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Protein

Sarcosine dehydrogenase, mitochondrial

Gene

Sardh

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Sarcosine + H2O + electron-transfer flavoprotein = glycine + formaldehyde + reduced electron-transfer flavoprotein.

Cofactori

FADBy similarityNote: Binds 1 FAD covalently per monomer.By similarity

Pathwayi: sarcosine degradation

This protein is involved in step 1 of the subpathway that synthesizes formaldehyde and glycine from sarcosine.
Proteins known to be involved in this subpathway in this organism are:
  1. Sarcosine dehydrogenase, mitochondrial (Sardh)
This subpathway is part of the pathway sarcosine degradation, which is itself part of Amine and polyamine degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes formaldehyde and glycine from sarcosine, the pathway sarcosine degradation and in Amine and polyamine degradation.

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

FAD, Flavoprotein

Enzyme and pathway databases

ReactomeiR-MMU-6798163. Choline catabolism.
UniPathwayiUPA00292; UER00398.

Names & Taxonomyi

Protein namesi
Recommended name:
Sarcosine dehydrogenase, mitochondrial (EC:1.5.8.3)
Short name:
SarDH
Gene namesi
Name:Sardh
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:2183102. Sardh.

Subcellular locationi

GO - Cellular componenti

  • mitochondrial matrix Source: UniProtKB-SubCell
  • mitochondrion Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 22MitochondrionSequence analysisAdd BLAST22
ChainiPRO_000001077123 – 919Sarcosine dehydrogenase, mitochondrialAdd BLAST897

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei38N6-succinyllysineCombined sources1
Modified residuei109Tele-8alpha-FAD histidineBy similarity1
Modified residuei174N6-acetyllysine; alternateCombined sources1
Modified residuei174N6-succinyllysine; alternateCombined sources1
Modified residuei278N6-succinyllysineCombined sources1
Modified residuei378N6-succinyllysineCombined sources1
Modified residuei392N6-succinyllysineCombined sources1
Modified residuei535N6-succinyllysineCombined sources1
Modified residuei560N6-acetyllysineCombined sources1
Modified residuei776N6-acetyllysineCombined sources1
Modified residuei778PhosphotyrosineBy similarity1
Modified residuei803N6-acetyllysine; alternateCombined sources1
Modified residuei803N6-succinyllysine; alternateCombined sources1
Modified residuei885N6-acetyllysine; alternateCombined sources1
Modified residuei885N6-succinyllysine; alternateCombined sources1
Modified residuei905N6-acetyllysine; alternateCombined sources1
Modified residuei905N6-succinyllysine; alternateCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiQ99LB7.
PaxDbiQ99LB7.
PRIDEiQ99LB7.

PTM databases

iPTMnetiQ99LB7.
PhosphoSitePlusiQ99LB7.

Expressioni

Gene expression databases

BgeeiENSMUSG00000009614.
CleanExiMM_SARDH.
ExpressionAtlasiQ99LB7. baseline and differential.
GenevisibleiQ99LB7. MM.

Interactioni

Protein-protein interaction databases

BioGridi228656. 1 interactor.
IntActiQ99LB7. 3 interactors.
MINTiMINT-1839313.
STRINGi10090.ENSMUSP00000099950.

Structurei

3D structure databases

ProteinModelPortaliQ99LB7.
SMRiQ99LB7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the GcvT family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG2844. Eukaryota.
COG0404. LUCA.
GeneTreeiENSGT00530000063120.
HOGENOMiHOG000251716.
HOVERGENiHBG002326.
InParanoidiQ99LB7.
KOiK00314.
OMAiPMKHAYI.
OrthoDBiEOG091G01QG.
PhylomeDBiQ99LB7.
TreeFamiTF314735.

Family and domain databases

Gene3Di2.40.30.110. 1 hit.
3.30.1360.120. 3 hits.
3.50.50.60. 3 hits.
InterProiIPR006076. FAD-dep_OxRdtase.
IPR023753. FAD/NAD-binding_dom.
IPR032503. FAO_M.
IPR013977. GCV_T_C.
IPR006222. GCV_T_N.
IPR029043. GcvT/YgfZ_C.
IPR027266. TrmE/GcvT_dom1.
[Graphical view]
PfamiPF01266. DAO. 1 hit.
PF16350. FAO_M. 1 hit.
PF01571. GCV_T. 1 hit.
PF08669. GCV_T_C. 1 hit.
[Graphical view]
SUPFAMiSSF101790. SSF101790. 1 hit.
SSF51905. SSF51905. 2 hits.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q99LB7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASLSRVLRV AATCPRGRAA WNLGLQPLAT EARPTTEKSV PYQRTLKEEA
60 70 80 90 100
QGASVVPQGP SQPLPSTANV VVIGGGSLGC QTLYHLAKLG VGGAVLLERE
110 120 130 140 150
RLTSGTTWHT AGLLWQLRPS DVEVELLAHT RQVVSRDLEE ETGLHTGWIQ
160 170 180 190 200
NGGLFIASNQ QRLNEYKRLM SLGKAYGIES HVLSPAETKS LYPLMNVDDL
210 220 230 240 250
YGTLYVPQDG TMDPAGTCTT LTRAAVARGA QVIENCAVTG IRVRTDDFGV
260 270 280 290 300
RRVAAVETEH GSIQTPCVVN CAGVWASKVG RMAGVKVPLV AMHHAYVVTE
310 320 330 340 350
RIEGIQNMPN VRDHDASVYL RLQGDALSVG GYEANPIFWE EVSDKFAFGL
360 370 380 390 400
FDLDWDVFTQ HIEGAINRVP VLEKTGIKST VCGPESFTPD HKPLMGEAPE
410 420 430 440 450
LRGFFLGCGF NSAGMMLGGG CGQELAHWIV HGRPEKDMYS YDIRRFHHSL
460 470 480 490 500
TDHTRWIRER SHESYAKNYS VVFPHDEPLA GRNMRRDPLH EELLGQGCVF
510 520 530 540 550
QERQGWERPG WFNPQETAQV LDYDYYGAYG NQAHKDYTYS RLLGDEYTFD
560 570 580 590 600
FPPHHHMIQK ECLACRGAAA VFNMSYFGKF YLLGVDARKA ADWLFSADVN
610 620 630 640 650
RPPGSTVYTC MLNQRGGTES DLTVSRLAPG TQASPLVPAF EGDCYYLAVG
660 670 680 690 700
GAVAQHNWSH INTVLQDQEF RCQLMDSSED LGMLSIQGPA SRDILQDVLD
710 720 730 740 750
ADLSNEAFPF STHQLVRAAG HLVRAIRLSF VGELGWELHV PRASCLPVYR
760 770 780 790 800
AVMAAGARHG LVNAGYRAID SLSIEKGYRH WHADLRPDDS PLEAGLAFTC
810 820 830 840 850
KLKTSVPFLG REALEKQRAT GLRRRLICLT VEEEVPMFGL EAIWRNGQVV
860 870 880 890 900
GHVRRADFGF TVNKTIAYGY IRDPSGGPVS LDFVKNGEYA LERMGVTYAA
910
QVHLKSPFDP DNKRVKGIY
Length:919
Mass (Da):101,682
Last modified:June 1, 2001 - v1
Checksum:i4908ACB540C9D305
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC003456 mRNA. Translation: AAH03456.1.
CCDSiCCDS15826.1.
RefSeqiNP_619606.1. NM_138665.2.
XP_006497851.1. XM_006497788.3.
XP_006497852.1. XM_006497789.3.
UniGeneiMm.278467.

Genome annotation databases

EnsembliENSMUST00000102886; ENSMUSP00000099950; ENSMUSG00000009614.
GeneIDi192166.
KEGGimmu:192166.
UCSCiuc008ixg.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC003456 mRNA. Translation: AAH03456.1.
CCDSiCCDS15826.1.
RefSeqiNP_619606.1. NM_138665.2.
XP_006497851.1. XM_006497788.3.
XP_006497852.1. XM_006497789.3.
UniGeneiMm.278467.

3D structure databases

ProteinModelPortaliQ99LB7.
SMRiQ99LB7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi228656. 1 interactor.
IntActiQ99LB7. 3 interactors.
MINTiMINT-1839313.
STRINGi10090.ENSMUSP00000099950.

PTM databases

iPTMnetiQ99LB7.
PhosphoSitePlusiQ99LB7.

Proteomic databases

MaxQBiQ99LB7.
PaxDbiQ99LB7.
PRIDEiQ99LB7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000102886; ENSMUSP00000099950; ENSMUSG00000009614.
GeneIDi192166.
KEGGimmu:192166.
UCSCiuc008ixg.2. mouse.

Organism-specific databases

CTDi1757.
MGIiMGI:2183102. Sardh.

Phylogenomic databases

eggNOGiKOG2844. Eukaryota.
COG0404. LUCA.
GeneTreeiENSGT00530000063120.
HOGENOMiHOG000251716.
HOVERGENiHBG002326.
InParanoidiQ99LB7.
KOiK00314.
OMAiPMKHAYI.
OrthoDBiEOG091G01QG.
PhylomeDBiQ99LB7.
TreeFamiTF314735.

Enzyme and pathway databases

UniPathwayiUPA00292; UER00398.
ReactomeiR-MMU-6798163. Choline catabolism.

Miscellaneous databases

PROiQ99LB7.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000009614.
CleanExiMM_SARDH.
ExpressionAtlasiQ99LB7. baseline and differential.
GenevisibleiQ99LB7. MM.

Family and domain databases

Gene3Di2.40.30.110. 1 hit.
3.30.1360.120. 3 hits.
3.50.50.60. 3 hits.
InterProiIPR006076. FAD-dep_OxRdtase.
IPR023753. FAD/NAD-binding_dom.
IPR032503. FAO_M.
IPR013977. GCV_T_C.
IPR006222. GCV_T_N.
IPR029043. GcvT/YgfZ_C.
IPR027266. TrmE/GcvT_dom1.
[Graphical view]
PfamiPF01266. DAO. 1 hit.
PF16350. FAO_M. 1 hit.
PF01571. GCV_T. 1 hit.
PF08669. GCV_T_C. 1 hit.
[Graphical view]
SUPFAMiSSF101790. SSF101790. 1 hit.
SSF51905. SSF51905. 2 hits.
ProtoNetiSearch...

Entry informationi

Entry nameiSARDH_MOUSE
AccessioniPrimary (citable) accession number: Q99LB7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 13, 2004
Last sequence update: June 1, 2001
Last modified: November 2, 2016
This is version 115 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.