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Protein

Methionine adenosyltransferase 2 subunit beta

Gene

Mat2b

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Regulatory subunit of S-adenosylmethionine synthetase 2, an enzyme that catalyzes the formation of S-adenosylmethionine from methionine and ATP. Regulates MAT2A catalytic activity by changing its kinetic properties, increasing its affinity for L-methionine. Can bind NADP (in vitro).By similarity

Pathwayi: S-adenosyl-L-methionine biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes S-adenosyl-L-methionine from L-methionine.By similarity
Proteins known to be involved in this subpathway in this organism are:
  1. S-adenosylmethionine synthase (Mat2a), Methionine adenosyltransferase 2 subunit beta (Mat2b), S-adenosylmethionine synthase isoform type-2 (Mat2a), S-adenosylmethionine synthase (Mat2a), S-adenosylmethionine synthase (Mat2a), S-adenosylmethionine synthase isoform type-1 (Mat1a), Methionine adenosyltransferase 2 subunit beta (Mat2b), S-adenosylmethionine synthase (Mat2a), S-adenosylmethionine synthase (Mat2a), S-adenosylmethionine synthase (Mat2a), S-adenosylmethionine synthase (Mat2a), S-adenosylmethionine synthase (Mat2a)
This subpathway is part of the pathway S-adenosyl-L-methionine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes S-adenosyl-L-methionine from L-methionine, the pathway S-adenosyl-L-methionine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei93NADP; via carbonyl oxygenBy similarity1
Binding sitei97NADPBy similarity1
Binding sitei159NADPBy similarity1
Binding sitei185NADP; via amide nitrogenBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi37 – 40NADPBy similarity4
Nucleotide bindingi60 – 62NADPBy similarity3
Nucleotide bindingi71 – 72NADPBy similarity2

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processOne-carbon metabolism
LigandNADP

Enzyme and pathway databases

ReactomeiR-MMU-156581 Methylation
R-MMU-5689880 Ub-specific processing proteases
UniPathwayiUPA00315; UER00080

Names & Taxonomyi

Protein namesi
Recommended name:
Methionine adenosyltransferase 2 subunit beta
Alternative name(s):
Methionine adenosyltransferase II beta
Short name:
MAT II beta
Gene namesi
Name:Mat2b
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:1913667 Mat2b

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002875211 – 334Methionine adenosyltransferase 2 subunit betaAdd BLAST334

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei309PhosphothreonineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ99LB6
PaxDbiQ99LB6
PeptideAtlasiQ99LB6
PRIDEiQ99LB6

2D gel databases

REPRODUCTION-2DPAGEIPI00649402
Q99LB6

PTM databases

iPTMnetiQ99LB6
PhosphoSitePlusiQ99LB6

Expressioni

Gene expression databases

BgeeiENSMUSG00000042032
CleanExiMM_MAT2B
ExpressionAtlasiQ99LB6 baseline and differential
GenevisibleiQ99LB6 MM

Interactioni

Subunit structurei

Heterotrimer; composed of a catalytic MAT2A homodimer that binds one regulatory MAT2B chain. Heterohexamer; composed of a central, catalytic MAT2A homotetramer flanked on either side by a regulatory MAT2B chain. NADP binding increases the affinity for MAT2A.By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi224352, 1 interactor
IntActiQ99LB6, 3 interactors
MINTiQ99LB6
STRINGi10090.ENSMUSP00000048222

Structurei

3D structure databases

ProteinModelPortaliQ99LB6
SMRiQ99LB6
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni319 – 334Required for interaction with MAT2ABy similarityAdd BLAST16

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG410IQ9F Eukaryota
COG1091 LUCA
GeneTreeiENSGT00390000006721
HOGENOMiHOG000227713
HOVERGENiHBG105851
InParanoidiQ99LB6
KOiK00789
OMAiYHYSNEG
OrthoDBiEOG091G0AS1
PhylomeDBiQ99LB6
TreeFamiTF332849

Family and domain databases

InterProiView protein in InterPro
IPR005913 dTDP_dehydrorham_reduct
IPR036291 NAD(P)-bd_dom_sf
IPR029903 RmlD-like-bd
PANTHERiPTHR10491 PTHR10491, 1 hit
PfamiView protein in Pfam
PF04321 RmlD_sub_bind, 1 hit
SUPFAMiSSF51735 SSF51735, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q99LB6-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MVGREKELSI HFVPGCCQLV EEEVNIPSRR VLITGATGLL GRAVYKEFQQ
60 70 80 90 100
SNWHTVGCGF RRARPKFEQV NLLDSEAVHH LIHDFQPHVI VHCAAERRPD
110 120 130 140 150
VVESQPDAAS QLNVGASGNL AKEAAAIGAF LIYISSDYVF DGTNPPYTEE
160 170 180 190 200
DIPSPLNLYG KTKLDGEKAV LENNLGAAVL RIPVLYGEVE KLEESAVTVM
210 220 230 240 250
FDKVQFSNKS ANMDHWQQRF PTHVKDVASV CRQLAEKRML DPSIKGTFHW
260 270 280 290 300
SGNEQMTKYE MACAIADAFN LPSSHLRPIT DSPVIGAQRP KNAQLDCSKL
310 320 330
ETLGIGQRTP FRTGIKESLW PFLIDKRWRQ TVFH
Length:334
Mass (Da):37,393
Last modified:June 1, 2001 - v1
Checksum:i0B209731D713BD02
GO
Isoform 2 (identifier: Q99LB6-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-21: MVGREKELSIHFVPGCCQLVE → MPEMPEAMEQ

Show »
Length:323
Mass (Da):36,210
Checksum:i95CAF37E602D6949
GO

Sequence cautioni

The sequence BAC29963 differs from that shown. Intron retention.Curated
The sequence CAI25424 differs from that shown. Reason: Erroneous gene model prediction.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti97R → G in BAD06938 (PubMed:16022199).Curated1
Sequence conflicti140F → L in BAD06938 (PubMed:16022199).Curated1
Sequence conflicti170V → A in BAD06938 (PubMed:16022199).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0255411 – 21MVGRE…CQLVE → MPEMPEAMEQ in isoform 2. 1 PublicationAdd BLAST21

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB070267 mRNA Translation: BAD06938.1
AK038303 mRNA Translation: BAC29963.1 Sequence problems.
AK075947 mRNA Translation: BAC36076.1
AK162087 mRNA Translation: BAE36716.1
AL646055 Genomic DNA Translation: CAI25424.1 Sequence problems.
AL646055 Genomic DNA Translation: CAI25422.1
AL646055 Genomic DNA Translation: CAI25423.1
BC003457 mRNA Translation: AAH03457.1
CCDSiCCDS24547.1 [Q99LB6-1]
CCDS56767.1 [Q99LB6-2]
RefSeqiNP_001186203.1, NM_001199274.1 [Q99LB6-2]
NP_598778.1, NM_134017.2 [Q99LB6-1]
UniGeneiMm.293771

Genome annotation databases

EnsembliENSMUST00000040167; ENSMUSP00000048222; ENSMUSG00000042032 [Q99LB6-1]
ENSMUST00000101347; ENSMUSP00000098901; ENSMUSG00000042032 [Q99LB6-2]
GeneIDi108645
KEGGimmu:108645
UCSCiuc007ilp.2 mouse [Q99LB6-1]
uc007ilq.2 mouse [Q99LB6-2]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiMAT2B_MOUSE
AccessioniPrimary (citable) accession number: Q99LB6
Secondary accession number(s): Q5NC89
, Q76LX2, Q8BVX6, Q8BYT9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 15, 2007
Last sequence update: June 1, 2001
Last modified: May 23, 2018
This is version 115 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

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