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Q99LI5

- ZN281_MOUSE

UniProt

Q99LI5 - ZN281_MOUSE

Protein

Zinc finger protein 281

Gene

Znf281

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 118 (01 Oct 2014)
      Sequence version 1 (01 Jun 2001)
      Previous versions | rss
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    Functioni

    Transcription repressor that plays a role in regulation of embryonic stem cells (ESCs) differentiation. Required for ESCs differentiation and acts by mediating autorepression of NANOG in ESCs: binds to the NANOG promoter and promotes association of NANOG protein to its own promoter and recruits the NuRD complex, which deacetylates histones. Not required for establishement and maintenance of ESCs. Represses the transcription of a number of genes including GAST, ODC1 and VIM. Binds to the G-rich box in the enhancer region of these genes.3 Publications

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri258 – 28023C2H2-type 1PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri286 – 30823C2H2-type 2PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri314 – 33623C2H2-type 3PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri342 – 36423C2H2-type 4; atypicalPROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. core promoter binding Source: UniProtKB
    2. metal ion binding Source: UniProtKB-KW
    3. protein binding Source: UniProtKB
    4. sequence-specific DNA binding Source: UniProtKB
    5. sequence-specific DNA binding transcription factor activity Source: UniProtKB
    6. transcription corepressor activity Source: UniProtKB
    7. transcription regulatory region DNA binding Source: UniProtKB

    GO - Biological processi

    1. embryonic body morphogenesis Source: UniProtKB
    2. negative regulation of gene expression Source: UniProtKB
    3. negative regulation of transcription, DNA-templated Source: UniProtKB
    4. positive regulation of transcription, DNA-templated Source: UniProtKB
    5. stem cell differentiation Source: UniProtKB
    6. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Molecular functioni

    Repressor

    Keywords - Biological processi

    Differentiation, Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding, Metal-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Zinc finger protein 281
    Gene namesi
    Name:Znf281
    Synonyms:Zfp281
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 1

    Organism-specific databases

    MGIiMGI:3029290. Zfp281.

    Subcellular locationi

    Nucleus Curated

    GO - Cellular componenti

    1. nucleus Source: UniProtKB

    Keywords - Cellular componenti

    Nucleus

    Pathology & Biotechi

    Disruption phenotypei

    Embryos die between embryonic day 7.5 (E7.5) and E8.5, due to dysregulation of pluripotency and lineage specific markers in embryonic stem cells (ESCs). Embryos show abnormal embryonic body differentiation.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 893893Zinc finger protein 281PRO_0000420492Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei392 – 3921PhosphoserineBy similarity
    Modified residuei481 – 4811PhosphoserineBy similarity
    Modified residuei782 – 7821PhosphoserineBy similarity
    Modified residuei805 – 8051PhosphoserineBy similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiQ99LI5.
    PRIDEiQ99LI5.

    PTM databases

    PhosphoSiteiQ99LI5.

    Expressioni

    Gene expression databases

    ArrayExpressiQ99LI5.
    BgeeiQ99LI5.
    GenevestigatoriQ99LI5.

    Interactioni

    Subunit structurei

    Interacts with NANOG. Associates with the NuRD complex.2 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    NanogQ80Z645EBI-2312719,EBI-2312517

    Protein-protein interaction databases

    DIPiDIP-29927N.
    IntActiQ99LI5. 3 interactions.
    STRINGi10090.ENSMUSP00000039003.

    Structurei

    3D structure databases

    ProteinModelPortaliQ99LI5.
    SMRiQ99LI5. Positions 249-363.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi4 – 3633Gly-richAdd
    BLAST
    Compositional biasi89 – 957Poly-Pro
    Compositional biasi140 – 1478His-rich

    Sequence similaritiesi

    Contains 4 C2H2-type zinc fingers.PROSITE-ProRule annotation

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri258 – 28023C2H2-type 1PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri286 – 30823C2H2-type 2PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri314 – 33623C2H2-type 3PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri342 – 36423C2H2-type 4; atypicalPROSITE-ProRule annotationAdd
    BLAST

    Keywords - Domaini

    Repeat, Zinc-finger

    Phylogenomic databases

    GeneTreeiENSGT00530000063238.
    HOVERGENiHBG055321.
    InParanoidiQ99LI5.
    OMAiQKDIEPR.
    OrthoDBiEOG7VDXNJ.
    PhylomeDBiQ99LI5.
    TreeFamiTF331779.

    Family and domain databases

    Gene3Di3.30.160.60. 4 hits.
    InterProiIPR007087. Znf_C2H2.
    IPR015880. Znf_C2H2-like.
    IPR013087. Znf_C2H2/integrase_DNA-bd.
    [Graphical view]
    SMARTiSM00355. ZnF_C2H2. 4 hits.
    [Graphical view]
    PROSITEiPS00028. ZINC_FINGER_C2H2_1. 3 hits.
    PS50157. ZINC_FINGER_C2H2_2. 4 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q99LI5-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKIGSGFLSG GGGPSSSGGS GSGGSSGSAS GGSGGGRRAE MEPTFPQSMV    50
    MFNHRLPPVT SFTRPAGTAA PPPQCVLSSS TSAAPAAEPP PPPAPDMTFK 100
    KEPAASAAAF PSQRTSWGFL QSLVSIKQEK PADPEEQPSH HHHHHHHYGG 150
    LFAGAEERSP GLGGGEGGSH GVIQDLSLLH QHAQQQPAQH HRDVLLSSGS 200
    RTDEHGNQEP KQDANVKKAK RPKPESQGIK AKRKPSASSK PLVGEGEGAV 250
    LSPSQKPHIC DHCSAAFRSS YHLRRHVLIH TGERPFQCSQ CSMGFIQKYL 300
    LQRHEKIHSR EKPFGCDQCS MKFIQKYHME RHKRTHSGEK PYKCDTCQQY 350
    FSRTDRLLKH RRTCGEAIAK GAASAEPGSS NHNSMGNLAV LSQGNTSSSR 400
    RKSKSKSIAI ENKEHKTGKT NESQMSNNIN MQSYSVEMPT VSTSGSIIGT 450
    GIDELQKRVP KLIFKKGSRK NADKSYLNFV SPLPDVVGQK SLSGKPGGSL 500
    GIVSNNSVET ISLLQSTSGK QGPISSNYDD AMQFSKKRRY LPTASSNSAF 550
    SINVGHMVSQ QSVIQSAGVS VLDNEAPLSL IDSSALNAEI KSCHDKSGIP 600
    DEVLQSILDQ YSGKSETQKE DPFNLTEPRV DLHTSGEHSE LVQEENLSPG 650
    TQTPSNDKTS MLQEYSKYLQ QAFEKSTNAG FTLGHGFQFV SLSSPLHNHT 700
    LFPEKQIYTT SPLECGFGQS VTSVLPSSLP KPPFGMLFGS QPGLYLSALD 750
    ATHQQLTPSQ ELDDLIDSQK NLETSSAFQS SSQKLTSQKE QQKNLESSTS 800
    FQIPSQELAS QIDPQKDIEP RTTYQIENFA QAFGSQFKSG SRVPMTFITN 850
    SNGEVDHRVR TSVSDFSGYT NMMSDVSEPC STRVKTPTSQ SYR 893
    Length:893
    Mass (Da):96,685
    Last modified:June 1, 2001 - v1
    Checksum:i7F4EBA44C2CC2AF2
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti194 – 1941V → A in BAE33992. (PubMed:16141072)Curated
    Sequence conflicti418 – 4181G → S in BAE33992. (PubMed:16141072)Curated
    Sequence conflicti434 – 4341Y → C in CAJ18408. 1 PublicationCurated
    Sequence conflicti439 – 4391P → S in BAE33992. (PubMed:16141072)Curated
    Sequence conflicti774 – 7741T → S in CAJ18408. 1 PublicationCurated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK142218 mRNA. Translation: BAE24981.1.
    AK157189 mRNA. Translation: BAE33992.1.
    CT010200 mRNA. Translation: CAJ18408.1.
    AC126606 Genomic DNA. No translation available.
    BC003243 mRNA. Translation: AAH03243.1.
    BC062153 mRNA. Translation: AAH62153.1.
    CCDSiCCDS15327.1.
    RefSeqiNP_001153723.1. NM_001160251.1.
    NP_808311.1. NM_177643.4.
    UniGeneiMm.62521.

    Genome annotation databases

    EnsembliENSMUST00000047734; ENSMUSP00000039003; ENSMUSG00000041483.
    ENSMUST00000112046; ENSMUSP00000107677; ENSMUSG00000041483.
    GeneIDi226442.
    KEGGimmu:226442.
    UCSCiuc007cuw.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK142218 mRNA. Translation: BAE24981.1 .
    AK157189 mRNA. Translation: BAE33992.1 .
    CT010200 mRNA. Translation: CAJ18408.1 .
    AC126606 Genomic DNA. No translation available.
    BC003243 mRNA. Translation: AAH03243.1 .
    BC062153 mRNA. Translation: AAH62153.1 .
    CCDSi CCDS15327.1.
    RefSeqi NP_001153723.1. NM_001160251.1.
    NP_808311.1. NM_177643.4.
    UniGenei Mm.62521.

    3D structure databases

    ProteinModelPortali Q99LI5.
    SMRi Q99LI5. Positions 249-363.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-29927N.
    IntActi Q99LI5. 3 interactions.
    STRINGi 10090.ENSMUSP00000039003.

    PTM databases

    PhosphoSitei Q99LI5.

    Proteomic databases

    MaxQBi Q99LI5.
    PRIDEi Q99LI5.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000047734 ; ENSMUSP00000039003 ; ENSMUSG00000041483 .
    ENSMUST00000112046 ; ENSMUSP00000107677 ; ENSMUSG00000041483 .
    GeneIDi 226442.
    KEGGi mmu:226442.
    UCSCi uc007cuw.2. mouse.

    Organism-specific databases

    CTDi 226442.
    MGIi MGI:3029290. Zfp281.

    Phylogenomic databases

    GeneTreei ENSGT00530000063238.
    HOVERGENi HBG055321.
    InParanoidi Q99LI5.
    OMAi QKDIEPR.
    OrthoDBi EOG7VDXNJ.
    PhylomeDBi Q99LI5.
    TreeFami TF331779.

    Miscellaneous databases

    ChiTaRSi Zfp281. mouse.
    NextBioi 378172.
    PROi Q99LI5.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q99LI5.
    Bgeei Q99LI5.
    Genevestigatori Q99LI5.

    Family and domain databases

    Gene3Di 3.30.160.60. 4 hits.
    InterProi IPR007087. Znf_C2H2.
    IPR015880. Znf_C2H2-like.
    IPR013087. Znf_C2H2/integrase_DNA-bd.
    [Graphical view ]
    SMARTi SM00355. ZnF_C2H2. 4 hits.
    [Graphical view ]
    PROSITEi PS00028. ZINC_FINGER_C2H2_1. 3 hits.
    PS50157. ZINC_FINGER_C2H2_2. 4 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J and NOD.
      Tissue: Heart and Spleen.
    2. "Cloning of mouse full open reading frames in Gateway(R) system entry vector (pDONR201)."
      Ebert L., Muenstermann E., Schatten R., Henze S., Bohn E., Mollenhauer J., Wiemann S., Schick M., Korn B.
      Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: FVB/N.
      Tissue: Embryo and Mammary tumor.
    5. "The transcription factor Zfp281 controls embryonic stem cell pluripotency by direct activation and repression of target genes."
      Wang Z.X., Teh C.H., Chan C.M., Chu C., Rossbach M., Kunarso G., Allapitchay T.B., Wong K.Y., Stanton L.W.
      Stem Cells 26:2791-2799(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, DNA-BINDING.
    6. "The phagosomal proteome in interferon-gamma-activated macrophages."
      Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
      Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    7. "Zfp281 functions as a transcriptional repressor for pluripotency of mouse embryonic stem cells."
      Fidalgo M., Shekar P.C., Ang Y.S., Fujiwara Y., Orkin S.H., Wang J.
      Stem Cells 29:1705-1716(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, DNA-BINDING, DISRUPTION PHENOTYPE, INTERACTION WITH NANOG.
    8. "Zfp281 mediates Nanog autorepression through recruitment of the NuRD complex and inhibits somatic cell reprogramming."
      Fidalgo M., Faiola F., Pereira C.F., Ding J., Saunders A., Gingold J., Schaniel C., Lemischka I.R., Silva J.C., Wang J.
      Proc. Natl. Acad. Sci. U.S.A. 109:16202-16207(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH NANOG, INTERACTION WITH THE NURD COMPLEX.

    Entry informationi

    Entry nameiZN281_MOUSE
    AccessioniPrimary (citable) accession number: Q99LI5
    Secondary accession number(s): Q3U063, Q4FK52
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 28, 2012
    Last sequence update: June 1, 2001
    Last modified: October 1, 2014
    This is version 118 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3