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Protein

PAXIP1-associated glutamate-rich protein 1A

Gene

Pagr1a

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Its association with the histone methyltransferase MLL2/MLL3 complex is suggesting a role in epigenetic transcriptional activation. However, in association with PAXIP1/PTIP is proposed to function at least in part independently of the MLL2/MLL3 complex. Proposed to be recruited by PAXIP1 to sites of DNA damage where the PAGR1:PAXIP1 complex is required for cell survival in response to DNA damage independently of the MLL2/MLL3 complex (PubMed:19124460). However, its function in DNA damage has been questioned (PubMed:26744420). During immunoglobulin class switching in activated B-cells is involved in transcription regulation of downstream switch regions at the immunoglobulin heavy-chain (Igh) locus independently of the MLL2/MLL3 complex (PubMed:26744420). Involved in both estrogen receptor-regulated gene transcription and estrogen-stimulated G1/S cell-cycle transition (By similarity). Acts as transcriptional cofactor for nuclear hormone receptors. Inhibits the induction properties of several steroid receptors such as NR3C1, AR and PPARG; the mechanism of inhibition appears to be gene-dependent (By similarity). May be involved in the regulation of the BMP pathway in extraembryonic development (PubMed:24633704).By similarityCurated2 Publications

GO - Molecular functioni

GO - Biological processi

  • chorion development Source: MGI
  • DNA recombination Source: UniProtKB-KW
  • DNA repair Source: UniProtKB-KW
  • histone H3-K27 demethylation Source: MGI
  • positive regulation of cell cycle G1/S phase transition Source: MGI
  • positive regulation of intracellular estrogen receptor signaling pathway Source: MGI
  • positive regulation of isotype switching to IgG isotypes Source: UniProtKB
  • positive regulation of response to DNA damage stimulus Source: UniProtKB
  • positive regulation of transcription by RNA polymerase II Source: MGI
  • regulation of cell cycle G2/M phase transition Source: UniProtKB
  • transcription, DNA-templated Source: UniProtKB-KW

Keywordsi

Biological processDNA damage, DNA recombination, DNA repair, Transcription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
PAXIP1-associated glutamate-rich protein 1AImported
Alternative name(s):
PAXIP1-associated protein 1
PTIP-associated protein 1
Gene namesi
Name:Pagr1aImported
Synonyms:Pa1Imported, Pagr
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:1914528. Pagr1a.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

Normal germ layer specification and developmental patterning but limited anterior development and arrest by E8.5, likely due at least in part to defects in extraembryonic tissue.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002483351 – 253PAXIP1-associated glutamate-rich protein 1ACuratedAdd BLAST253

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei137PhosphothreonineBy similarity1
Modified residuei142PhosphoserineBy similarity1
Modified residuei147PhosphoserineBy similarity1
Modified residuei236PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ99L02.
MaxQBiQ99L02.
PaxDbiQ99L02.
PeptideAtlasiQ99L02.
PRIDEiQ99L02.

PTM databases

iPTMnetiQ99L02.
PhosphoSitePlusiQ99L02.

Expressioni

Developmental stagei

Expression first detected at E5.0 to E6.0 in the extraembryonic region, at E7.5 detected within the chorion; later the expression is expanding to the entire embryo.1 Publication

Gene expression databases

BgeeiENSMUSG00000030680.
CleanExiMM_2900092E17RIK.
ExpressionAtlasiQ99L02. baseline and differential.
GenevisibleiQ99L02. MM.

Interactioni

Subunit structurei

Component of the KMT2 family MLL2/MLL3 complex, at least composed of the histone methyltransferases KMT2D and/or KMT2C, the common subunits ASH2L, RBBP5, WDR5 and DPY30, and the complex type-specific subunits PAXIP1/PTIP, PAGR1, NCOA6 and KDM6A; PAXIP1 is required for the association with the MLL2/MLL3 complex (By similarity). Forms a constitutive complex with PAXIP1/PTIP independently of the MLL2/MLL3 complex (PubMed:19124460, PubMed:26744420). Interacts with NCOA1, ESR1, NR3C1, AR (By similarity).By similarity2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
Paxip1Q6NZQ411EBI-11667455,EBI-1395317

GO - Molecular functioni

Protein-protein interaction databases

BioGridi212067. 3 interactors.
IntActiQ99L02. 1 interactor.
STRINGi10090.ENSMUSP00000032918.

Structurei

3D structure databases

ProteinModelPortaliQ99L02.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni115 – 159Sufficient for interaction with NCOA1By similarityAdd BLAST45
Regioni160 – 253Sufficient for interaction with ESR1By similarityAdd BLAST94

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi47 – 159Glu-richAdd BLAST113

Phylogenomic databases

eggNOGiENOG410IXWI. Eukaryota.
ENOG4111Q6K. LUCA.
GeneTreeiENSGT00390000016049.
HOGENOMiHOG000115419.
HOVERGENiHBG080642.
InParanoidiQ99L02.
KOiK14973.
OMAiESEDWCV.
PhylomeDBiQ99L02.
TreeFamiTF326621.

Family and domain databases

InterProiView protein in InterPro
IPR028213. PA1.
PANTHERiPTHR28467. PTHR28467. 1 hit.
PfamiView protein in Pfam
PF15364. PAXIP1_C. 1 hit.

Sequencei

Sequence statusi: Complete.

Q99L02-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSLALGHGTI AGSTAAPLSE EGEVTSGLQA LAVEDTGGPS VSASKAEEEG
60 70 80 90 100
KGSQEEAGRE GSRPEEALEA PSAASDERAE GEAEDWCVPC SDEEVELPAN
110 120 130 140 150
GQSWMPPPSE IQRLYELLAT QGTLELQAEI LPRRPPTPEA QSEEERSDEE
160 170 180 190 200
PEAKEEEEEK PHMPTEFDFD DEPMTPKDSL IDRRRTPGSS ARSQKREARL
210 220 230 240 250
DKVLSDMKRH KKLEEQILRT GRDLFSLDSE GPSPTSPPLR SSGNSLFPRQ

RKY
Length:253
Mass (Da):27,723
Last modified:June 1, 2001 - v1
Checksum:iAA5E4C4503A5C6FF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK082775 mRNA. Translation: BAC38614.1.
BC003932 mRNA. Translation: AAH03932.1.
CCDSiCCDS21854.1.
RefSeqiNP_084516.1. NM_030240.1.
UniGeneiMm.345385.

Genome annotation databases

EnsembliENSMUST00000200948; ENSMUSP00000144469; ENSMUSG00000030680.
ENSMUST00000202798; ENSMUSP00000144243; ENSMUSG00000107068.
GeneIDi67278.
KEGGimmu:67278.
UCSCiuc009jtx.1. mouse.

Similar proteinsi

Entry informationi

Entry nameiPGR1A_MOUSE
AccessioniPrimary (citable) accession number: Q99L02
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 5, 2006
Last sequence update: June 1, 2001
Last modified: March 28, 2018
This is version 104 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Caution

The terminology of MLL proteins in mammalia is not consistent also concerning the terminology of MLL protein-containing complexes. The decribed MLL2/MLL3 complex is commonly described as MLL3/MLL4 complex in literature.Curated

Keywords - Technical termi

Complete proteome, Reference proteome