Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Cleavage stimulation factor subunit 3

Gene

Cstf3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

One of the multiple factors required for polyadenylation and 3'-end cleavage of mammalian pre-mRNAs.By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

mRNA processing

Enzyme and pathway databases

ReactomeiR-MMU-109688. Cleavage of Growing Transcript in the Termination Region.
R-MMU-72163. mRNA Splicing - Major Pathway.
R-MMU-72187. mRNA 3'-end processing.
R-MMU-77595. Processing of Intronless Pre-mRNAs.

Names & Taxonomyi

Protein namesi
Recommended name:
Cleavage stimulation factor subunit 3
Alternative name(s):
CF-1 77 kDa subunit
Cleavage stimulation factor 77 kDa subunit
Short name:
CSTF 77 kDa subunit
Short name:
CstF-77
Gene namesi
Name:Cstf3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:1351825. Cstf3.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00002057232 – 717Cleavage stimulation factor subunit 3Add BLAST716

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineBy similarity1
Modified residuei691PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ99LI7.
MaxQBiQ99LI7.
PaxDbiQ99LI7.
PeptideAtlasiQ99LI7.
PRIDEiQ99LI7.

PTM databases

iPTMnetiQ99LI7.
PhosphoSitePlusiQ99LI7.

Expressioni

Gene expression databases

BgeeiENSMUSG00000027176.
GenevisibleiQ99LI7. MM.

Interactioni

Subunit structurei

Homodimer. The CSTF complex is composed of CSTF1 (50 kDa subunit), CSTF2 (64 kDa subunit) and CSTF3 (77 kDa subunit). CSTF3 directly interacts with CSTF1 and CSTF2. Interacts with FIP1L1 (By similarity).By similarity

Protein-protein interaction databases

BioGridi230731. 2 interactors.
IntActiQ99LI7. 1 interactor.
MINTiMINT-4124235.
STRINGi10090.ENSMUSP00000028599.

Structurei

Secondary structure

1717
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi21 – 27Combined sources7
Helixi32 – 43Combined sources12
Helixi47 – 58Combined sources12
Helixi65 – 77Combined sources13
Helixi81 – 91Combined sources11
Turni92 – 94Combined sources3
Helixi98 – 111Combined sources14
Turni112 – 114Combined sources3
Helixi118 – 132Combined sources15
Helixi140 – 152Combined sources13
Helixi161 – 175Combined sources15
Helixi183 – 197Combined sources15
Helixi199 – 207Combined sources9
Helixi210 – 229Combined sources20
Beta strandi232 – 234Combined sources3
Helixi243 – 260Combined sources18
Helixi269 – 287Combined sources19
Helixi291 – 310Combined sources20
Helixi315 – 332Combined sources18
Turni333 – 338Combined sources6
Helixi340 – 352Combined sources13
Helixi356 – 367Combined sources12
Beta strandi369 – 372Combined sources4
Helixi375 – 389Combined sources15
Helixi391 – 402Combined sources12
Helixi410 – 421Combined sources12
Helixi426 – 440Combined sources15
Helixi444 – 455Combined sources12
Helixi460 – 472Combined sources13
Beta strandi473 – 476Combined sources4
Helixi478 – 480Combined sources3
Helixi482 – 495Combined sources14
Helixi498 – 511Combined sources14
Turni512 – 516Combined sources5
Helixi520 – 525Combined sources6
Turni526 – 528Combined sources3
Helixi538 – 542Combined sources5
Turni543 – 545Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2ONDX-ray2.80A/B242-549[»]
2OOEX-ray3.00A21-549[»]
ProteinModelPortaliQ99LI7.
SMRiQ99LI7.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ99LI7.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati45 – 77HAT 1Add BLAST33
Repeati79 – 110HAT 2Add BLAST32
Repeati117 – 152HAT 3Add BLAST36
Repeati163 – 196HAT 4Add BLAST34
Repeati221 – 261HAT 5Add BLAST41
Repeati271 – 303HAT 6Add BLAST33
Repeati319 – 352HAT 7Add BLAST34
Repeati354 – 387HAT 8Add BLAST34
Repeati458 – 494HAT 9Add BLAST37

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi559 – 626Pro-richAdd BLAST68

Sequence similaritiesi

Contains 9 HAT repeats.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG1914. Eukaryota.
COG5107. LUCA.
GeneTreeiENSGT00390000006758.
HOGENOMiHOG000231786.
HOVERGENiHBG053813.
InParanoidiQ99LI7.
KOiK14408.
OMAiDTNQMIP.
OrthoDBiEOG091G02LY.
PhylomeDBiQ99LI7.
TreeFamiTF105867.

Family and domain databases

Gene3Di1.25.40.10. 1 hit.
InterProiIPR003107. HAT.
IPR008847. Suf.
IPR011990. TPR-like_helical_dom.
[Graphical view]
PfamiPF05843. Suf. 1 hit.
[Graphical view]
SMARTiSM00386. HAT. 10 hits.
[Graphical view]
SUPFAMiSSF48452. SSF48452. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q99LI7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSGDAAAEQA AEYVPEKVKK AEKKLEENPY DLDAWSILIR EAQNQPIDKA
60 70 80 90 100
RKTYERLVAQ FPSSGRFWKL YIEAEIKAKN YDKVEKLFQR CLMKVLHIDL
110 120 130 140 150
WKCYLSYVRE TKGKLPSYKE KMAQAYDFAL DKIGMEIMSY QIWVDYINFL
160 170 180 190 200
KGVEAVGSYA ENQRITAVRR VYQRGCVNPM INIEQLWRDY NKYEEGINIH
210 220 230 240 250
LAKKMIEDRS RDYMNARRVA KEYETVMKGL DRNAPSVPPQ NTPQEAQQVD
260 270 280 290 300
MWKKYIQWEK SNPLRTEDQT LITKRVMFAY EQCLLVLGHH PDIWYEAAQY
310 320 330 340 350
LEQSSKLLAE KGDMNNAKLF SDEAANIYER AISTLLKKNM LLYFAYADYE
360 370 380 390 400
ESRMKYEKVH SIYNRLLAIE DIDPTLVYIQ YMKFARRAEG IKSGRMIFKK
410 420 430 440 450
AREDARTRHH VYVTAALMEY YCSKDKSVAF KIFELGLKKY GDIPEYVLAY
460 470 480 490 500
IDYLSHLNED NNTRVLFERV LTSGSLPPEK SGEIWARFLA FESNIGDLAS
510 520 530 540 550
ILKVEKRRFT AFREEYEGKE TALLVDRYKF MDLYPCSASE LKALGYKDVS
560 570 580 590 600
RAKLAAIIPD PVVAPSIVPV LKDEVDRKPE YPKPDTQQMI PFQPRHLAPP
610 620 630 640 650
GLHPVPGGVF PVPPAAVVLM KLLPPPICFQ GPFVQVDELM EIFRRCKIPN
660 670 680 690 700
TVEEAVRIIT GGAPELAVEG NGPVESSAVL TKAVKRPNED SDEDEEKGAV
710
VPPVHDIYRA RQQKRIR
Length:717
Mass (Da):82,877
Last modified:June 1, 2001 - v1
Checksum:i40CF9CC39D496645
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC003241 mRNA. Translation: AAH03241.1.
CCDSiCCDS16490.1.
RefSeqiNP_663504.1. NM_145529.3.
UniGeneiMm.259876.
Mm.479443.

Genome annotation databases

EnsembliENSMUST00000028599; ENSMUSP00000028599; ENSMUSG00000027176.
GeneIDi228410.
KEGGimmu:228410.
UCSCiuc008ljz.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC003241 mRNA. Translation: AAH03241.1.
CCDSiCCDS16490.1.
RefSeqiNP_663504.1. NM_145529.3.
UniGeneiMm.259876.
Mm.479443.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2ONDX-ray2.80A/B242-549[»]
2OOEX-ray3.00A21-549[»]
ProteinModelPortaliQ99LI7.
SMRiQ99LI7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi230731. 2 interactors.
IntActiQ99LI7. 1 interactor.
MINTiMINT-4124235.
STRINGi10090.ENSMUSP00000028599.

PTM databases

iPTMnetiQ99LI7.
PhosphoSitePlusiQ99LI7.

Proteomic databases

EPDiQ99LI7.
MaxQBiQ99LI7.
PaxDbiQ99LI7.
PeptideAtlasiQ99LI7.
PRIDEiQ99LI7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000028599; ENSMUSP00000028599; ENSMUSG00000027176.
GeneIDi228410.
KEGGimmu:228410.
UCSCiuc008ljz.2. mouse.

Organism-specific databases

CTDi1479.
MGIiMGI:1351825. Cstf3.

Phylogenomic databases

eggNOGiKOG1914. Eukaryota.
COG5107. LUCA.
GeneTreeiENSGT00390000006758.
HOGENOMiHOG000231786.
HOVERGENiHBG053813.
InParanoidiQ99LI7.
KOiK14408.
OMAiDTNQMIP.
OrthoDBiEOG091G02LY.
PhylomeDBiQ99LI7.
TreeFamiTF105867.

Enzyme and pathway databases

ReactomeiR-MMU-109688. Cleavage of Growing Transcript in the Termination Region.
R-MMU-72163. mRNA Splicing - Major Pathway.
R-MMU-72187. mRNA 3'-end processing.
R-MMU-77595. Processing of Intronless Pre-mRNAs.

Miscellaneous databases

ChiTaRSiCstf3. mouse.
EvolutionaryTraceiQ99LI7.
PROiQ99LI7.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000027176.
GenevisibleiQ99LI7. MM.

Family and domain databases

Gene3Di1.25.40.10. 1 hit.
InterProiIPR003107. HAT.
IPR008847. Suf.
IPR011990. TPR-like_helical_dom.
[Graphical view]
PfamiPF05843. Suf. 1 hit.
[Graphical view]
SMARTiSM00386. HAT. 10 hits.
[Graphical view]
SUPFAMiSSF48452. SSF48452. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiCSTF3_MOUSE
AccessioniPrimary (citable) accession number: Q99LI7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 19, 2005
Last sequence update: June 1, 2001
Last modified: November 30, 2016
This is version 120 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.