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Protein

Electron transfer flavoprotein subunit alpha, mitochondrial

Gene

Etfa

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Heterodimeric electron transfer flavoprotein that accepts electrons from several mitochondrial dehydrogenases, including acyl-CoA dehydrogenases, glutaryl-CoA and sarcosine dehydrogenase. It transfers the electrons to the main mitochondrial respiratory chain via ETF-ubiquinone oxidoreductase (ETF dehydrogenase). Required for normal mitochondrial fatty acid oxidation and normal amino acid metabolism.By similarity

Cofactori

FADBy similarityNote: Binds 1 FAD per dimer.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei223FADBy similarity1
Binding sitei248FADBy similarity1
Binding sitei300FADBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi263 – 266FADBy similarity4
Nucleotide bindingi281 – 286FADBy similarity6
Nucleotide bindingi318 – 319FADBy similarity2

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processElectron transport, Transport
LigandFAD, Flavoprotein

Enzyme and pathway databases

ReactomeiR-MMU-611105 Respiratory electron transport

Names & Taxonomyi

Protein namesi
Recommended name:
Electron transfer flavoprotein subunit alpha, mitochondrial
Short name:
Alpha-ETF
Gene namesi
Name:Etfa
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 9

Organism-specific databases

MGIiMGI:106092 Etfa

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 19MitochondrionSequence analysisAdd BLAST19
ChainiPRO_000000865220 – 333Electron transfer flavoprotein subunit alpha, mitochondrialAdd BLAST314

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei59N6-acetyllysine; alternateCombined sources1
Modified residuei59N6-succinyllysine; alternateCombined sources1
Modified residuei62N6-acetyllysineCombined sources1
Modified residuei69N6-acetyllysine; alternateCombined sources1
Modified residuei69N6-succinyllysine; alternateCombined sources1
Modified residuei75N6-acetyllysineCombined sources1
Modified residuei85N6-acetyllysine; alternateCombined sources1
Modified residuei85N6-succinyllysine; alternateCombined sources1
Modified residuei93PhosphothreonineCombined sources1
Modified residuei101N6-acetyllysineCombined sources1
Modified residuei139N6-acetyllysineCombined sources1
Modified residuei140PhosphoserineBy similarity1
Modified residuei158N6-acetyllysine; alternateCombined sources1
Modified residuei158N6-succinyllysine; alternateCombined sources1
Modified residuei164N6-acetyllysineCombined sources1
Modified residuei187N6-succinyllysineCombined sources1
Modified residuei203N6-acetyllysine; alternateCombined sources1
Modified residuei203N6-succinyllysine; alternateCombined sources1
Modified residuei216N6-succinyllysineCombined sources1
Modified residuei226N6-acetyllysine; alternateCombined sources1
Modified residuei226N6-succinyllysine; alternateCombined sources1
Modified residuei232N6-acetyllysine; alternateCombined sources1
Modified residuei232N6-succinyllysine; alternateCombined sources1
Modified residuei301N6-succinyllysineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ99LC5
MaxQBiQ99LC5
PaxDbiQ99LC5
PeptideAtlasiQ99LC5
PRIDEiQ99LC5

2D gel databases

REPRODUCTION-2DPAGEiQ99LC5
UCD-2DPAGEiQ99LC5

PTM databases

iPTMnetiQ99LC5
PhosphoSitePlusiQ99LC5
SwissPalmiQ99LC5

Expressioni

Gene expression databases

BgeeiENSMUSG00000032314
GenevisibleiQ99LC5 MM

Interactioni

Subunit structurei

Heterodimer composed of ETFA and ETFB. Identified in a complex that contains ETFA, ETFB and ETFRF1. Interaction with ETFRF1 promotes dissociation of the bound FAD and loss of electron transfer activity.By similarity

Protein-protein interaction databases

BioGridi225952, 2 interactors
IntActiQ99LC5, 3 interactors
MINTiQ99LC5
STRINGi10090.ENSMUSP00000034866

Structurei

3D structure databases

ProteinModelPortaliQ99LC5
SMRiQ99LC5
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni20 – 204Domain IBy similarityAdd BLAST185
Regioni205 – 333Domain IIBy similarityAdd BLAST129

Domaini

Domain I shares an identical polypeptide fold with the beta subunit ETFB though there is no sequence similarity.By similarity

Sequence similaritiesi

Belongs to the ETF alpha-subunit/FixB family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG3954 Eukaryota
COG2025 LUCA
GeneTreeiENSGT00390000013422
HOGENOMiHOG000247865
HOVERGENiHBG002317
InParanoidiQ99LC5
KOiK03522
OMAiSQFKFTH
OrthoDBiEOG091G0EQM
PhylomeDBiQ99LC5
TreeFamiTF105763

Family and domain databases

CDDicd01715 ETF_alpha, 1 hit
Gene3Di3.40.50.620, 1 hit
InterProiView protein in InterPro
IPR029035 DHS-like_NAD/FAD-binding_dom
IPR014730 ETF_a/b_N
IPR001308 ETF_a/FixB
IPR033947 ETF_alpha_N
IPR014731 ETF_asu_C
IPR018206 ETF_asu_C_CS
IPR014729 Rossmann-like_a/b/a_fold
PfamiView protein in Pfam
PF01012 ETF, 1 hit
PF00766 ETF_alpha, 1 hit
PIRSFiPIRSF000089 Electra_flavoP_a, 1 hit
SMARTiView protein in SMART
SM00893 ETF, 1 hit
SUPFAMiSSF52467 SSF52467, 1 hit
PROSITEiView protein in PROSITE
PS00696 ETF_ALPHA, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q99LC5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFRAAAPGQL RRAASLLRFQ STLVIAEHAN DSLAPITLNT ITAAGRLGGE
60 70 80 90 100
VSCLVAGTKC DKVVQDLCKV AGVAKVLVAQ HDAYKGLLPE ELTPLILETQ
110 120 130 140 150
KQFSYTHICA GASAFGKNLL PRVAAKLNVA PVSDIIEIKS PDTFVRTIYA
160 170 180 190 200
GNALCTVKCD EKVKVFSVRG TSFEAAATSG GSASSEKAPS SSSVGISEWL
210 220 230 240 250
DQKLTKSDRP ELTGAKVVVS GGRGLKSGEN FKLLYDLADQ LHAAVGASRA
260 270 280 290 300
AVDAGFVPND MQVGQTGKIV APELYIAVGI SGAIQHLAGM KDSKTIVAIN
310 320 330
KDPEAPIFQV ADYGIVADLF KVVPEMTEIL KKK
Length:333
Mass (Da):35,009
Last modified:May 1, 2007 - v2
Checksum:i635F34ADCDA254C0
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti20Q → R in BAE40260 (PubMed:16141072).Curated1
Sequence conflicti85K → E in BAE21705 (PubMed:16141072).Curated1
Sequence conflicti209R → P in BAC25758 (PubMed:16141072).Curated1
Sequence conflicti288A → T in AAH03432 (PubMed:15489334).Curated1
Sequence conflicti299I → V in BAC28046 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK028118 mRNA Translation: BAC25758.1
AK032830 mRNA Translation: BAC28046.1
AK133525 mRNA Translation: BAE21705.1
AK167572 mRNA Translation: BAE39635.1
AK168321 mRNA Translation: BAE40260.1
BC003432 mRNA Translation: AAH03432.1
BC096645 mRNA Translation: AAH96645.1
CCDSiCCDS23204.1
RefSeqiNP_663590.3, NM_145615.4
UniGeneiMm.290853

Genome annotation databases

EnsembliENSMUST00000034866; ENSMUSP00000034866; ENSMUSG00000032314
GeneIDi110842
KEGGimmu:110842
UCSCiuc009psl.2 mouse

Similar proteinsi

Entry informationi

Entry nameiETFA_MOUSE
AccessioniPrimary (citable) accession number: Q99LC5
Secondary accession number(s): Q3THD7
, Q3V000, Q4V9X5, Q8BMD3, Q8BMU7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 11, 2002
Last sequence update: May 1, 2007
Last modified: March 28, 2018
This is version 140 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome
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Main funding by: National Institutes of Health