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Q99LB2 (DHRS4_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 104. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Dehydrogenase/reductase SDR family member 4

EC=1.1.1.184
Alternative name(s):
NADPH-dependent carbonyl reductase/NADP-retinol dehydrogenase
Short name=CR
Short name=PHCR
NADPH-dependent retinol dehydrogenase/reductase
Short name=NDRD
Short name=mouNRDR
Peroxisomal short-chain alcohol dehydrogenase
Short name=PSCD
Gene names
Name:Dhrs4
Synonyms:D14Ucla2
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length279 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Reduces all-trans-retinal and 9-cis retinal. Can also catalyze the oxidation of all-trans-retinol with NADP as co-factor, but with much lower efficiency. Reduces alkyl phenyl ketones and alpha-dicarbonyl compounds with aromatic rings, such as pyrimidine-4-aldehyde, 3-benzoylpyridine, 4-benzoylpyridine, menadione and 4-hexanoylpyridine. Has no activity towards aliphatic aldehydes and ketones By similarity.

Catalytic activity

R-CHOH-R' + NADP+ = R-CO-R' + NADPH.

Subunit structure

Homotetramer By similarity.

Subcellular location

Peroxisome By similarity.

Miscellaneous

Inhibited by kaempferol, quercetin, genistein and myristic acid By similarity.

Sequence similarities

Belongs to the short-chain dehydrogenases/reductases (SDR) family.

Sequence caution

The sequence AAH03484.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

The sequence BAB18776.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 279279Dehydrogenase/reductase SDR family member 4
PRO_0000054648

Regions

Nucleotide binding37 – 6125NADP By similarity
Motif277 – 2793Microbody targeting signal

Sites

Active site1831Proton acceptor By similarity
Binding site1701Substrate By similarity

Amino acid modifications

Modified residue931N6-acetyllysine; alternate Ref.6
Modified residue931N6-succinyllysine; alternate Ref.5
Modified residue1061N6-acetyllysine Ref.6
Modified residue2171N6-acetyllysine; alternate Ref.6
Modified residue2171N6-succinyllysine; alternate Ref.5
Modified residue2281N6-succinyllysine Ref.5
Modified residue2351N6-succinyllysine Ref.5

Experimental info

Sequence conflict1091Q → R in AAH03484. Ref.4
Sequence conflict1091Q → R in AAH54361. Ref.4

Sequences

Sequence LengthMass (Da)Tools
Q99LB2 [UniParc].

Last modified October 3, 2012. Version 3.
Checksum: 985668BDE2935072

FASTA27929,885
        10         20         30         40         50         60 
MQKAGRLLGG WTQAWMSVRM ASSGLTRRNP LSNKVALVTA STDGIGFAIA RRLAEDGAHV 

        70         80         90        100        110        120 
VVSSRKQQNV DRAVATLQGE GLSVTGIVCH VGKAEDREKL ITTALKRHQG IDILVSNAAV 

       130        140        150        160        170        180 
NPFFGNLMDV TEEVWDKVLS INVTATAMMI KAVVPEMEKR GGGSVVIVGS VAGFTRFPSL 

       190        200        210        220        230        240 
GPYNVSKTAL LGLTKNFAAE LAPKNIRVNC LAPGLIKTRF SSVLWEEKAR EDFIKEAMQI 

       250        260        270 
RRLGKPEDCA GIVSFLCSED ASYINGETVV VGGGTPSRL 

« Hide

References

« Hide 'large scale' references
[1]"cDNA cloning and characterization of peroxisomal short-chain dehydrogenase / reductase that reduce all-trans retinal to retinol."
Furukawa A., Ohnishi T., Huang D., Araki N., Ichikawa Y.
Submitted (JUN-2000) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: C57BL/6.
Tissue: Liver.
[2]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[3]Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Mammary tumor.
[5]"SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways."
Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y., Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y.
Mol. Cell 50:919-930(2013) [PubMed] [Europe PMC] [Abstract]
Cited for: SUCCINYLATION [LARGE SCALE ANALYSIS] AT LYS-93; LYS-217; LYS-228 AND LYS-235, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Liver.
[6]"Label-free quantitative proteomics of the lysine acetylome in mitochondria identifies substrates of SIRT3 in metabolic pathways."
Rardin M.J., Newman J.C., Held J.M., Cusack M.P., Sorensen D.J., Li B., Schilling B., Mooney S.D., Kahn C.R., Verdin E., Gibson B.W.
Proc. Natl. Acad. Sci. U.S.A. 110:6601-6606(2013) [PubMed] [Europe PMC] [Abstract]
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-93; LYS-106 AND LYS-217, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Liver.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB045132 mRNA. Translation: BAB18776.1. Different initiation.
AC159002 Genomic DNA. No translation available.
CH466535 Genomic DNA. Translation: EDL36299.1.
BC003484 mRNA. Translation: AAH03484.1. Different initiation.
BC054361 mRNA. Translation: AAH54361.1.
CCDSCCDS27111.1.
RefSeqNP_001033027.2. NM_001037938.2.
UniGeneMm.27427.

3D structure databases

ProteinModelPortalQ99LB2.
SMRQ99LB2. Positions 26-279.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActQ99LB2. 5 interactions.
MINTMINT-1861432.

PTM databases

PhosphoSiteQ99LB2.

Proteomic databases

MaxQBQ99LB2.
PaxDbQ99LB2.
PRIDEQ99LB2.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000022821; ENSMUSP00000022821; ENSMUSG00000022210.
GeneID28200.
KEGGmmu:28200.
UCSCuc007tym.2. mouse.

Organism-specific databases

CTD10901.
MGIMGI:90169. Dhrs4.

Phylogenomic databases

eggNOGCOG1028.
GeneTreeENSGT00740000115208.
HOVERGENHBG105779.
InParanoidQ99LB2.
KOK11147.
OMAFSQVLWM.
OrthoDBEOG73Z2VD.
TreeFamTF315405.

Gene expression databases

ArrayExpressQ99LB2.
CleanExMM_DHRS4.
GenevestigatorQ99LB2.

Family and domain databases

Gene3D3.40.50.720. 1 hit.
InterProIPR002198. DH_sc/Rdtase_SDR.
IPR029511. DHRS4-like.
IPR002347. Glc/ribitol_DH.
IPR016040. NAD(P)-bd_dom.
IPR020904. Sc_DH/Rdtase_CS.
[Graphical view]
PANTHERPTHR24322:SF198. PTHR24322:SF198. 1 hit.
PRINTSPR00081. GDHRDH.
PR00080. SDRFAMILY.
PROSITEPS00061. ADH_SHORT. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio306800.
PROQ99LB2.
SOURCESearch...

Entry information

Entry nameDHRS4_MOUSE
AccessionPrimary (citable) accession number: Q99LB2
Secondary accession number(s): G3X8V7, Q9EQU4
Entry history
Integrated into UniProtKB/Swiss-Prot: October 10, 2003
Last sequence update: October 3, 2012
Last modified: July 9, 2014
This is version 104 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot