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Q99LB2

- DHRS4_MOUSE

UniProt

Q99LB2 - DHRS4_MOUSE

Protein

Dehydrogenase/reductase SDR family member 4

Gene

Dhrs4

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 105 (01 Oct 2014)
      Sequence version 3 (03 Oct 2012)
      Previous versions | rss
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    Functioni

    Reduces all-trans-retinal and 9-cis retinal. Can also catalyze the oxidation of all-trans-retinol with NADP as co-factor, but with much lower efficiency. Reduces alkyl phenyl ketones and alpha-dicarbonyl compounds with aromatic rings, such as pyrimidine-4-aldehyde, 3-benzoylpyridine, 4-benzoylpyridine, menadione and 4-hexanoylpyridine. Has no activity towards aliphatic aldehydes and ketones By similarity.By similarity

    Catalytic activityi

    R-CHOH-R' + NADP+ = R-CO-R' + NADPH.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei170 – 1701SubstrateBy similarity
    Active sitei183 – 1831Proton acceptorPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi37 – 6125NADPBy similarityAdd
    BLAST

    GO - Molecular functioni

    1. carbonyl reductase (NADPH) activity Source: UniProtKB-EC
    2. retinal dehydrogenase activity Source: MGI

    GO - Biological processi

    1. retinal metabolic process Source: MGI

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Ligandi

    NADP

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Dehydrogenase/reductase SDR family member 4 (EC:1.1.1.184)
    Alternative name(s):
    NADPH-dependent carbonyl reductase/NADP-retinol dehydrogenase
    Short name:
    CR
    Short name:
    PHCR
    NADPH-dependent retinol dehydrogenase/reductase
    Short name:
    NDRD
    Short name:
    mouNRDR
    Peroxisomal short-chain alcohol dehydrogenase
    Short name:
    PSCD
    Gene namesi
    Name:Dhrs4
    Synonyms:D14Ucla2
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 14

    Organism-specific databases

    MGIiMGI:90169. Dhrs4.

    Subcellular locationi

    Peroxisome By similarity

    GO - Cellular componenti

    1. mitochondrion Source: UniProtKB
    2. peroxisome Source: MGI

    Keywords - Cellular componenti

    Peroxisome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 279279Dehydrogenase/reductase SDR family member 4PRO_0000054648Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei93 – 931N6-acetyllysine; alternate1 Publication
    Modified residuei93 – 931N6-succinyllysine; alternate1 Publication
    Modified residuei106 – 1061N6-acetyllysine1 Publication
    Modified residuei217 – 2171N6-acetyllysine; alternate1 Publication
    Modified residuei217 – 2171N6-succinyllysine; alternate1 Publication
    Modified residuei228 – 2281N6-succinyllysine1 Publication
    Modified residuei235 – 2351N6-succinyllysine1 Publication

    Keywords - PTMi

    Acetylation

    Proteomic databases

    MaxQBiQ99LB2.
    PaxDbiQ99LB2.
    PRIDEiQ99LB2.

    PTM databases

    PhosphoSiteiQ99LB2.

    Expressioni

    Gene expression databases

    ArrayExpressiQ99LB2.
    CleanExiMM_DHRS4.
    GenevestigatoriQ99LB2.

    Interactioni

    Subunit structurei

    Homotetramer.By similarity

    Protein-protein interaction databases

    IntActiQ99LB2. 5 interactions.
    MINTiMINT-1861432.

    Structurei

    3D structure databases

    ProteinModelPortaliQ99LB2.
    SMRiQ99LB2. Positions 26-279.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi277 – 2793Microbody targeting signal

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG1028.
    GeneTreeiENSGT00740000115208.
    HOVERGENiHBG105779.
    InParanoidiQ99LB2.
    KOiK11147.
    OMAiFSQVLWM.
    OrthoDBiEOG73Z2VD.
    TreeFamiTF315405.

    Family and domain databases

    Gene3Di3.40.50.720. 1 hit.
    InterProiIPR002198. DH_sc/Rdtase_SDR.
    IPR029511. DHRS4-like.
    IPR002347. Glc/ribitol_DH.
    IPR016040. NAD(P)-bd_dom.
    IPR020904. Sc_DH/Rdtase_CS.
    [Graphical view]
    PANTHERiPTHR24322:SF198. PTHR24322:SF198. 1 hit.
    PRINTSiPR00081. GDHRDH.
    PR00080. SDRFAMILY.
    PROSITEiPS00061. ADH_SHORT. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q99LB2-1 [UniParc]FASTAAdd to Basket

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    MQKAGRLLGG WTQAWMSVRM ASSGLTRRNP LSNKVALVTA STDGIGFAIA    50
    RRLAEDGAHV VVSSRKQQNV DRAVATLQGE GLSVTGIVCH VGKAEDREKL 100
    ITTALKRHQG IDILVSNAAV NPFFGNLMDV TEEVWDKVLS INVTATAMMI 150
    KAVVPEMEKR GGGSVVIVGS VAGFTRFPSL GPYNVSKTAL LGLTKNFAAE 200
    LAPKNIRVNC LAPGLIKTRF SSVLWEEKAR EDFIKEAMQI RRLGKPEDCA 250
    GIVSFLCSED ASYINGETVV VGGGTPSRL 279
    Length:279
    Mass (Da):29,885
    Last modified:October 3, 2012 - v3
    Checksum:i985668BDE2935072
    GO

    Sequence cautioni

    The sequence AAH03484.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.
    The sequence BAB18776.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti109 – 1091Q → R in AAH03484. (PubMed:15489334)Curated
    Sequence conflicti109 – 1091Q → R in AAH54361. (PubMed:15489334)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB045132 mRNA. Translation: BAB18776.1. Different initiation.
    AC159002 Genomic DNA. No translation available.
    CH466535 Genomic DNA. Translation: EDL36299.1.
    BC003484 mRNA. Translation: AAH03484.1. Different initiation.
    BC054361 mRNA. Translation: AAH54361.1.
    CCDSiCCDS27111.1.
    RefSeqiNP_001033027.2. NM_001037938.2.
    UniGeneiMm.27427.

    Genome annotation databases

    EnsembliENSMUST00000022821; ENSMUSP00000022821; ENSMUSG00000022210.
    GeneIDi28200.
    KEGGimmu:28200.
    UCSCiuc007tym.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB045132 mRNA. Translation: BAB18776.1 . Different initiation.
    AC159002 Genomic DNA. No translation available.
    CH466535 Genomic DNA. Translation: EDL36299.1 .
    BC003484 mRNA. Translation: AAH03484.1 . Different initiation.
    BC054361 mRNA. Translation: AAH54361.1 .
    CCDSi CCDS27111.1.
    RefSeqi NP_001033027.2. NM_001037938.2.
    UniGenei Mm.27427.

    3D structure databases

    ProteinModelPortali Q99LB2.
    SMRi Q99LB2. Positions 26-279.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q99LB2. 5 interactions.
    MINTi MINT-1861432.

    PTM databases

    PhosphoSitei Q99LB2.

    Proteomic databases

    MaxQBi Q99LB2.
    PaxDbi Q99LB2.
    PRIDEi Q99LB2.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000022821 ; ENSMUSP00000022821 ; ENSMUSG00000022210 .
    GeneIDi 28200.
    KEGGi mmu:28200.
    UCSCi uc007tym.2. mouse.

    Organism-specific databases

    CTDi 10901.
    MGIi MGI:90169. Dhrs4.

    Phylogenomic databases

    eggNOGi COG1028.
    GeneTreei ENSGT00740000115208.
    HOVERGENi HBG105779.
    InParanoidi Q99LB2.
    KOi K11147.
    OMAi FSQVLWM.
    OrthoDBi EOG73Z2VD.
    TreeFami TF315405.

    Miscellaneous databases

    NextBioi 306800.
    PROi Q99LB2.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q99LB2.
    CleanExi MM_DHRS4.
    Genevestigatori Q99LB2.

    Family and domain databases

    Gene3Di 3.40.50.720. 1 hit.
    InterProi IPR002198. DH_sc/Rdtase_SDR.
    IPR029511. DHRS4-like.
    IPR002347. Glc/ribitol_DH.
    IPR016040. NAD(P)-bd_dom.
    IPR020904. Sc_DH/Rdtase_CS.
    [Graphical view ]
    PANTHERi PTHR24322:SF198. PTHR24322:SF198. 1 hit.
    PRINTSi PR00081. GDHRDH.
    PR00080. SDRFAMILY.
    PROSITEi PS00061. ADH_SHORT. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "cDNA cloning and characterization of peroxisomal short-chain dehydrogenase / reductase that reduce all-trans retinal to retinol."
      Furukawa A., Ohnishi T., Huang D., Araki N., Ichikawa Y.
      Submitted (JUN-2000) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: C57BL/6.
      Tissue: Liver.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    3. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
      Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Mammary tumor.
    5. "SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways."
      Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y., Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y.
      Mol. Cell 50:919-930(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUCCINYLATION [LARGE SCALE ANALYSIS] AT LYS-93; LYS-217; LYS-228 AND LYS-235, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Liver.
    6. "Label-free quantitative proteomics of the lysine acetylome in mitochondria identifies substrates of SIRT3 in metabolic pathways."
      Rardin M.J., Newman J.C., Held J.M., Cusack M.P., Sorensen D.J., Li B., Schilling B., Mooney S.D., Kahn C.R., Verdin E., Gibson B.W.
      Proc. Natl. Acad. Sci. U.S.A. 110:6601-6606(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-93; LYS-106 AND LYS-217, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Liver.

    Entry informationi

    Entry nameiDHRS4_MOUSE
    AccessioniPrimary (citable) accession number: Q99LB2
    Secondary accession number(s): G3X8V7, Q9EQU4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 10, 2003
    Last sequence update: October 3, 2012
    Last modified: October 1, 2014
    This is version 105 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Inhibited by kaempferol, quercetin, genistein and myristic acid.By similarity

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3