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Q99LI5 (ZN281_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 117. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Zinc finger protein 281
Gene names
Name:Znf281
Synonyms:Zfp281
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length893 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Transcription repressor that plays a role in regulation of embryonic stem cells (ESCs) differentiation. Required for ESCs differentiation and acts by mediating autorepression of NANOG in ESCs: binds to the NANOG promoter and promotes association of NANOG protein to its own promoter and recruits the NuRD complex, which deacetylates histones. Not required for establishement and maintenance of ESCs. Represses the transcription of a number of genes including GAST, ODC1 and VIM. Binds to the G-rich box in the enhancer region of these genes. Ref.5 Ref.7 Ref.8

Subunit structure

Interacts with NANOG. Associates with the NuRD complex. Ref.7 Ref.8

Subcellular location

Nucleus Probable.

Disruption phenotype

Embryos die between embryonic day 7.5 (E7.5) and E8.5, due to dysregulation of pluripotency and lineage specific markers in embryonic stem cells (ESCs). Embryos show abnormal embryonic body differentiation. Ref.7

Sequence similarities

Belongs to the krueppel C2H2-type zinc-finger protein family.

Contains 4 C2H2-type zinc fingers.

Ontologies

Keywords
   Biological processDifferentiation
Transcription
Transcription regulation
   Cellular componentNucleus
   DomainRepeat
Zinc-finger
   LigandDNA-binding
Metal-binding
Zinc
   Molecular functionRepressor
   PTMPhosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processembryonic body morphogenesis

Inferred from mutant phenotype Ref.7. Source: UniProtKB

negative regulation of gene expression

Inferred from sequence or structural similarity. Source: UniProtKB

negative regulation of transcription, DNA-templated

Inferred from sequence or structural similarity. Source: UniProtKB

positive regulation of transcription, DNA-templated

Inferred from sequence or structural similarity. Source: UniProtKB

stem cell differentiation

Inferred from mutant phenotype Ref.7Ref.8. Source: UniProtKB

transcription, DNA-templated

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentnucleus

Inferred from sequence or structural similarity. Source: UniProtKB

   Molecular_functioncore promoter binding

Inferred from direct assay Ref.8. Source: UniProtKB

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

protein binding

Inferred from physical interaction Ref.7Ref.8. Source: UniProtKB

sequence-specific DNA binding

Inferred from sequence or structural similarity. Source: UniProtKB

sequence-specific DNA binding transcription factor activity

Inferred from mutant phenotype Ref.7Ref.8. Source: UniProtKB

transcription corepressor activity

Inferred from mutant phenotype Ref.7Ref.8. Source: UniProtKB

transcription regulatory region DNA binding

Inferred from sequence or structural similarity. Source: UniProtKB

Complete GO annotation...

Binary interactions

With

Entry

#Exp.

IntAct

Notes

NanogQ80Z645EBI-2312719,EBI-2312517

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 893893Zinc finger protein 281
PRO_0000420492

Regions

Zinc finger258 – 28023C2H2-type 1
Zinc finger286 – 30823C2H2-type 2
Zinc finger314 – 33623C2H2-type 3
Zinc finger342 – 36423C2H2-type 4; atypical
Compositional bias4 – 3633Gly-rich
Compositional bias89 – 957Poly-Pro
Compositional bias140 – 1478His-rich

Amino acid modifications

Modified residue3921Phosphoserine By similarity
Modified residue4811Phosphoserine By similarity
Modified residue7821Phosphoserine By similarity
Modified residue8051Phosphoserine By similarity

Experimental info

Sequence conflict1941V → A in BAE33992. Ref.1
Sequence conflict4181G → S in BAE33992. Ref.1
Sequence conflict4341Y → C in CAJ18408. Ref.2
Sequence conflict4391P → S in BAE33992. Ref.1
Sequence conflict7741T → S in CAJ18408. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Q99LI5 [UniParc].

Last modified June 1, 2001. Version 1.
Checksum: 7F4EBA44C2CC2AF2

FASTA89396,685
        10         20         30         40         50         60 
MKIGSGFLSG GGGPSSSGGS GSGGSSGSAS GGSGGGRRAE MEPTFPQSMV MFNHRLPPVT 

        70         80         90        100        110        120 
SFTRPAGTAA PPPQCVLSSS TSAAPAAEPP PPPAPDMTFK KEPAASAAAF PSQRTSWGFL 

       130        140        150        160        170        180 
QSLVSIKQEK PADPEEQPSH HHHHHHHYGG LFAGAEERSP GLGGGEGGSH GVIQDLSLLH 

       190        200        210        220        230        240 
QHAQQQPAQH HRDVLLSSGS RTDEHGNQEP KQDANVKKAK RPKPESQGIK AKRKPSASSK 

       250        260        270        280        290        300 
PLVGEGEGAV LSPSQKPHIC DHCSAAFRSS YHLRRHVLIH TGERPFQCSQ CSMGFIQKYL 

       310        320        330        340        350        360 
LQRHEKIHSR EKPFGCDQCS MKFIQKYHME RHKRTHSGEK PYKCDTCQQY FSRTDRLLKH 

       370        380        390        400        410        420 
RRTCGEAIAK GAASAEPGSS NHNSMGNLAV LSQGNTSSSR RKSKSKSIAI ENKEHKTGKT 

       430        440        450        460        470        480 
NESQMSNNIN MQSYSVEMPT VSTSGSIIGT GIDELQKRVP KLIFKKGSRK NADKSYLNFV 

       490        500        510        520        530        540 
SPLPDVVGQK SLSGKPGGSL GIVSNNSVET ISLLQSTSGK QGPISSNYDD AMQFSKKRRY 

       550        560        570        580        590        600 
LPTASSNSAF SINVGHMVSQ QSVIQSAGVS VLDNEAPLSL IDSSALNAEI KSCHDKSGIP 

       610        620        630        640        650        660 
DEVLQSILDQ YSGKSETQKE DPFNLTEPRV DLHTSGEHSE LVQEENLSPG TQTPSNDKTS 

       670        680        690        700        710        720 
MLQEYSKYLQ QAFEKSTNAG FTLGHGFQFV SLSSPLHNHT LFPEKQIYTT SPLECGFGQS 

       730        740        750        760        770        780 
VTSVLPSSLP KPPFGMLFGS QPGLYLSALD ATHQQLTPSQ ELDDLIDSQK NLETSSAFQS 

       790        800        810        820        830        840 
SSQKLTSQKE QQKNLESSTS FQIPSQELAS QIDPQKDIEP RTTYQIENFA QAFGSQFKSG 

       850        860        870        880        890 
SRVPMTFITN SNGEVDHRVR TSVSDFSGYT NMMSDVSEPC STRVKTPTSQ SYR 

« Hide

References

« Hide 'large scale' references
[1]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6J and NOD.
Tissue: Heart and Spleen.
[2]"Cloning of mouse full open reading frames in Gateway(R) system entry vector (pDONR201)."
Ebert L., Muenstermann E., Schatten R., Henze S., Bohn E., Mollenhauer J., Wiemann S., Schick M., Korn B.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[3]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: FVB/N.
Tissue: Embryo and Mammary tumor.
[5]"The transcription factor Zfp281 controls embryonic stem cell pluripotency by direct activation and repression of target genes."
Wang Z.X., Teh C.H., Chan C.M., Chu C., Rossbach M., Kunarso G., Allapitchay T.B., Wong K.Y., Stanton L.W.
Stem Cells 26:2791-2799(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, DNA-BINDING.
[6]"The phagosomal proteome in interferon-gamma-activated macrophages."
Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[7]"Zfp281 functions as a transcriptional repressor for pluripotency of mouse embryonic stem cells."
Fidalgo M., Shekar P.C., Ang Y.S., Fujiwara Y., Orkin S.H., Wang J.
Stem Cells 29:1705-1716(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, DNA-BINDING, DISRUPTION PHENOTYPE, INTERACTION WITH NANOG.
[8]"Zfp281 mediates Nanog autorepression through recruitment of the NuRD complex and inhibits somatic cell reprogramming."
Fidalgo M., Faiola F., Pereira C.F., Ding J., Saunders A., Gingold J., Schaniel C., Lemischka I.R., Silva J.C., Wang J.
Proc. Natl. Acad. Sci. U.S.A. 109:16202-16207(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH NANOG, INTERACTION WITH THE NURD COMPLEX.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AK142218 mRNA. Translation: BAE24981.1.
AK157189 mRNA. Translation: BAE33992.1.
CT010200 mRNA. Translation: CAJ18408.1.
AC126606 Genomic DNA. No translation available.
BC003243 mRNA. Translation: AAH03243.1.
BC062153 mRNA. Translation: AAH62153.1.
CCDSCCDS15327.1.
RefSeqNP_001153723.1. NM_001160251.1.
NP_808311.1. NM_177643.4.
UniGeneMm.62521.

3D structure databases

ProteinModelPortalQ99LI5.
SMRQ99LI5. Positions 249-363.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

DIPDIP-29927N.
IntActQ99LI5. 3 interactions.
STRING10090.ENSMUSP00000039003.

PTM databases

PhosphoSiteQ99LI5.

Proteomic databases

MaxQBQ99LI5.
PRIDEQ99LI5.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000047734; ENSMUSP00000039003; ENSMUSG00000041483.
ENSMUST00000112046; ENSMUSP00000107677; ENSMUSG00000041483.
GeneID226442.
KEGGmmu:226442.
UCSCuc007cuw.2. mouse.

Organism-specific databases

CTD226442.
MGIMGI:3029290. Zfp281.

Phylogenomic databases

GeneTreeENSGT00530000063238.
HOVERGENHBG055321.
InParanoidQ99LI5.
OMAQKDIEPR.
OrthoDBEOG7VDXNJ.
PhylomeDBQ99LI5.
TreeFamTF331779.

Gene expression databases

ArrayExpressQ99LI5.
BgeeQ99LI5.
GenevestigatorQ99LI5.

Family and domain databases

Gene3D3.30.160.60. 4 hits.
InterProIPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
SMARTSM00355. ZnF_C2H2. 4 hits.
[Graphical view]
PROSITEPS00028. ZINC_FINGER_C2H2_1. 3 hits.
PS50157. ZINC_FINGER_C2H2_2. 4 hits.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSZfp281. mouse.
NextBio378172.
PROQ99LI5.
SOURCESearch...

Entry information

Entry nameZN281_MOUSE
AccessionPrimary (citable) accession number: Q99LI5
Secondary accession number(s): Q3U063, Q4FK52
Entry history
Integrated into UniProtKB/Swiss-Prot: November 28, 2012
Last sequence update: June 1, 2001
Last modified: July 9, 2014
This is version 117 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot