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Protein

NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial

Gene

Ndufa10

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.1 Publication

Cofactori

FADNote: Binds 1 FAD per subunit.

GO - Molecular functioni

  • NADH dehydrogenase (ubiquinone) activity Source: MGI

GO - Biological processi

Keywordsi

Biological processElectron transport, Respiratory chain, Transport
LigandFAD, Flavoprotein

Enzyme and pathway databases

ReactomeiR-MMU-611105 Respiratory electron transport
R-MMU-6799198 Complex I biogenesis

Names & Taxonomyi

Protein namesi
Recommended name:
NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial
Alternative name(s):
Complex I-42kD
Short name:
CI-42kD
NADH-ubiquinone oxidoreductase 42 kDa subunit
Gene namesi
Name:Ndufa10
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:1914523 Ndufa10

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Mitochondrion

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi250S → A: Leads to mitochondrial depolarization. 1 Publication1
Mutagenesisi250S → D: Phosphomimetic mutant; able to rescue mitochondrial depolarization in a Pink1 mutant background. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 35MitochondrionBy similarityAdd BLAST35
ChainiPRO_000001998936 – 355NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrialAdd BLAST320

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei122N6-acetyllysine; alternateCombined sources1
Modified residuei122N6-succinyllysine; alternateCombined sources1
Modified residuei250Phosphoserine; by PINK11 Publication1
Modified residuei285N6-succinyllysineCombined sources1

Post-translational modificationi

Phosphorylation at Ser-250 by PINK1 is required for the binding and/or reduction of the complex I substrate ubiquinone.1 Publication
Acetylation of Lys-242 is observed in liver mitochondria from fasted mice but not from fed mice.

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ99LC3
MaxQBiQ99LC3
PaxDbiQ99LC3
PeptideAtlasiQ99LC3
PRIDEiQ99LC3

2D gel databases

REPRODUCTION-2DPAGEiQ99LC3

PTM databases

iPTMnetiQ99LC3
PhosphoSitePlusiQ99LC3

Expressioni

Gene expression databases

BgeeiENSMUSG00000026260
ExpressionAtlasiQ99LC3 baseline and differential
GenevisibleiQ99LC3 MM

Interactioni

Subunit structurei

Complex I is composed of 45 different subunits. This a component of the hydrophobic protein fraction.By similarity

Protein-protein interaction databases

BioGridi212064, 1 interactor
CORUMiQ99LC3
IntActiQ99LC3, 6 interactors
MINTiQ99LC3
STRINGi10090.ENSMUSP00000027478

Structurei

3D structure databases

ProteinModelPortaliQ99LC3
SMRiQ99LC3
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the complex I NDUFA10 subunit family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG3877 Eukaryota
ENOG41101CN LUCA
GeneTreeiENSGT00390000016151
HOGENOMiHOG000267026
HOVERGENiHBG002308
InParanoidiQ99LC3
KOiK03954
OMAiKPHLVIY
OrthoDBiEOG091G0WZ0
PhylomeDBiQ99LC3
TreeFamiTF314616

Family and domain databases

CDDicd02030 NDUO42, 1 hit
InterProiView protein in InterPro
IPR031314 DNK_dom
IPR015828 NDUFA10
IPR027417 P-loop_NTPase
PfamiView protein in Pfam
PF01712 dNK, 1 hit
PIRSFiPIRSF000543 NADH_UQ_42KD, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q99LC3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALRLLRLVP ASAPARGLAA GAQRVGRIHT SVHCKLRYGL LAAILGDKTT
60 70 80 90 100
KKLHEYSRVI TVDGNICSGK NKLAKEIAQQ LGMKHYPEAG IQYSSTTTGD
110 120 130 140 150
GRPLDIEFSG SCSLEKFYDD PKSNDGNSYR LQSWLYASRL LQYADALEHL
160 170 180 190 200
LSTGQGVVLE RSIYSDFVFL EAMYNQGYIR KQCVDHYNEI KRLTLPEYLP
210 220 230 240 250
PHAVIYIDVP VPEVQSRIQK KGDPHEMKVT SAYLQDIENA YKKTFLPKMS
260 270 280 290 300
EMCEVLVYDS WEAEDPTKVV EDIEYLKYNK GPWLKQDDWT FHYLRMLVQD
310 320 330 340 350
KTEVLNYTTI PVYLPEITIG AHQGSRIYNS FRELPGRKYA PGYNAEVGDK

WIWLK
Length:355
Mass (Da):40,603
Last modified:June 1, 2001 - v1
Checksum:i75364533B079A688
GO

Sequence cautioni

The sequence BAB23961 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti191K → E in BAB23961 (PubMed:16141072).Curated1
Sequence conflicti266P → T in BAC32674 (PubMed:16141072).Curated1
Sequence conflicti294L → S in BAB23961 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC003439 mRNA Translation: AAH03439.1
AK005339 mRNA Translation: BAB23961.2 Different initiation.
AK145979 mRNA Translation: BAE26801.1
AK046292 mRNA Translation: BAC32674.1
CCDSiCCDS15167.1
RefSeqiNP_077159.1, NM_024197.1
UniGeneiMm.248778

Genome annotation databases

EnsembliENSMUST00000027478; ENSMUSP00000027478; ENSMUSG00000026260
GeneIDi67273
KEGGimmu:67273
UCSCiuc007cbh.1 mouse

Similar proteinsi

Entry informationi

Entry nameiNDUAA_MOUSE
AccessioniPrimary (citable) accession number: Q99LC3
Secondary accession number(s): Q3UKK0, Q8BL57, Q9CW21
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 27, 2003
Last sequence update: June 1, 2001
Last modified: February 28, 2018
This is version 138 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome
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Main funding by: National Institutes of Health