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Q99LG0

- UBP16_MOUSE

UniProt

Q99LG0 - UBP16_MOUSE

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Protein
Ubiquitin carboxyl-terminal hydrolase 16
Gene
Usp16
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Specifically deubiquitinates 'Lys-120' of histone H2A (H2AK119Ub), a specific tag for epigenetic transcriptional repression, thereby acting as a coactivator. Deubiquitination of histone H2A is a prerequisite for subsequent phosphorylation at 'Ser-11' of histone H3 (H3S10ph), and is required for chromosome segregation when cells enter into mitosis. In resting B- and T-lymphocytes, phosphorylation by AURKB leads to enhance its activity, thereby maintaining transcription in resting lymphocytes (1 Publication). Regulates Hox gene expression via histone H2A deubiquitination. Prefers nucleosomal substrates. Does not deubiquitinate histone H2B.1 Publication

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi24 – 241Zinc 1 By similarity
Metal bindingi26 – 261Zinc 1 By similarity
Metal bindingi48 – 481Zinc 2 By similarity
Metal bindingi51 – 511Zinc 2 By similarity
Metal bindingi73 – 731Zinc 3 By similarity
Metal bindingi76 – 761Zinc 3 By similarity
Metal bindingi81 – 811Zinc 2 By similarity
Metal bindingi89 – 891Zinc 2 By similarity
Metal bindingi93 – 931Zinc 3 By similarity
Metal bindingi102 – 1021Zinc 3 By similarity
Metal bindingi115 – 1151Zinc 1 By similarity
Metal bindingi118 – 1181Zinc 1 By similarity
Active sitei204 – 2041Nucleophile By similarity
Active sitei759 – 7591Proton acceptor By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri46 – 12479UBP-typeUniRule annotation
Add
BLAST

GO - Molecular functioni

  1. cysteine-type endopeptidase activity Source: UniProtKB
  2. histone binding Source: UniProtKB
  3. protein binding Source: UniProtKB
  4. transcription coactivator activity Source: UniProtKB
  5. ubiquitin binding Source: UniProtKB
  6. ubiquitin thiolesterase activity Source: UniProtKB
  7. ubiquitin-specific protease activity Source: UniProtKB
  8. zinc ion binding Source: UniProtKB

GO - Biological processi

  1. cellular response to DNA damage stimulus Source: UniProtKB
  2. histone H2A K63-linked deubiquitination Source: UniProtKB
  3. histone deubiquitination Source: UniProtKB
  4. mitotic nuclear division Source: UniProtKB
  5. monoubiquitinated histone H2A deubiquitination Source: UniProtKB
  6. positive regulation of transcription from RNA polymerase II promoter Source: UniProtKB
  7. positive regulation of transcription, DNA-templated Source: UniProtKB
  8. positive regulation of translational elongation Source: UniProtKB
  9. protein homotetramerization Source: UniProtKB
  10. regulation of transcription from RNA polymerase II promoter Source: UniProtKB
  11. transcription, DNA-templated Source: UniProtKB-KW
  12. ubiquitin-dependent protein catabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Activator, Chromatin regulator, Hydrolase, Protease, Thiol protease

Keywords - Biological processi

Cell cycle, Cell division, Mitosis, Transcription, Transcription regulation, Ubl conjugation pathway

Keywords - Ligandi

Metal-binding, Zinc

Protein family/group databases

MEROPSiC19.050.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin carboxyl-terminal hydrolase 16 (EC:3.4.19.12)
Alternative name(s):
Deubiquitinating enzyme 16
Ubiquitin thioesterase 16
Ubiquitin-specific-processing protease 16
Gene namesi
Name:Usp16
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 16

Organism-specific databases

MGIiMGI:1921362. Usp16.

Subcellular locationi

Nucleus By similarity UniRule annotation

GO - Cellular componenti

  1. nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 825825Ubiquitin carboxyl-terminal hydrolase 16UniRule annotation
PRO_0000367503Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei414 – 4141Phosphoserine By similarity
Modified residuei520 – 5201Phosphoserine1 Publication

Post-translational modificationi

Phosphorylated at the onset of mitosis and dephosphorylated during the metaphase/anaphase transition. Phosphorylation by AURKB enhances the deubiquitinase activity.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ99LG0.
PRIDEiQ99LG0.

PTM databases

PhosphoSiteiQ99LG0.

Expressioni

Gene expression databases

GenevestigatoriQ99LG0.

Interactioni

Subunit structurei

Homotetramer By similarity.UniRule annotation

Protein-protein interaction databases

BioGridi216501. 2 interactions.

Structurei

3D structure databases

ProteinModelPortaliQ99LG0.
SMRiQ99LG0. Positions 22-427, 630-821.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini195 – 824630USP
Add
BLAST

Domaini

The UBP-type zinc finger binds 3 zinc ions that form a pair of cross-braced ring fingers encapsulated within a third zinc finger in the primary structure. It recognizes the C-terminal tail of free ubiquitin By similarity.UniRule annotation

Sequence similaritiesi

Contains 1 USP domain.

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiCOG5207.
GeneTreeiENSGT00750000117419.
HOGENOMiHOG000154755.
HOVERGENiHBG062704.
InParanoidiQ99LG0.
KOiK11844.
OMAiECSIQHC.
TreeFamiTF326075.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
HAMAPiMF_03062. UBP16.
InterProiIPR018200. Pept_C19ubi-hydrolase_C_CS.
IPR001394. Peptidase_C19_UCH.
IPR028889. UCH/PAN2.
IPR013083. Znf_RING/FYVE/PHD.
IPR001607. Znf_UBP.
[Graphical view]
PfamiPF00443. UCH. 1 hit.
PF02148. zf-UBP. 1 hit.
[Graphical view]
PROSITEiPS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
PS50271. ZF_UBP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q99LG0-1 [UniParc]FASTAAdd to Basket

« Hide

MGKKRTKGRS APDTVASESA EPVCRHLRKG LEQGNLKKAL VNVEWNICQD    50
CKTDNKVKDK PEEEAEDPSV WLCLKCGHQG CGRDSQEQHA LKHYTTPRSE 100
PHYLVLSLDN WSVWCYKCDE EVKYCSSNRL GQVVDYVRKQ AGVRTSKPAE 150
KNNGHIELEN KKLEKESKNE QEREKSENLA KETIPMDSAS QITVKGLSNL 200
GNTCFFNAVM QNLSQTPVLR ELLKEVKMSG TIVKIEPPDL ALTEPLEVNL 250
EPPGPLTLAM SQFLSEMQEN KKRVVTPKEL FSQVCKKATR FKGYQQQDSQ 300
ELLRYLLDGM RAEEHQRVSK GILKAFGNST EKLDEEVKNK VKDYEKKKAI 350
PSFVDRIFGG ELTSTIMCDE CRTVSLVHES FLDLSLPVLD DQSGKKSIND 400
KNVKMTMEEE DKDSEEEKDD SYMKSRSDLP SGTSKHLQKK AKKQAKKQAK 450
NQRRQQKIQE RFLHFNELCA TDYTEDNERE ADTALAGEVE VDTDSTHGSQ 500
EEATQIELSV NQKDLDGQES MIERTPDVQE SPEDLGVKSA NTESDLGIVT 550
PAPECPRDFN GAFLEERTSG ELDIINGLKN LNLNAAVDPD EINIEIPNDS 600
HSAPKVYEVM NEDPETAFCT LANREAFSTD ECSIQHCLYQ FTRNEKLQDA 650
NKLLCEVCSR RQCNGPKANI KGDRRHVYTN AKKQMLVSLA PPVLTLHLKR 700
FQQAGFNLRK VNKHIKFPEI LDLAPFCTLK CKNVAEESTR VLYSLYGVVE 750
HSGTMRSGHY TAYAKERTAS CHLSNLVLHG DIPQDCEMES TKGQWFHISD 800
THVQAVPITK VLNSQAYLLF YERIL 825
Length:825
Mass (Da):93,434
Last modified:December 11, 2013 - v2
Checksum:i08E8ED64D233461E
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti173 – 1731R → K in BAE35580. 1 Publication
Sequence conflicti173 – 1731R → K in AAH03278. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AK160038 mRNA. Translation: BAE35580.1.
AC154631 Genomic DNA. No translation available.
CH466521 Genomic DNA. Translation: EDK98336.1.
BC003278 mRNA. Translation: AAH03278.1.
BC004577 mRNA. Translation: AAH04577.1.
CCDSiCCDS28291.1.
RefSeqiNP_077220.2. NM_024258.2.
XP_006523163.1. XM_006523100.1.
UniGeneiMm.152941.

Genome annotation databases

EnsembliENSMUST00000026710; ENSMUSP00000026710; ENSMUSG00000025616.
GeneIDi74112.
KEGGimmu:74112.
UCSCiuc007zuk.2. mouse.
uc012ahp.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AK160038 mRNA. Translation: BAE35580.1 .
AC154631 Genomic DNA. No translation available.
CH466521 Genomic DNA. Translation: EDK98336.1 .
BC003278 mRNA. Translation: AAH03278.1 .
BC004577 mRNA. Translation: AAH04577.1 .
CCDSi CCDS28291.1.
RefSeqi NP_077220.2. NM_024258.2.
XP_006523163.1. XM_006523100.1.
UniGenei Mm.152941.

3D structure databases

ProteinModelPortali Q99LG0.
SMRi Q99LG0. Positions 22-427, 630-821.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 216501. 2 interactions.

Protein family/group databases

MEROPSi C19.050.

PTM databases

PhosphoSitei Q99LG0.

Proteomic databases

PaxDbi Q99LG0.
PRIDEi Q99LG0.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000026710 ; ENSMUSP00000026710 ; ENSMUSG00000025616 .
GeneIDi 74112.
KEGGi mmu:74112.
UCSCi uc007zuk.2. mouse.
uc012ahp.1. mouse.

Organism-specific databases

CTDi 10600.
MGIi MGI:1921362. Usp16.

Phylogenomic databases

eggNOGi COG5207.
GeneTreei ENSGT00750000117419.
HOGENOMi HOG000154755.
HOVERGENi HBG062704.
InParanoidi Q99LG0.
KOi K11844.
OMAi ECSIQHC.
TreeFami TF326075.

Miscellaneous databases

NextBioi 339808.
PROi Q99LG0.
SOURCEi Search...

Gene expression databases

Genevestigatori Q99LG0.

Family and domain databases

Gene3Di 3.30.40.10. 1 hit.
HAMAPi MF_03062. UBP16.
InterProi IPR018200. Pept_C19ubi-hydrolase_C_CS.
IPR001394. Peptidase_C19_UCH.
IPR028889. UCH/PAN2.
IPR013083. Znf_RING/FYVE/PHD.
IPR001607. Znf_UBP.
[Graphical view ]
Pfami PF00443. UCH. 1 hit.
PF02148. zf-UBP. 1 hit.
[Graphical view ]
PROSITEi PS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
PS50271. ZF_UBP. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  3. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Czech II and FVB/N.
    Tissue: Mammary tumor.
  5. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-520, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.
  6. "The Aurora B kinase and the Polycomb protein Ring1B combine to regulate active promoters in quiescent lymphocytes."
    Frangini A., Sjoberg M., Roman-Trufero M., Dharmalingam G., Haberle V., Bartke T., Lenhard B., Malumbres M., Vidal M., Dillon N.
    Mol. Cell 51:647-661(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, PHOSPHORYLATION.
  7. Cited for: UP-REGULATION IN DOWN SYNDROME MODELS.

Entry informationi

Entry nameiUBP16_MOUSE
AccessioniPrimary (citable) accession number: Q99LG0
Secondary accession number(s): G5E860, Q99KM0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: December 11, 2013
Last modified: September 3, 2014
This is version 105 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

Usp16 acts as a regulator of stem cell self-renewal and its overexpression contributes to somatic stem cell defects observed in Down syndrome models in mouse. Usp16 is triplicated in Ts65D Down syndrome mouse model and its overexpression leads to reduce the self-renewal of haematopoietic stem cells and the expansion of mammary epithelial cells, neural progenitors and fibroblasts. Defects are rescued by down-regulating Usp16 in Ts65D mice by short interfering RNAs (1 Publication).

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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