ID   SARDH_MOUSE             Reviewed;         919 AA.
AC   Q99LB7;
DT   13-SEP-2004, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2001, sequence version 1.
DT   16-JUN-2009, entry version 53.
DE   RecName: Full=Sarcosine dehydrogenase, mitochondrial;
DE            Short=SarDH;
DE            EC=1.5.99.1;
DE   Flags: Precursor;
GN   Name=Sardh;
OS   Mus musculus (Mouse).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi;
OC   Muroidea; Muridae; Murinae; Mus.
OX   NCBI_TaxID=10090;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=FVB/N; TISSUE=Mammary tumor;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA
RT   project: the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [2]
RP   ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-885, AND MASS SPECTROMETRY.
RC   TISSUE=Liver;
RX   PubMed=16916647; DOI=10.1016/j.molcel.2006.06.026;
RA   Kim S.C., Sprung R., Chen Y., Xu Y., Ball H., Pei J., Cheng T.,
RA   Kho Y., Xiao H., Xiao L., Grishin N.V., White M., Yang X.-J., Zhao Y.;
RT   "Substrate and functional diversity of lysine acetylation revealed by
RT   a proteomics survey.";
RL   Mol. Cell 23:607-618(2006).
CC   -!- CATALYTIC ACTIVITY: Sarcosine + acceptor + H(2)O = glycine +
CC       formaldehyde + reduced acceptor.
CC   -!- COFACTOR: Binds 1 FAD covalently per monomer (By similarity).
CC   -!- PATHWAY: Amine and polyamine degradation; sarcosine degradation;
CC       formaldehyde and glycine from sarcosine: step 1/1.
CC   -!- SUBCELLULAR LOCATION: Mitochondrion matrix (By similarity).
CC   -!- SIMILARITY: Belongs to the gcvT family.
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DR   EMBL; BC003456; AAH03456.1; -; mRNA.
DR   IPI; IPI00136213; -.
DR   RefSeq; NP_619606.1; -.
DR   UniGene; Mm.278467; -.
DR   PhosphoSite; Q99LB7; -.
DR   Ensembl; ENSMUSG00000009614; Mus musculus.
DR   GeneID; 192166; -.
DR   KEGG; mmu:192166; -.
DR   MGI; MGI:2183102; Sardh.
DR   HOGENOM; Q99LB7; -.
DR   HOVERGEN; Q99LB7; -.
DR   OMA; Q99LB7; EAIWRNG.
DR   BRENDA; 1.5.99.1; 244.
DR   NextBio; 371164; -.
DR   ArrayExpress; Q99LB7; -.
DR   Bgee; Q99LB7; -.
DR   CleanEx; MM_SARDH; -.
DR   GermOnline; ENSMUSG00000009614; Mus musculus.
DR   GO; GO:0005759; C:mitochondrial matrix; IEA:UniProtKB-SubCell.
DR   GO; GO:0004047; F:aminomethyltransferase activity; IEA:InterPro.
DR   GO; GO:0008480; F:sarcosine dehydrogenase activity; IEA:EC.
DR   GO; GO:0006546; P:glycine catabolic process; IEA:InterPro.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   InterPro; IPR006076; FAD-dep_OxRdtase.
DR   InterPro; IPR013977; GCV_T_C.
DR   InterPro; IPR006222; GCV_T_N.
DR   Pfam; PF01266; DAO; 1.
DR   Pfam; PF01571; GCV_T; 1.
DR   Pfam; PF08669; GCV_T_C; 1.
PE   1: Evidence at protein level;
KW   Acetylation; FAD; Flavoprotein; Mitochondrion; Oxidoreductase;
KW   Phosphoprotein; Transit peptide.
FT   TRANSIT       1      ?       Mitochondrion (Potential).
FT   CHAIN         ?    919       Sarcosine dehydrogenase, mitochondrial.
FT                                /FTId=PRO_0000010771.
FT   MOD_RES     109    109       Tele-8alpha-FAD histidine (By
FT                                similarity).
FT   MOD_RES     778    778       Phosphotyrosine (By similarity).
FT   MOD_RES     885    885       N6-acetyllysine.
SQ   SEQUENCE   919 AA;  101682 MW;  4908ACB540C9D305 CRC64;
     MASLSRVLRV AATCPRGRAA WNLGLQPLAT EARPTTEKSV PYQRTLKEEA QGASVVPQGP
     SQPLPSTANV VVIGGGSLGC QTLYHLAKLG VGGAVLLERE RLTSGTTWHT AGLLWQLRPS
     DVEVELLAHT RQVVSRDLEE ETGLHTGWIQ NGGLFIASNQ QRLNEYKRLM SLGKAYGIES
     HVLSPAETKS LYPLMNVDDL YGTLYVPQDG TMDPAGTCTT LTRAAVARGA QVIENCAVTG
     IRVRTDDFGV RRVAAVETEH GSIQTPCVVN CAGVWASKVG RMAGVKVPLV AMHHAYVVTE
     RIEGIQNMPN VRDHDASVYL RLQGDALSVG GYEANPIFWE EVSDKFAFGL FDLDWDVFTQ
     HIEGAINRVP VLEKTGIKST VCGPESFTPD HKPLMGEAPE LRGFFLGCGF NSAGMMLGGG
     CGQELAHWIV HGRPEKDMYS YDIRRFHHSL TDHTRWIRER SHESYAKNYS VVFPHDEPLA
     GRNMRRDPLH EELLGQGCVF QERQGWERPG WFNPQETAQV LDYDYYGAYG NQAHKDYTYS
     RLLGDEYTFD FPPHHHMIQK ECLACRGAAA VFNMSYFGKF YLLGVDARKA ADWLFSADVN
     RPPGSTVYTC MLNQRGGTES DLTVSRLAPG TQASPLVPAF EGDCYYLAVG GAVAQHNWSH
     INTVLQDQEF RCQLMDSSED LGMLSIQGPA SRDILQDVLD ADLSNEAFPF STHQLVRAAG
     HLVRAIRLSF VGELGWELHV PRASCLPVYR AVMAAGARHG LVNAGYRAID SLSIEKGYRH
     WHADLRPDDS PLEAGLAFTC KLKTSVPFLG REALEKQRAT GLRRRLICLT VEEEVPMFGL
     EAIWRNGQVV GHVRRADFGF TVNKTIAYGY IRDPSGGPVS LDFVKNGEYA LERMGVTYAA
     QVHLKSPFDP DNKRVKGIY
//