRNA Splicing - Major Pathway.
R-MMU-72187. mRNA 3'-end processing.
R-MMU-77595. Processing of Intronless Pre-mRNAs.

Miscellaneous databases

ChiTaRSiCstf3. mouse.
EvolutionaryTraceiQ99LI7.
PROiQ99LI7.
SOURCEiSearch...

Gene expression databases

BgeeiQ99LI7.
GenevisibleiQ99LI7. MM.

Family and domain databases

InterProiIPR003107. HAT.
IPR008847. Suf.
IPR011990. TPR-like_helical_dom.
[Graphical view]
PfamiPF05843. Suf. 1 hit.
[Graphical view]
SMARTiSM00386. HAT. 10 hits.
[Graphical view]
SUPFAMiSSF48452. SSF48452. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Mammary tumor.
  2. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-691, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.
  3. "The phagosomal proteome in interferon-gamma-activated macrophages."
    Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
    Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-691, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  4. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-691, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain, Kidney, Liver, Lung, Pancreas, Spleen and Testis.

Entry informationi

Entry nameiCSTF3_MOUSE
AccessioniPrimary (citable) accession number: Q99LI7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 19, 2005
Last sequence update: June 1, 2001
Last modified: June 8, 2016
This is version 115 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.