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Protein

N(G),N(G)-dimethylarginine dimethylaminohydrolase 2

Gene

Ddah2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Hydrolyzes N(G),N(G)-dimethyl-L-arginine (ADMA) and N(G)-monomethyl-L-arginine (MMA) which act as inhibitors of NOS. Has therefore a role in the regulation of nitric oxide generation (By similarity).By similarity

Catalytic activityi

N(omega),N(omega)-dimethyl-L-arginine + H2O = dimethylamine + L-citrulline.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei171Proton donorBy similarity1
Active sitei276NucleophileSequence analysis1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Enzyme and pathway databases

BRENDAi3.5.3.18. 3474.
ReactomeiR-MMU-203615. eNOS activation.

Names & Taxonomyi

Protein namesi
Recommended name:
N(G),N(G)-dimethylarginine dimethylaminohydrolase 2 (EC:3.5.3.18)
Short name:
DDAH-2
Short name:
Dimethylarginine dimethylaminohydrolase 2
Alternative name(s):
DDAHII
Dimethylargininase-2
Gene namesi
Name:Ddah2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 17

Organism-specific databases

MGIiMGI:1859016. Ddah2.

Subcellular locationi

  • Cytoplasm By similarity
  • Mitochondrion By similarity

  • Note: Translocates from cytosol to mitochondrion upon IL-1beta stimulation in chondrocytes.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001711221 – 285N(G),N(G)-dimethylarginine dimethylaminohydrolase 2Add BLAST285

Proteomic databases

EPDiQ99LD8.
MaxQBiQ99LD8.
PaxDbiQ99LD8.
PeptideAtlasiQ99LD8.
PRIDEiQ99LD8.

2D gel databases

REPRODUCTION-2DPAGEIPI00336881.
UCD-2DPAGEQ99LD8.

PTM databases

iPTMnetiQ99LD8.
PhosphoSitePlusiQ99LD8.

Expressioni

Gene expression databases

BgeeiENSMUSG00000007039.
CleanExiMM_DDAH2.
ExpressionAtlasiQ99LD8. baseline and differential.
GenevisibleiQ99LD8. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000007255.

Structurei

3D structure databases

ProteinModelPortaliQ99LD8.
SMRiQ99LD8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the DDAH family.Curated

Phylogenomic databases

eggNOGiENOG410ISNS. Eukaryota.
ENOG4111YMV. LUCA.
GeneTreeiENSGT00390000009331.
HOGENOMiHOG000161035.
HOVERGENiHBG055937.
InParanoidiQ99LD8.
KOiK01482.
OMAiANCIYVR.
OrthoDBiEOG091G0M5S.
PhylomeDBiQ99LD8.
TreeFamiTF314737.

Family and domain databases

InterProiIPR033199. DDAH/AD.
IPR033202. DDAH2.
[Graphical view]
PANTHERiPTHR12737. PTHR12737. 1 hit.
PTHR12737:SF16. PTHR12737:SF16. 1 hit.

Sequencei

Sequence statusi: Complete.

Q99LD8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGTPGEGLGR CSHALIRGVP ESLASGEGAG AGLPALDLAK AQREHGVLGG
60 70 80 90 100
KLRQRLGLQL LELPPEESLP LGPLLGDTAV IQGDTALITR PWSPARRPEV
110 120 130 140 150
DGVRKALQDL GLRIVEMGDE NATLDGTDVL FTGREFFVGL SKWTNHRGAE
160 170 180 190 200
IVADTFRDFA VSTVPVSGSS HLRGLCGMGG PRTVVAGSSE AAQKAVRAMA
210 220 230 240 250
ALTDHPYASL TLPDDAASDC LFLRPGLPGA TPFLLHRGGG DLPNSQEALQ
260 270 280
KLSDVTLVPV SCSELEKAGA GLSSLCLVLS TRPHC
Length:285
Mass (Da):29,646
Last modified:June 1, 2001 - v1
Checksum:i3795D0BA2D314EE5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC003328 mRNA. Translation: AAH03328.1.
CCDSiCCDS50082.1.
RefSeqiNP_001177378.1. NM_001190449.1.
XP_006524675.1. XM_006524612.3.
UniGeneiMm.1457.

Genome annotation databases

EnsembliENSMUST00000007255; ENSMUSP00000007255; ENSMUSG00000007039.
GeneIDi51793.
KEGGimmu:51793.
UCSCiuc008cfi.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC003328 mRNA. Translation: AAH03328.1.
CCDSiCCDS50082.1.
RefSeqiNP_001177378.1. NM_001190449.1.
XP_006524675.1. XM_006524612.3.
UniGeneiMm.1457.

3D structure databases

ProteinModelPortaliQ99LD8.
SMRiQ99LD8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000007255.

PTM databases

iPTMnetiQ99LD8.
PhosphoSitePlusiQ99LD8.

2D gel databases

REPRODUCTION-2DPAGEIPI00336881.
UCD-2DPAGEQ99LD8.

Proteomic databases

EPDiQ99LD8.
MaxQBiQ99LD8.
PaxDbiQ99LD8.
PeptideAtlasiQ99LD8.
PRIDEiQ99LD8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000007255; ENSMUSP00000007255; ENSMUSG00000007039.
GeneIDi51793.
KEGGimmu:51793.
UCSCiuc008cfi.2. mouse.

Organism-specific databases

CTDi23564.
MGIiMGI:1859016. Ddah2.

Phylogenomic databases

eggNOGiENOG410ISNS. Eukaryota.
ENOG4111YMV. LUCA.
GeneTreeiENSGT00390000009331.
HOGENOMiHOG000161035.
HOVERGENiHBG055937.
InParanoidiQ99LD8.
KOiK01482.
OMAiANCIYVR.
OrthoDBiEOG091G0M5S.
PhylomeDBiQ99LD8.
TreeFamiTF314737.

Enzyme and pathway databases

BRENDAi3.5.3.18. 3474.
ReactomeiR-MMU-203615. eNOS activation.

Miscellaneous databases

PROiQ99LD8.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000007039.
CleanExiMM_DDAH2.
ExpressionAtlasiQ99LD8. baseline and differential.
GenevisibleiQ99LD8. MM.

Family and domain databases

InterProiIPR033199. DDAH/AD.
IPR033202. DDAH2.
[Graphical view]
PANTHERiPTHR12737. PTHR12737. 1 hit.
PTHR12737:SF16. PTHR12737:SF16. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiDDAH2_MOUSE
AccessioniPrimary (citable) accession number: Q99LD8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 15, 2004
Last sequence update: June 1, 2001
Last modified: November 2, 2016
This is version 111 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.