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Q99LI5

- ZN281_MOUSE

UniProt

Q99LI5 - ZN281_MOUSE

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Protein
Zinc finger protein 281
Gene
Znf281, Zfp281
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Transcription repressor that plays a role in regulation of embryonic stem cells (ESCs) differentiation. Required for ESCs differentiation and acts by mediating autorepression of NANOG in ESCs: binds to the NANOG promoter and promotes association of NANOG protein to its own promoter and recruits the NuRD complex, which deacetylates histones. Not required for establishement and maintenance of ESCs. Represses the transcription of a number of genes including GAST, ODC1 and VIM. Binds to the G-rich box in the enhancer region of these genes.3 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri258 – 28023C2H2-type 1
Add
BLAST
Zinc fingeri286 – 30823C2H2-type 2
Add
BLAST
Zinc fingeri314 – 33623C2H2-type 3
Add
BLAST
Zinc fingeri342 – 36423C2H2-type 4; atypical
Add
BLAST

GO - Molecular functioni

  1. core promoter binding Source: UniProtKB
  2. metal ion binding Source: UniProtKB-KW
  3. protein binding Source: UniProtKB
  4. sequence-specific DNA binding Source: UniProtKB
  5. sequence-specific DNA binding transcription factor activity Source: UniProtKB
  6. transcription corepressor activity Source: UniProtKB
  7. transcription regulatory region DNA binding Source: UniProtKB

GO - Biological processi

  1. embryonic body morphogenesis Source: UniProtKB
  2. negative regulation of gene expression Source: UniProtKB
  3. negative regulation of transcription, DNA-templated Source: UniProtKB
  4. positive regulation of transcription, DNA-templated Source: UniProtKB
  5. stem cell differentiation Source: UniProtKB
  6. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Repressor

Keywords - Biological processi

Differentiation, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Zinc finger protein 281
Gene namesi
Name:Znf281
Synonyms:Zfp281
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 1

Organism-specific databases

MGIiMGI:3029290. Zfp281.

Subcellular locationi

Nucleus Inferred

GO - Cellular componenti

  1. nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

Embryos die between embryonic day 7.5 (E7.5) and E8.5, due to dysregulation of pluripotency and lineage specific markers in embryonic stem cells (ESCs). Embryos show abnormal embryonic body differentiation.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 893893Zinc finger protein 281
PRO_0000420492Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei392 – 3921Phosphoserine By similarity
Modified residuei481 – 4811Phosphoserine By similarity
Modified residuei782 – 7821Phosphoserine By similarity
Modified residuei805 – 8051Phosphoserine By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ99LI5.
PRIDEiQ99LI5.

PTM databases

PhosphoSiteiQ99LI5.

Expressioni

Gene expression databases

ArrayExpressiQ99LI5.
BgeeiQ99LI5.
GenevestigatoriQ99LI5.

Interactioni

Subunit structurei

Interacts with NANOG. Associates with the NuRD complex.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
NanogQ80Z645EBI-2312719,EBI-2312517

Protein-protein interaction databases

DIPiDIP-29927N.
IntActiQ99LI5. 3 interactions.
STRINGi10090.ENSMUSP00000039003.

Structurei

3D structure databases

ProteinModelPortaliQ99LI5.
SMRiQ99LI5. Positions 249-363.

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi4 – 3633Gly-rich
Add
BLAST
Compositional biasi89 – 957Poly-Pro
Compositional biasi140 – 1478His-rich

Sequence similaritiesi

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

GeneTreeiENSGT00530000063238.
HOVERGENiHBG055321.
InParanoidiQ99LI5.
OMAiQKDIEPR.
OrthoDBiEOG7VDXNJ.
PhylomeDBiQ99LI5.
TreeFamiTF331779.

Family and domain databases

Gene3Di3.30.160.60. 4 hits.
InterProiIPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
SMARTiSM00355. ZnF_C2H2. 4 hits.
[Graphical view]
PROSITEiPS00028. ZINC_FINGER_C2H2_1. 3 hits.
PS50157. ZINC_FINGER_C2H2_2. 4 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q99LI5-1 [UniParc]FASTAAdd to Basket

« Hide

MKIGSGFLSG GGGPSSSGGS GSGGSSGSAS GGSGGGRRAE MEPTFPQSMV    50
MFNHRLPPVT SFTRPAGTAA PPPQCVLSSS TSAAPAAEPP PPPAPDMTFK 100
KEPAASAAAF PSQRTSWGFL QSLVSIKQEK PADPEEQPSH HHHHHHHYGG 150
LFAGAEERSP GLGGGEGGSH GVIQDLSLLH QHAQQQPAQH HRDVLLSSGS 200
RTDEHGNQEP KQDANVKKAK RPKPESQGIK AKRKPSASSK PLVGEGEGAV 250
LSPSQKPHIC DHCSAAFRSS YHLRRHVLIH TGERPFQCSQ CSMGFIQKYL 300
LQRHEKIHSR EKPFGCDQCS MKFIQKYHME RHKRTHSGEK PYKCDTCQQY 350
FSRTDRLLKH RRTCGEAIAK GAASAEPGSS NHNSMGNLAV LSQGNTSSSR 400
RKSKSKSIAI ENKEHKTGKT NESQMSNNIN MQSYSVEMPT VSTSGSIIGT 450
GIDELQKRVP KLIFKKGSRK NADKSYLNFV SPLPDVVGQK SLSGKPGGSL 500
GIVSNNSVET ISLLQSTSGK QGPISSNYDD AMQFSKKRRY LPTASSNSAF 550
SINVGHMVSQ QSVIQSAGVS VLDNEAPLSL IDSSALNAEI KSCHDKSGIP 600
DEVLQSILDQ YSGKSETQKE DPFNLTEPRV DLHTSGEHSE LVQEENLSPG 650
TQTPSNDKTS MLQEYSKYLQ QAFEKSTNAG FTLGHGFQFV SLSSPLHNHT 700
LFPEKQIYTT SPLECGFGQS VTSVLPSSLP KPPFGMLFGS QPGLYLSALD 750
ATHQQLTPSQ ELDDLIDSQK NLETSSAFQS SSQKLTSQKE QQKNLESSTS 800
FQIPSQELAS QIDPQKDIEP RTTYQIENFA QAFGSQFKSG SRVPMTFITN 850
SNGEVDHRVR TSVSDFSGYT NMMSDVSEPC STRVKTPTSQ SYR 893
Length:893
Mass (Da):96,685
Last modified:June 1, 2001 - v1
Checksum:i7F4EBA44C2CC2AF2
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti194 – 1941V → A in BAE33992. 1 Publication
Sequence conflicti418 – 4181G → S in BAE33992. 1 Publication
Sequence conflicti434 – 4341Y → C in CAJ18408. 1 Publication
Sequence conflicti439 – 4391P → S in BAE33992. 1 Publication
Sequence conflicti774 – 7741T → S in CAJ18408. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AK142218 mRNA. Translation: BAE24981.1.
AK157189 mRNA. Translation: BAE33992.1.
CT010200 mRNA. Translation: CAJ18408.1.
AC126606 Genomic DNA. No translation available.
BC003243 mRNA. Translation: AAH03243.1.
BC062153 mRNA. Translation: AAH62153.1.
CCDSiCCDS15327.1.
RefSeqiNP_001153723.1. NM_001160251.1.
NP_808311.1. NM_177643.4.
UniGeneiMm.62521.

Genome annotation databases

EnsembliENSMUST00000047734; ENSMUSP00000039003; ENSMUSG00000041483.
ENSMUST00000112046; ENSMUSP00000107677; ENSMUSG00000041483.
GeneIDi226442.
KEGGimmu:226442.
UCSCiuc007cuw.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AK142218 mRNA. Translation: BAE24981.1 .
AK157189 mRNA. Translation: BAE33992.1 .
CT010200 mRNA. Translation: CAJ18408.1 .
AC126606 Genomic DNA. No translation available.
BC003243 mRNA. Translation: AAH03243.1 .
BC062153 mRNA. Translation: AAH62153.1 .
CCDSi CCDS15327.1.
RefSeqi NP_001153723.1. NM_001160251.1.
NP_808311.1. NM_177643.4.
UniGenei Mm.62521.

3D structure databases

ProteinModelPortali Q99LI5.
SMRi Q99LI5. Positions 249-363.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

DIPi DIP-29927N.
IntActi Q99LI5. 3 interactions.
STRINGi 10090.ENSMUSP00000039003.

PTM databases

PhosphoSitei Q99LI5.

Proteomic databases

MaxQBi Q99LI5.
PRIDEi Q99LI5.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000047734 ; ENSMUSP00000039003 ; ENSMUSG00000041483 .
ENSMUST00000112046 ; ENSMUSP00000107677 ; ENSMUSG00000041483 .
GeneIDi 226442.
KEGGi mmu:226442.
UCSCi uc007cuw.2. mouse.

Organism-specific databases

CTDi 226442.
MGIi MGI:3029290. Zfp281.

Phylogenomic databases

GeneTreei ENSGT00530000063238.
HOVERGENi HBG055321.
InParanoidi Q99LI5.
OMAi QKDIEPR.
OrthoDBi EOG7VDXNJ.
PhylomeDBi Q99LI5.
TreeFami TF331779.

Miscellaneous databases

ChiTaRSi Zfp281. mouse.
NextBioi 378172.
PROi Q99LI5.
SOURCEi Search...

Gene expression databases

ArrayExpressi Q99LI5.
Bgeei Q99LI5.
Genevestigatori Q99LI5.

Family and domain databases

Gene3Di 3.30.160.60. 4 hits.
InterProi IPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view ]
SMARTi SM00355. ZnF_C2H2. 4 hits.
[Graphical view ]
PROSITEi PS00028. ZINC_FINGER_C2H2_1. 3 hits.
PS50157. ZINC_FINGER_C2H2_2. 4 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J and NOD.
    Tissue: Heart and Spleen.
  2. "Cloning of mouse full open reading frames in Gateway(R) system entry vector (pDONR201)."
    Ebert L., Muenstermann E., Schatten R., Henze S., Bohn E., Mollenhauer J., Wiemann S., Schick M., Korn B.
    Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Embryo and Mammary tumor.
  5. "The transcription factor Zfp281 controls embryonic stem cell pluripotency by direct activation and repression of target genes."
    Wang Z.X., Teh C.H., Chan C.M., Chu C., Rossbach M., Kunarso G., Allapitchay T.B., Wong K.Y., Stanton L.W.
    Stem Cells 26:2791-2799(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DNA-BINDING.
  6. "The phagosomal proteome in interferon-gamma-activated macrophages."
    Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
    Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  7. "Zfp281 functions as a transcriptional repressor for pluripotency of mouse embryonic stem cells."
    Fidalgo M., Shekar P.C., Ang Y.S., Fujiwara Y., Orkin S.H., Wang J.
    Stem Cells 29:1705-1716(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DNA-BINDING, DISRUPTION PHENOTYPE, INTERACTION WITH NANOG.
  8. "Zfp281 mediates Nanog autorepression through recruitment of the NuRD complex and inhibits somatic cell reprogramming."
    Fidalgo M., Faiola F., Pereira C.F., Ding J., Saunders A., Gingold J., Schaniel C., Lemischka I.R., Silva J.C., Wang J.
    Proc. Natl. Acad. Sci. U.S.A. 109:16202-16207(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH NANOG, INTERACTION WITH THE NURD COMPLEX.

Entry informationi

Entry nameiZN281_MOUSE
AccessioniPrimary (citable) accession number: Q99LI5
Secondary accession number(s): Q3U063, Q4FK52
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 28, 2012
Last sequence update: June 1, 2001
Last modified: July 9, 2014
This is version 117 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi