Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Ubiquitin carboxyl-terminal hydrolase 16

Gene

Usp16

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Specifically deubiquitinates 'Lys-120' of histone H2A (H2AK119Ub), a specific tag for epigenetic transcriptional repression, thereby acting as a coactivator. Deubiquitination of histone H2A is a prerequisite for subsequent phosphorylation at 'Ser-11' of histone H3 (H3S10ph), and is required for chromosome segregation when cells enter into mitosis. In resting B- and T-lymphocytes, phosphorylation by AURKB leads to enhance its activity, thereby maintaining transcription in resting lymphocytes (PubMed:24034696). Regulates Hox gene expression via histone H2A deubiquitination. Prefers nucleosomal substrates. Does not deubiquitinate histone H2B.1 Publication

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi24Zinc 1UniRule annotation1
Metal bindingi26Zinc 1UniRule annotation1
Metal bindingi48Zinc 2UniRule annotation1
Metal bindingi51Zinc 2UniRule annotation1
Metal bindingi73Zinc 3UniRule annotation1
Metal bindingi76Zinc 3UniRule annotation1
Metal bindingi81Zinc 2UniRule annotation1
Metal bindingi89Zinc 2UniRule annotation1
Metal bindingi93Zinc 3UniRule annotation1
Metal bindingi102Zinc 3UniRule annotation1
Metal bindingi115Zinc 1UniRule annotation1
Metal bindingi118Zinc 1UniRule annotation1
Active sitei204NucleophileUniRule annotation1
Active sitei759Proton acceptorUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri46 – 124UBP-typeUniRule annotationAdd BLAST79

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Activator, Chromatin regulator, Hydrolase, Protease, Thiol protease

Keywords - Biological processi

Cell cycle, Cell division, Mitosis, Transcription, Transcription regulation, Ubl conjugation pathway

Keywords - Ligandi

Metal-binding, Zinc

Protein family/group databases

MEROPSiC19.050.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin carboxyl-terminal hydrolase 16UniRule annotation (EC:3.4.19.12UniRule annotation)
Alternative name(s):
Deubiquitinating enzyme 16UniRule annotation
Ubiquitin thioesterase 16UniRule annotation
Ubiquitin-specific-processing protease 16UniRule annotation
Gene namesi
Name:Usp16
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 16

Organism-specific databases

MGIiMGI:1921362. Usp16.

Subcellular locationi

  • Nucleus UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003675031 – 825Ubiquitin carboxyl-terminal hydrolase 16Add BLAST825

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei188PhosphoserineBy similarity1
Modified residuei414PhosphoserineCombined sources1
Modified residuei520PhosphoserineCombined sources1
Modified residuei531PhosphoserineCombined sources1

Post-translational modificationi

Phosphorylated at the onset of mitosis and dephosphorylated during the metaphase/anaphase transition. Phosphorylation by AURKB enhances the deubiquitinase activity.UniRule annotation1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ99LG0.
MaxQBiQ99LG0.
PaxDbiQ99LG0.
PeptideAtlasiQ99LG0.
PRIDEiQ99LG0.

PTM databases

iPTMnetiQ99LG0.
PhosphoSitePlusiQ99LG0.

Expressioni

Gene expression databases

BgeeiENSMUSG00000025616.
ExpressionAtlasiQ99LG0. baseline and differential.
GenevisibleiQ99LG0. MM.

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

GO - Molecular functioni

Protein-protein interaction databases

BioGridi216501. 2 interactors.
STRINGi10090.ENSMUSP00000026710.

Structurei

3D structure databases

ProteinModelPortaliQ99LG0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini195 – 824USPAdd BLAST630

Domaini

The UBP-type zinc finger binds 3 zinc ions that form a pair of cross-braced ring fingers encapsulated within a third zinc finger in the primary structure. It recognizes the C-terminal tail of free ubiquitin.UniRule annotation

Sequence similaritiesi

Belongs to the peptidase C19 family. USP16 subfamily.UniRule annotation
Contains 1 UBP-type zinc finger.UniRule annotation
Contains 1 USP domain.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri46 – 124UBP-typeUniRule annotationAdd BLAST79

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiKOG1873. Eukaryota.
COG5560. LUCA.
GeneTreeiENSGT00860000133682.
HOGENOMiHOG000154755.
HOVERGENiHBG062704.
InParanoidiQ99LG0.
KOiK11844.
OMAiFEMQSTK.
OrthoDBiEOG091G01WT.
TreeFamiTF326075.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
HAMAPiMF_03062. UBP16. 1 hit.
InterProiIPR001394. Peptidase_C19_UCH.
IPR030849. UBP16.
IPR018200. USP_CS.
IPR028889. USP_dom.
IPR013083. Znf_RING/FYVE/PHD.
IPR001607. Znf_UBP.
[Graphical view]
PfamiPF00443. UCH. 1 hit.
PF02148. zf-UBP. 1 hit.
[Graphical view]
SMARTiSM00290. ZnF_UBP. 1 hit.
[Graphical view]
PROSITEiPS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
PS50271. ZF_UBP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q99LG0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGKKRTKGRS APDTVASESA EPVCRHLRKG LEQGNLKKAL VNVEWNICQD
60 70 80 90 100
CKTDNKVKDK PEEEAEDPSV WLCLKCGHQG CGRDSQEQHA LKHYTTPRSE
110 120 130 140 150
PHYLVLSLDN WSVWCYKCDE EVKYCSSNRL GQVVDYVRKQ AGVRTSKPAE
160 170 180 190 200
KNNGHIELEN KKLEKESKNE QEREKSENLA KETIPMDSAS QITVKGLSNL
210 220 230 240 250
GNTCFFNAVM QNLSQTPVLR ELLKEVKMSG TIVKIEPPDL ALTEPLEVNL
260 270 280 290 300
EPPGPLTLAM SQFLSEMQEN KKRVVTPKEL FSQVCKKATR FKGYQQQDSQ
310 320 330 340 350
ELLRYLLDGM RAEEHQRVSK GILKAFGNST EKLDEEVKNK VKDYEKKKAI
360 370 380 390 400
PSFVDRIFGG ELTSTIMCDE CRTVSLVHES FLDLSLPVLD DQSGKKSIND
410 420 430 440 450
KNVKMTMEEE DKDSEEEKDD SYMKSRSDLP SGTSKHLQKK AKKQAKKQAK
460 470 480 490 500
NQRRQQKIQE RFLHFNELCA TDYTEDNERE ADTALAGEVE VDTDSTHGSQ
510 520 530 540 550
EEATQIELSV NQKDLDGQES MIERTPDVQE SPEDLGVKSA NTESDLGIVT
560 570 580 590 600
PAPECPRDFN GAFLEERTSG ELDIINGLKN LNLNAAVDPD EINIEIPNDS
610 620 630 640 650
HSAPKVYEVM NEDPETAFCT LANREAFSTD ECSIQHCLYQ FTRNEKLQDA
660 670 680 690 700
NKLLCEVCSR RQCNGPKANI KGDRRHVYTN AKKQMLVSLA PPVLTLHLKR
710 720 730 740 750
FQQAGFNLRK VNKHIKFPEI LDLAPFCTLK CKNVAEESTR VLYSLYGVVE
760 770 780 790 800
HSGTMRSGHY TAYAKERTAS CHLSNLVLHG DIPQDCEMES TKGQWFHISD
810 820
THVQAVPITK VLNSQAYLLF YERIL
Length:825
Mass (Da):93,434
Last modified:December 11, 2013 - v2
Checksum:i08E8ED64D233461E
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti173R → K in BAE35580 (PubMed:16141072).Curated1
Sequence conflicti173R → K in AAH03278 (PubMed:15489334).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK160038 mRNA. Translation: BAE35580.1.
AC154631 Genomic DNA. No translation available.
CH466521 Genomic DNA. Translation: EDK98336.1.
BC003278 mRNA. Translation: AAH03278.1.
BC004577 mRNA. Translation: AAH04577.1.
CCDSiCCDS28291.1.
RefSeqiNP_077220.2. NM_024258.2.
XP_006523163.1. XM_006523100.2.
UniGeneiMm.152941.

Genome annotation databases

EnsembliENSMUST00000026710; ENSMUSP00000026710; ENSMUSG00000025616.
GeneIDi74112.
KEGGimmu:74112.
UCSCiuc007zuk.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK160038 mRNA. Translation: BAE35580.1.
AC154631 Genomic DNA. No translation available.
CH466521 Genomic DNA. Translation: EDK98336.1.
BC003278 mRNA. Translation: AAH03278.1.
BC004577 mRNA. Translation: AAH04577.1.
CCDSiCCDS28291.1.
RefSeqiNP_077220.2. NM_024258.2.
XP_006523163.1. XM_006523100.2.
UniGeneiMm.152941.

3D structure databases

ProteinModelPortaliQ99LG0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi216501. 2 interactors.
STRINGi10090.ENSMUSP00000026710.

Protein family/group databases

MEROPSiC19.050.

PTM databases

iPTMnetiQ99LG0.
PhosphoSitePlusiQ99LG0.

Proteomic databases

EPDiQ99LG0.
MaxQBiQ99LG0.
PaxDbiQ99LG0.
PeptideAtlasiQ99LG0.
PRIDEiQ99LG0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000026710; ENSMUSP00000026710; ENSMUSG00000025616.
GeneIDi74112.
KEGGimmu:74112.
UCSCiuc007zuk.2. mouse.

Organism-specific databases

CTDi10600.
MGIiMGI:1921362. Usp16.

Phylogenomic databases

eggNOGiKOG1873. Eukaryota.
COG5560. LUCA.
GeneTreeiENSGT00860000133682.
HOGENOMiHOG000154755.
HOVERGENiHBG062704.
InParanoidiQ99LG0.
KOiK11844.
OMAiFEMQSTK.
OrthoDBiEOG091G01WT.
TreeFamiTF326075.

Miscellaneous databases

PROiQ99LG0.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000025616.
ExpressionAtlasiQ99LG0. baseline and differential.
GenevisibleiQ99LG0. MM.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
HAMAPiMF_03062. UBP16. 1 hit.
InterProiIPR001394. Peptidase_C19_UCH.
IPR030849. UBP16.
IPR018200. USP_CS.
IPR028889. USP_dom.
IPR013083. Znf_RING/FYVE/PHD.
IPR001607. Znf_UBP.
[Graphical view]
PfamiPF00443. UCH. 1 hit.
PF02148. zf-UBP. 1 hit.
[Graphical view]
SMARTiSM00290. ZnF_UBP. 1 hit.
[Graphical view]
PROSITEiPS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
PS50271. ZF_UBP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiUBP16_MOUSE
AccessioniPrimary (citable) accession number: Q99LG0
Secondary accession number(s): G5E860, Q99KM0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: December 11, 2013
Last modified: November 30, 2016
This is version 129 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

Usp16 acts as a regulator of stem cell self-renewal and its overexpression contributes to somatic stem cell defects observed in Down syndrome models in mouse. Usp16 is triplicated in Ts65D Down syndrome mouse model and its overexpression leads to reduce the self-renewal of haematopoietic stem cells and the expansion of mammary epithelial cells, neural progenitors and fibroblasts. Defects are rescued by down-regulating Usp16 in Ts65D mice by short interfering RNAs (PubMed:24025767).1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.