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Q99LI7 (CSTF3_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified November 16, 2011. Version 79. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Cleavage stimulation factor subunit 3
Alternative name(s):
CF-1 77 kDa subunit
Cleavage stimulation factor 77 kDa subunit
Short name=CSTF 77 kDa subunit
Short name=CstF-77
Gene names
Name:Cstf3
OrganismMus musculus (Mouse)
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length717 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

One of the multiple factors required for polyadenylation and 3'-end cleavage of mammalian pre-mRNAs By similarity.

Subunit structure

Homodimer. The CSTF complex is composed of CSTF1 (50 kDa subunit), CSTF2 (64 kDa subunit) and CSTF3 (77 kDa subunit). CSTF3 directly interacts with CSTF1 and CSTF2. Interacts with FIP1L1 By similarity.

Subcellular location

Nucleus By similarity.

Sequence similarities

Contains 9 HAT repeats.

Ontologies

Keywords
   Biological processmRNA processing
   Cellular componentNucleus
   DomainRepeat
   PTMPhosphoprotein
   Technical term3D-structure
Complete proteome
Reference proteome
Gene Ontology (GO)
   Biological processmRNA processing

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentnucleus

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionbinding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 717717Cleavage stimulation factor subunit 3
PRO_0000205723

Regions

Repeat45 – 7733HAT 1
Repeat79 – 11032HAT 2
Repeat117 – 15236HAT 3
Repeat163 – 19634HAT 4
Repeat221 – 26141HAT 5
Repeat271 – 30333HAT 6
Repeat319 – 35234HAT 7
Repeat354 – 38734HAT 8
Repeat458 – 49437HAT 9
Compositional bias559 – 62668Pro-rich

Amino acid modifications

Modified residue6911Phosphoserine Ref.2 Ref.3

Secondary structure

1 ...................................................................... 717
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q99LI7 [UniParc].

Last modified June 1, 2001. Version 1.
Checksum: 40CF9CC39D496645

FASTA71782,877
        10         20         30         40         50         60 
MSGDAAAEQA AEYVPEKVKK AEKKLEENPY DLDAWSILIR EAQNQPIDKA RKTYERLVAQ 

        70         80         90        100        110        120 
FPSSGRFWKL YIEAEIKAKN YDKVEKLFQR CLMKVLHIDL WKCYLSYVRE TKGKLPSYKE 

       130        140        150        160        170        180 
KMAQAYDFAL DKIGMEIMSY QIWVDYINFL KGVEAVGSYA ENQRITAVRR VYQRGCVNPM 

       190        200        210        220        230        240 
INIEQLWRDY NKYEEGINIH LAKKMIEDRS RDYMNARRVA KEYETVMKGL DRNAPSVPPQ 

       250        260        270        280        290        300 
NTPQEAQQVD MWKKYIQWEK SNPLRTEDQT LITKRVMFAY EQCLLVLGHH PDIWYEAAQY 

       310        320        330        340        350        360 
LEQSSKLLAE KGDMNNAKLF SDEAANIYER AISTLLKKNM LLYFAYADYE ESRMKYEKVH 

       370        380        390        400        410        420 
SIYNRLLAIE DIDPTLVYIQ YMKFARRAEG IKSGRMIFKK AREDARTRHH VYVTAALMEY 

       430        440        450        460        470        480 
YCSKDKSVAF KIFELGLKKY GDIPEYVLAY IDYLSHLNED NNTRVLFERV LTSGSLPPEK 

       490        500        510        520        530        540 
SGEIWARFLA FESNIGDLAS ILKVEKRRFT AFREEYEGKE TALLVDRYKF MDLYPCSASE 

       550        560        570        580        590        600 
LKALGYKDVS RAKLAAIIPD PVVAPSIVPV LKDEVDRKPE YPKPDTQQMI PFQPRHLAPP 

       610        620        630        640        650        660 
GLHPVPGGVF PVPPAAVVLM KLLPPPICFQ GPFVQVDELM EIFRRCKIPN TVEEAVRIIT 

       670        680        690        700        710 
GGAPELAVEG NGPVESSAVL TKAVKRPNED SDEDEEKGAV VPPVHDIYRA RQQKRIR

« Hide

References

[1]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: FVB/N.
Tissue: Mammary tumor.
[2]"The phagosomal proteome in interferon-gamma-activated macrophages."
Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
Immunity 30:143-154(2009) [PubMed: 19144319] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-691, MASS SPECTROMETRY.
Tissue: Macrophage.
[3]"Solid tumor proteome and phosphoproteome analysis by high resolution mass spectrometry."
Zanivan S., Gnad F., Wickstroem S.A., Geiger T., Macek B., Cox J., Faessler R., Mann M.
J. Proteome Res. 7:5314-5326(2008) [PubMed: 19367708] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-691, MASS SPECTROMETRY.
Tissue: Melanoma.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		BC003241 mRNA. Translation: AAH03241.1.
IPIIPI00116929.
RefSeqNP_663504.1. NM_145529.3.
UniGeneMm.259876.
Mm.479443.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2ONDX-ray2.80A/B242-549[»]
2OOEX-ray3.00A21-549[»]
ProteinModelPortalQ99LI7.
SMRQ99LI7. Positions 21-549.
ModBaseSearch...

Protein-protein interaction databases

STRINGQ99LI7.

PTM databases

PhosphoSiteQ99LI7.

Proteomic databases

PRIDEQ99LI7.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000028599; ENSMUSP00000028599; ENSMUSG00000027176.
GeneID228410.
KEGGmmu:228410.
NMPDRfig|10090.3.peg.6492.

Organism-specific databases

CTD1479.
MGIMGI:1351825. Cstf3.

Phylogenomic databases

GeneTreeENSGT00390000006758.
HOGENOMHBG314518.
HOVERGENHBG053813.
InParanoidQ99LI7.
OMAEFESNIG.
OrthoDBEOG47H5PF.
PhylomeDBQ99LI7.

Gene expression databases

ArrayExpressQ99LI7.
BgeeQ99LI7.
GenevestigatorQ99LI7.
GermOnlineENSMUSG00000027176. Mus musculus.

Family and domain databases

InterProIPR003107. HAT.
IPR008847. Suf.
IPR011990. TPR-like_helical.
[Graphical view]
Gene3DG3DSA:1.25.40.10. TPR-like_helical. 2 hits.
KOK14408.
PfamPF05843. Suf. 1 hit.
[Graphical view]
SMARTSM00386. HAT. 10 hits.
[Graphical view]
ProtoNetSearch...

Other

NextBio378988.
SOURCESearch...

Entry information

Entry nameCSTF3_MOUSE
AccessionPrimary (citable) accession number: Q99LI7
Entry history
Integrated into UniProtKB/Swiss-Prot: July 19, 2005
Last sequence update: June 1, 2001
Last modified: November 16, 2011
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

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