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Q99LB2

- DHRS4_MOUSE

UniProt

Q99LB2 - DHRS4_MOUSE

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Protein
Dehydrogenase/reductase SDR family member 4
Gene
Dhrs4, D14Ucla2
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Reduces all-trans-retinal and 9-cis retinal. Can also catalyze the oxidation of all-trans-retinol with NADP as co-factor, but with much lower efficiency. Reduces alkyl phenyl ketones and alpha-dicarbonyl compounds with aromatic rings, such as pyrimidine-4-aldehyde, 3-benzoylpyridine, 4-benzoylpyridine, menadione and 4-hexanoylpyridine. Has no activity towards aliphatic aldehydes and ketones By similarity.

Catalytic activityi

R-CHOH-R' + NADP+ = R-CO-R' + NADPH.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei170 – 1701Substrate By similarity
Active sitei183 – 1831Proton acceptor By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi37 – 6125NADP By similarity
Add
BLAST

GO - Molecular functioni

  1. carbonyl reductase (NADPH) activity Source: UniProtKB-EC
  2. retinal dehydrogenase activity Source: MGI

GO - Biological processi

  1. retinal metabolic process Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

NADP

Names & Taxonomyi

Protein namesi
Recommended name:
Dehydrogenase/reductase SDR family member 4 (EC:1.1.1.184)
Alternative name(s):
NADPH-dependent carbonyl reductase/NADP-retinol dehydrogenase
Short name:
CR
Short name:
PHCR
NADPH-dependent retinol dehydrogenase/reductase
Short name:
NDRD
Short name:
mouNRDR
Peroxisomal short-chain alcohol dehydrogenase
Short name:
PSCD
Gene namesi
Name:Dhrs4
Synonyms:D14Ucla2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 14

Organism-specific databases

MGIiMGI:90169. Dhrs4.

Subcellular locationi

Peroxisome By similarity

GO - Cellular componenti

  1. mitochondrion Source: UniProtKB
  2. peroxisome Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Peroxisome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 279279Dehydrogenase/reductase SDR family member 4
PRO_0000054648Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei93 – 931N6-acetyllysine; alternate1 Publication
Modified residuei93 – 931N6-succinyllysine; alternate1 Publication
Modified residuei106 – 1061N6-acetyllysine1 Publication
Modified residuei217 – 2171N6-acetyllysine; alternate1 Publication
Modified residuei217 – 2171N6-succinyllysine; alternate1 Publication
Modified residuei228 – 2281N6-succinyllysine1 Publication
Modified residuei235 – 2351N6-succinyllysine1 Publication

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiQ99LB2.
PaxDbiQ99LB2.
PRIDEiQ99LB2.

PTM databases

PhosphoSiteiQ99LB2.

Expressioni

Gene expression databases

ArrayExpressiQ99LB2.
CleanExiMM_DHRS4.
GenevestigatoriQ99LB2.

Interactioni

Subunit structurei

Homotetramer By similarity.

Protein-protein interaction databases

IntActiQ99LB2. 5 interactions.
MINTiMINT-1861432.

Structurei

3D structure databases

ProteinModelPortaliQ99LB2.
SMRiQ99LB2. Positions 26-279.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi277 – 2793Microbody targeting signal

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG1028.
GeneTreeiENSGT00740000115208.
HOVERGENiHBG105779.
InParanoidiQ99LB2.
KOiK11147.
OMAiFSQVLWM.
OrthoDBiEOG73Z2VD.
TreeFamiTF315405.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR002198. DH_sc/Rdtase_SDR.
IPR029511. DHRS4-like.
IPR002347. Glc/ribitol_DH.
IPR016040. NAD(P)-bd_dom.
IPR020904. Sc_DH/Rdtase_CS.
[Graphical view]
PANTHERiPTHR24322:SF198. PTHR24322:SF198. 1 hit.
PRINTSiPR00081. GDHRDH.
PR00080. SDRFAMILY.
PROSITEiPS00061. ADH_SHORT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q99LB2-1 [UniParc]FASTAAdd to Basket

« Hide

MQKAGRLLGG WTQAWMSVRM ASSGLTRRNP LSNKVALVTA STDGIGFAIA    50
RRLAEDGAHV VVSSRKQQNV DRAVATLQGE GLSVTGIVCH VGKAEDREKL 100
ITTALKRHQG IDILVSNAAV NPFFGNLMDV TEEVWDKVLS INVTATAMMI 150
KAVVPEMEKR GGGSVVIVGS VAGFTRFPSL GPYNVSKTAL LGLTKNFAAE 200
LAPKNIRVNC LAPGLIKTRF SSVLWEEKAR EDFIKEAMQI RRLGKPEDCA 250
GIVSFLCSED ASYINGETVV VGGGTPSRL 279
Length:279
Mass (Da):29,885
Last modified:October 3, 2012 - v3
Checksum:i985668BDE2935072
GO

Sequence cautioni

The sequence AAH03484.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.
The sequence BAB18776.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti109 – 1091Q → R in AAH03484. 1 Publication
Sequence conflicti109 – 1091Q → R in AAH54361. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB045132 mRNA. Translation: BAB18776.1. Different initiation.
AC159002 Genomic DNA. No translation available.
CH466535 Genomic DNA. Translation: EDL36299.1.
BC003484 mRNA. Translation: AAH03484.1. Different initiation.
BC054361 mRNA. Translation: AAH54361.1.
CCDSiCCDS27111.1.
RefSeqiNP_001033027.2. NM_001037938.2.
UniGeneiMm.27427.

Genome annotation databases

EnsembliENSMUST00000022821; ENSMUSP00000022821; ENSMUSG00000022210.
GeneIDi28200.
KEGGimmu:28200.
UCSCiuc007tym.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB045132 mRNA. Translation: BAB18776.1 . Different initiation.
AC159002 Genomic DNA. No translation available.
CH466535 Genomic DNA. Translation: EDL36299.1 .
BC003484 mRNA. Translation: AAH03484.1 . Different initiation.
BC054361 mRNA. Translation: AAH54361.1 .
CCDSi CCDS27111.1.
RefSeqi NP_001033027.2. NM_001037938.2.
UniGenei Mm.27427.

3D structure databases

ProteinModelPortali Q99LB2.
SMRi Q99LB2. Positions 26-279.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi Q99LB2. 5 interactions.
MINTi MINT-1861432.

PTM databases

PhosphoSitei Q99LB2.

Proteomic databases

MaxQBi Q99LB2.
PaxDbi Q99LB2.
PRIDEi Q99LB2.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000022821 ; ENSMUSP00000022821 ; ENSMUSG00000022210 .
GeneIDi 28200.
KEGGi mmu:28200.
UCSCi uc007tym.2. mouse.

Organism-specific databases

CTDi 10901.
MGIi MGI:90169. Dhrs4.

Phylogenomic databases

eggNOGi COG1028.
GeneTreei ENSGT00740000115208.
HOVERGENi HBG105779.
InParanoidi Q99LB2.
KOi K11147.
OMAi FSQVLWM.
OrthoDBi EOG73Z2VD.
TreeFami TF315405.

Miscellaneous databases

NextBioi 306800.
PROi Q99LB2.
SOURCEi Search...

Gene expression databases

ArrayExpressi Q99LB2.
CleanExi MM_DHRS4.
Genevestigatori Q99LB2.

Family and domain databases

Gene3Di 3.40.50.720. 1 hit.
InterProi IPR002198. DH_sc/Rdtase_SDR.
IPR029511. DHRS4-like.
IPR002347. Glc/ribitol_DH.
IPR016040. NAD(P)-bd_dom.
IPR020904. Sc_DH/Rdtase_CS.
[Graphical view ]
PANTHERi PTHR24322:SF198. PTHR24322:SF198. 1 hit.
PRINTSi PR00081. GDHRDH.
PR00080. SDRFAMILY.
PROSITEi PS00061. ADH_SHORT. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "cDNA cloning and characterization of peroxisomal short-chain dehydrogenase / reductase that reduce all-trans retinal to retinol."
    Furukawa A., Ohnishi T., Huang D., Araki N., Ichikawa Y.
    Submitted (JUN-2000) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: C57BL/6.
    Tissue: Liver.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  3. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Mammary tumor.
  5. "SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways."
    Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y., Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y.
    Mol. Cell 50:919-930(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUCCINYLATION [LARGE SCALE ANALYSIS] AT LYS-93; LYS-217; LYS-228 AND LYS-235, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.
  6. "Label-free quantitative proteomics of the lysine acetylome in mitochondria identifies substrates of SIRT3 in metabolic pathways."
    Rardin M.J., Newman J.C., Held J.M., Cusack M.P., Sorensen D.J., Li B., Schilling B., Mooney S.D., Kahn C.R., Verdin E., Gibson B.W.
    Proc. Natl. Acad. Sci. U.S.A. 110:6601-6606(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-93; LYS-106 AND LYS-217, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.

Entry informationi

Entry nameiDHRS4_MOUSE
AccessioniPrimary (citable) accession number: Q99LB2
Secondary accession number(s): G3X8V7, Q9EQU4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 10, 2003
Last sequence update: October 3, 2012
Last modified: July 9, 2014
This is version 104 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

Inhibited by kaempferol, quercetin, genistein and myristic acid By similarity.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi