Reviewed, UniProtKB/Swiss-Prot Q99LB7 (SARDH_MOUSE)

Names and origin

Protein names

Recommended name:
    Sarcosine dehydrogenase, mitochondrial
      Short name=SarDH
    EC=1.5.99.1

Gene names

Name:

Sardh

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	919 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	Sarcosine + acceptor + H2O = glycine + formaldehyde + reduced acceptor.
Cofactor	Binds 1 FAD covalently per monomer By similarity.
Pathway	Amine and polyamine degradation; sarcosine degradation; formaldehyde and glycine from sarcosine: step 1/1.
Subcellular location	Mitochondrion matrix By similarity.
Sequence similarities	Belongs to the gcvT family.

Ontologies

Keywords
Cellular component	Mitochondrion
Domain	Transit peptide
Ligand	FAD Flavoprotein
Molecular function	Oxidoreductase
PTM	Acetylation Phosphoprotein
Gene Ontology (GO)
Biological process	glycine catabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	mitochondrial matrix Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	aminomethyltransferase activity Inferred from electronic annotation. Source: InterPro sarcosine dehydrogenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – ?		Mitochondrion Potential
	Chain	? – 919		Sarcosine dehydrogenase, mitochondrial		PRO_0000010771
Amino acid modifications
	Modified residue	109	1	Tele-8alpha-FAD histidine By similarity
	Modified residue	778	1	Phosphotyrosine By similarity
	Modified residue	885	1	N6-acetyllysine Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q99LB7-1 [UniParc]. Last modified June 1, 2001. Version 1. Checksum: 4908ACB540C9D305	FASTA	919	101,682
	10 20 30 40 50 60 MASLSRVLRV AATCPRGRAA WNLGLQPLAT EARPTTEKSV PYQRTLKEEA QGASVVPQGP 70 80 90 100 110 120 SQPLPSTANV VVIGGGSLGC QTLYHLAKLG VGGAVLLERE RLTSGTTWHT AGLLWQLRPS 130 140 150 160 170 180 DVEVELLAHT RQVVSRDLEE ETGLHTGWIQ NGGLFIASNQ QRLNEYKRLM SLGKAYGIES 190 200 210 220 230 240 HVLSPAETKS LYPLMNVDDL YGTLYVPQDG TMDPAGTCTT LTRAAVARGA QVIENCAVTG 250 260 270 280 290 300 IRVRTDDFGV RRVAAVETEH GSIQTPCVVN CAGVWASKVG RMAGVKVPLV AMHHAYVVTE 310 320 330 340 350 360 RIEGIQNMPN VRDHDASVYL RLQGDALSVG GYEANPIFWE EVSDKFAFGL FDLDWDVFTQ 370 380 390 400 410 420 HIEGAINRVP VLEKTGIKST VCGPESFTPD HKPLMGEAPE LRGFFLGCGF NSAGMMLGGG 430 440 450 460 470 480 CGQELAHWIV HGRPEKDMYS YDIRRFHHSL TDHTRWIRER SHESYAKNYS VVFPHDEPLA 490 500 510 520 530 540 GRNMRRDPLH EELLGQGCVF QERQGWERPG WFNPQETAQV LDYDYYGAYG NQAHKDYTYS 550 560 570 580 590 600 RLLGDEYTFD FPPHHHMIQK ECLACRGAAA VFNMSYFGKF YLLGVDARKA ADWLFSADVN 610 620 630 640 650 660 RPPGSTVYTC MLNQRGGTES DLTVSRLAPG TQASPLVPAF EGDCYYLAVG GAVAQHNWSH 670 680 690 700 710 720 INTVLQDQEF RCQLMDSSED LGMLSIQGPA SRDILQDVLD ADLSNEAFPF STHQLVRAAG 730 740 750 760 770 780 HLVRAIRLSF VGELGWELHV PRASCLPVYR AVMAAGARHG LVNAGYRAID SLSIEKGYRH 790 800 810 820 830 840 WHADLRPDDS PLEAGLAFTC KLKTSVPFLG REALEKQRAT GLRRRLICLT VEEEVPMFGL 850 860 870 880 890 900 EAIWRNGQVV GHVRRADFGF TVNKTIAYGY IRDPSGGPVS LDFVKNGEYA LERMGVTYAA 910 QVHLKSPFDP DNKRVKGIY

References

[1]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: FVB/N. Tissue: Mammary tumor.
[2]	"Substrate and functional diversity of lysine acetylation revealed by a proteomics survey." Kim S.C., Sprung R., Chen Y., Xu Y., Ball H., Pei J., Cheng T., Kho Y., Xiao H., Xiao L., Grishin N.V., White M., Yang X.-J., Zhao Y. Mol. Cell 23:607-618(2006) [PubMed: 16916647] [Abstract] Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-885, MASS SPECTROMETRY. Tissue: Liver.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BC003456 mRNA. Translation: AAH03456.1.
IPI	IPI00136213.
RefSeq	NP_619606.1.
UniGene	Mm.278467
3D structure databases
ModBase	Search...
PTM databases
PhosphoSite	Q99LB7.
Genome annotation databases
Ensembl	ENSMUSG00000009614. Mus musculus. [Contig view]
GeneID	192166.
KEGG	mmu:192166.
Organism-specific databases
MGI	MGI:2183102. Sardh.
Phylogenomic databases
HOGENOM	Q99LB7.
HOVERGEN	Q99LB7.
OMA	Q99LB7. EAIWRNG.
Enzyme and pathway databases
BRENDA	1.5.99.1. 244.
Gene expression databases
ArrayExpress	Q99LB7.
Bgee	Q99LB7.
CleanEx	MM_SARDH.
GermOnline	ENSMUSG00000009614. Mus musculus.
Family and domain databases
InterPro	IPR006076. FAD-dep_OxRdtase. IPR013977. GCV_T_C. IPR006222. GCV_T_N. [Graphical view]
Pfam	PF01266. DAO. 1 hit. PF01571. GCV_T. 1 hit. PF08669. GCV_T_C. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	371164.
SOURCE	Search...

Entry information

Entry name

SARDH_MOUSE

Accession

Primary (citable) accession number: Q99LB7

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 13, 2004
Last sequence update:	June 1, 2001
Last modified:	June 16, 2009
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)