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Protein

Dehydrogenase/reductase SDR family member 4

Gene

Dhrs4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Reduces all-trans-retinal and 9-cis retinal. Can also catalyze the oxidation of all-trans-retinol with NADP as co-factor, but with much lower efficiency. Reduces alkyl phenyl ketones and alpha-dicarbonyl compounds with aromatic rings, such as pyrimidine-4-aldehyde, 3-benzoylpyridine, 4-benzoylpyridine, menadione and 4-hexanoylpyridine. Has no activity towards aliphatic aldehydes and ketones (By similarity).By similarity

Catalytic activityi

R-CHOH-R' + NADP+ = R-CO-R' + NADPH.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei170 – 1701SubstrateBy similarity
Active sitei183 – 1831Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi37 – 6125NADPBy similarityAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

NADP

Enzyme and pathway databases

ReactomeiREACT_310468. RA biosynthesis pathway.

Names & Taxonomyi

Protein namesi
Recommended name:
Dehydrogenase/reductase SDR family member 4 (EC:1.1.1.184)
Alternative name(s):
NADPH-dependent carbonyl reductase/NADP-retinol dehydrogenase
Short name:
CR
Short name:
PHCR
NADPH-dependent retinol dehydrogenase/reductase
Short name:
NDRD
Short name:
mouNRDR
Peroxisomal short-chain alcohol dehydrogenase
Short name:
PSCD
Gene namesi
Name:Dhrs4
Synonyms:D14Ucla2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 14

Organism-specific databases

MGIiMGI:90169. Dhrs4.

Subcellular locationi

GO - Cellular componenti

  • extracellular exosome Source: MGI
  • intracellular membrane-bounded organelle Source: MGI
  • mitochondrion Source: UniProtKB
  • nuclear membrane Source: MGI
  • nucleus Source: MGI
  • peroxisomal membrane Source: MGI
  • peroxisome Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Peroxisome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 279279Dehydrogenase/reductase SDR family member 4PRO_0000054648Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei93 – 931N6-acetyllysine; alternate1 Publication
Modified residuei93 – 931N6-succinyllysine; alternate1 Publication
Modified residuei106 – 1061N6-acetyllysine1 Publication
Modified residuei217 – 2171N6-acetyllysine; alternate1 Publication
Modified residuei217 – 2171N6-succinyllysine; alternate1 Publication
Modified residuei228 – 2281N6-succinyllysine1 Publication
Modified residuei235 – 2351N6-succinyllysine1 Publication

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiQ99LB2.
PaxDbiQ99LB2.
PRIDEiQ99LB2.

PTM databases

PhosphoSiteiQ99LB2.

Expressioni

Gene expression databases

CleanExiMM_DHRS4.
ExpressionAtlasiQ99LB2. baseline and differential.

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

BioGridi205827. 1 interaction.
IntActiQ99LB2. 5 interactions.
MINTiMINT-1861432.
STRINGi10090.ENSMUSP00000022821.

Structurei

3D structure databases

ProteinModelPortaliQ99LB2.
SMRiQ99LB2. Positions 26-279.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi277 – 2793Microbody targeting signal

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG1028.
GeneTreeiENSGT00760000118868.
HOVERGENiHBG105779.
InParanoidiQ99LB2.
KOiK11147.
OMAiMNGMDDG.
OrthoDBiEOG73Z2VD.
TreeFamiTF315405.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR002198. DH_sc/Rdtase_SDR.
IPR029511. DHRS4-like.
IPR002347. Glc/ribitol_DH.
IPR016040. NAD(P)-bd_dom.
IPR020904. Sc_DH/Rdtase_CS.
[Graphical view]
PANTHERiPTHR24322:SF198. PTHR24322:SF198. 1 hit.
PRINTSiPR00081. GDHRDH.
PR00080. SDRFAMILY.
PROSITEiPS00061. ADH_SHORT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q99LB2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQKAGRLLGG WTQAWMSVRM ASSGLTRRNP LSNKVALVTA STDGIGFAIA
60 70 80 90 100
RRLAEDGAHV VVSSRKQQNV DRAVATLQGE GLSVTGIVCH VGKAEDREKL
110 120 130 140 150
ITTALKRHQG IDILVSNAAV NPFFGNLMDV TEEVWDKVLS INVTATAMMI
160 170 180 190 200
KAVVPEMEKR GGGSVVIVGS VAGFTRFPSL GPYNVSKTAL LGLTKNFAAE
210 220 230 240 250
LAPKNIRVNC LAPGLIKTRF SSVLWEEKAR EDFIKEAMQI RRLGKPEDCA
260 270
GIVSFLCSED ASYINGETVV VGGGTPSRL
Length:279
Mass (Da):29,885
Last modified:October 3, 2012 - v3
Checksum:i985668BDE2935072
GO

Sequence cautioni

The sequence AAH03484.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence BAB18776.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti109 – 1091Q → R in AAH03484 (PubMed:15489334).Curated
Sequence conflicti109 – 1091Q → R in AAH54361 (PubMed:15489334).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB045132 mRNA. Translation: BAB18776.1. Different initiation.
AC159002 Genomic DNA. No translation available.
CH466535 Genomic DNA. Translation: EDL36299.1.
BC003484 mRNA. Translation: AAH03484.1. Different initiation.
BC054361 mRNA. Translation: AAH54361.1.
CCDSiCCDS27111.1.
RefSeqiNP_001033027.2. NM_001037938.2.
UniGeneiMm.27427.

Genome annotation databases

EnsembliENSMUST00000022821; ENSMUSP00000022821; ENSMUSG00000022210.
GeneIDi28200.
KEGGimmu:28200.
UCSCiuc007tym.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB045132 mRNA. Translation: BAB18776.1. Different initiation.
AC159002 Genomic DNA. No translation available.
CH466535 Genomic DNA. Translation: EDL36299.1.
BC003484 mRNA. Translation: AAH03484.1. Different initiation.
BC054361 mRNA. Translation: AAH54361.1.
CCDSiCCDS27111.1.
RefSeqiNP_001033027.2. NM_001037938.2.
UniGeneiMm.27427.

3D structure databases

ProteinModelPortaliQ99LB2.
SMRiQ99LB2. Positions 26-279.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi205827. 1 interaction.
IntActiQ99LB2. 5 interactions.
MINTiMINT-1861432.
STRINGi10090.ENSMUSP00000022821.

PTM databases

PhosphoSiteiQ99LB2.

Proteomic databases

MaxQBiQ99LB2.
PaxDbiQ99LB2.
PRIDEiQ99LB2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000022821; ENSMUSP00000022821; ENSMUSG00000022210.
GeneIDi28200.
KEGGimmu:28200.
UCSCiuc007tym.2. mouse.

Organism-specific databases

CTDi10901.
MGIiMGI:90169. Dhrs4.

Phylogenomic databases

eggNOGiCOG1028.
GeneTreeiENSGT00760000118868.
HOVERGENiHBG105779.
InParanoidiQ99LB2.
KOiK11147.
OMAiMNGMDDG.
OrthoDBiEOG73Z2VD.
TreeFamiTF315405.

Enzyme and pathway databases

ReactomeiREACT_310468. RA biosynthesis pathway.

Miscellaneous databases

NextBioi306800.
PROiQ99LB2.
SOURCEiSearch...

Gene expression databases

CleanExiMM_DHRS4.
ExpressionAtlasiQ99LB2. baseline and differential.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR002198. DH_sc/Rdtase_SDR.
IPR029511. DHRS4-like.
IPR002347. Glc/ribitol_DH.
IPR016040. NAD(P)-bd_dom.
IPR020904. Sc_DH/Rdtase_CS.
[Graphical view]
PANTHERiPTHR24322:SF198. PTHR24322:SF198. 1 hit.
PRINTSiPR00081. GDHRDH.
PR00080. SDRFAMILY.
PROSITEiPS00061. ADH_SHORT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "cDNA cloning and characterization of peroxisomal short-chain dehydrogenase / reductase that reduce all-trans retinal to retinol."
    Furukawa A., Ohnishi T., Huang D., Araki N., Ichikawa Y.
    Submitted (JUN-2000) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: C57BL/6.
    Tissue: Liver.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  3. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Mammary tumor.
  5. "SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways."
    Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y., Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y.
    Mol. Cell 50:919-930(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUCCINYLATION [LARGE SCALE ANALYSIS] AT LYS-93; LYS-217; LYS-228 AND LYS-235, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.
  6. "Label-free quantitative proteomics of the lysine acetylome in mitochondria identifies substrates of SIRT3 in metabolic pathways."
    Rardin M.J., Newman J.C., Held J.M., Cusack M.P., Sorensen D.J., Li B., Schilling B., Mooney S.D., Kahn C.R., Verdin E., Gibson B.W.
    Proc. Natl. Acad. Sci. U.S.A. 110:6601-6606(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-93; LYS-106 AND LYS-217, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.

Entry informationi

Entry nameiDHRS4_MOUSE
AccessioniPrimary (citable) accession number: Q99LB2
Secondary accession number(s): G3X8V7, Q9EQU4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 10, 2003
Last sequence update: October 3, 2012
Last modified: June 24, 2015
This is version 111 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

Inhibited by kaempferol, quercetin, genistein and myristic acid.By similarity

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.