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Protein

Dehydrogenase/reductase SDR family member 4

Gene

Dhrs4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Reduces all-trans-retinal and 9-cis retinal. Can also catalyze the oxidation of all-trans-retinol with NADP as co-factor, but with much lower efficiency. Reduces alkyl phenyl ketones and alpha-dicarbonyl compounds with aromatic rings, such as pyrimidine-4-aldehyde, 3-benzoylpyridine, 4-benzoylpyridine, menadione and 4-hexanoylpyridine. Has no activity towards aliphatic aldehydes and ketones (By similarity).By similarity

Catalytic activityi

R-CHOH-R' + NADP+ = R-CO-R' + NADPH.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei170SubstrateBy similarity1
Active sitei183Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi37 – 61NADPBy similarityAdd BLAST25

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

NADP

Enzyme and pathway databases

ReactomeiR-MMU-5365859. RA biosynthesis pathway.

Names & Taxonomyi

Protein namesi
Recommended name:
Dehydrogenase/reductase SDR family member 4 (EC:1.1.1.184)
Alternative name(s):
NADPH-dependent carbonyl reductase/NADP-retinol dehydrogenase
Short name:
CR
Short name:
PHCR
NADPH-dependent retinol dehydrogenase/reductase
Short name:
NDRD
Short name:
mouNRDR
Peroxisomal short-chain alcohol dehydrogenase
Short name:
PSCD
Gene namesi
Name:Dhrs4
Synonyms:D14Ucla2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 14

Organism-specific databases

MGIiMGI:90169. Dhrs4.

Subcellular locationi

GO - Cellular componenti

  • extracellular exosome Source: MGI
  • intracellular membrane-bounded organelle Source: MGI
  • mitochondrion Source: UniProtKB
  • nuclear membrane Source: MGI
  • peroxisomal membrane Source: MGI
  • peroxisome Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Peroxisome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000546481 – 279Dehydrogenase/reductase SDR family member 4Add BLAST279

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei93N6-acetyllysine; alternateCombined sources1
Modified residuei93N6-succinyllysine; alternateCombined sources1
Modified residuei106N6-acetyllysineCombined sources1
Modified residuei217N6-acetyllysine; alternateCombined sources1
Modified residuei217N6-succinyllysine; alternateCombined sources1
Modified residuei221PhosphoserineCombined sources1
Modified residuei228N6-succinyllysineCombined sources1
Modified residuei235N6-succinyllysineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ99LB2.
MaxQBiQ99LB2.
PaxDbiQ99LB2.
PRIDEiQ99LB2.

PTM databases

iPTMnetiQ99LB2.
PhosphoSitePlusiQ99LB2.
SwissPalmiQ99LB2.

Expressioni

Gene expression databases

BgeeiENSMUSG00000022210.
CleanExiMM_DHRS4.
GenevisibleiQ99LB2. MM.

Interactioni

Subunit structurei

Homotetramer.By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi205827. 1 interactor.
IntActiQ99LB2. 5 interactors.
MINTiMINT-1861432.
STRINGi10090.ENSMUSP00000022821.

Structurei

3D structure databases

ProteinModelPortaliQ99LB2.
SMRiQ99LB2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi277 – 279Microbody targeting signal3

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0725. Eukaryota.
COG1028. LUCA.
GeneTreeiENSGT00760000118868.
HOVERGENiHBG105779.
InParanoidiQ99LB2.
KOiK11147.
OMAiLWMDKAR.
OrthoDBiEOG091G0G3R.
TreeFamiTF315405.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR029511. DHRS4-like.
IPR016040. NAD(P)-bd_dom.
IPR020904. Sc_DH/Rdtase_CS.
IPR002347. SDR_fam.
[Graphical view]
PANTHERiPTHR24322. PTHR24322. 2 hits.
PTHR24322:SF198. PTHR24322:SF198. 2 hits.
PRINTSiPR00081. GDHRDH.
PR00080. SDRFAMILY.
SUPFAMiSSF51735. SSF51735. 1 hit.
PROSITEiPS00061. ADH_SHORT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q99LB2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQKAGRLLGG WTQAWMSVRM ASSGLTRRNP LSNKVALVTA STDGIGFAIA
60 70 80 90 100
RRLAEDGAHV VVSSRKQQNV DRAVATLQGE GLSVTGIVCH VGKAEDREKL
110 120 130 140 150
ITTALKRHQG IDILVSNAAV NPFFGNLMDV TEEVWDKVLS INVTATAMMI
160 170 180 190 200
KAVVPEMEKR GGGSVVIVGS VAGFTRFPSL GPYNVSKTAL LGLTKNFAAE
210 220 230 240 250
LAPKNIRVNC LAPGLIKTRF SSVLWEEKAR EDFIKEAMQI RRLGKPEDCA
260 270
GIVSFLCSED ASYINGETVV VGGGTPSRL
Length:279
Mass (Da):29,885
Last modified:October 3, 2012 - v3
Checksum:i985668BDE2935072
GO

Sequence cautioni

The sequence AAH03484 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence BAB18776 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti109Q → R in AAH03484 (PubMed:15489334).Curated1
Sequence conflicti109Q → R in AAH54361 (PubMed:15489334).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB045132 mRNA. Translation: BAB18776.1. Different initiation.
AC159002 Genomic DNA. No translation available.
CH466535 Genomic DNA. Translation: EDL36299.1.
BC003484 mRNA. Translation: AAH03484.1. Different initiation.
BC054361 mRNA. Translation: AAH54361.1.
CCDSiCCDS27111.1.
RefSeqiNP_001033027.2. NM_001037938.2.
UniGeneiMm.27427.

Genome annotation databases

EnsembliENSMUST00000022821; ENSMUSP00000022821; ENSMUSG00000022210.
GeneIDi28200.
KEGGimmu:28200.
UCSCiuc007tym.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB045132 mRNA. Translation: BAB18776.1. Different initiation.
AC159002 Genomic DNA. No translation available.
CH466535 Genomic DNA. Translation: EDL36299.1.
BC003484 mRNA. Translation: AAH03484.1. Different initiation.
BC054361 mRNA. Translation: AAH54361.1.
CCDSiCCDS27111.1.
RefSeqiNP_001033027.2. NM_001037938.2.
UniGeneiMm.27427.

3D structure databases

ProteinModelPortaliQ99LB2.
SMRiQ99LB2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi205827. 1 interactor.
IntActiQ99LB2. 5 interactors.
MINTiMINT-1861432.
STRINGi10090.ENSMUSP00000022821.

PTM databases

iPTMnetiQ99LB2.
PhosphoSitePlusiQ99LB2.
SwissPalmiQ99LB2.

Proteomic databases

EPDiQ99LB2.
MaxQBiQ99LB2.
PaxDbiQ99LB2.
PRIDEiQ99LB2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000022821; ENSMUSP00000022821; ENSMUSG00000022210.
GeneIDi28200.
KEGGimmu:28200.
UCSCiuc007tym.2. mouse.

Organism-specific databases

CTDi10901.
MGIiMGI:90169. Dhrs4.

Phylogenomic databases

eggNOGiKOG0725. Eukaryota.
COG1028. LUCA.
GeneTreeiENSGT00760000118868.
HOVERGENiHBG105779.
InParanoidiQ99LB2.
KOiK11147.
OMAiLWMDKAR.
OrthoDBiEOG091G0G3R.
TreeFamiTF315405.

Enzyme and pathway databases

ReactomeiR-MMU-5365859. RA biosynthesis pathway.

Miscellaneous databases

PROiQ99LB2.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000022210.
CleanExiMM_DHRS4.
GenevisibleiQ99LB2. MM.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR029511. DHRS4-like.
IPR016040. NAD(P)-bd_dom.
IPR020904. Sc_DH/Rdtase_CS.
IPR002347. SDR_fam.
[Graphical view]
PANTHERiPTHR24322. PTHR24322. 2 hits.
PTHR24322:SF198. PTHR24322:SF198. 2 hits.
PRINTSiPR00081. GDHRDH.
PR00080. SDRFAMILY.
SUPFAMiSSF51735. SSF51735. 1 hit.
PROSITEiPS00061. ADH_SHORT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDHRS4_MOUSE
AccessioniPrimary (citable) accession number: Q99LB2
Secondary accession number(s): G3X8V7, Q9EQU4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 10, 2003
Last sequence update: October 3, 2012
Last modified: November 2, 2016
This is version 124 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

Inhibited by kaempferol, quercetin, genistein and myristic acid.By similarity

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.