ID   Q99L96_MOUSE            Unreviewed;       245 AA.
AC   Q99L96;
DT   01-JUN-2001, integrated into UniProtKB/TrEMBL.
DT   01-JUN-2001, sequence version 1.
DT   27-MAR-2024, entry version 145.
DE   RecName: Full=Tetraspanin {ECO:0000256|RuleBase:RU361218};
GN   Name=Tspan6 {ECO:0000313|EMBL:AAH03733.1,
GN   ECO:0000313|Ensembl:ENSMUSP00000084838.6,
GN   ECO:0000313|MGI:MGI:1926264};
GN   Synonyms=Tm4sf6 {ECO:0000313|MGI:MGI:1926264};
OS   Mus musculus (Mouse).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Mus; Mus.
OX   NCBI_TaxID=10090 {ECO:0000313|EMBL:AAH03733.1};
RN   [1] {ECO:0000313|EMBL:BAE27349.1}
RP   NUCLEOTIDE SEQUENCE.
RC   STRAIN=C57BL/6J {ECO:0000313|EMBL:BAE27349.1};
RC   TISSUE=Kidney {ECO:0000313|EMBL:BAE27349.1}, Placenta
RC   {ECO:0000313|EMBL:BAE39476.1}, and Sympathetic ganglion
RC   {ECO:0000313|EMBL:BAE28764.1};
RX   PubMed=10349636; DOI=10.1016/S0076-6879(99)03004-9;
RA   Carninci P., Hayashizaki Y.;
RT   "High-efficiency full-length cDNA cloning.";
RL   Methods Enzymol. 303:19-44(1999).
RN   [2] {ECO:0000313|EMBL:BAE27349.1}
RP   NUCLEOTIDE SEQUENCE.
RC   STRAIN=C57BL/6J {ECO:0000313|EMBL:BAE27349.1};
RC   TISSUE=Kidney {ECO:0000313|EMBL:BAE27349.1}, Placenta
RC   {ECO:0000313|EMBL:BAE39476.1}, and Sympathetic ganglion
RC   {ECO:0000313|EMBL:BAE28764.1};
RX   PubMed=11042159; DOI=10.1101/gr.145100;
RA   Carninci P., Shibata Y., Hayatsu N., Sugahara Y., Shibata K., Itoh M.,
RA   Konno H., Okazaki Y., Muramatsu M., Hayashizaki Y.;
RT   "Normalization and subtraction of cap-trapper-selected cDNAs to prepare
RT   full-length cDNA libraries for rapid discovery of new genes.";
RL   Genome Res. 10:1617-1630(2000).
RN   [3] {ECO:0000313|EMBL:BAE27349.1}
RP   NUCLEOTIDE SEQUENCE.
RC   STRAIN=C57BL/6J {ECO:0000313|EMBL:BAE27349.1};
RC   TISSUE=Kidney {ECO:0000313|EMBL:BAE27349.1}, Placenta
RC   {ECO:0000313|EMBL:BAE39476.1}, and Sympathetic ganglion
RC   {ECO:0000313|EMBL:BAE28764.1};
RX   PubMed=11076861; DOI=10.1101/gr.152600;
RA   Shibata K., Itoh M., Aizawa K., Nagaoka S., Sasaki N., Carninci P.,
RA   Konno H., Akiyama J., Nishi K., Kitsunai T., Tashiro H., Itoh M., Sumi N.,
RA   Ishii Y., Nakamura S., Hazama M., Nishine T., Harada A., Yamamoto R.,
RA   Matsumoto H., Sakaguchi S., Ikegami T., Kashiwagi K., Fujiwake S.,
RA   Inoue K., Togawa Y., Izawa M., Ohara E., Watahiki M., Yoneda Y.,
RA   Ishikawa T., Ozawa K., Tanaka T., Matsuura S., Kawai J., Okazaki Y.,
RA   Muramatsu M., Inoue Y., Kira A., Hayashizaki Y.;
RT   "RIKEN integrated sequence analysis (RISA) system--384-format sequencing
RT   pipeline with 384 multicapillary sequencer.";
RL   Genome Res. 10:1757-1771(2000).
RN   [4] {ECO:0000313|EMBL:BAE27349.1}
RP   NUCLEOTIDE SEQUENCE.
RC   STRAIN=C57BL/6J {ECO:0000313|EMBL:BAE27349.1};
RC   TISSUE=Kidney {ECO:0000313|EMBL:BAE27349.1}, Placenta
RC   {ECO:0000313|EMBL:BAE39476.1}, and Sympathetic ganglion
RC   {ECO:0000313|EMBL:BAE28764.1};
RX   PubMed=11217851; DOI=10.1038/35055500;
RG   The RIKEN Genome Exploration Research Group Phase II Team and the FANTOM Consortium;
RT   "Functional annotation of a full-length mouse cDNA collection.";
RL   Nature 409:685-690(2001).
RN   [5] {ECO:0000313|EMBL:BAE27349.1}
RP   NUCLEOTIDE SEQUENCE.
RC   STRAIN=C57BL/6J {ECO:0000313|EMBL:BAE27349.1};
RC   TISSUE=Kidney {ECO:0000313|EMBL:BAE27349.1}, Placenta
RC   {ECO:0000313|EMBL:BAE39476.1}, and Sympathetic ganglion
RC   {ECO:0000313|EMBL:BAE28764.1};
RX   PubMed=12466851; DOI=10.1038/nature01266;
RG   The FANTOM Consortium and the RIKEN Genome Exploration Research Group Phase I and II Team;
RT   "Analysis of the mouse transcriptome based on functional annotation of
RT   60,770 full-length cDNAs.";
RL   Nature 420:563-573(2002).
RN   [6] {ECO:0000313|EMBL:AAH03733.1}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=Czech II {ECO:0000313|EMBL:AAH03733.1}, and FVB/N
RC   {ECO:0000313|EMBL:AAI10315.1};
RC   TISSUE=Mammary tumor metastatized to lung. Tumor arose spontaneously
RC   {ECO:0000313|EMBL:AAH03733.1}, and Mammary tumor. C3
RC   {ECO:0000313|EMBL:AAI10315.1};
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RA   Gerhard D.S., Wagner L., Feingold E.A., Shenmen C.M., Grouse L.H.,
RA   Schuler G., Klein S.L., Old S., Rasooly R., Good P., Guyer M., Peck A.M.,
RA   Derge J.G., Lipman D., Collins F.S., Jang W., Sherry S., Feolo M.,
RA   Misquitta L., Lee E., Rotmistrovsky K., Greenhut S.F., Schaefer C.F.,
RA   Buetow K., Bonner T.I., Haussler D., Kent J., Kiekhaus M., Furey T.,
RA   Brent M., Prange C., Schreiber K., Shapiro N., Bhat N.K., Hopkins R.F.,
RA   Hsie F., Driscoll T., Soares M.B., Casavant T.L., Scheetz T.E.,
RA   Brown-stein M.J., Usdin T.B., Toshiyuki S., Carninci P., Piao Y.,
RA   Dudekula D.B., Ko M.S., Kawakami K., Suzuki Y., Sugano S., Gruber C.E.,
RA   Smith M.R., Simmons B., Moore T., Waterman R., Johnson S.L., Ruan Y.,
RA   Wei C.L., Mathavan S., Gunaratne P.H., Wu J., Garcia A.M., Hulyk S.W.,
RA   Fuh E., Yuan Y., Sneed A., Kowis C., Hodgson A., Muzny D.M., McPherson J.,
RA   Gibbs R.A., Fahey J., Helton E., Ketteman M., Madan A., Rodrigues S.,
RA   Sanchez A., Whiting M., Madari A., Young A.C., Wetherby K.D., Granite S.J.,
RA   Kwong P.N., Brinkley C.P., Pearson R.L., Bouffard G.G., Blakesly R.W.,
RA   Green E.D., Dickson M.C., Rodriguez A.C., Grimwood J., Schmutz J.,
RA   Myers R.M., Butterfield Y.S., Griffith M., Griffith O.L., Krzywinski M.I.,
RA   Liao N., Morin R., Morrin R., Palmquist D., Petrescu A.S., Skalska U.,
RA   Smailus D.E., Stott J.M., Schnerch A., Schein J.E., Jones S.J., Holt R.A.,
RA   Baross A., Marra M.A., Clifton S., Makowski K.A., Bosak S., Malek J.;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [7] {ECO:0000313|EMBL:BAE27349.1}
RP   NUCLEOTIDE SEQUENCE.
RC   STRAIN=C57BL/6J {ECO:0000313|EMBL:BAE27349.1};
RC   TISSUE=Kidney {ECO:0000313|EMBL:BAE27349.1}, Placenta
RC   {ECO:0000313|EMBL:BAE39476.1}, and Sympathetic ganglion
RC   {ECO:0000313|EMBL:BAE28764.1};
RA   Arakawa T., Carninci P., Fukuda S., Hashizume W., Hayashida K., Hori F.,
RA   Iida J., Imamura K., Imotani K., Itoh M., Kanagawa S., Kawai J., Kojima M.,
RA   Konno H., Murata M., Nakamura M., Ninomiya N., Nishiyori H., Nomura K.,
RA   Ohno M., Sakazume N., Sano H., Sasaki D., Shibata K., Shiraki T.,
RA   Tagami M., Tagami Y., Waki K., Watahiki A., Muramatsu M., Hayashizaki Y.;
RL   Submitted (MAR-2004) to the EMBL/GenBank/DDBJ databases.
RN   [8] {ECO:0000313|EMBL:BAE27349.1}
RP   NUCLEOTIDE SEQUENCE.
RC   STRAIN=C57BL/6J {ECO:0000313|EMBL:BAE27349.1};
RC   TISSUE=Kidney {ECO:0000313|EMBL:BAE27349.1}, Placenta
RC   {ECO:0000313|EMBL:BAE39476.1}, and Sympathetic ganglion
RC   {ECO:0000313|EMBL:BAE28764.1};
RG   The FANTOM Consortium;
RG   Riken Genome Exploration Research Group and Genome Science Group (Genome Network Project Core Group);
RT   "The Transcriptional Landscape of the Mammalian Genome.";
RL   Science 309:1559-1563(2005).
RN   [9] {ECO:0000313|EMBL:BAE27349.1}
RP   NUCLEOTIDE SEQUENCE.
RC   STRAIN=C57BL/6J {ECO:0000313|EMBL:BAE27349.1};
RC   TISSUE=Kidney {ECO:0000313|EMBL:BAE27349.1}, Placenta
RC   {ECO:0000313|EMBL:BAE39476.1}, and Sympathetic ganglion
RC   {ECO:0000313|EMBL:BAE28764.1};
RX   PubMed=16141073; DOI=10.1126/science.1112009;
RG   RIKEN Genome Exploration Research Group and Genome Science Group (Genome Network Project Core Group) and the FANTOM Consortium;
RT   "Antisense Transcription in the Mammalian Transcriptome.";
RL   Science 309:1564-1566(2005).
RN   [10] {ECO:0000313|Ensembl:ENSMUSP00000084838.6, ECO:0000313|Proteomes:UP000000589}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=C57BL/6J {ECO:0000313|Ensembl:ENSMUSP00000084838.6,
RC   ECO:0000313|Proteomes:UP000000589};
RX   PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA   Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA   Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA   Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA   Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA   Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA   Eichler E.E., Ponting C.P.;
RT   "Lineage-specific biology revealed by a finished genome assembly of the
RT   mouse.";
RL   PLoS Biol. 7:E1000112-E1000112(2009).
RN   [11] {ECO:0007829|PubMed:21183079}
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX   PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA   Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA   Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT   "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL   Cell 143:1174-1189(2010).
RN   [12] {ECO:0000313|Ensembl:ENSMUSP00000084838.6}
RP   IDENTIFICATION.
RC   STRAIN=C57BL/6J {ECO:0000313|Ensembl:ENSMUSP00000084838.6};
RG   Ensembl;
RL   Submitted (NOV-2023) to UniProtKB.
CC   -!- SUBCELLULAR LOCATION: Membrane {ECO:0000256|ARBA:ARBA00004141,
CC       ECO:0000256|RuleBase:RU361218}; Multi-pass membrane protein
CC       {ECO:0000256|ARBA:ARBA00004141, ECO:0000256|RuleBase:RU361218}.
CC   -!- SIMILARITY: Belongs to the tetraspanin (TM4SF) family.
CC       {ECO:0000256|ARBA:ARBA00006840, ECO:0000256|RuleBase:RU361218}.
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DR   EMBL; BC003733; AAH03733.1; -; mRNA.
DR   EMBL; BC110314; AAI10315.1; -; mRNA.
DR   EMBL; AK146673; BAE27349.1; -; mRNA.
DR   EMBL; AK149204; BAE28764.1; -; mRNA.
DR   EMBL; AK167384; BAE39476.1; -; mRNA.
DR   RefSeq; NP_062630.2; NM_019656.3.
DR   ProteomicsDB; 340628; -.
DR   Antibodypedia; 478; 265 antibodies from 27 providers.
DR   DNASU; 56496; -.
DR   Ensembl; ENSMUST00000087557.12; ENSMUSP00000084838.6; ENSMUSG00000067377.13.
DR   GeneID; 56496; -.
DR   KEGG; mmu:56496; -.
DR   UCSC; uc009ufa.1; mouse.
DR   AGR; MGI:1926264; -.
DR   CTD; 7105; -.
DR   MGI; MGI:1926264; Tspan6.
DR   VEuPathDB; HostDB:ENSMUSG00000067377; -.
DR   GeneTree; ENSGT00940000159092; -.
DR   OMA; FLFRHEI; -.
DR   OrthoDB; 2913577at2759; -.
DR   TreeFam; TF352891; -.
DR   BioGRID-ORCS; 56496; 2 hits in 77 CRISPR screens.
DR   ChiTaRS; Tspan6; mouse.
DR   Proteomes; UP000000589; Chromosome X.
DR   Bgee; ENSMUSG00000067377; Expressed in saccule of membranous labyrinth and 254 other cell types or tissues.
DR   GO; GO:0016020; C:membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0043124; P:negative regulation of canonical NF-kappaB signal transduction; IEA:Ensembl.
DR   GO; GO:0039532; P:negative regulation of cytoplasmic pattern recognition receptor signaling pathway; IEA:Ensembl.
DR   CDD; cd03161; TM4SF2_6_like_LEL; 1.
DR   Gene3D; 1.10.1450.10; Tetraspanin; 1.
DR   InterPro; IPR018499; Tetraspanin/Peripherin.
DR   InterPro; IPR000301; Tetraspanin_animals.
DR   InterPro; IPR018503; Tetraspanin_CS.
DR   InterPro; IPR008952; Tetraspanin_EC2_sf.
DR   InterPro; IPR048232; TSN6/7_LEL.
DR   PANTHER; PTHR19282; TETRASPANIN; 1.
DR   PANTHER; PTHR19282:SF169; TETRASPANIN-6; 1.
DR   Pfam; PF00335; Tetraspanin; 1.
DR   PIRSF; PIRSF002419; Tetraspanin; 1.
DR   PRINTS; PR00259; TMFOUR.
DR   SUPFAM; SSF48652; Tetraspanin; 1.
DR   PROSITE; PS00421; TM4_1; 1.
PE   1: Evidence at protein level;
KW   Glycoprotein {ECO:0000256|ARBA:ARBA00023180};
KW   Membrane {ECO:0000256|ARBA:ARBA00023136, ECO:0000256|RuleBase:RU361218};
KW   Proteomics identification {ECO:0007829|EPD:Q99L96,
KW   ECO:0007829|MaxQB:Q99L96};
KW   Reference proteome {ECO:0000313|Proteomes:UP000000589};
KW   Transmembrane {ECO:0000256|RuleBase:RU361218};
KW   Transmembrane helix {ECO:0000256|RuleBase:RU361218}.
FT   TRANSMEM        21..42
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|RuleBase:RU361218"
FT   TRANSMEM        62..80
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|RuleBase:RU361218"
FT   TRANSMEM        92..114
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|RuleBase:RU361218"
FT   TRANSMEM        211..233
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|RuleBase:RU361218"
SQ   SEQUENCE   245 AA;  27303 MW;  895142C6381037A4 CRC64;
     MASPSRRLQT KPVITCLKSV LLIYTFIFWI TGVILLAVGI WGKVSLENYF SLLNEKATNV
     PFVLIGTGTV IILLGTFGCF ATCRASAWML KLYAMFLTLI FLVELVAAIV GFVFRHEIKN
     SFKSNYENAL KEYNSTGDYR SEAVDKIQST LHCCGVTNYG DWKGTNYYSE TGFPKSCCKL
     EGCYPQRDAD KVNEEGCFIK VMTTIESEMG VVAGISFGVA CFQLIGIFLA YCLSRAITNN
     QYEIV
//